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Protein

Purple acid phosphatase 13

Gene

PAP13

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi203IronBy similarity1
Metal bindingi233IronBy similarity1
Active sitei389Proton donorBy similarity1
Metal bindingi416ZincBy similarity1

GO - Molecular functioni

  • acid phosphatase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
LigandIron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT2G32770-MONOMER
BRENDAi3.1.3.2 399

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 13 (EC:3.1.3.2)
Gene namesi
Name:PAP13
Synonyms:AT2
Ordered Locus Names:At2g32770
ORF Names:F24L7.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G32770
TAIRilocus:2046407 AT2G32770

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000037281726 – 545Purple acid phosphatase 13Add BLAST520

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi125N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi145N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi209N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi240N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi254N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi306N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi321N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi351N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi367N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi428N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi466N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi475N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi510N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO48840

Expressioni

Tissue specificityi

Expressed in stems, leaves, flowers and siliques.1 Publication

Gene expression databases

ExpressionAtlasiO48840 baseline and differential
GenevisibleiO48840 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT2G32770.3

Structurei

3D structure databases

ProteinModelPortaliO48840
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni416 – 418Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1378 Eukaryota
COG1409 LUCA
HOGENOMiHOG000238330
InParanoidiO48840
KOiK22390
OMAiVQSIVQY
OrthoDBiEOG093608JS
PhylomeDBiO48840

Family and domain databases

Gene3Di2.60.40.380, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR008963 Purple_acid_Pase-like_N
IPR015914 Purple_acid_Pase_N
IPR025733 Purple_acid_PPase_C_dom
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PF14008 Metallophos_C, 1 hit
PF16656 Pur_ac_phosph_N, 1 hit
SUPFAMiSSF49363 SSF49363, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: O48840-2) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVVKYTMSMS FFVIFASTVT IIVHGFPSTL DGPLNPVTAP LDPNLNPIAF
60 70 80 90 100
DLPESDPSFV KPISEFLLPE QISVSLSYSF DSVWISWVTG EYQIGEKDSA
110 120 130 140 150
PLDPNCVQSI VQYREFDVRR TRKQNATGHS IVYNQQYSSE NGFMNYTSGI
160 170 180 190 200
IHHVQLTGLK PNTLYRYQCG DPSLSAMSKE YYFRTMPKST SENYPHRIVV
210 220 230 240 250
AGDLGLTYNT STVLGHILSN HPDLVVLLGG FSYADTYLAN KTKLDCSSCH
260 270 280 290 300
CDQNGTSSDC GSCYSSGETY QPRWDYWGRF MEPLTANVPT MMVAGEHEIE
310 320 330 340 350
PQTENNLTFA AYSSRFAFPS NESGSFSPLY YSFNAGGAHF IVLNSYTLYD
360 370 380 390 400
NSSDQYIWLE SDLIKINRSE TPWVVATWSL PWYSTFKGHY REAESMRIHL
410 420 430 440 450
EDLLYNYRVD IVFNSHVDAY ERSNRVYNYT LDQCGPVYIT TGAGGAGKLE
460 470 480 490 500
TQHVDDPGNI PDPSQNYSCR SSGLNSTLEP VKDETCPVKQ PEYSAYRESS
510 520 530 540
FGFGILEVKN ETHALWSWNR NQDLYYLAAD VIHIVRQPEM CSVCN
Note: No experimental confirmation available.
Length:545
Mass (Da):61,543
Last modified:September 22, 2009 - v2
Checksum:i4E35C8DCDD2B293C
GO
Isoform 1 (identifier: O48840-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     324-353: GSFSPLYYSFNAGGAHFIVLNSYTLYDNSS → A

Note: May be due to a competing donor splice site. No experimental confirmation available.
Show »
Length:516
Mass (Da):58,339
Checksum:iB068B6603EA100DE
GO
Isoform 3 (identifier: O48840-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-88: MVVKYTMSMS...SFDSVWISWV → MVVVLYT
     509-509: K → S
     510-545: Missing.

Note: May be due to introns retention. No experimental confirmation available.Curated
Show »
Length:428
Mass (Da):48,313
Checksum:i8352AE4B96D45D33
GO

Sequence cautioni

The sequence BX818780 differs from that shown. Reason: Frameshift at position 427.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti384S → R in BX818780 (PubMed:14993207).Curated1
Sequence conflicti387 – 388KG → TR in BX818780 (PubMed:14993207).Curated2
Sequence conflicti392E → D in BX818780 (PubMed:14993207).Curated1
Sequence conflicti402D → N in BX818780 (PubMed:14993207).Curated1
Isoform 3 (identifier: O48840-3)
Sequence conflicti47G → D in AAM16284 (PubMed:14993207).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0371931 – 88MVVKY…WISWV → MVVVLYT in isoform 3. 1 PublicationAdd BLAST88
Alternative sequenceiVSP_038047324 – 353GSFSP…YDNSS → A in isoform 1. 1 PublicationAdd BLAST30
Alternative sequenceiVSP_037196509K → S in isoform 3. 1 Publication1
Alternative sequenceiVSP_037197510 – 545Missing in isoform 3. 1 PublicationAdd BLAST36

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF492665 mRNA Translation: AAM15914.1
AC003974 Genomic DNA Translation: AAC04486.1
CP002685 Genomic DNA Translation: AEC08736.1
CP002685 Genomic DNA Translation: AEC08737.1
CP002685 Genomic DNA Translation: AEC08738.1
AY090894 mRNA Translation: AAM16284.1
BX818780 mRNA No translation available.
PIRiT00791
RefSeqiNP_180836.1, NM_128837.1 [O48840-1]
NP_850198.1, NM_179867.1 [O48840-3]
NP_973585.1, NM_201856.4 [O48840-2]
UniGeneiAt.38023

Genome annotation databases

EnsemblPlantsiAT2G32770.1; AT2G32770.1; AT2G32770 [O48840-1]
AT2G32770.2; AT2G32770.2; AT2G32770 [O48840-3]
AT2G32770.3; AT2G32770.3; AT2G32770 [O48840-2]
GeneIDi817838
GrameneiAT2G32770.1; AT2G32770.1; AT2G32770 [O48840-1]
AT2G32770.2; AT2G32770.2; AT2G32770 [O48840-3]
AT2G32770.3; AT2G32770.3; AT2G32770 [O48840-2]
KEGGiath:AT2G32770

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPPA13_ARATH
AccessioniPrimary (citable) accession number: O48840
Secondary accession number(s): Q3EBP9, Q8RX38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 22, 2009
Last modified: April 25, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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