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O48958

- C71E1_SORBI

UniProt

O48958 - C71E1_SORBI

Protein

4-hydroxyphenylacetaldehyde oxime monooxygenase

Gene

CYP71E1

Organism
Sorghum bicolor (Sorghum) (Sorghum vulgare)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of p-hydroxyphenylacetaldoxime to p-hydroxymandelonitrile. The dehydration of the oxime to the corresponding nitrile is followed by a C-hydroxylation of the nitrile to produce p-hydroxymandelonitrile.

    Catalytic activityi

    (Z)-4-hydroxyphenylacetaldehyde oxime + NADPH + O2 = (S)-4-hydroxymandelonitrile + NADP+ + 2 H2O.

    Cofactori

    Heme group.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi468 – 4681Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. 4-hydroxyphenylacetaldehyde oxime monooxygenase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. hydroxyphenylacetonitrile 2-monooxygenase activity Source: UniProtKB-EC
    4. iron ion binding Source: InterPro

    GO - Biological processi

    1. dhurrin biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-522.
    SABIO-RKO48958.
    UniPathwayiUPA00757; UER00745.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxyphenylacetaldehyde oxime monooxygenase (EC:1.14.13.68)
    Alternative name(s):
    Cytochrome P450 71E1
    Gene namesi
    Name:CYP71E1
    OrganismiSorghum bicolor (Sorghum) (Sorghum vulgare)
    Taxonomic identifieri4558 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeSorghum

    Organism-specific databases

    GrameneiO48958.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5315314-hydroxyphenylacetaldehyde oxime monooxygenasePRO_0000052123Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi4558.Sb01g001180.1.

    Structurei

    3D structure databases

    ProteinModelPortaliO48958.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei18 – 3821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2124.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O48958-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATTATPQLL GGSVPQQWQT CLLVLLPVLL VSYYLLTSRS RNRSRSGKLG    50
    GAPRLPPGPA QLPILGNLHL LGPLPHKNLR ELARRYGPVM QLRLGTVPTV 100
    VVSSAEAARE VLKVHDVDCC SRPASPGPKR LSYDLKNVGF APYGEYWREM 150
    RKLFALELLS MRRVKAACYA REQEMDRLVA DLDRAAASKA SIVLNDHVFA 200
    LTDGIIGTVA FGNIYASKQF AHKERFQHVL DDAMDMMASF SAEDFFPNAA 250
    GRLADRLSGF LARRERIFNE LDVFFEKVID QHMDPARPVP DNGGDLVDVL 300
    INLCKEHDGT LRFTRDHVKA IVLDTFIGAI DTSSVTILWA MSELMRKPQV 350
    LRKAQAEVRA AVGDDKPRVN SEDAAKIPYL KMVVKETLRL HPPATLLVPR 400
    ETMRDTTICG YDVPANTRVF VNAWAIGRDP ASWPAPDEFN PDRFVGSDVD 450
    YYGSHFELIP FGAGRRICPG LTMGETNVTF TLANLLYCYD WALPGAMKPE 500
    DVSMEETGAL TFHRKTPLVV VPTKYKNRRA A 531
    Length:531
    Mass (Da):59,088
    Last modified:June 1, 1998 - v1
    Checksum:i2272E4AA910890D2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF029858 mRNA. Translation: AAC39318.1.
    PIRiT14640.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF029858 mRNA. Translation: AAC39318.1 .
    PIRi T14640.

    3D structure databases

    ProteinModelPortali O48958.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 4558.Sb01g001180.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei O48958.

    Phylogenomic databases

    eggNOGi COG2124.

    Enzyme and pathway databases

    UniPathwayi UPA00757 ; UER00745 .
    BioCyci MetaCyc:MONOMER-522.
    SABIO-RK O48958.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome P450 in the biosynthesis of the cyanogenic glucoside dhurrin."
      Bak S., Kahn R.A., Nielsen H.L., Moeller B.L., Halkier B.A.
      Plant Mol. Biol. 36:393-405(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. SS1000.
      Tissue: Etiolated seedling.

    Entry informationi

    Entry nameiC71E1_SORBI
    AccessioniPrimary (citable) accession number: O48958
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3