O49432 (QRT3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polygalacturonase QRT3
Short name=AtQRT3
Short name=PG QRT3
EC=3.2.1.15

Alternative name(s):
Pectinase QRT3
Protein QUARTET 3

Gene names

Name:	QRT3
Ordered Locus Names:	At4g20050
ORF Names:	F18F4.150

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Polygalacturonase required for degrading the pollen mother cell wall during microspore development. Ref.1
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted › cell wall Ref.1.
Tissue specificity	Expressed in the tapetum cells in the anthers and in the ovules of open flowers. Ref.1
Developmental stage	Transiently expressed during the early microspore stage. Ref.1
Disruption phenotype	The mature pollen grains are arranged in a tetrad. A layer of material is frequently deposited on the surface of the distal region of the pollen grains. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 3 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW microsporogenesis Inferred from mutant phenotype Ref.1. Source: TAIR
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	polygalacturonase activity Inferred from direct assay Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 481	454	Polygalacturonase QRT3		PRO_0000370639
Regions
	Repeat	203 – 226	24	PbH1 1
	Repeat	261 – 282	22	PbH1 2
	Repeat	356 – 377	22	PbH1 3
Amino acid modifications
	Glycosylation	415	1	N-linked (GlcNAc...) Potential
	Glycosylation	455	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O49432 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 9299F16297364257	FASTA	481	51,659
	10 20 30 40 50 60 MELRKSQVAM PVFLAIMSLM VSQVVFAEKD SGSMSPHDRA LAEMQALKAS LVRRNLPALV 70 80 90 100 110 120 SPPPTPPQAV PGPRVYQVIS YGADPTGKLD STDAILKAME EAFDGPNHGV LMQGINDLGG 130 140 150 160 170 180 ARIDLQGGSY LISRPLRFPS AGAGNLLISG GTLRASNDFP VDRYLIELKD ESSKLQYIFE 190 200 210 220 230 240 YITLRDLLID CNYRGGAIAV INSLRTSIDN CYITRFGDTN GILVKSGHET YIRNSFLGQH 250 260 270 280 290 300 ITAGGDRGER SFSGTAINLM GNDNAVTDTV IFSARIGVMV SGQANLLSGV HCYNKATGFG 310 320 330 340 350 360 GTGIYLRLPG LTQNRIVNSY LDYTGIVAED PVQLQISGTF FLGDAFILLK SIAGYIRGVS 370 380 390 400 410 420 IVDNMFSGSG HGVQIVQLDQ RNTAFDDVGQ VVVDRNSVNG MVEKSTVARG SVDGNGTSWT 430 440 450 460 470 480 VDFNPVLLFP DLINHVQYTL VASEAGVFPL HALRNVSDNR VVVETNAPVT GTVYVTVNQG V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Microspore separation in the quartet 3 mutants of Arabidopsis is impaired by a defect in a developmentally regulated polygalacturonase required for pollen mother cell wall degradation." Rhee S.Y., Osborne E., Poindexter P.D., Somerville C.R. Plant Physiol. 133:1170-1180(2003) [PubMed: 14551328] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE. Strain: cv. Columbia and cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY268942 mRNA. Translation: AAQ83300.1. AY268941 Genomic DNA. Translation: AAQ83299.1. AL021637 Genomic DNA. Translation: CAA16613.1. AL161552 Genomic DNA. Translation: CAB79005.1. CP002687 Genomic DNA. Translation: AEE84266.1. CP002687 Genomic DNA. Translation: AEE84267.1.
IPI	IPI00548352.
PIR	T04889.
RefSeq	NP_001078410.1. NM_001084941.1. NP_193738.1. NM_118124.3.
UniGene	At.32754.
3D structure databases
ProteinModelPortal	O49432.
SMR	O49432. Positions 81-159.
ModBase	Search...
Proteomic databases
PRIDE	O49432.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G20050.1; AT4G20050.1; AT4G20050. AT4G20050.2; AT4G20050.2; AT4G20050.
GeneID	827750.
GenomeReviews	Gene locus AT4G20050 in contig CT486007_GR.
KEGG	ath:AT4G20050.
NMPDR	fig|3702.1.peg.19835.
Organism-specific databases
TAIR	At4g20050.
Phylogenomic databases
GeneTree	EPGT00050000017759.
HOGENOM	HBG318504.
InParanoid	O49432.
OMA	KSGHETY.
PhylomeDB	O49432.
ProtClustDB	CLSN2685725.
Gene expression databases
ArrayExpress	O49432.
Genevestigator	O49432.
Family and domain databases
InterPro	IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
SMART	SM00710. PbH1. 3 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QRT3_ARATH

Accession

Primary (citable) accession number: O49432

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	June 1, 1998
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program