O49279 (SKI15_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SKP1-interacting partner 15

Alternative name(s):
F-box only protein 3

Gene names

Name:	SKIP15
Synonyms:	FBX3
Ordered Locus Names:	At1g76920
ORF Names:	F22K20.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of SCF(ASK-cullin-F-box) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins By similarity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Part of a SCF (ASK-cullin-F-box) protein ligase complex By similarity. Interacts with SKP1A/ASK1, SKP1B/ASK2, ASK11 and ASK13. Ref.6 Ref.7
Subcellular location	Nucleus By similarity.
Domain	The F-box is necessary for the interaction with ASK proteins By similarity.
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ASK11	O49484	3	EBI-591174,EBI-401185
SKP1A	Q39255	4	EBI-591174,EBI-532357
SKP1B	Q9FHW7	6	EBI-591174,EBI-604076

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	SKP1-interacting partner 15		PRO_0000273536
Regions
	Domain	3 – 48	46	F-box

Sequences

	Sequence		Length	Mass (Da)
	O49279 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 1D8584587C616173	FASTA	374	42,494
	10 20 30 40 50 60 MESSPVNCLP PDSLHQIFSS LPIRDIMICR SVCKFFNQLL TSQCFIEIIS TRPPLNLLAL 70 80 90 100 110 120 RPPHHHHSHR HSGNGHATNI RPYIHVYDPE QNQWFRFNLD FLPFRSPQPV ASSSGLIYLW 130 140 150 160 170 180 GDSIDLAESS KSLVACNPLT RQFKVLPQLG SAWSRHGTVL VDSVNRVMVL TELAALYYSG 190 200 210 220 230 240 TVVANQWLKF SSNLPSKPRS PVLMSSSVFA LCDVGSPWRS QWKLFSCKLT NLTITHTNWV 250 260 270 280 290 300 CLEKHEWGDI FDIIKRPRLL RGNGDSKLLM IGGLKSTFSL NPACSTILIL RLDLESLEWE 310 320 330 340 350 360 EAGRMPLEMY RGFQESSKFK VFGGGDRVYF SAKRMGKLAM WDCWQGWRWI EGVPGYADGL 370 CRGFVFDAKL TLMP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"F-box proteins in Arabidopsis." Xiao W., Jang J.-C. Trends Plant Sci. 5:454-457(2000) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[6]	"The F-box subunit of the SCF E3 complex is encoded by a diverse superfamily of genes in Arabidopsis." Gagne J.M., Downes B.P., Shiu S.-H., Durski A.M., Vierstra R.D. Proc. Natl. Acad. Sci. U.S.A. 99:11519-11524(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SKP1A/ASK1; SKP1B/ASK2; ASK11 AND ASK13.
[7]	"Protein interaction analysis of SCF ubiquitin E3 ligase subunits from Arabidopsis." Risseeuw E.P., Daskalchuk T.E., Banks T.W., Liu E., Cotelesage J., Hellmann H., Estelle M., Somers D.E., Crosby W.L. Plant J. 34:753-767(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SKPIB/ASK2.
+	Additional computationally mapped references.

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002291 Genomic DNA. Translation: AAC00632.1. CP002684 Genomic DNA. Translation: AEE35903.1. AY065102 mRNA. Translation: AAL38278.1. BT008455 mRNA. Translation: AAP37814.1. AY085473 mRNA. Translation: AAM62699.1.
IPI	IPI00535488.
PIR	A96798.
RefSeq	NP_565142.1. NM_106343.3.
UniGene	At.21630. At.67164.
3D structure databases
ProteinModelPortal	O49279.
SMR	O49279. Positions 6-44.
ModBase	Search...
Protein-protein interaction databases
IntAct	O49279. 12 interactions.
STRING	3702.AT1G76920.1-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G76920.1; AT1G76920.1; AT1G76920.
GeneID	844027.
KEGG	ath:AT1G76920.
Organism-specific databases
TAIR	At1g76920.
Phylogenomic databases
eggNOG	NOG324999.
HOGENOM	HOG000241138.
InParanoid	O49279.
OMA	VGSPWRS.
PhylomeDB	O49279.
ProtClustDB	CLSN2689248.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Genevestigator	O49279.
Family and domain databases
Gene3D	2.120.10.80. 2 hits.
InterPro	IPR001810. F-box_dom_cyclin-like. IPR015915. Kelch-typ_b-propeller. IPR011498. Kelch_2. [Graphical view]
Pfam	PF00646. F-box. 1 hit. PF07646. Kelch_2. 1 hit. [Graphical view]
SMART	SM00256. FBOX. 1 hit. [Graphical view]
SUPFAM	SSF81383. F-box_dom_Skp2-like. 1 hit.
PROSITE	PS50181. FBOX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SKI15_ARATH

Accession

Primary (citable) accession number: O49279

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program