Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O48840

- PPA13_ARATH

UniProt

O48840 - PPA13_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Purple acid phosphatase 13

Gene

PAP13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi203 – 2031IronBy similarity
Metal bindingi233 – 2331IronBy similarity
Active sitei389 – 3891Proton donorBy similarity
Metal bindingi416 – 4161ZincBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: TAIR
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT2G32770-MONOMER.
ARA:GQT-2796-MONOMER.
ARA:GQT-2797-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 13 (EC:3.1.3.2)
Gene namesi
Name:PAP13
Synonyms:AT2
Ordered Locus Names:At2g32770
ORF Names:F24L7.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G32770.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 545520Purple acid phosphatase 13PRO_0000372817Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi125 – 1251N-linked (GlcNAc...)Sequence Analysis
Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi209 – 2091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence Analysis
Glycosylationi321 – 3211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi351 – 3511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi367 – 3671N-linked (GlcNAc...)Sequence Analysis
Glycosylationi428 – 4281N-linked (GlcNAc...)Sequence Analysis
Glycosylationi466 – 4661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi475 – 4751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi510 – 5101N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiO48840.

Expressioni

Tissue specificityi

Expressed in stems, leaves, flowers and siliques.1 Publication

Gene expression databases

GenevestigatoriO48840.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO48840.
SMRiO48840. Positions 51-537.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni416 – 4183Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG265012.
HOGENOMiHOG000238330.
InParanoidiO48840.
OMAiSAMSKEY.
PhylomeDBiO48840.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 3 hits.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 2 (identifier: O48840-2) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVVKYTMSMS FFVIFASTVT IIVHGFPSTL DGPLNPVTAP LDPNLNPIAF
60 70 80 90 100
DLPESDPSFV KPISEFLLPE QISVSLSYSF DSVWISWVTG EYQIGEKDSA
110 120 130 140 150
PLDPNCVQSI VQYREFDVRR TRKQNATGHS IVYNQQYSSE NGFMNYTSGI
160 170 180 190 200
IHHVQLTGLK PNTLYRYQCG DPSLSAMSKE YYFRTMPKST SENYPHRIVV
210 220 230 240 250
AGDLGLTYNT STVLGHILSN HPDLVVLLGG FSYADTYLAN KTKLDCSSCH
260 270 280 290 300
CDQNGTSSDC GSCYSSGETY QPRWDYWGRF MEPLTANVPT MMVAGEHEIE
310 320 330 340 350
PQTENNLTFA AYSSRFAFPS NESGSFSPLY YSFNAGGAHF IVLNSYTLYD
360 370 380 390 400
NSSDQYIWLE SDLIKINRSE TPWVVATWSL PWYSTFKGHY REAESMRIHL
410 420 430 440 450
EDLLYNYRVD IVFNSHVDAY ERSNRVYNYT LDQCGPVYIT TGAGGAGKLE
460 470 480 490 500
TQHVDDPGNI PDPSQNYSCR SSGLNSTLEP VKDETCPVKQ PEYSAYRESS
510 520 530 540
FGFGILEVKN ETHALWSWNR NQDLYYLAAD VIHIVRQPEM CSVCN

Note: No experimental confirmation available.

Length:545
Mass (Da):61,543
Last modified:September 22, 2009 - v2
Checksum:i4E35C8DCDD2B293C
GO
Isoform 1 (identifier: O48840-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     324-353: GSFSPLYYSFNAGGAHFIVLNSYTLYDNSS → A

Note: May be due to a competing donor splice site. No experimental confirmation available.

Show »
Length:516
Mass (Da):58,339
Checksum:iB068B6603EA100DE
GO
Isoform 3 (identifier: O48840-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-88: MVVKYTMSMS...SFDSVWISWV → MVVVLYT
     509-509: K → S
     510-545: Missing.

Note: May be due to introns retention. No experimental confirmation available.Curated

Show »
Length:428
Mass (Da):48,313
Checksum:i8352AE4B96D45D33
GO

Sequence cautioni

The sequence BX818780 differs from that shown. Reason: Frameshift at position 427. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti384 – 3841S → R in BX818780. (PubMed:14993207)Curated
Sequence conflicti387 – 3882KG → TR in BX818780. (PubMed:14993207)Curated
Sequence conflicti392 – 3921E → D in BX818780. (PubMed:14993207)Curated
Sequence conflicti402 – 4021D → N in BX818780. (PubMed:14993207)Curated
Isoform 3 (identifier: O48840-3)
Sequence conflicti47 – 471G → D in AAM16284. (PubMed:14993207)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8888MVVKY…WISWV → MVVVLYT in isoform 3. 1 PublicationVSP_037193Add
BLAST
Alternative sequencei324 – 35330GSFSP…YDNSS → A in isoform 1. 1 PublicationVSP_038047Add
BLAST
Alternative sequencei509 – 5091K → S in isoform 3. 1 PublicationVSP_037196
Alternative sequencei510 – 54536Missing in isoform 3. 1 PublicationVSP_037197Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492665 mRNA. Translation: AAM15914.1.
AC003974 Genomic DNA. Translation: AAC04486.1.
CP002685 Genomic DNA. Translation: AEC08736.1.
CP002685 Genomic DNA. Translation: AEC08737.1.
CP002685 Genomic DNA. Translation: AEC08738.1.
AY090894 mRNA. Translation: AAM16284.1.
BX818780 mRNA. No translation available.
PIRiT00791.
RefSeqiNP_180836.1. NM_128837.1. [O48840-1]
NP_850198.1. NM_179867.1. [O48840-3]
NP_973585.1. NM_201856.3. [O48840-2]
UniGeneiAt.38023.

Genome annotation databases

EnsemblPlantsiAT2G32770.3; AT2G32770.3; AT2G32770. [O48840-2]
GeneIDi817838.
KEGGiath:AT2G32770.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492665 mRNA. Translation: AAM15914.1 .
AC003974 Genomic DNA. Translation: AAC04486.1 .
CP002685 Genomic DNA. Translation: AEC08736.1 .
CP002685 Genomic DNA. Translation: AEC08737.1 .
CP002685 Genomic DNA. Translation: AEC08738.1 .
AY090894 mRNA. Translation: AAM16284.1 .
BX818780 mRNA. No translation available.
PIRi T00791.
RefSeqi NP_180836.1. NM_128837.1. [O48840-1 ]
NP_850198.1. NM_179867.1. [O48840-3 ]
NP_973585.1. NM_201856.3. [O48840-2 ]
UniGenei At.38023.

3D structure databases

ProteinModelPortali O48840.
SMRi O48840. Positions 51-537.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi O48840.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G32770.3 ; AT2G32770.3 ; AT2G32770 . [O48840-2 ]
GeneIDi 817838.
KEGGi ath:AT2G32770.

Organism-specific databases

TAIRi AT2G32770.

Phylogenomic databases

eggNOGi NOG265012.
HOGENOMi HOG000238330.
InParanoidi O48840.
OMAi SAMSKEY.
PhylomeDBi O48840.

Enzyme and pathway databases

BioCyci ARA:AT2G32770-MONOMER.
ARA:GQT-2796-MONOMER.
ARA:GQT-2797-MONOMER.

Gene expression databases

Genevestigatori O48840.

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 2 hits.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
    Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
    J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), GENE FAMILY, NOMENCLATURE.
    Strain: cv. Col-1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
    Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
    Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiPPA13_ARATH
AccessioniPrimary (citable) accession number: O48840
Secondary accession number(s): Q3EBP9, Q8RX38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 22, 2009
Last modified: November 26, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3