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Protein

Serine/threonine-protein kinase BIK1

Gene

BIK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role in immune responses (PubMed:20413097). Required to activate the resistance responses to necrotrophic pathogens (PubMed:16339855). Phosphorylates FLS2 and BAK1 (PubMed:20404519, PubMed:24104392). Involved in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling and defense responses downstream of FLS2. Acts additively with PBL1 in PTI defenses (PubMed:20413097). Acts as positive regulator of the PAMP flg22-induced increase of cytosolic calcium. Binds directly and phosphorylates the NADPH oxidase RBOHD at specific sites in a calcium-independent manner to enhance reactive oxygen species (ROS) generation upon flg22 perception. ROS production in response to flg22 controls stomatal movement and restriction of bacterial entry into leaf tissues (PubMed:24629339). Seems not required for flg22-induced MAPK activation (Probable). Required for Pep1-induced defenses. Pep1 is an endogenous elicitor that potentiates PAMP-inducible plant responses. In association with PEPR1, acts downstream of the canonical ethylene signaling cascade to regulate ethylene responses (PubMed:23431184). Involved in ethylene signaling. Destabilizes EIN3, the key transcription activator in ethylene signaling, and represses EIN3-dependent transcription (PubMed:26021844). Acts as a negative regulator in brassinosteroid (BR) signaling. Functions in BR signaling by direct interaction with the BR receptor BRI1 cytosolic kinase domain (PubMed:23818580).Curated8 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Enzyme regulationi

Kinase activation is repressed by the phosphatase PP2C38.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei105ATPPROSITE-ProRule annotation1
Active sitei202Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi73 – 81ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • defense response to fungus Source: TAIR
  • pathogen-associated molecular pattern dependent induction by symbiont of host innate immune response Source: TAIR
  • protein autophosphorylation Source: TAIR
  • response to fungus Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Immunity, Innate immunity, Plant defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase BIK1Curated (EC:2.7.11.11 Publication)
Alternative name(s):
Protein BOTRYTIS-INDUCED KINASE 11 Publication
Gene namesi
Name:BIK11 Publication
Ordered Locus Names:At2g39660
ORF Names:F12L6.32, F17A14.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G39660.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • nucleolus Source: TAIR
  • nucleus Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Altered growth traits, early flowering, weak stems, small siliques and reduced fertility. Mutant plants have a severely impaired resistance to Botrytis and A.brassicicola (PubMed:16339855). Reduced plant size (PubMed:20413097, PubMed:27679580). Enhanced resistance to Plasmodiophora brassicae, a soil-borne obligate pathogen that causes clubroot disease (PubMed:27679580). Hypersensitivity to the plant hormone brassinolide (PubMed:23818580).4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2G → S: Drastic reduction of plasma membrane localization and strong increase of cytoplasmic localization. 1 Publication1
Mutagenesisi105K → E: Loss of autophosphorylation. 1 Publication1
Mutagenesisi150Y → F: Loss of function in innate immunity. 1 Publication1
Mutagenesisi202D → A: Abolishes kinase activity. 1 Publication1
Mutagenesisi243Y → F: Loss of function in innate immunity. 1 Publication1
Mutagenesisi250Y → F: Loss of function in innate immunity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCurated
ChainiPRO_00003111182 – 395Serine/threonine-protein kinase BIK1Add BLAST394

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Lipidationi4S-palmitoyl cysteineBy similarity1
Modified residuei48Phosphoserine; by autocatalysis and BAK11 Publication1
Modified residuei54Phosphoserine; by autocatalysis1 Publication1
Modified residuei56Phosphothreonine; by autocatalysis1 Publication1
Modified residuei71Phosphoserine; by autocatalysis and BAK11 Publication1
Modified residuei129Phosphoserine; by autocatalysis1 Publication1
Modified residuei150Phosphotyrosine1 Publication1
Modified residuei168Phosphotyrosine; by autocatalysis1 Publication1
Modified residuei206Phosphoserine; by autocatalysis and BAK11 Publication1
Modified residuei214Phosphotyrosine; by autocatalysis1 Publication1
Modified residuei233Phosphoserine; by autocatalysis2 Publications1
Modified residuei236Phosphoserine; by autocatalysis and BAK13 Publications1
Modified residuei237Phosphothreonine; by autocatalysis and BAK13 Publications1
Modified residuei242Phosphothreonine; by autocatalysis and BAK11 Publication1
Modified residuei243Phosphotyrosine1 Publication1
Modified residuei250Phosphotyrosine; by autocatalysis2 Publications1
Modified residuei252Phosphoserine; by autocatalysis1 Publication1
Modified residuei253Phosphoserine; by autocatalysis1 Publication1
Modified residuei314Phosphothreonine; by autocatalysis1 Publication1
Modified residuei360Phosphoserine; by autocatalysis and BAK11 Publication1
Modified residuei362Phosphothreonine; by autocatalysis and BAK11 Publication1
Modified residuei368Phosphothreonine; by autocatalysis and BAK11 Publication1

Post-translational modificationi

Phosphorylated by FLS2 and BAK1 (PubMed:20404519). Autophosphorylated (PubMed:22504181, PubMed:23431184, PubMed:24104392, PubMed:26021844). Autophosphorylation is reduced in presence of the Xanthomonas campestris effector XopAC/AvrAC (PubMed:22504181). Phosphorylated in response to the microbe-associated molecular pattern (MAMP) flg22 (PubMed:20413097, PubMed:22504181). Phosphorylation in response to flg22 is abolished in presence of the Xanthomonas campestris effector XopAC/AvrAC (PubMed:22504181). Phosphorylated at Ser-233, Ser-236 and THR-237 by PEPR1 (PubMed:23431184). Phosphorylated at Tyr-150, Tyr-243 and Tyr-250. Tyrosine phosphorylation is required for BIK1 function in plant innate immunity (PubMed:24532660).7 Publications

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiO48814.
PRIDEiO48814.

