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Protein

Serine/threonine-protein kinase BIK1

Gene

BIK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required to activate the resistance responses to necrotrophic pathogens. Phosphorylates FLS2 and BAK1.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051ATPPROSITE-ProRule annotation
Active sitei202 – 2021Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 819ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • defense response to fungus Source: TAIR
  • pathogen-associated molecular pattern dependent induction by symbiont of host innate immune response Source: TAIR
  • protein autophosphorylation Source: TAIR
  • response to fungus Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G39660-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase BIK1 (EC:2.7.11.1)
Alternative name(s):
Protein BOTRYTIS-INDUCED KINASE 1
Gene namesi
Name:BIK1
Ordered Locus Names:At2g39660
ORF Names:F12L6.32, F17A14.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G39660.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • nucleolus Source: TAIR
  • nucleus Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Plants show some altered growth traits and a severely impaired resistance to Botrytis and A.brassicicola.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 395394Serine/threonine-protein kinase BIK1PRO_0000311118Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis
Lipidationi4 – 41S-palmitoyl cysteineSequence analysis
Modified residuei236 – 2361PhosphoserineBy similarity
Modified residuei237 – 2371Phosphothreonine1 Publication

Post-translational modificationi

Phosphorylated by FLS2 and BAK1.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiO48814.
PRIDEiO48814.

PTM databases

iPTMnetiO48814.

Expressioni

Inductioni

By infection with necrotrophic pathogens and by paraquat. Not induced by salicylic acid, jasmonate or 1-aminocyclopropane-1-carboxylate (ACC).1 Publication

Gene expression databases

GenevisibleiO48814. AT.

Interactioni

Subunit structurei

Interacts with FLS2 and BAK1.1 Publication

Protein-protein interaction databases

BioGridi3887. 6 interactions.
DIPiDIP-51510N.
IntActiO48814. 2 interactions.
STRINGi3702.AT2G39660.1.

Structurei

3D structure databases

ProteinModelPortaliO48814.
SMRiO48814. Positions 7-351.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini67 – 356290Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiO48814.
OMAiHNGKSSD.
PhylomeDBiO48814.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O48814-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSCFSSRVK ADIFHNGKSS DLYGLSLSSR KSSSTVAAAQ KTEGEILSST
60 70 80 90 100
PVKSFTFNEL KLATRNFRPD SVIGEGGFGC VFKGWLDEST LTPTKPGTGL
110 120 130 140 150
VIAVKKLNQE GFQGHREWLT EINYLGQLSH PNLVKLIGYC LEDEHRLLVY
160 170 180 190 200
EFMQKGSLEN HLFRRGAYFK PLPWFLRVNV ALDAAKGLAF LHSDPVKVIY
210 220 230 240 250
RDIKASNILL DADYNAKLSD FGLARDGPMG DLSYVSTRVM GTYGYAAPEY
260 270 280 290 300
MSSGHLNARS DVYSFGVLLL EILSGKRALD HNRPAKEENL VDWARPYLTS
310 320 330 340 350
KRKVLLIVDN RLDTQYLPEE AVRMASVAVQ CLSFEPKSRP TMDQVVRALQ
360 370 380 390
QLQDNLGKPS QTNPVKDTKK LGFKTGTTKS SEKRFTQKPF GRHLV
Length:395
Mass (Da):44,097
Last modified:June 1, 1998 - v1
Checksum:i4C06B1FD01889440
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003674 Genomic DNA. Translation: AAB97121.1.
AC004218 Genomic DNA. Translation: AAM14921.1.
CP002685 Genomic DNA. Translation: AEC09703.1.
AF325086 mRNA. Translation: AAK17154.1.
AY062493 mRNA. Translation: AAL32571.1.
AY065029 mRNA. Translation: AAL57667.1.
AY093278 mRNA. Translation: AAM13277.1.
AY093997 mRNA. Translation: AAM16258.1.
PIRiT00574.
RefSeqiNP_181496.1. NM_129522.4.
UniGeneiAt.21254.

Genome annotation databases

EnsemblPlantsiAT2G39660.1; AT2G39660.1; AT2G39660.
GeneIDi818549.
GrameneiAT2G39660.1; AT2G39660.1; AT2G39660.
KEGGiath:AT2G39660.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003674 Genomic DNA. Translation: AAB97121.1.
AC004218 Genomic DNA. Translation: AAM14921.1.
CP002685 Genomic DNA. Translation: AEC09703.1.
AF325086 mRNA. Translation: AAK17154.1.
AY062493 mRNA. Translation: AAL32571.1.
AY065029 mRNA. Translation: AAL57667.1.
AY093278 mRNA. Translation: AAM13277.1.
AY093997 mRNA. Translation: AAM16258.1.
PIRiT00574.
RefSeqiNP_181496.1. NM_129522.4.
UniGeneiAt.21254.

3D structure databases

ProteinModelPortaliO48814.
SMRiO48814. Positions 7-351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3887. 6 interactions.
DIPiDIP-51510N.
IntActiO48814. 2 interactions.
STRINGi3702.AT2G39660.1.

PTM databases

iPTMnetiO48814.

Proteomic databases

PaxDbiO48814.
PRIDEiO48814.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G39660.1; AT2G39660.1; AT2G39660.
GeneIDi818549.
GrameneiAT2G39660.1; AT2G39660.1; AT2G39660.
KEGGiath:AT2G39660.

Organism-specific databases

TAIRiAT2G39660.

Phylogenomic databases

eggNOGiKOG1187. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiO48814.
OMAiHNGKSSD.
PhylomeDBiO48814.

Enzyme and pathway databases

BioCyciARA:AT2G39660-MONOMER.

Miscellaneous databases

PROiO48814.

Gene expression databases

GenevisibleiO48814. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "The membrane-anchored BOTRYTIS-INDUCED KINASE1 plays distinct roles in Arabidopsis resistance to necrotrophic and biotrophic pathogens."
    Veronese P., Nakagami H., Bluhm B., AbuQamar S., Chen X., Salmeron J., Dietrich R.A., Hirt H., Mengiste T.
    Plant Cell 18:257-273(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION, DISRUPTION PHENOTYPE.
  5. "Phosphorylation of receptor-like cytoplasmic kinases by bacterial flagellin."
    Lu D., Wu S., He P., Shan L.
    Plant Signal. Behav. 5:598-600(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH FLS2 AND BAK1, AUTOPHOSPHORYLATION, PHOSPHORYLATION AT THR-237.

Entry informationi

Entry nameiBIK1_ARATH
AccessioniPrimary (citable) accession number: O48814
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: June 1, 1998
Last modified: May 11, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The association with FLS2 and BAK1 is reduced after flagellin perception, BIK1 being probably released from the receptor complex upon phosphorylation.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.