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Protein

Nucleoside diphosphate kinase III, chloroplastic/mitochondrial

Gene

NDPK3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Shows the highest specificity towards GDP (By similarity).By similarity

Caution

PubMed:14736920 demonstrates a thylakoid lumen location, while PubMed:14671022 shows a mitochondrial location.Curated

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96ATPBy similarity1
Binding sitei144ATPBy similarity1
Binding sitei172ATPBy similarity1
Binding sitei178ATPBy similarity1
Binding sitei189ATPBy similarity1
Binding sitei199ATPBy similarity1
Active sitei202Pros-phosphohistidine intermediateBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cobalt ion binding Source: TAIR
  • nucleoside diphosphate kinase activity Source: GO_Central
  • zinc ion binding Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processNucleotide metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G11010-MONOMER
ReactomeiR-ATH-499943 Interconversion of nucleotide di- and triphosphates
R-ATH-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase III, chloroplastic/mitochondrial (EC:2.7.4.6)
Short name:
NDK III
Short name:
NDP kinase III
Short name:
NDPK III
Gene namesi
Name:NDPK3
Ordered Locus Names:At4g11010
ORF Names:F25I24.220, F8M12.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G11010
TAIRilocus:2123421 AT4G11010

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 85Chloroplast and mitochondrion1 PublicationAdd BLAST85
ChainiPRO_000001943586 – 238Nucleoside diphosphate kinase III, chloroplastic/mitochondrialAdd BLAST153

Proteomic databases

PaxDbiO49203
PRIDEiO49203

Expressioni

Gene expression databases

ExpressionAtlasiO49203 baseline and differential
GenevisibleiO49203 AT

Interactioni

Subunit structurei

Homohexamer.Curated

Protein-protein interaction databases

BioGridi12001, 1 interactor
STRINGi3702.AT4G11010.1

Structurei

3D structure databases

ProteinModelPortaliO49203
SMRiO49203
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NDK family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0888 Eukaryota
COG0105 LUCA
HOGENOMiHOG000224564
InParanoidiO49203
KOiK00940
OMAiLKDMPFY
OrthoDBiEOG09360LYS
PhylomeDBiO49203

Family and domain databases

Gene3Di3.30.70.141, 1 hit
HAMAPiMF_00451 NDP_kinase, 1 hit
InterProiView protein in InterPro
IPR034907 NDK-like_dom
IPR036850 NDK-like_dom_sf
IPR001564 Nucleoside_diP_kinase
IPR023005 Nucleoside_diP_kinase_AS
PfamiView protein in Pfam
PF00334 NDK, 1 hit
PRINTSiPR01243 NUCDPKINASE
SMARTiView protein in SMART
SM00562 NDK, 1 hit
SUPFAMiSSF54919 SSF54919, 1 hit
PROSITEiView protein in PROSITE
PS00469 NDP_KINASES, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O49203-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSQICRSAS KAAKSLLSSA KNARFFSEGR AIGAAAAVSA SGKIPLYASN
60 70 80 90 100
FARSSGSGVA SKSWITGLLA LPAAAYMIQD QEVLAAEMER TFIAIKPDGV
110 120 130 140 150
QRGLISEIIS RFERKGFKLV GIKVIVPSKD FAQKHYHDLK ERPFFNGLCD
160 170 180 190 200
FLSSGPVIAM VWEGDGVIRY GRKLIGATDP QKSEPGTIRG DLAVTVGRNI
210 220 230
IHGSDGPETA KDEISLWFKP QELVSYTSNS EKWLYGDN
Length:238
Mass (Da):25,734
Last modified:June 1, 1998 - v1
Checksum:i6F05190005F7A1D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044265 mRNA Translation: AAC00512.1
AF080118 Genomic DNA Translation: AAC33956.1
AL049525 Genomic DNA Translation: CAB40069.1
AL161518 Genomic DNA Translation: CAB81202.1
CP002687 Genomic DNA Translation: AEE82961.1
AY062964 mRNA Translation: AAL33810.1
AY035184 mRNA Translation: AAK59688.1
PIRiT01877
RefSeqiNP_192839.1, NM_117171.4
UniGeneiAt.47514

Genome annotation databases

EnsemblPlantsiAT4G11010.1; AT4G11010.1; AT4G11010
GeneIDi826702
GrameneiAT4G11010.1; AT4G11010.1; AT4G11010
KEGGiath:AT4G11010

Similar proteinsi

Entry informationi

Entry nameiNDK3_ARATH
AccessioniPrimary (citable) accession number: O49203
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: April 25, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health