Indoleacetaldoxime dehydratase - Arabidopsis thaliana (Mouse-ear cress)

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O49342 (C71AD_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Indoleacetaldoxime dehydratase
EC=4.99.1.6

Alternative name(s):
Cytochrome P450 71A13

Gene names

Name:	CYP71A13
Ordered Locus Names:	At2g30770
ORF Names:	T11J7.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the biosynthesis of the indole-derived phytoalexin camalexin. Catalyzes the conversion of indole-3-acetaldoxime to indole-3-acetonitrile. Required for resistance to A.brassicicola and B.cinerea. Ref.4 Ref.7
Catalytic activity	(Indol-3-yl)acetaldehyde oxime = (indol-3-yl)acetonitrile + H₂O. Ref.4
Subcellular location	Membrane; Single-pass membrane protein Potential.
Induction	By Pseudomonas syringae pv. tomato DC3000, Botrytis cinerea, flagellin, BTH and UV-C. Repressed by the transcription factors WRKY18 and WRKY40 upon infection with Golovinomyces orontii. Ref.5 Ref.6 Ref.7 Ref.8
Sequence similarities	Belongs to the cytochrome P450 family.
Sequence caution	The sequence AEC08439.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro indoleacetaldoxime dehydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 497

497

Indoleacetaldoxime dehydratase

PRO_0000052064

Regions

Transmembrane

2 – 20

Helical; Potential

Compositional bias

164 – 169

Poly-Ser

Sequences

Sequence

Length

Mass (Da)

Tools

O49342 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 3C8CE0773624B59B
Show »

FASTA

497

56,086

        10         20         30         40         50         60 
MEMILSISLC LTTLITLLLL RRFLKRTATK VNLPPSPWRL PVIGNLHQLS LHPHRSLRSL 

        70         80         90        100        110        120 
SLRYGPLMLL HFGRVPILVV SSGEAAQEVL KTHDHKFANR PRSKAVHGLM NGGRDVVFAP 

       130        140        150        160        170        180 
YGEYWRQMKS VCILNLLTNK MVESFEKVRE DEVNAMIEKL EKASSSSSSE NLSELFITLP 

       190        200        210        220        230        240 
SDVTSRVALG RKHSEDETAR DLKKRVRQIM ELLGEFPIGE YVPILAWIDG IRGFNNKIKE 

       250        260        270        280        290        300 
VSRGFSDLMD KVVQEHLEAS NDKADFVDIL LSIEKDKNSG FQVQRNDIKF MILDMFIGGT 

       310        320        330        340        350        360 
STTSTLLEWT MTELIRSPKS MKKLQDEIRS TIRPHGSYIK EKEVENMKYL KAVIKEVLRL 

       370        380        390        400        410        420 
HPSLPMILPR LLSEDVKVKG YNIAAGTEVI INAWAIQRDT AIWGPDAEEF KPERHLDSGL 

       430        440        450        460        470        480 
DYHGKNLNYI PFGSGRRICP GINLALGLAE VTVANLVGRF DWRVEAGPNG DQPDLTEAIG 

       490 
IDVCRKFPLI AFPSSVV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation." Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M. Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis cytochrome P450 monooxygenase 71A13 catalyzes the conversion of indole-3-acetaldoxime in camalexin synthesis." Nafisi M., Goregaoker S., Botanga C.J., Glawischnig E., Olsen C.E., Halkier B.A., Glazebrook J. Plant Cell 19:2039-2052(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.
[5]	"Arabidopsis MAP kinase 4 regulates gene expression through transcription factor release in the nucleus." Qiu J.L., Fiil B.K., Petersen K., Nielsen H.B., Botanga C.J., Thorgrimsen S., Palma K., Suarez-Rodriguez M.C., Sandbech-Clausen S., Lichota J., Brodersen P., Grasser K.D., Mattsson O., Glazebrook J., Mundy J., Petersen M. EMBO J. 27:2214-2221(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[6]	"Glycerol-3-phosphate levels are associated with basal resistance to the hemibiotrophic fungus Colletotrichum higginsianum in Arabidopsis." Chanda B., Venugopal S.C., Kulshrestha S., Navarre D.A., Downie B., Vaillancourt L., Kachroo A., Kachroo P. Plant Physiol. 147:2017-2029(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[7]	"The ABC transporter BcatrB from Botrytis cinerea exports camalexin and is a virulence factor on Arabidopsis thaliana." Stefanato F.L., Abou-Mansour E., Buchala A., Kretschmer M., Mosbach A., Hahn M., Bochet C.G., Metraux J.P., Schoonbeek H.J. Plant J. 58:499-510(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[8]	"Transcriptional reprogramming regulated by WRKY18 and WRKY40 facilitates powdery mildew infection of Arabidopsis." Pandey S.P., Roccaro M., Schoen M., Logemann E., Somssich I.E. Plant J. 64:912-923(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002340 Genomic DNA. Translation: AAC02748.1. CP002685 Genomic DNA. Translation: AEC08439.1. Different initiation. BX820453 mRNA. No translation available.
IPI	IPI00527969.
PIR	E84712.
RefSeq	NP_180635.2. NM_128630.2.
UniGene	At.20309. At.68697.
3D structure databases
ProteinModelPortal	O49342.
SMR	O49342. Positions 33-495.
ModBase	Search...
Protein-protein interaction databases
IntAct	O49342. 1 interaction.
STRING	3702.AT2G30770.1-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	817628.
KEGG	ath:AT2G30770.
Organism-specific databases
GeneFarm	1175. 94.
TAIR	At2g30770.
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000218629.
InParanoid	O49342.
KO	K11868.
PhylomeDB	O49342.
Enzyme and pathway databases
BioCyc	ARA:AT2G30770-MONOMER. MetaCyc:AT2G30770-MONOMER.
Gene expression databases
Genevestigator	O49342.
GermOnline	AT2G30770. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C71AD_ARATH

Accession

Primary (citable) accession number: O49342
Secondary accession number(s): F4INY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents