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O48958 (C71E1_SORBI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxyphenylacetaldehyde oxime monooxygenase

EC=1.14.13.68
Alternative name(s):
Cytochrome P450 71E1
Gene names
Name:CYP71E1
OrganismSorghum bicolor (Sorghum) (Sorghum vulgare)
Taxonomic identifier4558 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeSorghum

Protein attributes

Sequence length531 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the conversion of p-hydroxyphenylacetaldoxime to p-hydroxymandelonitrile. The dehydration of the oxime to the corresponding nitrile is followed by a C-hydroxylation of the nitrile to produce p-hydroxymandelonitrile.

Catalytic activity

(Z)-4-hydroxyphenylacetaldehyde oxime + NADPH + O2 = (S)-4-hydroxymandelonitrile + NADP+ + 2 H2O.

Cofactor

Heme group By similarity.

Pathway

Secondary metabolite biosynthesis; dhurrin biosynthesis; dhurrin from L-tyrosine: step 2/3.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5315314-hydroxyphenylacetaldehyde oxime monooxygenase
PRO_0000052123

Regions

Transmembrane18 – 3821Helical; Potential

Sites

Metal binding4681Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
O48958 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 2272E4AA910890D2

FASTA53159,088
        10         20         30         40         50         60 
MATTATPQLL GGSVPQQWQT CLLVLLPVLL VSYYLLTSRS RNRSRSGKLG GAPRLPPGPA 

        70         80         90        100        110        120 
QLPILGNLHL LGPLPHKNLR ELARRYGPVM QLRLGTVPTV VVSSAEAARE VLKVHDVDCC 

       130        140        150        160        170        180 
SRPASPGPKR LSYDLKNVGF APYGEYWREM RKLFALELLS MRRVKAACYA REQEMDRLVA 

       190        200        210        220        230        240 
DLDRAAASKA SIVLNDHVFA LTDGIIGTVA FGNIYASKQF AHKERFQHVL DDAMDMMASF 

       250        260        270        280        290        300 
SAEDFFPNAA GRLADRLSGF LARRERIFNE LDVFFEKVID QHMDPARPVP DNGGDLVDVL 

       310        320        330        340        350        360 
INLCKEHDGT LRFTRDHVKA IVLDTFIGAI DTSSVTILWA MSELMRKPQV LRKAQAEVRA 

       370        380        390        400        410        420 
AVGDDKPRVN SEDAAKIPYL KMVVKETLRL HPPATLLVPR ETMRDTTICG YDVPANTRVF 

       430        440        450        460        470        480 
VNAWAIGRDP ASWPAPDEFN PDRFVGSDVD YYGSHFELIP FGAGRRICPG LTMGETNVTF 

       490        500        510        520        530 
TLANLLYCYD WALPGAMKPE DVSMEETGAL TFHRKTPLVV VPTKYKNRRA A 

« Hide

References

[1]"Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome P450 in the biosynthesis of the cyanogenic glucoside dhurrin."
Bak S., Kahn R.A., Nielsen H.L., Moeller B.L., Halkier B.A.
Plant Mol. Biol. 36:393-405(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. SS1000.
Tissue: Etiolated seedling.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF029858 mRNA. Translation: AAC39318.1.
PIRT14640.

3D structure databases

ProteinModelPortalO48958.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4558.Sb01g001180.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneO48958.

Phylogenomic databases

eggNOGCOG2124.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-522.
SABIO-RKO48958.
UniPathwayUPA00757; UER00745.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC71E1_SORBI
AccessionPrimary (citable) accession number: O48958
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: February 19, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways