Reviewed, UniProtKB/Swiss-Prot O48962 (EBP_ARATH)

Names and origin

Protein names

Recommended name:
    Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
    EC=5.3.3.5
Alternative name(s):
    Cholestenol Delta-isomerase
    Delta(8)-Delta(7) sterol isomerase
      Short name=D8-D7 sterol isomerase

Gene names

Ordered Locus Names:	At1g20050
ORF Names:	T20H2.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of Delta(8)-sterols to their corresponding Delta(7)-isomers By similarity.
Catalytic activity	5-alpha-cholest-7-en-3-beta-ol = 5-alpha-cholest-8-en-3-beta-ol.
Pathway	Steroid biosynthesis; sterol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the EBP family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sterol biosynthetic process Traceable author statement. Source: TAIR
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	C-8 sterol isomerase activity Ref.1 Inferred from mutant phenotype. Source: TAIR cholestenol delta-isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 223	223	Probable 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase		PRO_0000174345
Regions
	Transmembrane	28 – 48	21	Potential
	Transmembrane	58 – 78	21	Potential
	Transmembrane	115 – 135	21	Potential
	Transmembrane	175 – 195	21	Potential
Experimental info
	Sequence conflict	157	1	L → V in AAD04752. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O48962-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 8060845768EEBACC	FASTA	223	25,146
	10 20 30 40 50 60 MEELAHPYVP RDLNLPGYVP ISMSMSSIVS IYLGSSLLVV SLVWLLFGRK KAKLDKLLMC 70 80 90 100 110 120 WWTFTGLTHV ILEGYFVFSP EFFKDNTSAY LAEVWKEYSK GDSRYVGRDS AVVSVEGITA 130 140 150 160 170 180 VIVGPASLLA IYAIAKEKSY SYVLQLAISV CQLYGCLVYF ITAILEGDNF ATNSFYYYSY 190 200 210 220 YIGANCWWVL IPSLISFRCW KKICAAAAIA NNNVETKTKK KTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of an Arabidopsis thaliana C-8,7 sterol isomerase: functional and structural similarities to mammalian C-8,7 sterol isomerase/emopamil-binding protein." Grebenok R.J., Ohnmeiss T.E., Yamamoto A., Huntley E.D., Galbraith D.W., Della Penna D. Plant Mol. Biol. 38:807-815(1998) [PubMed: 9862498] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Benveniste P. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF030357 mRNA. Translation: AAD03489.1. U81498 mRNA. Translation: AAD04752.1. AC022472 Genomic DNA. Translation: AAF79909.1. AF334733 mRNA. Translation: AAG50111.1.
IPI	IPI00543529.
PIR	T51727.
RefSeq	NP_173433.1.
UniGene	At.10970 At.71675
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O48962.
Proteomic databases
PRIDE	O48962.
Genome annotation databases
GeneID	838593.
GenomeReviews	Gene locus AT1G20050 in contig CT485782_GR.
KEGG	ath:AT1G20050.
Organism-specific databases
TAIR	At1g20050.
Phylogenomic databases
OMA	LEGYFVF.
Enzyme and pathway databases
BioCyc	MetaCyc:AT1G20050-MON.
BRENDA	5.3.3.5. 302.
Gene expression databases
ArrayExpress	O48962.
Genevestigator	O48962.
GermOnline	AT1G20050. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR007905. EBP. [Graphical view]
PANTHER	PTHR14207. EBP. 1 hit.
Pfam	PF05241. EBP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EBP_ARATH

Accession

Primary (citable) accession number: O48962
Secondary accession number(s): Q9SAQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	June 1, 1998
Last modified:	November 3, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)