O48780 (KCS11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-ketoacyl-CoA synthase 11
Short name=KCS-11
EC=2.3.1.-
EC=2.3.1.119

Alternative name(s):
Very long-chain fatty acid condensing enzyme 11
Short name=VLCFA condensing enzyme 11

Gene names

Name:	KCS11
Ordered Locus Names:	At2g26640
ORF Names:	F18A8.1

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Active on both saturated and mono-unsaturated acyl chains C16 to C20. Ref.5
Catalytic activity	Stearoyl-CoA + malonyl-CoA + 2 NAD(P)H = icosanoyl-CoA + CO2 + CoA + 2 NAD(P)+ + H2O.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Only expressed in guard cells.
Induction	Repressed by herbicides such as flufenacet and benfuresate. Ref.4
Sequence similarities	Belongs to the chalcone/stilbene synthases family. Contains 1 FAE (fatty acid elongase) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD NADP
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: InterPro response to cold Inferred from expression pattern. Source: TAIR response to light stimulus Inferred from expression pattern. Source: TAIR
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	icosanoyl-CoA synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	3-ketoacyl-CoA synthase 11		PRO_0000249103
Regions
	Transmembrane	36 – 56	21	Helical; Potential
	Transmembrane	75 – 95	21	Helical; Potential
	Domain	92 – 381	290	FAE
Sites
	Active site	236	1	By similarity
	Active site	315	1	By similarity
	Active site	399	1	By similarity
	Active site	403	1	By similarity
	Active site	436	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O48780 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: DB922BCB2B33662F	FASTA	509	57,815
	10 20 30 40 50 60 MDVEQKKPLI ESSDRNLPDF KKSVKLKYVK LGYHYLITHG MYLFLSPLVL VIAAQISTFS 70 80 90 100 110 120 VTDLRSLWEH LQYNLISVVV CSMLLVFLMT IYFMTRPRPV YLVNFSCFKP DESRKCTKKI 130 140 150 160 170 180 FMDRSKLTGS FTEENLEFQR KILQRSGLGE STYLPEAVLN VPPNPCMKEA RKEAETVMFG 190 200 210 220 230 240 AIDELLAKTN VNPKDIGILI VNCSLFNPTP SLSAMVVNHY KLRGNILSYN LGGMGCSAGL 250 260 270 280 290 300 ISIDLAKHLL HSIPNTYAMV ISMENITLNW YFGNDRSKLV SNCLFRMGGA AILLSNKRWD 310 320 330 340 350 360 RRRSKYELVD TVRTHKGADD KCFGCITQEE DSASKIGVTL SKELMAVAGD ALKTNITTLG 370 380 390 400 410 420 PLVLPTSEQL LFFATLVGRK LFKMKIKPYI PDFKLAFEHF CIHAGGRAVL DELEKNLKLT 430 440 450 460 470 480 EWHMEPSRMT LYRFGNTSSS SLWYELAYSE AKGRIKKGDR IWQIAFGSGF KCNSSVWRAV 490 500 RSVNPKKEKN PWMDEIHEFP VEVPKVSTI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Flufenacet herbicide treatment phenocopies the fiddlehead mutant in Arabidopsis thaliana." Lechelt-Kunze C., Meissner R.C., Drewes M., Tietjen K. Pest Manag. Sci. 59:847-856(2003) [PubMed: 12916765] [Abstract] Cited for: INDUCTION, GENE FAMILY.
[5]	"Substrate specificity of Arabidopsis 3-ketoacyl-CoA synthases." Blacklock B.J., Jaworski J.G. Biochem. Biophys. Res. Commun. 346:583-590(2006) [PubMed: 16765910] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC003105 Genomic DNA. Translation: AAB95298.1. CP002685 Genomic DNA. Translation: AEC07869.1. BT003854 mRNA. Translation: AAO41904.1. BT005692 mRNA. Translation: AAO64112.1.
IPI	IPI00538814.
PIR	A84663.
RefSeq	NP_180232.1. NM_128221.5.
UniGene	At.48521. At.68413.
3D structure databases
ProteinModelPortal	O48780.
ModBase	Search...
Protein-protein interaction databases
IntAct	O48780. 1 interaction.
STRING	O48780.
Proteomic databases
PRIDE	O48780.
ProMEX	O48780.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G26640.1; AT2G26640.1; AT2G26640.
GeneID	817205.
GenomeReviews	Gene locus AT2G26640 in contig CT485783_GR.
KEGG	ath:AT2G26640.
NMPDR	fig|3702.1.peg.9686.
Organism-specific databases
TAIR	At2g26640.
Phylogenomic databases
eggNOG	euNOG04069.
GeneTree	EPGT00070000027963.
HOGENOM	HBG594284.
InParanoid	O48780.
OMA	ESRKCTK.
PhylomeDB	O48780.
ProtClustDB	PLN02192.
Gene expression databases
ArrayExpress	O48780.
Genevestigator	O48780.
GermOnline	AT2G26640. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR012392. 3-ktacl-CoA_syn. IPR013747. ACP_syn_III_C. IPR013601. FAE1_typ3_polyketide_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
KO	K15397.
Pfam	PF08541. ACP_syn_III_C. 1 hit. PF08392. FAE1_CUT1_RppA. 1 hit. [Graphical view]
PIRSF	PIRSF036417. 3-ktacl-CoA_syn. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
ProtoNet	Search...

Entry information

Entry name

KCS11_ARATH

Accession

Primary (citable) accession number: O48780

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	June 1, 1998
Last modified:	December 14, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program