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Protein

Cytochrome P450 71A12

Gene

CYP71A12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Converts indole-3-acetaldoxime to indole cyanohydrin. Involved in the biosynthetic pathway to 4-hydroxyindole-3-carbonyl nitrile (4-OH-ICN), a cyanogenic metabolite required for inducible pathogen defense.1 Publication

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi439 – 4391Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 71A12 (EC:1.14.-.-Curated)
Gene namesi
Name:CYP71A12
Ordered Locus Names:At2g30750
ORF Names:T11J7.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G30750.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

90% decrease of all indole-3-carbonyl nitrile (ICN) derivatives and increased susceptibility to virulent Pseudomonas syringae.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497Cytochrome P450 71A12PRO_0000052063Add
BLAST

Proteomic databases

PaxDbiO49340.
PRIDEiO49340.

Expressioni

Gene expression databases

ExpressionAtlasiO49340. baseline and differential.
GenevisibleiO49340. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G30750.1.

Structurei

3D structure databases

ProteinModelPortaliO49340.
SMRiO49340. Positions 30-494.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi164 – 1696Poly-Ser

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000218629.
InParanoidiO49340.
KOiK00517.
PhylomeDBiO49340.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O49340-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMILMVSLC LTTLITLFLL KQFLKRTANK VNLPPSPWRL PLIGNLHQLS
60 70 80 90 100
LHPHRSLHSL SLRYGPLMLL HFGRVPILVV SSGEAAQEVL KTHDLKFANR
110 120 130 140 150
PRSKAVHGLM NGGRDVVFGP YGEYWRQMKS VCILNLLTNK MVASFEKIRE
160 170 180 190 200
EELNEMIKKL EKASSSSSSE NLSELFVTLP SDVTSRIALG RKHSEDETAR
210 220 230 240 250
DLKKRVRQIM ELLGEFPIGD YVPALAWIDR INGFNARIKE VSQGFSDLMD
260 270 280 290 300
KVVQEHLEAG NHKEDFVDIL LSIESEKSIG FQAQRDDIKF MILDMFIGGT
310 320 330 340 350
STSSTLLEWI MTELIRNPNV MKKLQDEIRS TIRPHGSYIK EKDVENMKYL
360 370 380 390 400
KAVIKEVFRV HPPLPLILPR LLSEDVKVKG YNIAAGTEVI INAWAIQRDP
410 420 430 440 450
AIWGPDAEEF KPERHLDSTL DYHGKDLNFI PFGSGRRICP GINLALGLVE
460 470 480 490
VTVANLVGRF DWRAEAGPNG DQPDLTEAFG LDVCRKFPLI AFPSSVI
Length:497
Mass (Da):56,152
Last modified:June 1, 1998 - v1
Checksum:iBE6AC1A28A737C81
GO

Sequence cautioni

The sequence AAM19850.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAN46800.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AEC08435.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002340 Genomic DNA. Translation: AAC02746.1.
CP002685 Genomic DNA. Translation: AEC08435.1. Different initiation.
AY094484 mRNA. Translation: AAM19850.1. Different initiation.
BT001046 mRNA. Translation: AAN46800.1. Different initiation.
PIRiC84712.
RefSeqiNP_180633.2. NM_128628.3.
UniGeneiAt.38323.

Genome annotation databases

EnsemblPlantsiAT2G30750.1; AT2G30750.1; AT2G30750.
GeneIDi817626.
GrameneiAT2G30750.1; AT2G30750.1; AT2G30750.
KEGGiath:AT2G30750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002340 Genomic DNA. Translation: AAC02746.1.
CP002685 Genomic DNA. Translation: AEC08435.1. Different initiation.
AY094484 mRNA. Translation: AAM19850.1. Different initiation.
BT001046 mRNA. Translation: AAN46800.1. Different initiation.
PIRiC84712.
RefSeqiNP_180633.2. NM_128628.3.
UniGeneiAt.38323.

3D structure databases

ProteinModelPortaliO49340.
SMRiO49340. Positions 30-494.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G30750.1.

Proteomic databases

PaxDbiO49340.
PRIDEiO49340.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G30750.1; AT2G30750.1; AT2G30750.
GeneIDi817626.
GrameneiAT2G30750.1; AT2G30750.1; AT2G30750.
KEGGiath:AT2G30750.

Organism-specific databases

TAIRiAT2G30750.

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000218629.
InParanoidiO49340.
KOiK00517.
PhylomeDBiO49340.

Miscellaneous databases

PROiO49340.

Gene expression databases

ExpressionAtlasiO49340. baseline and differential.
GenevisibleiO49340. AT.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "A new cyanogenic metabolite in Arabidopsis required for inducible pathogen defence."
    Rajniak J., Barco B., Clay N.K., Sattely E.S.
    Nature 525:376-379(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiC71AC_ARATH
AccessioniPrimary (citable) accession number: O49340
Secondary accession number(s): F4INX7, Q8LPP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: June 1, 1998
Last modified: July 6, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.