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O48958

- C71E1_SORBI

UniProt

O48958 - C71E1_SORBI

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Protein

4-hydroxyphenylacetaldehyde oxime monooxygenase

Gene
CYP71E1
Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of p-hydroxyphenylacetaldoxime to p-hydroxymandelonitrile. The dehydration of the oxime to the corresponding nitrile is followed by a C-hydroxylation of the nitrile to produce p-hydroxymandelonitrile.

Catalytic activityi

(Z)-4-hydroxyphenylacetaldehyde oxime + NADPH + O2 = (S)-4-hydroxymandelonitrile + NADP+ + 2 H2O.

Cofactori

Heme group By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi468 – 4681Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. 4-hydroxyphenylacetaldehyde oxime monooxygenase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. hydroxyphenylacetonitrile 2-monooxygenase activity Source: UniProtKB-EC
  4. iron ion binding Source: InterPro

GO - Biological processi

  1. dhurrin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-522.
SABIO-RKO48958.
UniPathwayiUPA00757; UER00745.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxyphenylacetaldehyde oxime monooxygenase (EC:1.14.13.68)
Alternative name(s):
Cytochrome P450 71E1
Gene namesi
Name:CYP71E1
OrganismiSorghum bicolor (Sorghum) (Sorghum vulgare)
Taxonomic identifieri4558 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeSorghum

Organism-specific databases

GrameneiO48958.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei18 – 3821Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5315314-hydroxyphenylacetaldehyde oxime monooxygenasePRO_0000052123Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi4558.Sb01g001180.1.

Structurei

3D structure databases

ProteinModelPortaliO48958.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O48958-1 [UniParc]FASTAAdd to Basket

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MATTATPQLL GGSVPQQWQT CLLVLLPVLL VSYYLLTSRS RNRSRSGKLG    50
GAPRLPPGPA QLPILGNLHL LGPLPHKNLR ELARRYGPVM QLRLGTVPTV 100
VVSSAEAARE VLKVHDVDCC SRPASPGPKR LSYDLKNVGF APYGEYWREM 150
RKLFALELLS MRRVKAACYA REQEMDRLVA DLDRAAASKA SIVLNDHVFA 200
LTDGIIGTVA FGNIYASKQF AHKERFQHVL DDAMDMMASF SAEDFFPNAA 250
GRLADRLSGF LARRERIFNE LDVFFEKVID QHMDPARPVP DNGGDLVDVL 300
INLCKEHDGT LRFTRDHVKA IVLDTFIGAI DTSSVTILWA MSELMRKPQV 350
LRKAQAEVRA AVGDDKPRVN SEDAAKIPYL KMVVKETLRL HPPATLLVPR 400
ETMRDTTICG YDVPANTRVF VNAWAIGRDP ASWPAPDEFN PDRFVGSDVD 450
YYGSHFELIP FGAGRRICPG LTMGETNVTF TLANLLYCYD WALPGAMKPE 500
DVSMEETGAL TFHRKTPLVV VPTKYKNRRA A 531
Length:531
Mass (Da):59,088
Last modified:June 1, 1998 - v1
Checksum:i2272E4AA910890D2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF029858 mRNA. Translation: AAC39318.1.
PIRiT14640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF029858 mRNA. Translation: AAC39318.1 .
PIRi T14640.

3D structure databases

ProteinModelPortali O48958.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 4558.Sb01g001180.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei O48958.

Phylogenomic databases

eggNOGi COG2124.

Enzyme and pathway databases

UniPathwayi UPA00757 ; UER00745 .
BioCyci MetaCyc:MONOMER-522.
SABIO-RK O48958.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome P450 in the biosynthesis of the cyanogenic glucoside dhurrin."
    Bak S., Kahn R.A., Nielsen H.L., Moeller B.L., Halkier B.A.
    Plant Mol. Biol. 36:393-405(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. SS1000.
    Tissue: Etiolated seedling.

Entry informationi

Entry nameiC71E1_SORBI
AccessioniPrimary (citable) accession number: O48958
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: February 19, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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