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O48840

- PPA13_ARATH

UniProt

O48840 - PPA13_ARATH

Protein

Purple acid phosphatase 13

Gene

PAP13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 2 (22 Sep 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.

    Cofactori

    Binds 1 iron ion per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi203 – 2031IronBy similarity
    Metal bindingi233 – 2331IronBy similarity
    Active sitei389 – 3891Proton donorBy similarity
    Metal bindingi416 – 4161ZincBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: TAIR
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciARA:AT2G32770-MONOMER.
    ARA:GQT-2796-MONOMER.
    ARA:GQT-2797-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Purple acid phosphatase 13 (EC:3.1.3.2)
    Gene namesi
    Name:PAP13
    Synonyms:AT2
    Ordered Locus Names:At2g32770
    ORF Names:F24L7.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G32770.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 545520Purple acid phosphatase 13PRO_0000372817Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi125 – 1251N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi209 – 2091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi254 – 2541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi306 – 3061N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi321 – 3211N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi351 – 3511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi367 – 3671N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi428 – 4281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi466 – 4661N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi475 – 4751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi510 – 5101N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiO48840.

    Expressioni

    Tissue specificityi

    Expressed in stems, leaves, flowers and siliques.1 Publication

    Gene expression databases

    GenevestigatoriO48840.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO48840.
    SMRiO48840. Positions 51-537.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni416 – 4183Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG265012.
    HOGENOMiHOG000238330.
    InParanoidiQ3EBP9.
    OMAiSAMSKEY.
    PhylomeDBiO48840.

    Family and domain databases

    Gene3Di2.60.40.380. 1 hit.
    3.60.21.10. 2 hits.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 3 hits.

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 2 (identifier: O48840-2) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVVKYTMSMS FFVIFASTVT IIVHGFPSTL DGPLNPVTAP LDPNLNPIAF    50
    DLPESDPSFV KPISEFLLPE QISVSLSYSF DSVWISWVTG EYQIGEKDSA 100
    PLDPNCVQSI VQYREFDVRR TRKQNATGHS IVYNQQYSSE NGFMNYTSGI 150
    IHHVQLTGLK PNTLYRYQCG DPSLSAMSKE YYFRTMPKST SENYPHRIVV 200
    AGDLGLTYNT STVLGHILSN HPDLVVLLGG FSYADTYLAN KTKLDCSSCH 250
    CDQNGTSSDC GSCYSSGETY QPRWDYWGRF MEPLTANVPT MMVAGEHEIE 300
    PQTENNLTFA AYSSRFAFPS NESGSFSPLY YSFNAGGAHF IVLNSYTLYD 350
    NSSDQYIWLE SDLIKINRSE TPWVVATWSL PWYSTFKGHY REAESMRIHL 400
    EDLLYNYRVD IVFNSHVDAY ERSNRVYNYT LDQCGPVYIT TGAGGAGKLE 450
    TQHVDDPGNI PDPSQNYSCR SSGLNSTLEP VKDETCPVKQ PEYSAYRESS 500
    FGFGILEVKN ETHALWSWNR NQDLYYLAAD VIHIVRQPEM CSVCN 545

    Note: No experimental confirmation available.

    Length:545
    Mass (Da):61,543
    Last modified:September 22, 2009 - v2
    Checksum:i4E35C8DCDD2B293C
    GO
    Isoform 1 (identifier: O48840-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         324-353: GSFSPLYYSFNAGGAHFIVLNSYTLYDNSS → A

    Note: May be due to a competing donor splice site. No experimental confirmation available.

    Show »
    Length:516
    Mass (Da):58,339
    Checksum:iB068B6603EA100DE
    GO
    Isoform 3 (identifier: O48840-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-88: MVVKYTMSMS...SFDSVWISWV → MVVVLYT
         509-509: K → S
         510-545: Missing.

