ID   MDHP_MEDSA              Reviewed;         437 AA.
AC   O48902;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   16-JUN-2009, entry version 75.
DE   RecName: Full=Malate dehydrogenase [NADP], chloroplastic;
DE            EC=1.1.1.82;
DE   AltName: Full=NADP-MDH;
DE   Flags: Precursor;
GN   Name=MDH1; Synonyms=P1MDH;
OS   Medicago sativa (Alfalfa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Trifolieae;
OC   Medicago.
OX   NCBI_TaxID=3879;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Saranac; TISSUE=Root nodule;
RX   MEDLINE=98388648; PubMed=9721676;
RX   DOI=10.1046/j.1365-313X.1998.00192.x;
RA   Miller S.S., Driscoll B.T., Gregerson R.G., Gantt J.S., Vance C.P.;
RT   "Alfalfa malate dehydrogenase (MDH): molecular cloning and
RT   characterization of five different forms reveals a unique nodule-
RT   enhanced MDH.";
RL   Plant J. 15:173-184(1998).
CC   -!- FUNCTION: The chloroplastic, NADP-dependent form is essential for
CC       the photosynthesis C4 cycle, which allows plants to circumvent the
CC       problem of photorespiration. In C4 plants, NADP-MDH activity acts
CC       to convert oxaloacetate to malate in chloroplasts of mesophyll
CC       cells for transport to the bundle sheath cells (By similarity).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NADP(+) = oxaloacetate + NADPH.
CC   -!- ENZYME REGULATION: Chloroplast NADP-MDH is activated upon
CC       illumination. In order to be enzymatically active, disulfides
CC       bridges on the protein must be reduced by thioredoxin which
CC       receives electrons from ferredoxin and the electron transport
CC       system of photosynthesis (By similarity).
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 2 family.
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DR   EMBL; AF020269; AAB99753.1; -; mRNA.
DR   HSSP; P46489; 1CIV.
DR   SMR; O48902; 69-434.
DR   BRENDA; 1.1.1.82; 815.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0046554; F:malate dehydrogenase (NADP+) activity; IEA:EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001236; Lactate/malate_DH.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR001252; Malate_DH_AS.
DR   InterPro; IPR011273; Malate_DH_NADP-dep_pln.
DR   InterPro; IPR010945; Malate_DH_SF1.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR23382; MDH_SF1; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   ProDom; PD003052; Mal_dehydrog; 1.
DR   TIGRFAMs; TIGR01757; Malate-DH_plant; 1.
DR   TIGRFAMs; TIGR01759; MalateDH-SF1; 1.
DR   PROSITE; PS00068; MDH; 1.
PE   2: Evidence at transcript level;
KW   Chloroplast; Disulfide bond; NADP; Oxidoreductase; Plastid;
KW   Transit peptide.
FT   TRANSIT       1     49       Chloroplast (Potential).
FT   CHAIN        50    437       Malate dehydrogenase [NADP],
FT                                chloroplastic.
FT                                /FTId=PRO_0000018644.
FT   NP_BIND     101    107       NADP (By similarity).
FT   NP_BIND     219    221       NADP (By similarity).
FT   ACT_SITE    277    277       Proton acceptor (By similarity).
FT   BINDING     182    182       Substrate (By similarity).
FT   BINDING     188    188       Substrate (By similarity).
FT   BINDING     195    195       NADP (By similarity).
FT   BINDING     202    202       NAD (By similarity).
FT   BINDING     221    221       Substrate (By similarity).
FT   BINDING     252    252       Substrate (By similarity).
FT   SITE         72     72       Activation of NADP-MDH (By similarity).
FT   SITE         77     77       Activation of NADP-MDH (By similarity).
FT   DISULFID     72     77       In oxidized inactive NAD-MDH (By
FT                                similarity).
FT   DISULFID    413    425       In oxidized inactive NAD-MDH (By
FT                                similarity).
SQ   SEQUENCE   437 AA;  47835 MW;  9C7C5377DBA5454A CRC64;
     MALTQLNNTC SKTQLHSSSQ LSFLSRTLPR HHHCTLAPLH RTQHARISCS VAPNQVQAPA
     VQTQDPKSKP DCYGVFCLTY DLKAEEETKS WKKLITIAVS GAAGMISNHL LFKLASGEVF
     GPNQPIALKL LGSERSLQAL EGVAMELEDS LFPLLREVVI SIDPYEVFQD AEWALLIGAK
     PRGPGMERAA LLDINGQIFA EQGKALNAVA SRNVKVIVVG NPCNTNALIC LKNAPNIPAK
     NFHALTRLDE NRAKCQLALK AGVFYDKVSN MTIWGNHSTT QVPDFLNARI DGLPVKEVIK
     DHKWLEEEFT EKVQKRGGAL IQKWGRSSAA STSVSIVDAI RSLIIPTPEG DWFSTGVYTT
     GNPYGIAEDI VFSMPCRSKG DGDYELVKDV IFDDYLRQKL AKTEAELLAE KKCVAHLTGE
     GIAVCDLPGD TMLPGEM
//