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Protein

4-hydroxyphenylacetaldehyde oxime monooxygenase

Gene

CYP71E1

Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of p-hydroxyphenylacetaldoxime to p-hydroxymandelonitrile. The dehydration of the oxime to the corresponding nitrile is followed by a C-hydroxylation of the nitrile to produce p-hydroxymandelonitrile.

Catalytic activityi

(Z)-4-hydroxyphenylacetaldehyde oxime + NADPH + O2 = (S)-4-hydroxymandelonitrile + NADP+ + 2 H2O.

Cofactori

hemeBy similarity

Pathway: dhurrin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes dhurrin from L-tyrosine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Tyrosine N-monooxygenase (CYP79A1)
  2. 4-hydroxyphenylacetaldehyde oxime monooxygenase (CYP71E1)
  3. Cyanohydrin beta-glucosyltransferase (UGT85B1)
This subpathway is part of the pathway dhurrin biosynthesis, which is itself part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes dhurrin from L-tyrosine, the pathway dhurrin biosynthesis and in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi468 – 4681Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-522.
SABIO-RKO48958.
UniPathwayiUPA00757; UER00745.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxyphenylacetaldehyde oxime monooxygenase (EC:1.14.13.68)
Alternative name(s):
Cytochrome P450 71E1
Gene namesi
Name:CYP71E1
OrganismiSorghum bicolor (Sorghum) (Sorghum vulgare)
Taxonomic identifieri4558 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeSorghum

Organism-specific databases

GrameneiO48958.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei18 – 3821HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5315314-hydroxyphenylacetaldehyde oxime monooxygenasePRO_0000052123Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi4558.Sb01g001180.1.

Structurei

3D structure databases

ProteinModelPortaliO48958.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O48958-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATTATPQLL GGSVPQQWQT CLLVLLPVLL VSYYLLTSRS RNRSRSGKLG
60 70 80 90 100
GAPRLPPGPA QLPILGNLHL LGPLPHKNLR ELARRYGPVM QLRLGTVPTV
110 120 130 140 150
VVSSAEAARE VLKVHDVDCC SRPASPGPKR LSYDLKNVGF APYGEYWREM
160 170 180 190 200
RKLFALELLS MRRVKAACYA REQEMDRLVA DLDRAAASKA SIVLNDHVFA
210 220 230 240 250
LTDGIIGTVA FGNIYASKQF AHKERFQHVL DDAMDMMASF SAEDFFPNAA
260 270 280 290 300
GRLADRLSGF LARRERIFNE LDVFFEKVID QHMDPARPVP DNGGDLVDVL
310 320 330 340 350
INLCKEHDGT LRFTRDHVKA IVLDTFIGAI DTSSVTILWA MSELMRKPQV
360 370 380 390 400
LRKAQAEVRA AVGDDKPRVN SEDAAKIPYL KMVVKETLRL HPPATLLVPR
410 420 430 440 450
ETMRDTTICG YDVPANTRVF VNAWAIGRDP ASWPAPDEFN PDRFVGSDVD
460 470 480 490 500
YYGSHFELIP FGAGRRICPG LTMGETNVTF TLANLLYCYD WALPGAMKPE
510 520 530
DVSMEETGAL TFHRKTPLVV VPTKYKNRRA A
Length:531
Mass (Da):59,088
Last modified:June 1, 1998 - v1
Checksum:i2272E4AA910890D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029858 mRNA. Translation: AAC39318.1.
PIRiT14640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF029858 mRNA. Translation: AAC39318.1.
PIRiT14640.

3D structure databases

ProteinModelPortaliO48958.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4558.Sb01g001180.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiO48958.

Phylogenomic databases

eggNOGiCOG2124.

Enzyme and pathway databases

UniPathwayiUPA00757; UER00745.
BioCyciMetaCyc:MONOMER-522.
SABIO-RKO48958.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome P450 in the biosynthesis of the cyanogenic glucoside dhurrin."
    Bak S., Kahn R.A., Nielsen H.L., Moeller B.L., Halkier B.A.
    Plant Mol. Biol. 36:393-405(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. SS1000.
    Tissue: Etiolated seedling.

Entry informationi

Entry nameiC71E1_SORBI
AccessioniPrimary (citable) accession number: O48958
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: January 7, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.