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Protein

Transcriptional corepressor LEUNIG_HOMOLOG

Gene

LUH

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription repressor subunit of the SEU-SLK1 and SEU-SLK2 transcriptional corepressor of abiotic stress (e.g. salt and osmotic stress) response genes, by means of an epigenetic process involving histone modification (e.g. H3K9 and H3K14 acetylation), probably by recruiting HDAC, to facilitate the condensation of chromatin thus preventing transcription at the target genes (PubMed:24564815). Can also act as a transcription activator (PubMed:21518777). Implicated in embryo and floral development (PubMed:18390806). Involved in post-synthesis cell wall modifications necessary for mucilage extrusion from seeds upon imbibition, probably by promoting the expression of genes required for mucilage maturation (e.g. MUM2) (PubMed:11706181, PubMed:21362134, PubMed:21402796, PubMed:21518777). Regulates the maintenance on leaf polarity and meristem activity as well as the initiation of embryonic shoot apical meristem (SAM) development (PubMed:19837869).7 Publications

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • cellular response to DNA damage stimulus Source: UniProtKB
  • cellular response to external biotic stimulus Source: UniProtKB
  • flower development Source: UniProtKB-KW
  • galacturonan metabolic process Source: UniProtKB
  • meristem maintenance Source: UniProtKB
  • mucilage biosynthetic process Source: UniProtKB
  • mucilage extrusion from seed coat Source: UniProtKB
  • mucilage metabolic process involved in seed coat development Source: UniProtKB
  • negative regulation of response to salt stress Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • plant-type cell wall modification Source: UniProtKB
  • polarity specification of adaxial/abaxial axis Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of embryonic development Source: UniProtKB
  • regulation of flower development Source: UniProtKB
  • regulation of leaf development Source: UniProtKB
  • regulation of response to osmotic stress Source: UniProtKB
  • regulation of shoot apical meristem development Source: UniProtKB
  • response to auxin Source: UniProtKB
  • response to bacterium Source: UniProtKB
  • response to cycloheximide Source: UniProtKB
  • response to fungus Source: UniProtKB
  • response to hypoxia Source: UniProtKB
  • response to nematode Source: UniProtKB
  • response to oxidative stress Source: UniProtKB
  • response to salt Source: UniProtKB
  • response to silver ion Source: UniProtKB
  • response to water deprivation Source: UniProtKB
  • response to wounding Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional corepressor LEUNIG_HOMOLOG
Alternative name(s):
Protein MUCILAGE-MODIFIED 11 Publication
Gene namesi
Name:LUHImported
Synonyms:MUM11 Publication
Ordered Locus Names:At2g32700Imported
ORF Names:F24L7.16Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G32700.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Strong defects in seed mucilage extrusion; no mucilage capsule formation and slightly irregular columellae in seeds. Altered structure of mucilage that accumulates abnormal levels of substituted rhamnogalacturonan I and methyl-esterified homogalacturonan. Increased amount of most sugars associated with an increase in methylation of the mucilage and/or primary cell wall pectins (PubMed:11706181, PubMed:21362134, PubMed:21402796, PubMed:21518777). Reduced MUM2 expression in seed coat and embryo. Shorter roots (PubMed:21402796). Enhanced tolerance to salt and osmotic stress conditions (PubMed:24564815). In plants lacking both LUG and LUH, embryo lethality and abnormal flowers (PubMed:18390806). Enhance the polarity and growth defects of luh/+ lug mutant leaves, being partially abaxialized (PubMed:19837869).7 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi123 – 1231S → F in luh-2;. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 787787Transcriptional corepressor LEUNIG_HOMOLOGPRO_0000433165Add
BLAST

Proteomic databases

PaxDbiO48847.
PRIDEiO48847.

PTM databases

iPTMnetiO48847.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, seedlings, apex, flowers, siliques, flower organs and seeds (including seed coat).2 Publications

Developmental stagei

Accumulates throughout young developing leaves, before being confined to the vasculature of older leaves (PubMed:19837869). Expressed during seed coat development, reaching peak expression late in differentiation at 10 days post anthesis (DPA) (PubMed:21518777).2 Publications

Inductioni

Induced by exposures to biotic stress (e.g. nematode and Botrytis cinerea) and abiotic stress (e.g. salt, genotoxic, wounding, drought and oxidative stress). Repressed by exposures to biotic stress (e.g. Agrobacterium tumefaciens) and abiotic stress (e.g. hypoxia, cycloheximide, 2,4-dichlorophenoxyacetic acid, AgNO3 and aminoethoxyvinylglycine).1 Publication

Gene expression databases

ExpressionAtlasiO48847. baseline and differential.
GenevisibleiO48847. AT.

