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Protein

Cytochrome P450 734A1

Gene

CYP734A1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochrome P450 involved in brassinosteroids (BRs) inactivation and regulation of BRs homeostasis. Inactivates the BRs castasterone and brassinolide through carbon 26 hydroxylation. Acts in association with CYP72C1 to inactivate BRs and modulate photomorphogenesis.5 Publications

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi463Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

  • brassinosteroid homeostasis Source: TAIR
  • brassinosteroid metabolic process Source: TAIR
  • regulation of growth Source: UniProtKB-KW
  • response to brassinosteroid Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Growth regulation

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-ATH-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-ATH-211976. Endogenous sterols.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 734A1 (EC:1.14.-.-)
Alternative name(s):
Protein PHYB ACTIVATION-TAGGED SUPPRESSOR 1
Gene namesi
Name:CYP734A1
Synonyms:BAS1
Ordered Locus Names:At2g26710
ORF Names:F18A8.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G26710.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei13 – 33HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth condition, but reduced responsiveness of hypocotyls to light and increased responsiveness to brassinolide. Strong reduction of C-26 hydroxylase activity.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004111951 – 520Cytochrome P450 734A1Add BLAST520

Proteomic databases

PaxDbiO48786.

Expressioni

Inductioni

By brassinolide, auxin and dark treatment.2 Publications

Gene expression databases

GenevisibleiO48786. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G26710.1.

Structurei

3D structure databases

ProteinModelPortaliO48786.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000039125.
InParanoidiO48786.
KOiK15639.
OMAiPRAAKHP.
OrthoDBiEOG0936073R.
PhylomeDBiO48786.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O48786-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEESSSWFI PKVLVLSVIL SLVIVKGMSL LWWRPRKIEE HFSKQGIRGP
60 70 80 90 100
PYHFFIGNVK ELVGMMLKAS SHPMPFSHNI LPRVLSFYHH WRKIYGATFL
110 120 130 140 150
VWFGPTFRLT VADPDLIREI FSKSEFYEKN EAHPLVKQLE GDGLLSLKGE
160 170 180 190 200
KWAHHRKIIS PTFHMENLKL LVPVVLKSVT DMVDKWSDKL SENGEVEVDV
210 220 230 240 250
YEWFQILTED VISRTAFGSS YEDGRAVFRL QAQQMLLCAE AFQKVFIPGY
260 270 280 290 300
RFFPTRGNLK SWKLDKEIRK SLLKLIERRR QNAIDGEGEE CKEPAAKDLL
310 320 330 340 350
GLMIQAKNVT VQDIVEECKS FFFAGKQTTS NLLTWTTILL SMHPEWQAKA
360 370 380 390 400
RDEVLRVCGS RDVPTKDHVV KLKTLSMILN ESLRLYPPIV ATIRRAKSDV
410 420 430 440 450
KLGGYKIPCG TELLIPIIAV HHDQAIWGND VNEFNPARFA DGVPRAAKHP
460 470 480 490 500
VGFIPFGLGV RTCIGQNLAI LQAKLTLAVM IQRFTFHLAP TYQHAPTVLM
510 520
LLYPQHGAPI TFRRLTNHED
Length:520
Mass (Da):59,490
Last modified:June 1, 1998 - v1
Checksum:i5B51EB6A0CC0FA59
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003105 Genomic DNA. Translation: AAB95305.1.
CP002685 Genomic DNA. Translation: AEC07878.1.
BT010564 mRNA. Translation: AAQ65187.1.
AK175232 mRNA. Translation: BAD42995.1.
AK229743 mRNA. Translation: BAF01580.1.
PIRiH84663.
RefSeqiNP_180239.1. NM_128228.4.
UniGeneiAt.43115.

Genome annotation databases

EnsemblPlantsiAT2G26710.1; AT2G26710.1; AT2G26710.
GeneIDi817212.
GrameneiAT2G26710.1; AT2G26710.1; AT2G26710.
KEGGiath:AT2G26710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003105 Genomic DNA. Translation: AAB95305.1.
CP002685 Genomic DNA. Translation: AEC07878.1.
BT010564 mRNA. Translation: AAQ65187.1.
AK175232 mRNA. Translation: BAD42995.1.
AK229743 mRNA. Translation: BAF01580.1.
PIRiH84663.
RefSeqiNP_180239.1. NM_128228.4.
UniGeneiAt.43115.

3D structure databases

ProteinModelPortaliO48786.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G26710.1.

Proteomic databases

PaxDbiO48786.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G26710.1; AT2G26710.1; AT2G26710.
GeneIDi817212.
GrameneiAT2G26710.1; AT2G26710.1; AT2G26710.
KEGGiath:AT2G26710.

Organism-specific databases

TAIRiAT2G26710.

Phylogenomic databases

eggNOGiKOG0157. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000039125.
InParanoidiO48786.
KOiK15639.
OMAiPRAAKHP.
OrthoDBiEOG0936073R.
PhylomeDBiO48786.

Enzyme and pathway databases

ReactomeiR-ATH-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-ATH-211976. Endogenous sterols.

Miscellaneous databases

PROiO48786.

Gene expression databases

GenevisibleiO48786. AT.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC734A_ARATH
AccessioniPrimary (citable) accession number: O48786
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2011
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants overexpressing CYP734A1 exhibit severe dwarfism.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.