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O48840

- PPA13_ARATH

UniProt

O48840 - PPA13_ARATH

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Protein
Purple acid phosphatase 13
Gene
PAP13, AT2, At2g32770, F24L7.9
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactori

Binds 1 iron ion per subunit By similarity.
Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi203 – 2031Iron By similarity
Metal bindingi233 – 2331Iron By similarity
Active sitei389 – 3891Proton donor By similarity
Metal bindingi416 – 4161Zinc By similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: TAIR
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT2G32770-MONOMER.
ARA:GQT-2796-MONOMER.
ARA:GQT-2797-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase 13 (EC:3.1.3.2)
Gene namesi
Name:PAP13
Synonyms:AT2
Ordered Locus Names:At2g32770
ORF Names:F24L7.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G32770.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed prediction
Add
BLAST
Chaini26 – 545520Purple acid phosphatase 13
PRO_0000372817Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi125 – 1251N-linked (GlcNAc...) Reviewed prediction
Glycosylationi145 – 1451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi209 – 2091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi240 – 2401N-linked (GlcNAc...) Reviewed prediction
Glycosylationi254 – 2541N-linked (GlcNAc...) Reviewed prediction
Glycosylationi306 – 3061N-linked (GlcNAc...) Reviewed prediction
Glycosylationi321 – 3211N-linked (GlcNAc...) Reviewed prediction
Glycosylationi351 – 3511N-linked (GlcNAc...) Reviewed prediction
Glycosylationi367 – 3671N-linked (GlcNAc...) Reviewed prediction
Glycosylationi428 – 4281N-linked (GlcNAc...) Reviewed prediction
Glycosylationi466 – 4661N-linked (GlcNAc...) Reviewed prediction
Glycosylationi475 – 4751N-linked (GlcNAc...) Reviewed prediction
Glycosylationi510 – 5101N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiO48840.

Expressioni

Tissue specificityi

Expressed in stems, leaves, flowers and siliques.1 Publication

Gene expression databases

GenevestigatoriO48840.

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliO48840.
SMRiO48840. Positions 51-537.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni416 – 4183Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG265012.
HOGENOMiHOG000238330.
InParanoidiQ3EBP9.
OMAiSAMSKEY.
PhylomeDBiO48840.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 2 hits.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 3 hits.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 2 (identifier: O48840-2) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MVVKYTMSMS FFVIFASTVT IIVHGFPSTL DGPLNPVTAP LDPNLNPIAF    50
DLPESDPSFV KPISEFLLPE QISVSLSYSF DSVWISWVTG EYQIGEKDSA 100
PLDPNCVQSI VQYREFDVRR TRKQNATGHS IVYNQQYSSE NGFMNYTSGI 150
IHHVQLTGLK PNTLYRYQCG DPSLSAMSKE YYFRTMPKST SENYPHRIVV 200
AGDLGLTYNT STVLGHILSN HPDLVVLLGG FSYADTYLAN KTKLDCSSCH 250
CDQNGTSSDC GSCYSSGETY QPRWDYWGRF MEPLTANVPT MMVAGEHEIE 300
PQTENNLTFA AYSSRFAFPS NESGSFSPLY YSFNAGGAHF IVLNSYTLYD 350
NSSDQYIWLE SDLIKINRSE TPWVVATWSL PWYSTFKGHY REAESMRIHL 400
EDLLYNYRVD IVFNSHVDAY ERSNRVYNYT LDQCGPVYIT TGAGGAGKLE 450
TQHVDDPGNI PDPSQNYSCR SSGLNSTLEP VKDETCPVKQ PEYSAYRESS 500
FGFGILEVKN ETHALWSWNR NQDLYYLAAD VIHIVRQPEM CSVCN 545

Note: No experimental confirmation available.

Length:545
Mass (Da):61,543
Last modified:September 22, 2009 - v2
Checksum:i4E35C8DCDD2B293C
GO
Isoform 1 (identifier: O48840-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     324-353: GSFSPLYYSFNAGGAHFIVLNSYTLYDNSS → A

Note: May be due to a competing donor splice site. No experimental confirmation available.

Show »
Length:516
Mass (Da):58,339
Checksum:iB068B6603EA100DE
GO
Isoform 3 (identifier: O48840-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-88: MVVKYTMSMS...SFDSVWISWV → MVVVLYT
     509-509: K → S
     510-545: Missing.

