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O48845 (CYB52_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable cytochrome b5 isoform 2
Gene names
Ordered Locus Names:At2g32720
ORF Names:F24L7.14
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length134 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases By similarity.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Microsome membrane; Single-pass membrane protein; Cytoplasmic side By similarity.

Post-translational modification

Ubiquitinated. Ref.4

Sequence similarities

Belongs to the cytochrome b5 family.

Contains 1 cytochrome b5 heme-binding domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BI-1Q9LD455EBI-2295402,EBI-1644586
T13K14.30Q9SUC53EBI-2295402,EBI-2296064

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 134134Probable cytochrome b5 isoform 2
PRO_0000166021

Regions

Transmembrane107 – 12721Helical; Potential
Domain5 – 8177Cytochrome b5 heme-binding

Sites

Metal binding401Iron (heme axial ligand) By similarity
Metal binding641Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
O48845 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: B405F5430F5716C1

FASTA13415,016
        10         20         30         40         50         60 
MGDEAKIFTL SEVSEHNQAH DCWIVINGKV YNVTKFLEDH PGGDDVLLSS TGKDATDDFE 

        70         80         90        100        110        120 
DVGHSESARE MMEQYYVGEI DPTTIPKKVK YTPPKQPHYN QDKTSEFIIK LLQFLVPLAI 

       130 
LGLAVGIRIY TKSG 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
Plant J. 59:344-358(2009) [PubMed: 19292762] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC003974 Genomic DNA. Translation: AAC04491.1.
CP002685 Genomic DNA. Translation: AEC08729.1.
AY128405 mRNA. Translation: AAM91608.1.
BT000085 mRNA. Translation: AAN15404.1.
IPIIPI00534013.
PIRT00796.
RefSeqNP_180831.1. NM_128831.3.
UniGeneAt.19477.

3D structure databases

ProteinModelPortalO48845.
SMRO48845. Positions 2-85.
ModBaseSearch...

Protein-protein interaction databases

IntActO48845. 3 interactions.
STRINGO48845.

Proteomic databases

PRIDEO48845.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G32720.1; AT2G32720.1; AT2G32720.
GeneID817832.
GenomeReviewsGene locus AT2G32720 in contig CT485783_GR.
KEGGath:AT2G32720.

Organism-specific databases

TAIRAt2g32720.

Phylogenomic databases

eggNOGKOG0537.
HOGENOMHBG629516.
InParanoidO48845.
OMAREMMEQY.
PhylomeDBO48845.
ProtClustDBCLSN2913062.

Gene expression databases

ArrayExpressO48845.
GenevestigatorO48845.
GermOnlineAT2G32720. Arabidopsis thaliana.

Family and domain databases

InterProIPR001199. Cyt_B5.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
Gene3DG3DSA:3.10.120.10. Cyt_B5. 1 hit.
PfamPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSPR00363. CYTOCHROMEB5.
SUPFAMSSF55856. Cyt_B5. 1 hit.
PROSITEPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYB52_ARATH
AccessionPrimary (citable) accession number: O48845
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: June 1, 1998
Last modified: September 21, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families