O48958 (C71E1_SORBI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxyphenylacetaldehyde oxime monooxygenase
EC=1.14.13.68

Alternative name(s):
Cytochrome P450 71E1

Gene names

Name:

CYP71E1

Organism

Sorghum bicolor (Sorghum) (Sorghum vulgare)

Taxonomic identifier

4558 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Sorghum

Protein attributes

Sequence length	531 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the conversion of p-hydroxyphenylacetaldoxime to p-hydroxymandelonitrile. The dehydration of the oxime to the corresponding nitrile is followed by a C-hydroxylation of the nitrile to produce p-hydroxymandelonitrile.
Catalytic activity	(Z)-4-hydroxyphenylacetaldehyde oxime + NADPH + O2 = (S)-4-hydroxymandelonitrile + NADP+ + 2 H2O.
Cofactor	Heme group By similarity.
Pathway	Secondary metabolite biosynthesis; dhurrin biosynthesis; dhurrin from L-tyrosine: step 2/3.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological_process	dhurrin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	4-hydroxyphenylacetaldehyde oxime monooxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro hydroxyphenylacetonitrile 2-monooxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 531	531	4-hydroxyphenylacetaldehyde oxime monooxygenase		PRO_0000052123
Regions
	Transmembrane	18 – 38	21	Helical; Potential
Sites
	Metal binding	468	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O48958 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 2272E4AA910890D2	FASTA	531	59,088
	10 20 30 40 50 60 MATTATPQLL GGSVPQQWQT CLLVLLPVLL VSYYLLTSRS RNRSRSGKLG GAPRLPPGPA 70 80 90 100 110 120 QLPILGNLHL LGPLPHKNLR ELARRYGPVM QLRLGTVPTV VVSSAEAARE VLKVHDVDCC 130 140 150 160 170 180 SRPASPGPKR LSYDLKNVGF APYGEYWREM RKLFALELLS MRRVKAACYA REQEMDRLVA 190 200 210 220 230 240 DLDRAAASKA SIVLNDHVFA LTDGIIGTVA FGNIYASKQF AHKERFQHVL DDAMDMMASF 250 260 270 280 290 300 SAEDFFPNAA GRLADRLSGF LARRERIFNE LDVFFEKVID QHMDPARPVP DNGGDLVDVL 310 320 330 340 350 360 INLCKEHDGT LRFTRDHVKA IVLDTFIGAI DTSSVTILWA MSELMRKPQV LRKAQAEVRA 370 380 390 400 410 420 AVGDDKPRVN SEDAAKIPYL KMVVKETLRL HPPATLLVPR ETMRDTTICG YDVPANTRVF 430 440 450 460 470 480 VNAWAIGRDP ASWPAPDEFN PDRFVGSDVD YYGSHFELIP FGAGRRICPG LTMGETNVTF 490 500 510 520 530 TLANLLYCYD WALPGAMKPE DVSMEETGAL TFHRKTPLVV VPTKYKNRRA A

References

[1]

"Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome P450 in the biosynthesis of the cyanogenic glucoside dhurrin." Bak S., Kahn R.A., Nielsen H.L., Moeller B.L., Halkier B.A. Plant Mol. Biol. 36:393-405(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. SS1000. Tissue: Etiolated seedling.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF029858 mRNA. Translation: AAC39318.1.
PIR	T14640.
3D structure databases
ProteinModelPortal	O48958.
ModBase	Search...
Protein-protein interaction databases
STRING	4558.Sb01g001180.1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	O48958.
Phylogenomic databases
eggNOG	COG2124.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-522.
UniPathway	UPA00757; UER00745.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C71E1_SORBI

Accession

Primary (citable) accession number: O48958

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program