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Protein

Cytochrome b5 isoform B

Gene

CYTB5-B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases, including fatty acid desaturases.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi64 – 641Iron (heme axial ligand)PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • alkane biosynthetic process Source: TAIR
  • oxidation-reduction process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-ATH-196836. Vitamin C (ascorbate) metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5 isoform B1 Publication
Short name:
AtCb5-B1 Publication
Alternative name(s):
Cytochrome b5 isoform 2
Cytochrome b5 isoform E1 Publication
Short name:
AtCb5-E1 Publication
Gene namesi
Name:CYTB5-BCurated
Synonyms:CB5-B1 Publication, CB5-E1 Publication
Ordered Locus Names:At2g32720
ORF Names:F24L7.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G32720.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei107 – 12721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 134134Cytochrome b5 isoform BPRO_0000166021Add
BLAST

Post-translational modificationi

Ubiquitinated.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiO48845.
PRIDEiO48845.

Expressioni

Gene expression databases

GenevisibleiO48845. AT.

Interactioni

Subunit structurei

Interacts with CER1, FAH1, FAH2 and BI-1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BI-1Q9LD455EBI-2295402,EBI-1644586
FAH2Q9SUC53EBI-2295402,EBI-2296064

Protein-protein interaction databases

BioGridi3179. 10 interactions.
IntActiO48845. 3 interactions.
STRINGi3702.AT2G32720.1.

Structurei

3D structure databases

ProteinModelPortaliO48845.
SMRiO48845. Positions 3-95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 8177Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
HOGENOMiHOG000039853.
InParanoidiO48845.
OMAiVAKHTSK.
PhylomeDBiO48845.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O48845-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDEAKIFTL SEVSEHNQAH DCWIVINGKV YNVTKFLEDH PGGDDVLLSS
60 70 80 90 100
TGKDATDDFE DVGHSESARE MMEQYYVGEI DPTTIPKKVK YTPPKQPHYN
110 120 130
QDKTSEFIIK LLQFLVPLAI LGLAVGIRIY TKSG
Length:134
Mass (Da):15,016
Last modified:June 1, 1998 - v1
Checksum:iB405F5430F5716C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003974 Genomic DNA. Translation: AAC04491.1.
CP002685 Genomic DNA. Translation: AEC08729.1.
AY128405 mRNA. Translation: AAM91608.1.
BT000085 mRNA. Translation: AAN15404.1.
AK227415 mRNA. Translation: BAE99419.1.
PIRiT00796.
RefSeqiNP_180831.1. NM_128831.3.
UniGeneiAt.19477.

Genome annotation databases

EnsemblPlantsiAT2G32720.1; AT2G32720.1; AT2G32720.
GeneIDi817832.
GrameneiAT2G32720.1; AT2G32720.1; AT2G32720.
KEGGiath:AT2G32720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003974 Genomic DNA. Translation: AAC04491.1.
CP002685 Genomic DNA. Translation: AEC08729.1.
AY128405 mRNA. Translation: AAM91608.1.
BT000085 mRNA. Translation: AAN15404.1.
AK227415 mRNA. Translation: BAE99419.1.
PIRiT00796.
RefSeqiNP_180831.1. NM_128831.3.
UniGeneiAt.19477.

3D structure databases

ProteinModelPortaliO48845.
SMRiO48845. Positions 3-95.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3179. 10 interactions.
IntActiO48845. 3 interactions.
STRINGi3702.AT2G32720.1.

Proteomic databases

PaxDbiO48845.
PRIDEiO48845.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G32720.1; AT2G32720.1; AT2G32720.
GeneIDi817832.
GrameneiAT2G32720.1; AT2G32720.1; AT2G32720.
KEGGiath:AT2G32720.

Organism-specific databases

TAIRiAT2G32720.

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
HOGENOMiHOG000039853.
InParanoidiO48845.
OMAiVAKHTSK.
PhylomeDBiO48845.

Enzyme and pathway databases

ReactomeiR-ATH-196836. Vitamin C (ascorbate) metabolism.

Miscellaneous databases

PROiO48845.

Gene expression databases

GenevisibleiO48845. AT.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Functional association of cell death suppressor, Arabidopsis Bax inhibitor-1, with fatty acid 2-hydroxylation through cytochrome b(5)."
    Nagano M., Ihara-Ohori Y., Imai H., Inada N., Fujimoto M., Tsutsumi N., Uchimiya H., Kawai-Yamada M.
    Plant J. 58:122-134(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH BI-1; FAH1 AND FAH2, NOMENCLATURE.
  6. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
    Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
    Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
  7. "Reconstitution of plant alkane biosynthesis in yeast demonstrates that Arabidopsis ECERIFERUM1 and ECERIFERUM3 are core components of a very-long-chain alkane synthesis complex."
    Bernard A., Domergue F., Pascal S., Jetter R., Renne C., Faure J.D., Haslam R.P., Napier J.A., Lessire R., Joubes J.
    Plant Cell 24:3106-3118(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CER1, SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
  8. "Higher plant cytochrome b5 polypeptides modulate fatty acid desaturation."
    Kumar R., Tran L.S., Neelakandan A.K., Nguyen H.T.
    PLoS ONE 7:E31370-E31370(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, NOMENCLATURE.

Entry informationi

Entry nameiCYB5B_ARATH
AccessioniPrimary (citable) accession number: O48845
Secondary accession number(s): Q0WTX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: June 1, 1998
Last modified: May 11, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.