O48840 (PPA13_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Purple acid phosphatase 13
EC=3.1.3.2

Gene names

Name:	PAP13
Synonyms:	AT2
Ordered Locus Names:	At2g32770
ORF Names:	F24L7.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed in stems, leaves, flowers and siliques. Ref.5
Sequence similarities	Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.
Sequence caution	The sequence BX818780 differs from that shown. Reason: Frameshift at position 427.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acid phosphatase activity Inferred from sequence or structural similarity Ref.1Ref.5. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: O48840-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 1 (identifier: O48840-1)

The sequence of this isoform differs from the canonical sequence as follows:
     324-353: GSFSPLYYSFNAGGAHFIVLNSYTLYDNSS → A

Note: May be due to a competing donor splice site. No experimental confirmation available.

Isoform 3 (identifier: O48840-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-88: MVVKYTMSMS...SFDSVWISWV → MVVVLYT
     509-509: K → S
     510-545: Missing.

Note: May be due to introns retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 545	520	Purple acid phosphatase 13		PRO_0000372817
Regions
	Region	416 – 418	3	Substrate binding By similarity
Sites
	Active site	389	1	Proton donor By similarity
	Metal binding	203	1	Iron By similarity
	Metal binding	233	1	Iron By similarity
	Metal binding	416	1	Zinc By similarity
Amino acid modifications
	Glycosylation	125	1	N-linked (GlcNAc...) Potential
	Glycosylation	145	1	N-linked (GlcNAc...) Potential
	Glycosylation	209	1	N-linked (GlcNAc...) Potential
	Glycosylation	240	1	N-linked (GlcNAc...) Potential
	Glycosylation	254	1	N-linked (GlcNAc...) Potential
	Glycosylation	306	1	N-linked (GlcNAc...) Potential
	Glycosylation	321	1	N-linked (GlcNAc...) Potential
	Glycosylation	351	1	N-linked (GlcNAc...) Potential
	Glycosylation	367	1	N-linked (GlcNAc...) Potential
	Glycosylation	428	1	N-linked (GlcNAc...) Potential
	Glycosylation	466	1	N-linked (GlcNAc...) Potential
	Glycosylation	475	1	N-linked (GlcNAc...) Potential
	Glycosylation	510	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 88	88	MVVKY…WISWV → MVVVLYT in isoform 3.		VSP_037193
	Alternative sequence	324 – 353	30	GSFSP…YDNSS → A in isoform 1.		VSP_038047
	Alternative sequence	509	1	K → S in isoform 3.		VSP_037196
	Alternative sequence	510 – 545	36	Missing in isoform 3.		VSP_037197
Experimental info
	Sequence conflict	384	1	S → R in BX818780. Ref.4
	Sequence conflict	387 – 388	2	KG → TR in BX818780. Ref.4
	Sequence conflict	392	1	E → D in BX818780. Ref.4
	Sequence conflict	402	1	D → N in BX818780. Ref.4
Isoform 3:
	Sequence conflict	47	1	G → D in AAM16284. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 [UniParc]. Last modified September 22, 2009. Version 2. Checksum: 4E35C8DCDD2B293C	FASTA	545	61,543
	10 20 30 40 50 60 MVVKYTMSMS FFVIFASTVT IIVHGFPSTL DGPLNPVTAP LDPNLNPIAF DLPESDPSFV 70 80 90 100 110 120 KPISEFLLPE QISVSLSYSF DSVWISWVTG EYQIGEKDSA PLDPNCVQSI VQYREFDVRR 130 140 150 160 170 180 TRKQNATGHS IVYNQQYSSE NGFMNYTSGI IHHVQLTGLK PNTLYRYQCG DPSLSAMSKE 190 200 210 220 230 240 YYFRTMPKST SENYPHRIVV AGDLGLTYNT STVLGHILSN HPDLVVLLGG FSYADTYLAN 250 260 270 280 290 300 KTKLDCSSCH CDQNGTSSDC GSCYSSGETY QPRWDYWGRF MEPLTANVPT MMVAGEHEIE 310 320 330 340 350 360 PQTENNLTFA AYSSRFAFPS NESGSFSPLY YSFNAGGAHF IVLNSYTLYD NSSDQYIWLE 370 380 390 400 410 420 SDLIKINRSE TPWVVATWSL PWYSTFKGHY REAESMRIHL EDLLYNYRVD IVFNSHVDAY 430 440 450 460 470 480 ERSNRVYNYT LDQCGPVYIT TGAGGAGKLE TQHVDDPGNI PDPSQNYSCR SSGLNSTLEP 490 500 510 520 530 540 VKDETCPVKQ PEYSAYRESS FGFGILEVKN ETHALWSWNR NQDLYYLAAD VIHIVRQPEM CSVCN
