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O49139

- CMT1_ARATH

UniProt

O49139 - CMT1_ARATH

Protein

Putative DNA (cytosine-5)-methyltransferase CMT1

Gene

CMT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Protein uncertaini
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (25 Jul 2006)
      Previous versions | rss
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    Functioni

    May be involved in the CpXpG methylation and in gene silencing.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei417 – 4171PROSITE-ProRule annotation

    GO - Molecular functioni

    1. chromatin binding Source: InterPro
    2. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    3. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. chromatin modification Source: UniProtKB-KW
    2. DNA mediated transformation Source: TAIR
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Methyltransferase, Transferase

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciARA:AT1G80740-MONOMER.

    Protein family/group databases

    REBASEi3261. M.AthCMT1P.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative DNA (cytosine-5)-methyltransferase CMT1 (EC:2.1.1.37)
    Alternative name(s):
    Chromomethylase 1
    Protein CHROMOMETHYLASE 1
    Gene namesi
    Name:CMT1
    Ordered Locus Names:At1g80740
    ORF Names:F23A5.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G80740.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 791791Putative DNA (cytosine-5)-methyltransferase CMT1PRO_0000246691Add
    BLAST

    Proteomic databases

    PaxDbiO49139.
    PRIDEiO49139.

    Expressioni

    Tissue specificityi

    Expressed in flowers. Not detected in leaves, roots, seedlings and plants prior formation of flower buds.1 Publication

    Gene expression databases

    GenevestigatoriO49139.

    Structurei

    3D structure databases

    ProteinModelPortaliO49139.
    SMRiO49139. Positions 12-778.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini79 – 199121BAHPROSITE-ProRule annotationAdd
    BLAST
    Domaini225 – 768544SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini339 – 40466ChromoPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili308 – 33326Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 BAH domain.PROSITE-ProRule annotation
    Contains 1 chromo domain.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0270.
    InParanoidiO49139.
    KOiK00558.
    OMAiFGMASQG.
    PhylomeDBiO49139.

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR001025. BAH_dom.
    IPR001525. C5_MeTfrase.
    IPR025821. C5_MeTfrase_pln.
    IPR017984. Chromo_dom_subgr.
    IPR023780. Chromo_domain.
    IPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR023779. Chromodomain_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF01426. BAH. 1 hit.
    PF00385. Chromo. 1 hit.
    PF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    PR00504. CHROMODOMAIN.
    SMARTiSM00439. BAH. 1 hit.
    SM00298. CHROMO. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 4 hits.
    SSF54160. SSF54160. 1 hit.
    PROSITEiPS51038. BAH. 1 hit.
    PS00598. CHROMO_1. 1 hit.
    PS50013. CHROMO_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O49139-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAARNKQKKR AEPESDLCFA GKPMSVVEST IRWPHRYQSK KTKLQAPTKK    50
    PANKGGKKED EEIIKQAKCH FDKALVDGVL INLNDDVYVT GLPGKLKFIA 100
    KVIELFEADD GVPYCRFRWY YRPEDTLIER FSHLVQPKRV FLSNDENDNP 150
    LTCIWSKVNI AKVPLPKITS RIEQRVIPPC DYYYDMKYEV PYLNFTSADD 200
    GSDASSSLSS DSALNCFENL HKDEKFLLDL YSGCGAMSTG FCMGASISGV 250
    KLITKWSVDI NKFACDSLKL NHPETEVRNE AAEDFLALLK EWKRLCEKFS 300
    LVSSTEPVES ISELEDEEVE ENDDIDEAST GAELEPGEFE VEKFLGIMFG 350
    DPQGTGEKTL QLMVRWKGYN SSYDTWEPYS GLGNCKEKLK EYVIDGFKSH 400
    LLPLPGTVYT VCGGPPCQGI SGYNRYRNNE APLEDQKNQQ LLVFLDIIDF 450
    LKPNYVLMEN VVDLLRFSKG FLARHAVASF VAMNYQTRLG MMAAGSYGLP 500
    QLRNRVFLWA AQPSEKLPPY PLPTHEVAKK FNTPKEFKDL QVGRIQMEFL 550
    KLDNALTLAD AISDLPPVTN YVANDVMDYN DAAPKTEFEN FISLKRSETL 600
    LPAFGGDPTR RLFDHQPLVL GDDDLERVSY IPKQKGANYR DMPGVLVHNN 650
    KAEINPRFRA KLKSGKNVVP AYAISFIKGK SKKPFGRLWG DEIVNTVVTR 700
    AEPHNQCVIH PMQNRVLSVR ENARLQGFPD CYKLCGTIKE KYIQVGNAVA 750
    VPVGVALGYA FGMASQGLTD DEPVIKLPFK YPECMQAKDQ I 791
    Length:791
    Mass (Da):89,219
    Last modified:July 25, 2006 - v2
    Checksum:iA5ECFDBC274B215C
    GO
    Isoform 2 (identifier: O49139-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         568-570: VTN → CCR
         571-791: Missing.

    Note: Alternative splice site used 50% of the time in cv. Columbia.

    Show »
    Length:570
    Mass (Da):64,557
    Checksum:iD08B184B4F3C6ADA
    GO

    Sequence cautioni

    The sequence AAA98912.1 differs from that shown. Reason: Erroneous gene model prediction.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti148 – 1481D → G in strain: cv. Metz-0.
    Natural varianti196 – 1961T → I in strain: cv. Kl-0.
    Natural varianti198 – 1981A → T in strain: cv. No-0.
    Natural varianti249 – 791543Missing in strain: cv. Metz-0.
    Add
    BLAST
    Natural varianti560 – 5601D → V in strain: cv. Landsberg erecta, cv. No-0 and cv. RLD.
    Natural varianti561 – 791231Missing in strain: cv. Landsberg erecta, cv. No-0 and cv. RLD.
    Add
    BLAST
    Natural varianti604 – 6041F → C in strain: cv. Nd-1, Nd-0 and cv. Kl-0.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei568 – 5703VTN → CCR in isoform 2. 1 PublicationVSP_019857
    Alternative sequencei571 – 791221Missing in isoform 2. 1 PublicationVSP_019858Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF039364 mRNA. Translation: AAB95485.1.
    AF039366 Genomic DNA. Translation: AAC02659.1.
    AF039367 Genomic DNA. Translation: AAC02660.1.
    AF039368 Genomic DNA. Translation: AAC02661.1.
    AF039369 Genomic DNA. Translation: AAC02662.1.
    AF039370 Genomic DNA. Translation: AAC02663.1.
    AF039371 Genomic DNA. Translation: AAC02665.1.
    AF039372 Genomic DNA. Translation: AAC02667.1.
    AF039373 Genomic DNA. Translation: AAC02668.1.
    U53501 Genomic DNA. Translation: AAA98912.1. Sequence problems.
    AC011713 Genomic DNA. Translation: AAF14662.1.
    CP002684 Genomic DNA. Translation: AEE36442.1.
    PIRiH96839.
    RefSeqiNP_565245.1. NM_106722.2. [O49139-1]
    UniGeneiAt.5460.

    Genome annotation databases

    EnsemblPlantsiAT1G80740.1; AT1G80740.1; AT1G80740. [O49139-1]
    GeneIDi844413.
    KEGGiath:AT1G80740.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF039364 mRNA. Translation: AAB95485.1 .
    AF039366 Genomic DNA. Translation: AAC02659.1 .
    AF039367 Genomic DNA. Translation: AAC02660.1 .
    AF039368 Genomic DNA. Translation: AAC02661.1 .
    AF039369 Genomic DNA. Translation: AAC02662.1 .
    AF039370 Genomic DNA. Translation: AAC02663.1 .
    AF039371 Genomic DNA. Translation: AAC02665.1 .
    AF039372 Genomic DNA. Translation: AAC02667.1 .
    AF039373 Genomic DNA. Translation: AAC02668.1 .
    U53501 Genomic DNA. Translation: AAA98912.1 . Sequence problems.
    AC011713 Genomic DNA. Translation: AAF14662.1 .
    CP002684 Genomic DNA. Translation: AEE36442.1 .
    PIRi H96839.
    RefSeqi NP_565245.1. NM_106722.2. [O49139-1 ]
    UniGenei At.5460.

    3D structure databases

    ProteinModelPortali O49139.
    SMRi O49139. Positions 12-778.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3261. M.AthCMT1P.

    Proteomic databases

    PaxDbi O49139.
    PRIDEi O49139.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G80740.1 ; AT1G80740.1 ; AT1G80740 . [O49139-1 ]
    GeneIDi 844413.
    KEGGi ath:AT1G80740.

    Organism-specific databases

    TAIRi AT1G80740.

    Phylogenomic databases

    eggNOGi COG0270.
    InParanoidi O49139.
    KOi K00558.
    OMAi FGMASQG.
    PhylomeDBi O49139.

    Enzyme and pathway databases

    BioCyci ARA:AT1G80740-MONOMER.

    Gene expression databases

    Genevestigatori O49139.

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR001025. BAH_dom.
    IPR001525. C5_MeTfrase.
    IPR025821. C5_MeTfrase_pln.
    IPR017984. Chromo_dom_subgr.
    IPR023780. Chromo_domain.
    IPR000953. Chromo_domain/shadow.
    IPR016197. Chromodomain-like.
    IPR023779. Chromodomain_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF01426. BAH. 1 hit.
    PF00385. Chromo. 1 hit.
    PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    PR00504. CHROMODOMAIN.
    SMARTi SM00439. BAH. 1 hit.
    SM00298. CHROMO. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 4 hits.
    SSF54160. SSF54160. 1 hit.
    PROSITEi PS51038. BAH. 1 hit.
    PS00598. CHROMO_1. 1 hit.
    PS50013. CHROMO_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A DNA methyltransferase homolog with a chromodomain exists in multiple polymorphic forms in Arabidopsis."
      Henikoff S., Comai L.
      Genetics 149:307-318(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
      Strain: cv. Columbia, cv. Kl-0, cv. Landsberg erecta, cv. Metz-0, cv. Nd-0, cv. Nd-1 and cv. RLD.
    2. "A 37.5 Kb sequence from Arabidopsis thaliana chromosome I."
      Goodman H.M., Gallant P., Keifer-Higgins S., Rubenfield M., Church G.M.
      Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiCMT1_ARATH
    AccessioniPrimary (citable) accession number: O49139
    Secondary accession number(s): O49137
    , O49138, O49141, O50057, O50073, Q38940, Q7G196
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3