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Protein

UDP-sulfoquinovose synthase, chloroplastic

Gene

SQD1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of sulfolipids found in thylakoid membranes. Converts UDP-glucose and sulfite to the sulfolipid head group precursor UDP-sulfoquinovose.1 Publication

Catalytic activityi

UDP-alpha-D-sulfoquinovose + H2O = UDP-alpha-D-glucose + sulfite.

Cofactori

Enzyme regulationi

Concentrations above 100 µM sulfite inhibit the reaction.

Kineticsi

  1. KM=150 µM for UDP-glucose
  2. KM=10 µM for sulfite

    pH dependencei

    Optimum pH is 7.5-9.5.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei184NADCombined sources2 Publications1
    Binding sitei184SubstrateCombined sources2 Publications1
    Binding sitei202NADCombined sources2 Publications1
    Active sitei228Combined sources1 Publication1
    Binding sitei228SubstrateCombined sources1 Publication1
    Active sitei265Proton acceptorCombined sources1 Publication1
    Binding sitei265NADCombined sources2 Publications1
    Binding sitei265SubstrateCombined sources2 Publications1
    Active sitei269Combined sources1 Publication1
    Binding sitei269NADCombined sources2 Publications1
    Binding sitei292SubstrateCombined sources1 Publication1
    Binding sitei295NAD; via amide nitrogenCombined sources2 Publications1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi95 – 96NADCombined sources2 Publications2
    Nucleotide bindingi115 – 119NADCombined sources2 Publications5
    Nucleotide bindingi158 – 159NADCombined sources2 Publications2

    GO - Molecular functioni

    • coenzyme binding Source: InterPro
    • sulfotransferase activity Source: TAIR
    • UDPsulfoquinovose synthase activity Source: UniProtKB-EC
    • zinc ion binding Source: TAIR

    GO - Biological processi

    • cellular response to phosphate starvation Source: TAIR
    • glycolipid biosynthetic process Source: TAIR
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-1201.
    BRENDAi3.13.1.1. 399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-sulfoquinovose synthase, chloroplastic (EC:3.13.1.1)
    Alternative name(s):
    Sulfite:UDP-glucose sulfotransferase
    Sulfolipid biosynthesis protein
    Gene namesi
    Name:SQD1
    Ordered Locus Names:At4g33030
    ORF Names:F26P21.150
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    TAIRiAT4G33030.

    Subcellular locationi

    GO - Cellular componenti

    • chloroplast Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi228T → A: Loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 86ChloroplastCuratedAdd BLAST86
    ChainiPRO_000001046987 – 477UDP-sulfoquinovose synthase, chloroplasticAdd BLAST391

    Proteomic databases

    PaxDbiO48917.
    PRIDEiO48917.

    PTM databases

    iPTMnetiO48917.

    Expressioni

    Inductioni

    Induced in response to altered availability of inorganic phosphate.2 Publications

    Gene expression databases

    GenevisibleiO48917. AT.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi14725. 1 interactor.
    IntActiO48917. 1 interactor.
    STRINGi3702.AT4G33030.1.

    Structurei

    Secondary structure

    1477
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi86 – 90Combined sources5
    Turni91 – 93Combined sources3
    Helixi95 – 106Combined sources12
    Beta strandi110 – 115Combined sources6
    Helixi118 – 126Combined sources9
    Helixi137 – 148Combined sources12
    Beta strandi153 – 157Combined sources5
    Helixi162 – 172Combined sources11
    Beta strandi175 – 179Combined sources5
    Helixi186 – 189Combined sources4
    Helixi193 – 217Combined sources25
    Beta strandi222 – 226Combined sources5
    Helixi229 – 232Combined sources4
    Beta strandi241 – 248Combined sources8
    Beta strandi251 – 256Combined sources6
    Helixi264 – 283Combined sources20
    Beta strandi286 – 292Combined sources7
    Beta strandi294 – 296Combined sources3
    Helixi303 – 305Combined sources3
    Helixi307 – 309Combined sources3
    Turni317 – 319Combined sources3
    Helixi322 – 332Combined sources11
    Beta strandi336 – 339Combined sources4
    Beta strandi345 – 350Combined sources6
    Helixi351 – 363Combined sources13
    Beta strandi371 – 376Combined sources6
    Beta strandi378 – 382Combined sources5
    Helixi383 – 395Combined sources13
    Turni396 – 398Combined sources3
    Beta strandi403 – 406Combined sources4
    Helixi424 – 427Combined sources4
    Helixi437 – 449Combined sources13
    Helixi451 – 453Combined sources3
    Helixi456 – 458Combined sources3
    Turni465 – 467Combined sources3
    Beta strandi469 – 471Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1I24X-ray1.20A86-477[»]
    1I2BX-ray1.75A86-477[»]
    1I2CX-ray1.60A86-477[»]
    1QRRX-ray1.60A84-477[»]
    ProteinModelPortaliO48917.
    SMRiO48917.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO48917.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni322 – 325Substrate bindingCombined sources2 Publications4
    Regioni337 – 339Substrate bindingCombined sources2 Publications3
    Regioni410 – 412Substrate bindingCombined sources2 Publications3

