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Protein

PTI1-like tyrosine-protein kinase 2

Gene

PTI12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable tyrosine-protein kinase involved in oxidative burst-mediated signaling leading to specific genes expression.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Enzyme regulationi

Strongly activated in response to phosphatidic acid (PA) and xylanase in a OXI1- and PDK1-dependent manner, and, to a lesser extent, by hydrogen peroxide and flagellin in a OXI1-dependent manner.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99ATPCurated1
Active sitei203Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 85ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Plant defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-450302. activated TAK1 mediates p38 MAPK activation.
R-ATH-450321. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.

Names & Taxonomyi

Protein namesi
Recommended name:
PTI1-like tyrosine-protein kinase 2 (EC:2.7.10.2)
Short name:
PTI1-2
Gene namesi
Name:PTI12
Ordered Locus Names:At2g30740
ORF Names:T11J7.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G30740.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi99K → N: Reduced phosphorylation and impaired kinase activity. No phosphorylation; when associated with A-238. 1 Publication1
Mutagenesisi238T → A: No phosphorylation; when associated with N-99. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004033231 – 366PTI1-like tyrosine-protein kinase 2Add BLAST366

Post-translational modificationi

Autophosphorylated and phosphorylated by OXI1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO49339.
PRIDEiO49339.

PTM databases

iPTMnetiO49339.
SwissPalmiO49339.

Expressioni

Gene expression databases

GenevisibleiO49339. AT.

Interactioni

Subunit structurei

Interacts with OXI1.1 Publication

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi2974. 2 interactors.
STRINGi3702.AT2G30740.1.

Structurei

3D structure databases

ProteinModelPortaliO49339.
SMRiO49339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini71 – 353Protein kinasePROSITE-ProRule annotationAdd BLAST283

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiO49339.
OMAiHLKSPWQ.
OrthoDBiEOG09360DYR.
PhylomeDBiO49339.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O49339-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRWICCGDK KGDSDLSNEE VHLKSPWQNS EANQKNQKPQ AVVKPEAQKE
60 70 80 90 100
ALPIEVPPLS VDEVKEKTDN FGSKSLIGEG SYGRVYYATL NDGKAVALKK
110 120 130 140 150
LDVAPEAETN TEFLNQVSMV SRLKHENLIQ LVGYCVDENL RVLAYEFATM
160 170 180 190 200
GSLHDILHGR KGVQGAQPGP TLDWLTRVKI AVEAARGLEY LHEKVQPPVI
210 220 230 240 250
HRDIRSSNVL LFEDYQAKVA DFNLSNQAPD NAARLHSTRV LGTFGYHAPE
260 270 280 290 300
YAMTGQLTQK SDVYSFGVVL LELLTGRKPV DHTMPRGQQS LVTWATPRLS
310 320 330 340 350
EDKVKQCVDP KLKGEYPPKS VAKLAAVAAL CVQYESEFRP NMSIVVKALQ
360
PLLKPPAPAP APVPES
Length:366
Mass (Da):40,500
Last modified:June 1, 1998 - v1
Checksum:iE9A0450069D35C72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303007 mRNA. Translation: CAC34450.1.
AC002340 Genomic DNA. Translation: AAC02745.1.
CP002685 Genomic DNA. Translation: AEC08434.1.
BT024893 mRNA. Translation: ABD85164.1.
AK230305 mRNA. Translation: BAF02106.1.
PIRiB84712.
UniGeneiAt.20728.

Genome annotation databases

EnsemblPlantsiAT2G30740.1; AT2G30740.1; AT2G30740.
GrameneiAT2G30740.1; AT2G30740.1; AT2G30740.
KEGGiath:AT2G30740.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC
Arabidopsis protein tyrosine kinases

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ303007 mRNA. Translation: CAC34450.1.
AC002340 Genomic DNA. Translation: AAC02745.1.
CP002685 Genomic DNA. Translation: AEC08434.1.
BT024893 mRNA. Translation: ABD85164.1.
AK230305 mRNA. Translation: BAF02106.1.
PIRiB84712.
UniGeneiAt.20728.

3D structure databases

ProteinModelPortaliO49339.
SMRiO49339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2974. 2 interactors.
STRINGi3702.AT2G30740.1.

PTM databases

iPTMnetiO49339.
SwissPalmiO49339.

Proteomic databases

PaxDbiO49339.
PRIDEiO49339.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G30740.1; AT2G30740.1; AT2G30740.
GrameneiAT2G30740.1; AT2G30740.1; AT2G30740.
KEGGiath:AT2G30740.

Organism-specific databases

TAIRiAT2G30740.

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiO49339.
OMAiHLKSPWQ.
OrthoDBiEOG09360DYR.
PhylomeDBiO49339.

Enzyme and pathway databases

ReactomeiR-ATH-450302. activated TAK1 mediates p38 MAPK activation.
R-ATH-450321. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.

Miscellaneous databases

PROiO49339.

Gene expression databases

GenevisibleiO49339. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTI12_ARATH
AccessioniPrimary (citable) accession number: O49339
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.