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Protein

PTI1-like tyrosine-protein kinase 2

Gene

PTI12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable tyrosine-protein kinase involved in oxidative burst-mediated signaling leading to specific genes expression.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Enzyme regulationi

Strongly activated in response to phosphatidic acid (PA) and xylanase in a OXI1- and PDK1-dependent manner, and, to a lesser extent, by hydrogen peroxide and flagellin in a OXI1-dependent manner.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991ATPCurated
Active sitei203 – 2031Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi77 – 859ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  3. protein kinase binding Source: UniProtKB

GO - Biological processi

  1. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Plant defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G30740-MONOMER.
ReactomeiREACT_274451. activated TAK1 mediates p38 MAPK activation.
REACT_282733. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.

Names & Taxonomyi

Protein namesi
Recommended name:
PTI1-like tyrosine-protein kinase 2 (EC:2.7.10.2)
Short name:
PTI1-2
Gene namesi
Name:PTI12
Ordered Locus Names:At2g30740
ORF Names:T11J7.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G30740.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: TAIR
  2. plasmodesma Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi99 – 991K → N: Reduced phosphorylation and impaired kinase activity. No phosphorylation; when associated with A-238. 1 Publication
Mutagenesisi238 – 2381T → A: No phosphorylation; when associated with N-99. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366PTI1-like tyrosine-protein kinase 2PRO_0000403323Add
BLAST

Post-translational modificationi

Autophosphorylated and phosphorylated by OXI1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO49339.
PRIDEiO49339.

Expressioni

Gene expression databases

GenevestigatoriO49339.

Interactioni

Subunit structurei

Interacts with OXI1.1 Publication

Protein-protein interaction databases

BioGridi2974. 2 interactions.
STRINGi3702.AT2G30740.1-P.

Structurei

3D structure databases

ProteinModelPortaliO49339.
SMRiO49339. Positions 54-353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini71 – 353283Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000116550.
InParanoidiO49339.
OMAiWICCNCQ.
PhylomeDBiO49339.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O49339-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRWICCGDK KGDSDLSNEE VHLKSPWQNS EANQKNQKPQ AVVKPEAQKE
60 70 80 90 100
ALPIEVPPLS VDEVKEKTDN FGSKSLIGEG SYGRVYYATL NDGKAVALKK
110 120 130 140 150
LDVAPEAETN TEFLNQVSMV SRLKHENLIQ LVGYCVDENL RVLAYEFATM
160 170 180 190 200
GSLHDILHGR KGVQGAQPGP TLDWLTRVKI AVEAARGLEY LHEKVQPPVI
210 220 230 240 250
HRDIRSSNVL LFEDYQAKVA DFNLSNQAPD NAARLHSTRV LGTFGYHAPE
260 270 280 290 300
YAMTGQLTQK SDVYSFGVVL LELLTGRKPV DHTMPRGQQS LVTWATPRLS
310 320 330 340 350
EDKVKQCVDP KLKGEYPPKS VAKLAAVAAL CVQYESEFRP NMSIVVKALQ
360
PLLKPPAPAP APVPES
Length:366
Mass (Da):40,500
Last modified:June 1, 1998 - v1
Checksum:iE9A0450069D35C72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303007 mRNA. Translation: CAC34450.1.
AC002340 Genomic DNA. Translation: AAC02745.1.
CP002685 Genomic DNA. Translation: AEC08434.1.
BT024893 mRNA. Translation: ABD85164.1.
AK230305 mRNA. Translation: BAF02106.1.
PIRiB84712.
RefSeqiNP_180632.1. NM_128627.2.
UniGeneiAt.20728.

Genome annotation databases

EnsemblPlantsiAT2G30740.1; AT2G30740.1; AT2G30740.
GeneIDi817625.
KEGGiath:AT2G30740.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC
Arabidopsis protein tyrosine kinases

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303007 mRNA. Translation: CAC34450.1.
AC002340 Genomic DNA. Translation: AAC02745.1.
CP002685 Genomic DNA. Translation: AEC08434.1.
BT024893 mRNA. Translation: ABD85164.1.
AK230305 mRNA. Translation: BAF02106.1.
PIRiB84712.
RefSeqiNP_180632.1. NM_128627.2.
UniGeneiAt.20728.

3D structure databases

ProteinModelPortaliO49339.
SMRiO49339. Positions 54-353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2974. 2 interactions.
STRINGi3702.AT2G30740.1-P.

Proteomic databases

PaxDbiO49339.
PRIDEiO49339.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G30740.1; AT2G30740.1; AT2G30740.
GeneIDi817625.
KEGGiath:AT2G30740.

Organism-specific databases

GeneFarmi1792. 139.
TAIRiAT2G30740.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000116550.
InParanoidiO49339.
OMAiWICCNCQ.
PhylomeDBiO49339.

Enzyme and pathway databases

BioCyciARA:AT2G30740-MONOMER.
ReactomeiREACT_274451. activated TAK1 mediates p38 MAPK activation.
REACT_282733. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.

Gene expression databases

GenevestigatoriO49339.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comparative proteome bioinformatics: identification of a whole complement of putative protein tyrosine kinases in the model flowering plant Arabidopsis thaliana."
    Carpi A., Di Maira G., Vedovato M., Rossi V., Naccari T., Floriduz M., Terzi M., Filippini F.
    Proteomics 2:1494-1503(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Shinn P., Chen H., Kim C.J., Ecker J.R.
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "The Arabidopsis protein kinase PTI1-2 is activated by convergent phosphatidic acid and oxidative stress signaling pathways downstream of PDK1 and OXI1."
    Anthony R.G., Khan S., Costa J., Pais M.S., Boegre L.
    J. Biol. Chem. 281:37536-37546(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH OXI1, AUTOPHOSPHORYLATION, PHOSPHORYLATION BY OXI1, MUTAGENESIS OF LYS-99 AND THR-238, ENZYME REGULATION.

Entry informationi

Entry nameiPTI12_ARATH
AccessioniPrimary (citable) accession number: O49339
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: June 1, 1998
Last modified: April 1, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.