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG1371. Eukaryota.
    COG1087. LUCA.
    HOGENOMiHOG000014371.
    InParanoidiO48917.
    KOiK06118.
    OMAiEEHYYNP.
    OrthoDBiEOG0936093X.
    PhylomeDBiO48917.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR001509. Epimerase_deHydtase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01370. Epimerase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51735. SSF51735. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O48917-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MAHLLSASCP SVISLSSSSS KNSVKPFVSG QTFFNAQLLS RSSLKGLLFQ
    60 70 80 90 100
    EKKPRKSCVF RATAVPITQQ APPETSTNNS SSKPKRVMVI GGDGYCGWAT
    110 120 130 140 150
    ALHLSKKNYE VCIVDNLVRR LFDHQLGLES LTPIASIHDR ISRWKALTGK
    160 170 180 190 200
    SIELYVGDIC DFEFLAESFK SFEPDSVVHF GEQRSAPYSM IDRSRAVYTQ
    210 220 230 240 250
    HNNVIGTLNV LFAIKEFGEE CHLVKLGTMG EYGTPNIDIE EGYITITHNG
    260 270 280 290 300
    RTDTLPYPKQ ASSFYHLSKV HDSHNIAFTC KAWGIRATDL NQGVVYGVKT
    310 320 330 340 350
    DETEMHEELR NRLDYDAVFG TALNRFCVQA AVGHPLTVYG KGGQTRGYLD
    360 370 380 390 400
    IRDTVQCVEI AIANPAKAGE FRVFNQFTEQ FSVNELASLV TKAGSKLGLD
    410 420 430 440 450
    VKKMTVPNPR VEAEEHYYNA KHTKLMELGL EPHYLSDSLL DSLLNFAVQF
    460 470
    KDRVDTKQIM PSVSWKKIGV KTKSMTT
    Length:477
    Mass (Da):53,114
    Last modified:June 1, 1998 - v1
    Checksum:i25F7304C10B025A9
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF022082 mRNA. Translation: AAB94073.1.
    AL031804 Genomic DNA. Translation: CAA21212.1.
    AL161582 Genomic DNA. Translation: CAB80020.1.
    CP002687 Genomic DNA. Translation: AEE86162.1.
    AF380641 mRNA. Translation: AAK55722.1.
    AY113071 mRNA. Translation: AAM47379.1.
    PIRiT05311.
    RefSeqiNP_195029.1. NM_119457.4.
    UniGeneiAt.2479.

    Genome annotation databases

    EnsemblPlantsiAT4G33030.1; AT4G33030.1; AT4G33030.
    GeneIDi829440.
    GrameneiAT4G33030.1; AT4G33030.1; AT4G33030.
    KEGGiath:AT4G33030.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF022082 mRNA. Translation: AAB94073.1.
    AL031804 Genomic DNA. Translation: CAA21212.1.
    AL161582 Genomic DNA. Translation: CAB80020.1.
    CP002687 Genomic DNA. Translation: AEE86162.1.
    AF380641 mRNA. Translation: AAK55722.1.
    AY113071 mRNA. Translation: AAM47379.1.
    PIRiT05311.
    RefSeqiNP_195029.1. NM_119457.4.
    UniGeneiAt.2479.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1I24X-ray1.20A86-477[»]
    1I2BX-ray1.75A86-477[»]
    1I2CX-ray1.60A86-477[»]
    1QRRX-ray1.60A84-477[»]
    ProteinModelPortaliO48917.
    SMRiO48917.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi14725. 1 interactor.
    IntActiO48917. 1 interactor.
    STRINGi3702.AT4G33030.1.

    PTM databases

    iPTMnetiO48917.

    Proteomic databases

    PaxDbiO48917.
    PRIDEiO48917.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT4G33030.1; AT4G33030.1; AT4G33030.
    GeneIDi829440.
    GrameneiAT4G33030.1; AT4G33030.1; AT4G33030.
    KEGGiath:AT4G33030.

    Organism-specific databases

    TAIRiAT4G33030.

    Phylogenomic databases

    eggNOGiKOG1371. Eukaryota.
    COG1087. LUCA.
    HOGENOMiHOG000014371.
    InParanoidiO48917.
    KOiK06118.
    OMAiEEHYYNP.
    OrthoDBiEOG0936093X.
    PhylomeDBiO48917.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-1201.
    BRENDAi3.13.1.1. 399.

    Miscellaneous databases

    EvolutionaryTraceiO48917.
    PROiO48917.

    Gene expression databases

    GenevisibleiO48917. AT.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR001509. Epimerase_deHydtase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01370. Epimerase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51735. SSF51735. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiSQD1_ARATH
    AccessioniPrimary (citable) accession number: O48917
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2003
    Last sequence update: June 1, 1998
    Last modified: November 30, 2016
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.