Interactioni

Subunit structurei

Forms corepressor complexes with SLK1 and SLK2; LUH is the transcription repressor subunit and SLK1 and SLK2 the specific DNA-binding adapters (PubMed:24564815). Interacts with SEU (PubMed:18390806). Binds to YAB3, YAB5 and YAB1/FIL; these complexes promote adaxial cell identity in leaves as well as embryonic shoot apical meristem (SAM) initiation and postembryonic SAM maintenance (PubMed:19837869).3 Publications

Protein-protein interaction databases

IntActiO48847. 2 interactions.
STRINGi3702.AT2G32700.7.

Structurei

3D structure databases

ProteinModelPortaliO48847.
SMRiO48847. Positions 463-786.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 4033LisHPROSITE-ProRule annotationAdd
BLAST
Repeati508 – 54740WD 1Sequence analysisAdd
BLAST
Repeati550 – 58940WD 2Sequence analysisAdd
BLAST
Repeati593 – 63341WD 3Sequence analysisAdd
BLAST
Repeati635 – 67137WD 4Sequence analysisAdd
BLAST
Repeati675 – 71541WD 5Sequence analysisAdd
BLAST
Repeati717 – 75539WD 6Sequence analysisAdd
BLAST
Repeati757 – 78731WD 7Sequence analysisAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8888Required for SEU-bindingBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili77 – 10630Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi267 – 372106Gln-richPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 LisH domain.PROSITE-ProRule annotation
Contains 7 WD repeats.Sequence analysis

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410IQ0T. Eukaryota.
ENOG410YDHV. LUCA.
HOGENOMiHOG000082961.
OMAiSTAKAFM.
OrthoDBiEOG093604PI.
PhylomeDBiO48847.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR006594. LisH.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08513. LisH. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00667. LisH. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50896. LISH. 1 hit.
PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O48847-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQSNWEADK MLDVYIYDYL VKKKLHNTAK SFMTEGKVSP DPVAIDAPGG
60 70 80 90 100
FLFEWWSVFW DIFIARTNEK HSEAAAAYIE AQQGKAKEQQ MQIQQLQMMR
110 120 130 140 150
QAQMQRRDPN HPSLGGPMNA IGSEGMIGQS NASALAAKMY EERMKQPNPM
160 170 180 190 200
NSETSQPHLD ARMALLKSAT NHHGQIVQGN HQGGVSAALQ QIQSRTQQPT
210 220 230 240 250
EIKTEVNLGT SPRQLPVDPS TVYGQGILQS KPGMGSAGLN PGVSGLPLKG
260 270 280 290 300
WPLTGIEQMR PGLGGPQVQK SFLQNQSQFQ LSPQQQQHQM LAQVQAQGNM
310 320 330 340 350
TNSPMYGGDM DPRRFTGLPR GNLNPKDGQQ NANDGSIGSP MQSSSSKHIS
360 370 380 390 400
MPPVQQSSSQ QQDHLLSQQS QQNNRKRKGP SSSGPANSTG TGNTVGPSNS
410 420 430 440 450
QPSTPSTHTP VDGVAIAGNM HHVNSMPKGP MMYGSDGIGG LASSANQLLQ
460 470 480 490 500
DDMDQFGDVG ALEDNVESFL SQDDGDGGSL FGTLKRNSSV HTETSKPFSF
510 520 530 540 550
NEVSCIRKSA SKVICCSFSY DGKLLASAGH DKKVFIWNME TLQVESTPEE
560 570 580 590 600
HAHIITDVRF RPNSTQLATS SFDKTIKIWD ASDPGYFLRT ISGHAAPVMS
610 620 630 640 650
IDFHPKKTEL LCSCDSNNDI RFWDINASCV RAVKGASTQV RFQPRTGQFL
660 670 680 690 700
AAASENTVSI FDIENNNKRV NIFKGHSSNV HSVCWSPNGE LVASVSEDAV
710 720 730 740 750
KLWSLSSGDC IHELSNSGNK FHSVVFHPSY PDLLVIGGYQ AIELWNTMEN
760 770 780
KCMTVAGHEC VISALAQSPS TGVVASASHD KSVKIWK
Length:787
Mass (Da):85,515
Last modified:June 1, 1998 - v1
Checksum:i995B52584090CEC3
GO
Isoform 2 (identifier: O48847-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     237-237: A → AGKYINVLHCRVIAHRKENG

Note: Derived from EST data. No experimental confirmation available.Imported
Show »
Length:806
Mass (Da):87,704
Checksum:iA5857DE80AC20277
GO
Isoform 3 (identifier: O48847-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     449-450: Missing.

