Reviewed, UniProtKB/Swiss-Prot O49203 (NDK3_ARATH)

Names and origin

Protein names

Recommended name:
    Nucleoside diphosphate kinase III, chloroplastic/mitochondrial
      Short name=NDP kinase III
      Short name=NDPK III
      Short name=NDK III
    EC=2.7.4.6

Gene names

Name:	NDPK3
Ordered Locus Names:	At4g11010
ORF Names:	F8M12.12, F25I24.220

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Shows the highest specificity towards GDP By similarity.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Subunit structure	Homohexamer Probable.
Subcellular location	Plastid › chloroplast thylakoid lumen. Mitochondrion intermembrane space. Ref.1 Ref.5 Ref.6
Sequence similarities	Belongs to the NDK family.
Caution	Ref.1 demonstrates a thylakoid lumen location, while Ref.6 shows a mitochondrial location.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Chloroplast Mitochondrion Plastid Thylakoid
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro response to oxidative stress Inferred from direct assay. Source: TAIR
Cellular component	chloroplast thylakoid lumen Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial inner membrane Inferred from direct assay. Source: TAIR mitochondrial intermembrane space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 85	85	Chloroplast and mitochondrion Ref.5
	Chain	86 – 238	153	Nucleoside diphosphate kinase III, chloroplastic/mitochondrial		PRO_0000019435
Sites
	Active site	202	1	Pros-phosphohistidine intermediate By similarity
	Binding site	96	1	ATP By similarity
	Binding site	144	1	ATP By similarity
	Binding site	172	1	ATP By similarity
	Binding site	178	1	ATP By similarity
	Binding site	189	1	ATP By similarity
	Binding site	199	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O49203-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 6F05190005F7A1D9	FASTA	238	25,734
	10 20 30 40 50 60 MSSQICRSAS KAAKSLLSSA KNARFFSEGR AIGAAAAVSA SGKIPLYASN FARSSGSGVA 70 80 90 100 110 120 SKSWITGLLA LPAAAYMIQD QEVLAAEMER TFIAIKPDGV QRGLISEIIS RFERKGFKLV 130 140 150 160 170 180 GIKVIVPSKD FAQKHYHDLK ERPFFNGLCD FLSSGPVIAM VWEGDGVIRY GRKLIGATDP 190 200 210 220 230 QKSEPGTIRG DLAVTVGRNI IHGSDGPETA KDEISLWFKP QELVSYTSNS EKWLYGDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Multiple evidence for nucleotide metabolism in the chloroplast thylakoid lumen." Spetea C., Hundal T., Lundin B., Heddad M., Adamska I., Andersson B. Proc. Natl. Acad. Sci. U.S.A. 101:1409-1414(2004) [PubMed: 14736920] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, CHARACTERIZATION. Strain: cv. Columbia.
[2]	Lee J., Song P.-S., Hahn T.R., Choi G. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Proteomic approach to identify novel mitochondrial proteins in Arabidopsis." Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P. Plant Physiol. 127:1694-1710(2001) [PubMed: 11743114] [Abstract] Cited for: PROTEIN SEQUENCE OF 86-103 AND 122-135, SUBCELLULAR LOCATION. Tissue: Leaf and Stem.
[6]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF044265 mRNA. Translation: AAC00512.1. AF080118 Genomic DNA. Translation: AAC33956.1. AL049525 Genomic DNA. Translation: CAB40069.1. AL161518 Genomic DNA. Translation: CAB81202.1. AY062964 mRNA. Translation: AAL33810.1. AY035184 mRNA. Translation: AAK59688.1.
IPI	IPI00521242.
PIR	T01877.
RefSeq	NP_192839.1.
UniGene	At.47514
3D structure databases
HSSP	HSSP built from PDB template 1JXV based on UniProtKB P15531.
SMR	O49203. Positions 86-236.
ModBase	Search...
Protein-protein interaction databases
STRING	O49203.
Proteomic databases
PRIDE	O49203.
ProMEX	O49203.
Genome annotation databases
GeneID	826702.
GenomeReviews	Gene locus AT4G11010 in contig CT486007_GR.
KEGG	ath:AT4G11010.
NMPDR	fig|3702.1.peg.18766.
Organism-specific databases
GeneFarm	1971. 147.
TAIR	At4g11010.
Phylogenomic databases
OMA	PAAVYML.
Enzyme and pathway databases
BRENDA	2.7.4.6. 302.
Gene expression databases
ArrayExpress	O49203.
Genevestigator	O49203.
GermOnline	AT4G11010. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001564. Nuc_diP_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
ProDom	PD001018. NDK. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00562. NDK. 1 hit. [Graphical view]
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDK3_ARATH

Accession

Primary (citable) accession number: O49203

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	November 3, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)