Note: May be due to introns retention. No experimental confirmation available.

Show »
Length:428
Mass (Da):48,313
Checksum:i8352AE4B96D45D33
GO

Sequence cautioni

The sequence BX818780 differs from that shown. Reason: Frameshift at position 427.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 8888MVVKY…WISWV → MVVVLYT in isoform 3.
VSP_037193Add
BLAST
Alternative sequencei324 – 35330GSFSP…YDNSS → A in isoform 1.
VSP_038047Add
BLAST
Alternative sequencei509 – 5091K → S in isoform 3.
VSP_037196
Alternative sequencei510 – 54536Missing in isoform 3.
VSP_037197Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti384 – 3841S → R in BX818780. 1 Publication
Sequence conflicti387 – 3882KG → TR in BX818780. 1 Publication
Sequence conflicti392 – 3921E → D in BX818780. 1 Publication
Sequence conflicti402 – 4021D → N in BX818780. 1 Publication
Isoform 3 (identifier: O48840-3)
Sequence conflicti47 – 471G → D in AAM16284. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF492665 mRNA. Translation: AAM15914.1.
AC003974 Genomic DNA. Translation: AAC04486.1.
CP002685 Genomic DNA. Translation: AEC08736.1.
CP002685 Genomic DNA. Translation: AEC08737.1.
CP002685 Genomic DNA. Translation: AEC08738.1.
AY090894 mRNA. Translation: AAM16284.1.
BX818780 mRNA. No translation available.
PIRiT00791.
RefSeqiNP_180836.1. NM_128837.1. [O48840-1]
NP_850198.1. NM_179867.1. [O48840-3]
NP_973585.1. NM_201856.3. [O48840-2]
UniGeneiAt.38023.

Genome annotation databases

EnsemblPlantsiAT2G32770.3; AT2G32770.3; AT2G32770. [O48840-2]
GeneIDi817838.
KEGGiath:AT2G32770.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF492665 mRNA. Translation: AAM15914.1 .
AC003974 Genomic DNA. Translation: AAC04486.1 .
CP002685 Genomic DNA. Translation: AEC08736.1 .
CP002685 Genomic DNA. Translation: AEC08737.1 .
CP002685 Genomic DNA. Translation: AEC08738.1 .
AY090894 mRNA. Translation: AAM16284.1 .
BX818780 mRNA. No translation available.
PIRi T00791.
RefSeqi NP_180836.1. NM_128837.1. [O48840-1 ]
NP_850198.1. NM_179867.1. [O48840-3 ]
NP_973585.1. NM_201856.3. [O48840-2 ]
UniGenei At.38023.

3D structure databases

ProteinModelPortali O48840.
SMRi O48840. Positions 51-537.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi O48840.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G32770.3 ; AT2G32770.3 ; AT2G32770 . [O48840-2 ]
GeneIDi 817838.
KEGGi ath:AT2G32770.

Organism-specific databases

TAIRi AT2G32770.

Phylogenomic databases

eggNOGi NOG265012.
HOGENOMi HOG000238330.
InParanoidi Q3EBP9.
OMAi SAMSKEY.
PhylomeDBi O48840.

Enzyme and pathway databases

BioCyci ARA:AT2G32770-MONOMER.
ARA:GQT-2796-MONOMER.
ARA:GQT-2797-MONOMER.

Gene expression databases

Genevestigatori O48840.

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 2 hits.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 3 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purple acid phosphatases of Arabidopsis thaliana. Comparative analysis and differential regulation by phosphate deprivation."
    Li D., Zhu H., Liu K., Liu X., Leggewie G., Udvardi M., Wang D.
    J. Biol. Chem. 277:27772-27781(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), GENE FAMILY, NOMENCLATURE.
    Strain: cv. Col-1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation."
    Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M., Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M., Weissenbach J., Salanoubat M.
    Genome Res. 14:406-413(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  5. "Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower."
    Zhu H., Qian W., Lu X., Li D., Liu X., Liu K., Wang D.
    Plant Mol. Biol. 59:581-594(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiPPA13_ARATH
AccessioniPrimary (citable) accession number: O48840
Secondary accession number(s): Q3EBP9, Q8RX38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 22, 2009
Last modified: June 11, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi