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Protein

Putative DNA (cytosine-5)-methyltransferase CMT1

Gene

CMT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Protein uncertaini

Functioni

May be involved in the CpXpG methylation and in gene silencing.By similarity

Caution

Could be the product of a pseudogene. The protein is severely truncated in several ecotypes and the gene even harbors a complete retrotransposon in 3 ecotypes.Curated

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei417PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • chromatin organization Source: UniProtKB-KW
  • DNA mediated transformation Source: TAIR
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Methyltransferase, Transferase
Biological processTranscription, Transcription regulation
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3262 M.AarAIP

Names & Taxonomyi

Protein namesi
Recommended name:
Putative DNA (cytosine-5)-methyltransferase CMT1 (EC:2.1.1.37)
Alternative name(s):
Chromomethylase 1
Protein CHROMOMETHYLASE 1
Gene namesi
Name:CMT1
Ordered Locus Names:At1g80740
ORF Names:F23A5.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G80740
TAIRilocus:2025787 AT1G80740

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002466911 – 791Putative DNA (cytosine-5)-methyltransferase CMT1Add BLAST791

Proteomic databases

PaxDbiO49139
PRIDEiO49139

Expressioni

Tissue specificityi

Expressed in flowers. Not detected in leaves, roots, seedlings and plants prior formation of flower buds.1 Publication

Gene expression databases

ExpressionAtlasiO49139 baseline and differential
GenevisibleiO49139 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G80740.1

Structurei

3D structure databases

ProteinModelPortaliO49139
SMRiO49139
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini79 – 199BAHPROSITE-ProRule annotationAdd BLAST121
Domaini225 – 768SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST544
Domaini339 – 404ChromoPROSITE-ProRule annotationAdd BLAST66

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili308 – 333Sequence analysisAdd BLAST26

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IH5K Eukaryota
COG0270 LUCA
InParanoidiO49139
KOiK00558
OMAiSMNYQAR
OrthoDBiEOG093605JX
PhylomeDBiO49139

Family and domain databases

CDDicd00024 CHROMO, 1 hit
InterProiView protein in InterPro
IPR001025 BAH_dom
IPR001525 C5_MeTfrase
IPR016197 Chromo-like_dom_sf
IPR000953 Chromo/chromo_shadow_dom
IPR017984 Chromo_dom_subgr
IPR023780 Chromo_domain
IPR023779 Chromodomain_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01426 BAH, 1 hit
PF00385 Chromo, 1 hit
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
PR00504 CHROMODOMAIN
SMARTiView protein in SMART
SM00439 BAH, 1 hit
SM00298 CHROMO, 1 hit
SUPFAMiSSF53335 SSF53335, 4 hits
SSF54160 SSF54160, 1 hit
PROSITEiView protein in PROSITE
PS51038 BAH, 1 hit
PS00598 CHROMO_1, 1 hit
PS50013 CHROMO_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O49139-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAARNKQKKR AEPESDLCFA GKPMSVVEST IRWPHRYQSK KTKLQAPTKK
60 70 80 90 100
PANKGGKKED EEIIKQAKCH FDKALVDGVL INLNDDVYVT GLPGKLKFIA
110 120 130 140 150
KVIELFEADD GVPYCRFRWY YRPEDTLIER FSHLVQPKRV FLSNDENDNP
160 170 180 190 200
LTCIWSKVNI AKVPLPKITS RIEQRVIPPC DYYYDMKYEV PYLNFTSADD
210 220 230 240 250
GSDASSSLSS DSALNCFENL HKDEKFLLDL YSGCGAMSTG FCMGASISGV
260 270 280 290 300
KLITKWSVDI NKFACDSLKL NHPETEVRNE AAEDFLALLK EWKRLCEKFS
310 320 330 340 350
LVSSTEPVES ISELEDEEVE ENDDIDEAST GAELEPGEFE VEKFLGIMFG
360 370 380 390 400
DPQGTGEKTL QLMVRWKGYN SSYDTWEPYS GLGNCKEKLK EYVIDGFKSH
410 420 430 440 450
LLPLPGTVYT VCGGPPCQGI SGYNRYRNNE APLEDQKNQQ LLVFLDIIDF
460 470 480 490 500
LKPNYVLMEN VVDLLRFSKG FLARHAVASF VAMNYQTRLG MMAAGSYGLP
510 520 530 540 550
QLRNRVFLWA AQPSEKLPPY PLPTHEVAKK FNTPKEFKDL QVGRIQMEFL
560 570 580 590 600
KLDNALTLAD AISDLPPVTN YVANDVMDYN DAAPKTEFEN FISLKRSETL
610 620 630 640 650
LPAFGGDPTR RLFDHQPLVL GDDDLERVSY IPKQKGANYR DMPGVLVHNN
660 670 680 690 700
KAEINPRFRA KLKSGKNVVP AYAISFIKGK SKKPFGRLWG DEIVNTVVTR
710 720 730 740 750
AEPHNQCVIH PMQNRVLSVR ENARLQGFPD CYKLCGTIKE KYIQVGNAVA
760 770 780 790
VPVGVALGYA FGMASQGLTD DEPVIKLPFK YPECMQAKDQ I
Length:791
Mass (Da):89,219
Last modified:July 25, 2006 - v2
Checksum:iA5ECFDBC274B215C
GO
Isoform 2 (identifier: O49139-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     568-570: VTN → CCR
     571-791: Missing.

Note: Alternative splice site used 50% of the time in cv. Columbia.
Show »
Length:570
Mass (Da):64,557
Checksum:iD08B184B4F3C6ADA
GO

Sequence cautioni

The sequence AAA98912 differs from that shown. Reason: Erroneous gene model prediction.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti148D → G in strain: cv. Metz-0. 1
Natural varianti196T → I in strain: cv. Kl-0. 1
Natural varianti198A → T in strain: cv. No-0. 1
Natural varianti249 – 791Missing in strain: cv. Metz-0. Add BLAST543
Natural varianti560D → V in strain: cv. Landsberg erecta, cv. No-0 and cv. RLD. 1
Natural varianti561 – 791Missing in strain: cv. Landsberg erecta, cv. No-0 and cv. RLD. Add BLAST231
Natural varianti604F → C in strain: cv. Nd-1, Nd-0 and cv. Kl-0. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_019857568 – 570VTN → CCR in isoform 2. 1 Publication3
Alternative sequenceiVSP_019858571 – 791Missing in isoform 2. 1 PublicationAdd BLAST221

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039364 mRNA Translation: AAB95485.1
AF039366 Genomic DNA Translation: AAC02659.1
AF039367 Genomic DNA Translation: AAC02660.1
AF039368 Genomic DNA Translation: AAC02661.1
AF039369 Genomic DNA Translation: AAC02662.1
AF039370 Genomic DNA Translation: AAC02663.1
AF039371 Genomic DNA Translation: AAC02665.1
AF039372 Genomic DNA Translation: AAC02667.1
AF039373 Genomic DNA Translation: AAC02668.1
U53501 Genomic DNA Translation: AAA98912.1 Sequence problems.
AC011713 Genomic DNA Translation: AAF14662.1
CP002684 Genomic DNA Translation: AEE36442.1
PIRiH96839
RefSeqiNP_565245.1, NM_106722.2 [O49139-1]
UniGeneiAt.5460

Genome annotation databases

EnsemblPlantsiAT1G80740.1; AT1G80740.1; AT1G80740 [O49139-1]
GeneIDi844413
GrameneiAT1G80740.1; AT1G80740.1; AT1G80740 [O49139-1]
KEGGiath:AT1G80740

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCMT1_ARATH
AccessioniPrimary (citable) accession number: O49139
Secondary accession number(s): O49137
, O49138, O49141, O50057, O50073, Q38940, Q7G196
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: July 25, 2006
Last modified: May 23, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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