    Note: May be due to introns retention. No experimental confirmation available.Curated

    Show »
    Length:428
    Mass (Da):48,313
    Checksum:i8352AE4B96D45D33
    GO

    Sequence cautioni

    The sequence BX818780 differs from that shown. Reason: Frameshift at position 427.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti384 – 3841S → R in BX818780. (PubMed:14993207)Curated
    Sequence conflicti387 – 3882KG → TR in BX818780. (PubMed:14993207)Curated
    Sequence conflicti392 – 3921E → D in BX818780. (PubMed:14993207)Curated
    Sequence conflicti402 – 4021D → N in BX818780. (PubMed:14993207)Curated
    Isoform 3 (identifier: O48840-3)
    Sequence conflicti47 – 471G → D in AAM16284. (PubMed:14993207)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 8888MVVKY…WISWV → MVVVLYT in isoform 3. 1 PublicationVSP_037193Add
    BLAST
    Alternative sequencei324 – 35330GSFSP…YDNSS → A in isoform 1. 1 PublicationVSP_038047Add
    BLAST
    Alternative sequencei509 – 5091K → S in isoform 3. 1 PublicationVSP_037196
    Alternative sequencei510 – 54536Missing in isoform 3. 1 PublicationVSP_037197Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF492665 mRNA. Translation: AAM15914.1.
    AC003974 Genomic DNA. Translation: AAC04486.1.
    CP002685 Genomic DNA. Translation: AEC08736.1.
    CP002685 Genomic DNA. Translation: AEC08737.1.
    CP002685 Genomic DNA. Translation: AEC08738.1.
    AY090894 mRNA. Translation: AAM16284.1.
    BX818780 mRNA. No translation available.
    PIRiT00791.
    RefSeqiNP_180836.1. NM_128837.1. [O48840-1]
    NP_850198.1. NM_179867.1. [O48840-3]
    NP_973585.1. NM_201856.3. [O48840-2]
    UniGeneiAt.38023.

    Genome annotation databases

    EnsemblPlantsiAT2G32770.3; AT2G32770.3; AT2G32770. [O48840-2]
    GeneIDi817838.
    KEGGiath:AT2G32770.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF492665 mRNA. Translation: AAM15914.1 .
    AC003974 Genomic DNA. Translation: AAC04486.1 .
    CP002685 Genomic DNA. Translation: AEC08736.1 .
    CP002685 Genomic DNA. Translation: AEC08737.1 .
    CP002685 Genomic DNA. Translation: AEC08738.1 .
    AY090894 mRNA. Translation: AAM16284.1 .
    BX818780 mRNA. No translation available.
    PIRi T00791.
    RefSeqi NP_180836.1. NM_128837.1. [O48840-1 ]
    NP_850198.1. NM_179867.1. [O48840-3 ]
    NP_973585.1. NM_201856.3. [O48840-2 ]
    UniGenei At.38023.

    3D structure databases

    ProteinModelPortali O48840.
    SMRi O48840. Positions 51-537.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O48840.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G32770.3 ; AT2G32770.3 ; AT2G32770 . [O48840-2 ]
    GeneIDi 817838.
    KEGGi ath:AT2G32770.

    Organism-specific databases

    TAIRi AT2G32770.

    Phylogenomic databases

    eggNOGi NOG265012.
    HOGENOMi HOG000238330.
    InParanoidi Q3EBP9.
    OMAi SAMSKEY.
    PhylomeDBi O48840.

    Enzyme and pathway databases

    BioCyci ARA:AT2G32770-MONOMER.
    ARA:GQT-2796-MONOMER.
    ARA:GQT-2797-MONOMER.

    Gene expression databases

    Genevestigatori O48840.

    Family and domain databases

    Gene3Di 2.60.40.380. 1 hit.
    3.60.21.10. 2 hits.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 3 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
      Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
      J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), GENE FAMILY, NOMENCLATURE.
      Strain: cv. Col-1.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
      Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
      Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Columbia.
    5. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
      Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
      Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPPA13_ARATH
    AccessioniPrimary (citable) accession number: O48840
    Secondary accession number(s): Q3EBP9, Q8RX38
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: September 22, 2009
    Last modified: October 1, 2014
    This is version 93 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3