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O49339

- PTI12_ARATH

UniProt

O49339 - PTI12_ARATH

Protein

PTI1-like tyrosine-protein kinase 2

Gene

PTI12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Probable tyrosine-protein kinase involved in oxidative burst-mediated signaling leading to specific genes expression.1 Publication

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Enzyme regulationi

    Strongly activated in response to phosphatidic acid (PA) and xylanase in a OXI1- and PDK1-dependent manner, and, to a lesser extent, by hydrogen peroxide and flagellin in a OXI1-dependent manner.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei99 – 991ATPCurated
    Active sitei203 – 2031Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi77 – 859ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    3. protein kinase binding Source: UniProtKB

    GO - Biological processi

    1. defense response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Plant defense

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G30740-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    PTI1-like tyrosine-protein kinase 2 (EC:2.7.10.2)
    Short name:
    PTI1-2
    Gene namesi
    Name:PTI12
    Ordered Locus Names:At2g30740
    ORF Names:T11J7.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G30740.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: TAIR
    2. plasmodesma Source: TAIR

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi99 – 991K → N: Reduced phosphorylation and impaired kinase activity. No phosphorylation; when associated with A-238. 1 Publication
    Mutagenesisi238 – 2381T → A: No phosphorylation; when associated with N-99. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 366366PTI1-like tyrosine-protein kinase 2PRO_0000403323Add
    BLAST

    Post-translational modificationi

    Autophosphorylated and phosphorylated by OXI1.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO49339.
    PRIDEiO49339.

    Expressioni

    Gene expression databases

    GenevestigatoriO49339.

    Interactioni

    Subunit structurei

    Interacts with OXI1.1 Publication

    Protein-protein interaction databases

    BioGridi2974. 2 interactions.
    STRINGi3702.AT2G30740.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO49339.
    SMRiO49339. Positions 56-353.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini71 – 353283Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000116550.
    InParanoidiO49339.
    OMAiPYLENEN.
    PhylomeDBiO49339.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008266. Tyr_kinase_AS.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O49339-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRRWICCGDK KGDSDLSNEE VHLKSPWQNS EANQKNQKPQ AVVKPEAQKE    50
    ALPIEVPPLS VDEVKEKTDN FGSKSLIGEG SYGRVYYATL NDGKAVALKK 100
    LDVAPEAETN TEFLNQVSMV SRLKHENLIQ LVGYCVDENL RVLAYEFATM 150
    GSLHDILHGR KGVQGAQPGP TLDWLTRVKI AVEAARGLEY LHEKVQPPVI 200
    HRDIRSSNVL LFEDYQAKVA DFNLSNQAPD NAARLHSTRV LGTFGYHAPE 250
    YAMTGQLTQK SDVYSFGVVL LELLTGRKPV DHTMPRGQQS LVTWATPRLS 300
    EDKVKQCVDP KLKGEYPPKS VAKLAAVAAL CVQYESEFRP NMSIVVKALQ 350
    PLLKPPAPAP APVPES 366
    Length:366
    Mass (Da):40,500
    Last modified:June 1, 1998 - v1
    Checksum:iE9A0450069D35C72
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ303007 mRNA. Translation: CAC34450.1.
    AC002340 Genomic DNA. Translation: AAC02745.1.
    CP002685 Genomic DNA. Translation: AEC08434.1.
    BT024893 mRNA. Translation: ABD85164.1.
    AK230305 mRNA. Translation: BAF02106.1.
    PIRiB84712.
    RefSeqiNP_180632.1. NM_128627.2.
    UniGeneiAt.20728.

    Genome annotation databases

    EnsemblPlantsiAT2G30740.1; AT2G30740.1; AT2G30740.
    GeneIDi817625.
    KEGGiath:AT2G30740.

    Cross-referencesi

    Web resourcesi

    PlantP kinase Classification PPC
    Arabidopsis protein tyrosine kinases

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ303007 mRNA. Translation: CAC34450.1 .
    AC002340 Genomic DNA. Translation: AAC02745.1 .
    CP002685 Genomic DNA. Translation: AEC08434.1 .
    BT024893 mRNA. Translation: ABD85164.1 .
    AK230305 mRNA. Translation: BAF02106.1 .
    PIRi B84712.
    RefSeqi NP_180632.1. NM_128627.2.
    UniGenei At.20728.

    3D structure databases

    ProteinModelPortali O49339.
    SMRi O49339. Positions 56-353.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 2974. 2 interactions.
    STRINGi 3702.AT2G30740.1-P.

    Proteomic databases

    PaxDbi O49339.
    PRIDEi O49339.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G30740.1 ; AT2G30740.1 ; AT2G30740 .
    GeneIDi 817625.
    KEGGi ath:AT2G30740.

    Organism-specific databases

    GeneFarmi 1792. 139.
    TAIRi AT2G30740.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000116550.
    InParanoidi O49339.
    OMAi PYLENEN.
    PhylomeDBi O49339.

    Enzyme and pathway databases

    BioCyci ARA:AT2G30740-MONOMER.

    Gene expression databases

    Genevestigatori O49339.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008266. Tyr_kinase_AS.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative proteome bioinformatics: identification of a whole complement of putative protein tyrosine kinases in the model flowering plant Arabidopsis thaliana."
      Carpi A., Di Maira G., Vedovato M., Rossi V., Naccari T., Floriduz M., Terzi M., Filippini F.
      Proteomics 2:1494-1503(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Shinn P., Chen H., Kim C.J., Ecker J.R.
      Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "The Arabidopsis protein kinase PTI1-2 is activated by convergent phosphatidic acid and oxidative stress signaling pathways downstream of PDK1 and OXI1."
      Anthony R.G., Khan S., Costa J., Pais M.S., Boegre L.
      J. Biol. Chem. 281:37536-37546(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH OXI1, AUTOPHOSPHORYLATION, PHOSPHORYLATION BY OXI1, MUTAGENESIS OF LYS-99 AND THR-238, ENZYME REGULATION.

    Entry informationi

    Entry nameiPTI12_ARATH
    AccessioniPrimary (citable) accession number: O49339
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2011
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3