	Isoform 1 [UniParc]. Checksum: B068B6603EA100DE	FASTA	516	58,339
	10 20 30 40 50 60 MVVKYTMSMS FFVIFASTVT IIVHGFPSTL DGPLNPVTAP LDPNLNPIAF DLPESDPSFV 70 80 90 100 110 120 KPISEFLLPE QISVSLSYSF DSVWISWVTG EYQIGEKDSA PLDPNCVQSI VQYREFDVRR 130 140 150 160 170 180 TRKQNATGHS IVYNQQYSSE NGFMNYTSGI IHHVQLTGLK PNTLYRYQCG DPSLSAMSKE 190 200 210 220 230 240 YYFRTMPKST SENYPHRIVV AGDLGLTYNT STVLGHILSN HPDLVVLLGG FSYADTYLAN 250 260 270 280 290 300 KTKLDCSSCH CDQNGTSSDC GSCYSSGETY QPRWDYWGRF MEPLTANVPT MMVAGEHEIE 310 320 330 340 350 360 PQTENNLTFA AYSSRFAFPS NESADQYIWL ESDLIKINRS ETPWVVATWS LPWYSTFKGH 370 380 390 400 410 420 YREAESMRIH LEDLLYNYRV DIVFNSHVDA YERSNRVYNY TLDQCGPVYI TTGAGGAGKL 430 440 450 460 470 480 ETQHVDDPGN IPDPSQNYSC RSSGLNSTLE PVKDETCPVK QPEYSAYRES SFGFGILEVK 490 500 510 NETHALWSWN RNQDLYYLAA DVIHIVRQPE MCSVCN
	Isoform 3 [UniParc]. Checksum: 8352AE4B96D45D33	FASTA	428	48,313
	10 20 30 40 50 60 MVVVLYTTGE YQIGEKDSAP LDPNCVQSIV QYREFDVRRT RKQNATGHSI VYNQQYSSEN 70 80 90 100 110 120 GFMNYTSGII HHVQLTGLKP NTLYRYQCGD PSLSAMSKEY YFRTMPKSTS ENYPHRIVVA 130 140 150 160 170 180 GDLGLTYNTS TVLGHILSNH PDLVVLLGGF SYADTYLANK TKLDCSSCHC DQNGTSSDCG 190 200 210 220 230 240 SCYSSGETYQ PRWDYWGRFM EPLTANVPTM MVAGEHEIEP QTENNLTFAA YSSRFAFPSN 250 260 270 280 290 300 ESGSFSPLYY SFNAGGAHFI VLNSYTLYDN SSDQYIWLES DLIKINRSET PWVVATWSLP 310 320 330 340 350 360 WYSTFKGHYR EAESMRIHLE DLLYNYRVDI VFNSHVDAYE RSNRVYNYTL DQCGPVYITT 370 380 390 400 410 420 GAGGAGKLET QHVDDPGNIP DPSQNYSCRS SGLNSTLEPV KDETCPVKQP EYSAYRESSF GFGILEVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation." Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D. J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), GENE FAMILY, NOMENCLATURE. Strain: cv. Col-1.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation." Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M. Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[5]	"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower." Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D. Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF492665 mRNA. Translation: AAM15914.1. AC003974 Genomic DNA. Translation: AAC04486.1. CP002685 Genomic DNA. Translation: AEC08736.1. CP002685 Genomic DNA. Translation: AEC08737.1. CP002685 Genomic DNA. Translation: AEC08738.1. AY090894 mRNA. Translation: AAM16284.1. BX818780 mRNA. No translation available.
IPI	IPI00535972. IPI00541502. IPI00542620.
PIR	T00791.
RefSeq	NP_180836.1. NM_128837.1. NP_850198.1. NM_179867.1. NP_973585.1. NM_201856.3.
UniGene	At.38023.
3D structure databases
ProteinModelPortal	O48840.
SMR	O48840. Positions 51-537.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G32770.3; AT2G32770.3; AT2G32770.
GeneID	817838.
KEGG	ath:AT2G32770.
Organism-specific databases
TAIR	At2g32770.
Phylogenomic databases
eggNOG	NOG265012.
HOGENOM	HOG000238330.
InParanoid	Q3EBP9.
OMA	SAMSKEY.
PhylomeDB	O48840.
ProtClustDB	CLSN2913063.
Gene expression databases
Genevestigator	O48840.
Family and domain databases
Gene3D	2.60.40.380. 1 hit.
InterPro	IPR004843. Metallo_PEstase_dom. IPR008963. Purple_acid_Pase-like_N. IPR015914. Purple_acid_Pase_N. IPR025733. Purple_acid_PPase_C_dom. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. PF14008. Metallophos_C. 1 hit. [Graphical view]
SUPFAM	SSF49363. Purple_Pase_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPA13_ARATH

Accession

Primary (citable) accession number: O48840
Secondary accession number(s): Q3EBP9, Q8RX38

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	September 22, 2009
Last modified:	May 29, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program