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O48905

- MDHC_MEDSA

UniProt

O48905 - MDHC_MEDSA

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Protein

Malate dehydrogenase, cytoplasmic

Gene

CMDH

Organism
Medicago sativa (Alfalfa)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931SubstratePROSITE-ProRule annotation
Binding sitei99 – 991SubstratePROSITE-ProRule annotation
Binding sitei106 – 1061NADBy similarity
Binding sitei113 – 1131NADBy similarity
Binding sitei132 – 1321SubstratePROSITE-ProRule annotation
Binding sitei163 – 1631SubstratePROSITE-ProRule annotation
Active sitei188 – 1881Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 187NADBy similarity
Nucleotide bindingi130 – 1323NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciARA:MONOMER-17412.
MetaCyc:MONOMER-17412.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Gene namesi
Name:CMDH
OrganismiMedicago sativa (Alfalfa)
Taxonomic identifieri3879 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Malate dehydrogenase, cytoplasmicPRO_0000113416Add
BLAST

Proteomic databases

PRIDEiO48905.
ProMEXiO48905.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO48905.
SMRiO48905. Positions 4-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O48905-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKDPVRVLV TGAAGQIGYA LVPMIARGVM LGPDQPVILH MLDIAPAAES
60 70 80 90 100
LNGVKMELVD AAFPLLKGVV ATTDVVEACT GVNIAVMVGG FPRKEGMERK
110 120 130 140 150
DVMSKNVSIY KSQASALEKH AAANCKVLVV ANPANTNALI LKEFAPSIPE
160 170 180 190 200
RNISCLTRLD HNRALGQISE RLNVQVSDVK NVIIWGNHSS TQYPDVNHAT
210 220 230 240 250
VNTPAGEKPV RQLVSDDAWL NGEFISTVQQ RGAAIIKARK LSSALSAASA
260 270 280 290 300
ACDHIRDWVL GTPQGTFVSM GVYSDGSYNV PSGLIYSFPV TCANGEWKIV
310 320 330
QGLSIDEFSR KKLDLTAEEL TEEKNLAHSC LS
Length:332
Mass (Da):35,547
Last modified:June 1, 1998 - v1
Checksum:iD9F38F868DE3D628
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF020272 mRNA. Translation: AAB99756.1.
PIRiT09291.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF020272 mRNA. Translation: AAB99756.1 .
PIRi T09291.

3D structure databases

ProteinModelPortali O48905.
SMRi O48905. Positions 4-332.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi O48905.
ProMEXi O48905.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci ARA:MONOMER-17412.
MetaCyc:MONOMER-17412.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Alfalfa malate dehydrogenase (MDH): molecular cloning and characterization of five different forms reveals a unique nodule-enhanced MDH."
    Miller S.S., Driscoll B.T., Gregerson R.G., Gantt J.S., Vance C.P.
    Plant J. 15:173-184(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Saranac.
    Tissue: Root nodule.

Entry informationi

Entry nameiMDHC_MEDSA
AccessioniPrimary (citable) accession number: O48905
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3