Reviewed,
UniProtKB/Swiss-Prot O48958 (C71E1_SORBI)
Last modified
November 25, 2008.
Version 58.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 4-hydroxyphenylacetaldehyde oxime monooxygenase EC=1.14.13.68 Alternative name(s): Cytochrome P450 71E1 | ||
| Gene names |
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| Organism | Sorghum bicolor (Sorghum) (Sorghum vulgare) | ||
| Taxonomic identifier | 4558 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Sorghum |
Protein attributes
| Sequence length | 531 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Catalyzes the conversion of p-hydroxyphenylacetaldoxime to p-hydroxymandelonitrile. The dehydration of the oxime to the corresponding nitrile is followed by a C-hydroxylation of the nitrile to produce p-hydroxymandelonitrile. |
| Catalytic activity | (Z)-4-hydroxyphenylacetaldehyde oxime + NADPH + O(2) = (S)-4-hydroxymandelonitrile + NADP(+) + 2 H(2)O. |
| Cofactor | Heme group By similarity. |
| Pathway | Secondary metabolite biosynthesis; dhurrin biosynthesis; dhurrin from L-tyrosine: step 2/3. |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass membrane proteinPotential. |
| Sequence similarities | Belongs to the cytochrome P450 family. |
Ontologies
Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Transmembrane |
| Ligand | Heme Iron Metal-binding NADP |
| Molecular function | Monooxygenase Oxidoreductase |
Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | 4-hydroxyphenylacetaldehyde oxime monooxygenase activity Inferred from electronic annotation. Source: EC electron carrier activityInferred from electronic annotation. Source: InterPro heme bindingInferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
Sequences
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References
| [1] | "Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome P450 in the biosynthesis of the cyanogenic glucoside dhurrin." Bak S., Kahn R.A., Nielsen H.L., Moeller B.L., Halkier B.A. Plant Mol. Biol. 36:393-405(1998) [PubMed: 9484480] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. SS1000. Tissue: Etiolated seedling. |
Cross-references
Sequence databases | |
|---|---|
| AF029858 mRNA. Translation: AAC39318.1. | |
| PIR | T14640. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1DT6 based on UniProtKB P00179. |
| ModBase | Search... |
Organism-specific databases | |
| Gramene | O48958. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MON-522. |
Family and domain databases | |
| InterPro | IPR001128. Cyt_P450. IPR002401. Cyt_P450_E_grp-I. [Graphical view] |
| Gene3D | G3DSA:1.10.630.10. Cyt_P450. 1 hit. |
| PANTHER | PTHR19383. Cyt_P450. 1 hit. |
| Pfam | PF00067. p450. 1 hit. [Graphical view] |
| PRINTS | PR00463. EP450I. PR00385. P450. |
| PROSITE | PS00086. CYTOCHROME_P450. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | C71E1_SORBI | ||||||||
| Accession | Primary (citable) accession number: O48958 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