PTM databases

iPTMnetiO48814.

Expressioni

Inductioni

By infection with necrotrophic pathogens and by paraquat. Not induced by salicylic acid, jasmonate or 1-aminocyclopropane-1-carboxylate (ACC) (PubMed:16339855). Induced by flagellin (flg22) (PubMed:20413097).2 Publications

Gene expression databases

GenevisibleiO48814. AT.

Interactioni

Subunit structurei

Interacts with FLS2 (PubMed:20413097, PubMed:20404519). Activation of FLS2 by flagellin (flg22) induces the dissociation of the complex (PubMed:20413097). Interacts with BAK1 (PubMed:20404519). Interacts with the Xanthomonas campestris effector XopAC/AvrAC (PubMed:22504181). Interacts with CPK28 (PubMed:25525792). Interacts with PEPR1 (PubMed:23431184). Interacts with PP2C38 (PubMed:27494702). Interacts with BRI1 (PubMed:23818580). Interacts with RBOHD (PubMed:24629339).8 Publications

Protein-protein interaction databases

BioGridi3887. 6 interactors.
DIPiDIP-51510N.
IntActiO48814. 2 interactors.
STRINGi3702.AT2G39660.1.

Structurei

3D structure databases

ProteinModelPortaliO48814.
SMRiO48814.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini67 – 356Protein kinasePROSITE-ProRule annotationAdd BLAST290

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiO48814.
OMAiHNGKSSD.
OrthoDBiEOG09360DFG.
PhylomeDBiO48814.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O48814-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSCFSSRVK ADIFHNGKSS DLYGLSLSSR KSSSTVAAAQ KTEGEILSST
60 70 80 90 100
PVKSFTFNEL KLATRNFRPD SVIGEGGFGC VFKGWLDEST LTPTKPGTGL
110 120 130 140 150
VIAVKKLNQE GFQGHREWLT EINYLGQLSH PNLVKLIGYC LEDEHRLLVY
160 170 180 190 200
EFMQKGSLEN HLFRRGAYFK PLPWFLRVNV ALDAAKGLAF LHSDPVKVIY
210 220 230 240 250
RDIKASNILL DADYNAKLSD FGLARDGPMG DLSYVSTRVM GTYGYAAPEY
260 270 280 290 300
MSSGHLNARS DVYSFGVLLL EILSGKRALD HNRPAKEENL VDWARPYLTS
310 320 330 340 350
KRKVLLIVDN RLDTQYLPEE AVRMASVAVQ CLSFEPKSRP TMDQVVRALQ
360 370 380 390
QLQDNLGKPS QTNPVKDTKK LGFKTGTTKS SEKRFTQKPF GRHLV
Length:395
Mass (Da):44,097
Last modified:June 1, 1998 - v1
Checksum:i4C06B1FD01889440
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003674 Genomic DNA. Translation: AAB97121.1.
AC004218 Genomic DNA. Translation: AAM14921.1.
CP002685 Genomic DNA. Translation: AEC09703.1.
AF325086 mRNA. Translation: AAK17154.1.
AY062493 mRNA. Translation: AAL32571.1.
AY065029 mRNA. Translation: AAL57667.1.
AY093278 mRNA. Translation: AAM13277.1.
AY093997 mRNA. Translation: AAM16258.1.
PIRiT00574.
RefSeqiNP_181496.1. NM_129522.5.
UniGeneiAt.21254.

Genome annotation databases

EnsemblPlantsiAT2G39660.1; AT2G39660.1; AT2G39660.
GeneIDi818549.
GrameneiAT2G39660.1; AT2G39660.1; AT2G39660.
KEGGiath:AT2G39660.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003674 Genomic DNA. Translation: AAB97121.1.
AC004218 Genomic DNA. Translation: AAM14921.1.
CP002685 Genomic DNA. Translation: AEC09703.1.
AF325086 mRNA. Translation: AAK17154.1.
AY062493 mRNA. Translation: AAL32571.1.
AY065029 mRNA. Translation: AAL57667.1.
AY093278 mRNA. Translation: AAM13277.1.
AY093997 mRNA. Translation: AAM16258.1.
PIRiT00574.
RefSeqiNP_181496.1. NM_129522.5.
UniGeneiAt.21254.

3D structure databases

ProteinModelPortaliO48814.
SMRiO48814.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3887. 6 interactors.
DIPiDIP-51510N.
IntActiO48814. 2 interactors.
STRINGi3702.AT2G39660.1.

PTM databases

iPTMnetiO48814.

Proteomic databases

PaxDbiO48814.
PRIDEiO48814.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G39660.1; AT2G39660.1; AT2G39660.
GeneIDi818549.
GrameneiAT2G39660.1; AT2G39660.1; AT2G39660.
KEGGiath:AT2G39660.

Organism-specific databases

TAIRiAT2G39660.

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiO48814.
OMAiHNGKSSD.
OrthoDBiEOG09360DFG.
PhylomeDBiO48814.

Miscellaneous databases

PROiO48814.

Gene expression databases

GenevisibleiO48814. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBIK1_ARATH
AccessioniPrimary (citable) accession number: O48814
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The association with FLS2 and BAK1 is reduced after flagellin perception, BIK1 being probably released from the receptor complex upon phosphorylation.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.