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Protein

Cytochrome P450 82C2

Gene

CYP82C2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the biosynthetic pathway to 4-hydroxyindole-3-carbonyl nitrile (4-OH-ICN), a cyanogenic metabolite required for inducible pathogen defense. Converts indole-3-carbonyl nitrile (ICN) into 4-OH-ICN (PubMed:26352477). Can hydroxylate 8-methoxypsoralen to form 5-hydroxy-8-methoxypsoralen in vivo and in vitro (PubMed:18291319).2 Publications

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi462 – 4621Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to hypoxia Source: TAIR
  • secondary metabolite biosynthetic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT4G31970-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 82C2 (EC:1.14.-.-)
Gene namesi
Name:CYP82C2
Ordered Locus Names:At4g31970
ORF Names:F10N7.220
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G31970.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Increased susceptibility to virulent Pseudomonas syringae.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 523523Cytochrome P450 82C2PRO_0000411197Add
BLAST

Proteomic databases

PaxDbiO49394.
PRIDEiO49394.

Expressioni

Inductioni

Up-regulated upon pathogen infection.1 Publication

Gene expression databases

GenevisibleiO49394. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G31970.1.

Structurei

3D structure databases

ProteinModelPortaliO49394.
SMRiO49394. Positions 61-516.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000218627.
InParanoidiO49394.
OMAiANFFHLV.
OrthoDBiEOG0936087W.
PhylomeDBiO49394.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O49394-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTSLFSLFV PILVFVFIAL FKKSKKPKHV KAPAPSGAWP IIGHLHLLSG
60 70 80 90 100
KEQLLYRTLG KMADQYGPAM SLRLGSSETF VVSSFEVAKD CFTVNDKALA
110 120 130 140 150
SRPITAAAKH MGYDCAVFGF APYSAFWREM RKIATLELLS NRRLQMLKHV
160 170 180 190 200
RVSEISMVMQ DLYSLWVKKG GSEPVMVDLK SWLEDMSLNM MVRMVAGKRY
210 220 230 240 250
FGGGSLSPED AEEARQCRKG VANFFHLVGI FTVSDAFPKL GWFDFQGHEK
260 270 280 290 300
EMKQTGRELD VILERWIENH RQQRKVSGTK HNDSDFVDVM LSLAEQGKFS
310 320 330 340 350
HLQHDAITSI KSTCLALILG GSETSPSTLT WAISLLLNNK DMLKKAQDEI
360 370 380 390 400
DIHVGRDRNV EDSDIENLVY IQAIIKETLR LYPAGPLLGH REAIEDCTVA
410 420 430 440 450
GYNVRRGTRM LVNVWKIQRD PRVYMEPNEF RPERFITGEA KEFDVRGQNF
460 470 480 490 500
ELMPFGSGRR SCPGSSLAMQ VLHLGLARFL QSFDVKTVMD MPVDMTESPG
510 520
LTIPKATPLE ILISPRLKEG LYV
Length:523
Mass (Da):59,002
Last modified:January 1, 1999 - v2
Checksum:i0FE5D79217DFDAA9
GO

Sequence cautioni

The sequence ABK28662 differs from that shown. Reason: Erroneous termination at position 524. Translated as stop.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL021636 Genomic DNA. Translation: CAA16592.1.
AL161580 Genomic DNA. Translation: CAB79915.1.
CP002687 Genomic DNA. Translation: AEE85984.1.
DQ653241 mRNA. Translation: ABK28662.1. Sequence problems.
PIRiT04648.
RefSeqiNP_194925.1. NM_119348.1.
UniGeneiAt.49411.

Genome annotation databases

EnsemblPlantsiAT4G31970.1; AT4G31970.1; AT4G31970.
GeneIDi829327.
GrameneiAT4G31970.1; AT4G31970.1; AT4G31970.
KEGGiath:AT4G31970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL021636 Genomic DNA. Translation: CAA16592.1.
AL161580 Genomic DNA. Translation: CAB79915.1.
CP002687 Genomic DNA. Translation: AEE85984.1.
DQ653241 mRNA. Translation: ABK28662.1. Sequence problems.
PIRiT04648.
RefSeqiNP_194925.1. NM_119348.1.
UniGeneiAt.49411.

3D structure databases

ProteinModelPortaliO49394.
SMRiO49394. Positions 61-516.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G31970.1.

Proteomic databases

PaxDbiO49394.
PRIDEiO49394.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G31970.1; AT4G31970.1; AT4G31970.
GeneIDi829327.
GrameneiAT4G31970.1; AT4G31970.1; AT4G31970.
KEGGiath:AT4G31970.

Organism-specific databases

TAIRiAT4G31970.

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000218627.
InParanoidiO49394.
OMAiANFFHLV.
OrthoDBiEOG0936087W.
PhylomeDBiO49394.

Enzyme and pathway databases

BioCyciARA:AT4G31970-MONOMER.

Miscellaneous databases

PROiO49394.

Gene expression databases

GenevisibleiO49394. AT.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC82C2_ARATH
AccessioniPrimary (citable) accession number: O49394
Secondary accession number(s): A0MFB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: January 1, 1999
Last modified: September 7, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants overexpressing CYP82C2, can hydroxylate and subsequently glycosylate 8-methoxypsoralen.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.