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O48840 (PPA13_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Purple acid phosphatase 13

EC=3.1.3.2
Gene names
Name:PAP13
Synonyms:AT2
Ordered Locus Names:At2g32770
ORF Names:F24L7.9
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length545 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed in stems, leaves, flowers and siliques. Ref.5

Sequence similarities

Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.

Sequence caution

The sequence BX818780 differs from that shown. Reason: Frameshift at position 427.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   LigandIron
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdephosphorylation

Inferred from sequence or structural similarity Ref.1Ref.5. Source: GOC

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionacid phosphatase activity

Inferred from sequence or structural similarity Ref.1Ref.5. Source: TAIR

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 (identifier: O48840-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform 1 (identifier: O48840-1)

The sequence of this isoform differs from the canonical sequence as follows:
     324-353: GSFSPLYYSFNAGGAHFIVLNSYTLYDNSS → A
Note: May be due to a competing donor splice site. No experimental confirmation available.
Isoform 3 (identifier: O48840-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-88: MVVKYTMSMS...SFDSVWISWV → MVVVLYT
     509-509: K → S
     510-545: Missing.
Note: May be due to introns retention. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 545520Purple acid phosphatase 13
PRO_0000372817

Regions

Region416 – 4183Substrate binding By similarity

Sites

Active site3891Proton donor By similarity
Metal binding2031Iron By similarity
Metal binding2331Iron By similarity
Metal binding4161Zinc By similarity

Amino acid modifications

Glycosylation1251N-linked (GlcNAc...) Potential
Glycosylation1451N-linked (GlcNAc...) Potential
Glycosylation2091N-linked (GlcNAc...) Potential
Glycosylation2401N-linked (GlcNAc...) Potential
Glycosylation2541N-linked (GlcNAc...) Potential
Glycosylation3061N-linked (GlcNAc...) Potential
Glycosylation3211N-linked (GlcNAc...) Potential
Glycosylation3511N-linked (GlcNAc...) Potential
Glycosylation3671N-linked (GlcNAc...) Potential
Glycosylation4281N-linked (GlcNAc...) Potential
Glycosylation4661N-linked (GlcNAc...) Potential
Glycosylation4751N-linked (GlcNAc...) Potential
Glycosylation5101N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 8888MVVKY…WISWV → MVVVLYT in isoform 3.
VSP_037193
Alternative sequence324 – 35330GSFSP…YDNSS → A in isoform 1.
VSP_038047
Alternative sequence5091K → S in isoform 3.
VSP_037196
Alternative sequence510 – 54536Missing in isoform 3.
VSP_037197

Experimental info

Sequence conflict3841S → R in BX818780. Ref.4
Sequence conflict387 – 3882KG → TR in BX818780. Ref.4
Sequence conflict3921E → D in BX818780. Ref.4
Sequence conflict4021D → N in BX818780. Ref.4
Isoform 3:
Sequence conflict471G → D in AAM16284. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 [UniParc].

Last modified September 22, 2009. Version 2.
Checksum: 4E35C8DCDD2B293C

FASTA54561,543
        10         20         30         40         50         60 
MVVKYTMSMS FFVIFASTVT IIVHGFPSTL DGPLNPVTAP LDPNLNPIAF DLPESDPSFV 

        70         80         90        100        110        120 
KPISEFLLPE QISVSLSYSF DSVWISWVTG EYQIGEKDSA PLDPNCVQSI VQYREFDVRR 

       130        140        150        160        170        180 
TRKQNATGHS IVYNQQYSSE NGFMNYTSGI IHHVQLTGLK PNTLYRYQCG DPSLSAMSKE 

       190        200        210        220        230        240 
YYFRTMPKST SENYPHRIVV AGDLGLTYNT STVLGHILSN HPDLVVLLGG FSYADTYLAN 

       250        260        270        280        290        300 
KTKLDCSSCH CDQNGTSSDC GSCYSSGETY QPRWDYWGRF MEPLTANVPT MMVAGEHEIE 

       310        320        330        340        350        360 
PQTENNLTFA AYSSRFAFPS NESGSFSPLY YSFNAGGAHF IVLNSYTLYD NSSDQYIWLE 

       370        380        390        400        410        420 
SDLIKINRSE TPWVVATWSL PWYSTFKGHY REAESMRIHL EDLLYNYRVD IVFNSHVDAY 

       430        440        450        460        470        480 
ERSNRVYNYT LDQCGPVYIT TGAGGAGKLE TQHVDDPGNI PDPSQNYSCR SSGLNSTLEP 

       490        500        510        520        530        540 
VKDETCPVKQ PEYSAYRESS FGFGILEVKN ETHALWSWNR NQDLYYLAAD VIHIVRQPEM 


CSVCN 

« Hide

Isoform 1 [UniParc].

Checksum: B068B6603EA100DE
Show »

FASTA51658,339
Isoform 3 [UniParc].

Checksum: 8352AE4B96D45D33
Show »

FASTA42848,313

References

« Hide 'large scale' references
[1]"Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), GENE FAMILY, NOMENCLATURE.
Strain: cv. Col-1.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[5]"Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF492665 mRNA. Translation: AAM15914.1.
AC003974 Genomic DNA. Translation: AAC04486.1.
CP002685 Genomic DNA. Translation: AEC08736.1.
CP002685 Genomic DNA. Translation: AEC08737.1.
CP002685 Genomic DNA. Translation: AEC08738.1.
AY090894 mRNA. Translation: AAM16284.1.
BX818780 mRNA. No translation available.
PIRT00791.
RefSeqNP_180836.1. NM_128837.1. [O48840-1]
NP_850198.1. NM_179867.1. [O48840-3]
NP_973585.1. NM_201856.3. [O48840-2]
UniGeneAt.38023.

3D structure databases

ProteinModelPortalO48840.
SMRO48840. Positions 51-537.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEO48840.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G32770.3; AT2G32770.3; AT2G32770. [O48840-2]
GeneID817838.
KEGGath:AT2G32770.

Organism-specific databases

TAIRAT2G32770.

Phylogenomic databases

eggNOGNOG265012.
HOGENOMHOG000238330.
InParanoidQ3EBP9.
OMASAMSKEY.
PhylomeDBO48840.

Enzyme and pathway databases

BioCycARA:AT2G32770-MONOMER.
ARA:GQT-2796-MONOMER.
ARA:GQT-2797-MONOMER.

Gene expression databases

GenevestigatorO48840.

Family and domain databases

Gene3D2.60.40.380. 1 hit.
3.60.21.10. 2 hits.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 3 hits.
ProtoNetSearch...

Entry information

Entry namePPA13_ARATH
AccessionPrimary (citable) accession number: O48840
Secondary accession number(s): Q3EBP9, Q8RX38
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 22, 2009
Last modified: June 11, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names