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Protein

Nucleoside diphosphate kinase III, chloroplastic/mitochondrial

Gene

NDPK3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Shows the highest specificity towards GDP (By similarity).By similarity

Catalytic activityi

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei96ATPBy similarity1
Binding sitei144ATPBy similarity1
Binding sitei172ATPBy similarity1
Binding sitei178ATPBy similarity1
Binding sitei189ATPBy similarity1
Binding sitei199ATPBy similarity1
Active sitei202Pros-phosphohistidine intermediateBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cobalt ion binding Source: TAIR
  • nucleoside diphosphate kinase activity Source: GO_Central
  • zinc ion binding Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G11010-MONOMER.
ReactomeiR-ATH-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
R-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate kinase III, chloroplastic/mitochondrial (EC:2.7.4.6)
Short name:
NDK III
Short name:
NDP kinase III
Short name:
NDPK III
Gene namesi
Name:NDPK3
Ordered Locus Names:At4g11010
ORF Names:F25I24.220, F8M12.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G11010.

Subcellular locationi

GO - Cellular componenti

  • chloroplast thylakoid lumen Source: UniProtKB-SubCell
  • mitochondrial inner membrane Source: TAIR
  • mitochondrial intermembrane space Source: UniProtKB-SubCell
  • mitochondrion Source: TAIR
  • plastid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 85Chloroplast and mitochondrion1 PublicationAdd BLAST85
ChainiPRO_000001943586 – 238Nucleoside diphosphate kinase III, chloroplastic/mitochondrialAdd BLAST153

Proteomic databases

PaxDbiO49203.
PRIDEiO49203.

Expressioni

Gene expression databases

GenevisibleiO49203. AT.

Interactioni

Subunit structurei

Homohexamer.Curated

Protein-protein interaction databases

BioGridi12001. 1 interactor.
STRINGi3702.AT4G11010.1.

Structurei

3D structure databases

ProteinModelPortaliO49203.
SMRiO49203.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NDK family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
HOGENOMiHOG000224564.
InParanoidiO49203.
KOiK00940.
OMAiWFEDKEL.
OrthoDBiEOG09360LYS.
PhylomeDBiO49203.

Family and domain databases

HAMAPiMF_00451. NDP_kinase. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O49203-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSQICRSAS KAAKSLLSSA KNARFFSEGR AIGAAAAVSA SGKIPLYASN
60 70 80 90 100
FARSSGSGVA SKSWITGLLA LPAAAYMIQD QEVLAAEMER TFIAIKPDGV
110 120 130 140 150
QRGLISEIIS RFERKGFKLV GIKVIVPSKD FAQKHYHDLK ERPFFNGLCD
160 170 180 190 200
FLSSGPVIAM VWEGDGVIRY GRKLIGATDP QKSEPGTIRG DLAVTVGRNI
210 220 230
IHGSDGPETA KDEISLWFKP QELVSYTSNS EKWLYGDN
Length:238
Mass (Da):25,734
Last modified:June 1, 1998 - v1
Checksum:i6F05190005F7A1D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044265 mRNA. Translation: AAC00512.1.
AF080118 Genomic DNA. Translation: AAC33956.1.
AL049525 Genomic DNA. Translation: CAB40069.1.
AL161518 Genomic DNA. Translation: CAB81202.1.
CP002687 Genomic DNA. Translation: AEE82961.1.
AY062964 mRNA. Translation: AAL33810.1.
AY035184 mRNA. Translation: AAK59688.1.
PIRiT01877.
RefSeqiNP_192839.1. NM_117171.4.
UniGeneiAt.47514.

Genome annotation databases

EnsemblPlantsiAT4G11010.1; AT4G11010.1; AT4G11010.
GeneIDi826702.
GrameneiAT4G11010.1; AT4G11010.1; AT4G11010.
KEGGiath:AT4G11010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044265 mRNA. Translation: AAC00512.1.
AF080118 Genomic DNA. Translation: AAC33956.1.
AL049525 Genomic DNA. Translation: CAB40069.1.
AL161518 Genomic DNA. Translation: CAB81202.1.
CP002687 Genomic DNA. Translation: AEE82961.1.
AY062964 mRNA. Translation: AAL33810.1.
AY035184 mRNA. Translation: AAK59688.1.
PIRiT01877.
RefSeqiNP_192839.1. NM_117171.4.
UniGeneiAt.47514.

3D structure databases

ProteinModelPortaliO49203.
SMRiO49203.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12001. 1 interactor.
STRINGi3702.AT4G11010.1.

Proteomic databases

PaxDbiO49203.
PRIDEiO49203.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G11010.1; AT4G11010.1; AT4G11010.
GeneIDi826702.
GrameneiAT4G11010.1; AT4G11010.1; AT4G11010.
KEGGiath:AT4G11010.

Organism-specific databases

TAIRiAT4G11010.

Phylogenomic databases

eggNOGiKOG0888. Eukaryota.
COG0105. LUCA.
HOGENOMiHOG000224564.
InParanoidiO49203.
KOiK00940.
OMAiWFEDKEL.
OrthoDBiEOG09360LYS.
PhylomeDBiO49203.

Enzyme and pathway databases

BioCyciARA:AT4G11010-MONOMER.
ReactomeiR-ATH-499943. Synthesis and interconversion of nucleotide di- and triphosphates.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiO49203.

Gene expression databases

GenevisibleiO49203. AT.

Family and domain databases

HAMAPiMF_00451. NDP_kinase. 1 hit.
InterProiIPR001564. Nucleoside_diP_kinase.
IPR023005. Nucleoside_diP_kinase_AS.
[Graphical view]
PfamiPF00334. NDK. 1 hit.
[Graphical view]
PRINTSiPR01243. NUCDPKINASE.
SMARTiSM00562. NDK. 1 hit.
[Graphical view]
PROSITEiPS00469. NDP_KINASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDK3_ARATH
AccessioniPrimary (citable) accession number: O49203
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

PubMed:14736920 demonstrates a thylakoid lumen location, while PubMed:14671022 shows a mitochondrial location.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.