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O49296 (GUN4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 4

EC=3.2.1.4
Alternative name(s):
Endo-1,4-beta glucanase 4
Gene names
Ordered Locus Names:At1g23210
ORF Names:F26F24.6, T26J12.2
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length489 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cell wall biogenesis/degradation
Cellulose degradation
Polysaccharide degradation
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 489464Endoglucanase 4
PRO_0000249257

Sites

Active site4091 By similarity
Active site4601 By similarity
Active site4691 By similarity

Amino acid modifications

Glycosylation4531N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O49296 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 9B46F1C3111C4D0B

FASTA48954,630
        10         20         30         40         50         60 
MAGKSFMTPA IMLAMLLLIS PETYAGHDYR DALRKSILFF EGQRSGKLPP DQRLKWRRDS 

        70         80         90        100        110        120 
ALRDGSSAGV DLTGGYYDAG DNVKFGFPMA FTTTMMSWSV IDFGKTMGPE LENAVKAIKW 

       130        140        150        160        170        180 
GTDYLMKATQ IPDVVFVQVG DAYSDHNCWE RPEDMDTLRT VYKIDKDHSG SEVAGETAAA 

       190        200        210        220        230        240 
LAAASIVFEK RDPVYSKMLL DRATRVFAFA QKYRGAYSDS LYQAVCPFYC DFNGYEDELL 

       250        260        270        280        290        300 
WGAAWLHKAS KKRVYREFIV KNQVILRAGD TIHEFGWDNK HAGINVLVSK MVLMGKAEYF 

       310        320        330        340        350        360 
QSFKQNADEF ICSLLPGISH PQVQYSQGGL LVKSGGSNMQ HVTSLSFLLL TYSNYLSHAN 

       370        380        390        400        410        420 
KVVPCGEFTA SPALLRQVAK RQVDYILGDN PMKMSYMVGY GSRFPQKIHH RGSSVPSVVD 

       430        440        450        460        470        480 
HPDRIGCKDG SRYFFSNNPN PNLLIGAVVG GPNITDDFPD SRPYFQLTEP TTYINAPLLG 


LLGYFSAHY 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D.
Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family."
Libertini E., Li Y., McQueen-Mason S.J.
J. Mol. Evol. 58:506-515(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC002311 Genomic DNA. Translation: AAC00616.1.
AC005292 Genomic DNA. Translation: AAF86995.1.
CP002684 Genomic DNA. Translation: AEE30357.1.
DQ056459 mRNA. Translation: AAY78616.1.
BT026379 mRNA. Translation: ABH04486.1.
PIRE86366.
RefSeqNP_173735.1. NM_102170.1.
UniGeneAt.51731.

3D structure databases

ProteinModelPortalO49296.
SMRO49296. Positions 28-489.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT1G23210.1-P.

Protein family/group databases

CAZyGH9. Glycoside Hydrolase Family 9.

Proteomic databases

PRIDEO49296.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G23210.1; AT1G23210.1; AT1G23210.
GeneID838930.
KEGGath:AT1G23210.

Organism-specific databases

TAIRAT1G23210.

Phylogenomic databases

eggNOGNOG299923.
HOGENOMHOG000021033.
InParanoidO49296.
OMARSFRENA.
PhylomeDBO49296.

Enzyme and pathway databases

BioCycARA:AT1G23210-MONOMER.

Gene expression databases

GenevestigatorO49296.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_AS.
[Graphical view]
PfamPF00759. Glyco_hydro_9. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
PROSITEPS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.
PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN4_ARATH
AccessionPrimary (citable) accession number: O49296
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: June 1, 1998
Last modified: June 11, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names