O49296 (GUN4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 4
EC=3.2.1.4

Alternative name(s):
Endo-1,4-beta glucanase 4

Gene names

Ordered Locus Names:	At1g23210
ORF Names:	F26F24.6, T26J12.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 489	464	Endoglucanase 4		PRO_0000249257
Sites
	Active site	409	1	By similarity
	Active site	460	1	By similarity
	Active site	469	1	By similarity
Amino acid modifications
	Glycosylation	453	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O49296 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 9B46F1C3111C4D0B	FASTA	489	54,630
	10 20 30 40 50 60 MAGKSFMTPA IMLAMLLLIS PETYAGHDYR DALRKSILFF EGQRSGKLPP DQRLKWRRDS 70 80 90 100 110 120 ALRDGSSAGV DLTGGYYDAG DNVKFGFPMA FTTTMMSWSV IDFGKTMGPE LENAVKAIKW 130 140 150 160 170 180 GTDYLMKATQ IPDVVFVQVG DAYSDHNCWE RPEDMDTLRT VYKIDKDHSG SEVAGETAAA 190 200 210 220 230 240 LAAASIVFEK RDPVYSKMLL DRATRVFAFA QKYRGAYSDS LYQAVCPFYC DFNGYEDELL 250 260 270 280 290 300 WGAAWLHKAS KKRVYREFIV KNQVILRAGD TIHEFGWDNK HAGINVLVSK MVLMGKAEYF 310 320 330 340 350 360 QSFKQNADEF ICSLLPGISH PQVQYSQGGL LVKSGGSNMQ HVTSLSFLLL TYSNYLSHAN 370 380 390 400 410 420 KVVPCGEFTA SPALLRQVAK RQVDYILGDN PMKMSYMVGY GSRFPQKIHH RGSSVPSVVD 430 440 450 460 470 480 HPDRIGCKDG SRYFFSNNPN PNLLIGAVVG GPNITDDFPD SRPYFQLTEP TTYINAPLLG LLGYFSAHY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002311 Genomic DNA. Translation: AAC00616.1. AC005292 Genomic DNA. Translation: AAF86995.1. CP002684 Genomic DNA. Translation: AEE30357.1. DQ056459 mRNA. Translation: AAY78616.1. BT026379 mRNA. Translation: ABH04486.1.
IPI	IPI00521599.
PIR	E86366.
RefSeq	NP_173735.1. NM_102170.1.
UniGene	At.51731.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	O49296.
SMR	O49296. Positions 28-489.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G23210.1-P.
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G23210.1; AT1G23210.1; AT1G23210.
GeneID	838930.
KEGG	ath:AT1G23210.
Organism-specific databases
TAIR	At1g23210.
Phylogenomic databases
eggNOG	NOG299923.
HOGENOM	HOG000021033.
InParanoid	O49296.
OMA	RSFRENA.
PhylomeDB	O49296.
ProtClustDB	PLN02308.
Gene expression databases
Genevestigator	O49296.
GermOnline	AT1G23210. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN4_ARATH

Accession

Primary (citable) accession number: O49296

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program