Note: Derived from EST data. No experimental confirmation available.Imported
Show »
Length:785
Mass (Da):85,273
Checksum:i73CBA34F4A1C972A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei237 – 2371A → AGKYINVLHCRVIAHRKENG in isoform 2. VSP_057680
Alternative sequencei449 – 4502Missing in isoform 3. VSP_057681

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003974 Genomic DNA. Translation: AAC04493.1.
CP002685 Genomic DNA. Translation: AEC08720.1.
CP002685 Genomic DNA. Translation: AEC08721.1.
CP002685 Genomic DNA. Translation: AEC08722.1.
CP002685 Genomic DNA. Translation: AEC08723.1.
CP002685 Genomic DNA. Translation: AEC08724.1.
CP002685 Genomic DNA. Translation: AEC08725.1.
CP002685 Genomic DNA. Translation: AEC08726.1.
AF367306 mRNA. Translation: AAK32893.1.
BT002219 mRNA. Translation: AAN72230.1.
AK226299 mRNA. Translation: BAE98454.1.
PIRiT00798.
RefSeqiNP_001031466.2. NM_001036389.3. [O48847-3]
NP_001189659.1. NM_001202730.1. [O48847-2]
NP_565749.1. NM_128829.3. [O48847-1]
NP_850192.1. NM_179861.3. [O48847-1]
NP_850193.1. NM_179862.2. [O48847-1]
NP_850194.1. NM_179863.2. [O48847-1]
NP_850195.2. NM_179864.3. [O48847-1]
UniGeneiAt.21002.

Genome annotation databases

EnsemblPlantsiAT2G32700.1; AT2G32700.1; AT2G32700. [O48847-1]
AT2G32700.2; AT2G32700.2; AT2G32700. [O48847-1]
AT2G32700.3; AT2G32700.3; AT2G32700. [O48847-1]
AT2G32700.4; AT2G32700.4; AT2G32700. [O48847-1]
AT2G32700.5; AT2G32700.5; AT2G32700. [O48847-1]
AT2G32700.6; AT2G32700.6; AT2G32700. [O48847-3]
AT2G32700.7; AT2G32700.7; AT2G32700. [O48847-2]
GeneIDi817830.
KEGGiath:AT2G32700.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003974 Genomic DNA. Translation: AAC04493.1.
CP002685 Genomic DNA. Translation: AEC08720.1.
CP002685 Genomic DNA. Translation: AEC08721.1.
CP002685 Genomic DNA. Translation: AEC08722.1.
CP002685 Genomic DNA. Translation: AEC08723.1.
CP002685 Genomic DNA. Translation: AEC08724.1.
CP002685 Genomic DNA. Translation: AEC08725.1.
CP002685 Genomic DNA. Translation: AEC08726.1.
AF367306 mRNA. Translation: AAK32893.1.
BT002219 mRNA. Translation: AAN72230.1.
AK226299 mRNA. Translation: BAE98454.1.
PIRiT00798.
RefSeqiNP_001031466.2. NM_001036389.3. [O48847-3]
NP_001189659.1. NM_001202730.1. [O48847-2]
NP_565749.1. NM_128829.3. [O48847-1]
NP_850192.1. NM_179861.3. [O48847-1]
NP_850193.1. NM_179862.2. [O48847-1]
NP_850194.1. NM_179863.2. [O48847-1]
NP_850195.2. NM_179864.3. [O48847-1]
UniGeneiAt.21002.

3D structure databases

ProteinModelPortaliO48847.
SMRiO48847. Positions 463-786.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO48847. 2 interactions.
STRINGi3702.AT2G32700.7.

PTM databases

iPTMnetiO48847.

Proteomic databases

PaxDbiO48847.
PRIDEiO48847.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G32700.1; AT2G32700.1; AT2G32700. [O48847-1]
AT2G32700.2; AT2G32700.2; AT2G32700. [O48847-1]
AT2G32700.3; AT2G32700.3; AT2G32700. [O48847-1]
AT2G32700.4; AT2G32700.4; AT2G32700. [O48847-1]
AT2G32700.5; AT2G32700.5; AT2G32700. [O48847-1]
AT2G32700.6; AT2G32700.6; AT2G32700. [O48847-3]
AT2G32700.7; AT2G32700.7; AT2G32700. [O48847-2]
GeneIDi817830.
KEGGiath:AT2G32700.

Organism-specific databases

TAIRiAT2G32700.

Phylogenomic databases

eggNOGiENOG410IQ0T. Eukaryota.
ENOG410YDHV. LUCA.
HOGENOMiHOG000082961.
OMAiSTAKAFM.
OrthoDBiEOG093604PI.
PhylomeDBiO48847.

Miscellaneous databases

PROiO48847.

Gene expression databases

ExpressionAtlasiO48847. baseline and differential.
GenevisibleiO48847. AT.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR006594. LisH.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08513. LisH. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00667. LisH. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50896. LISH. 1 hit.
PS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLUH_ARATH
AccessioniPrimary (citable) accession number: O48847
Secondary accession number(s): F4ITU4
, F4ITV0, Q0WWP4, Q3EBQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 24, 2015
Last sequence update: June 1, 1998
Last modified: September 7, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.