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O48779

- BGL33_ARATH

UniProt

O48779 - BGL33_ARATH

Protein

Beta-glucosidase 33

Gene

BGLU33

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei113 – 1131SubstrateBy similarity
    Binding sitei217 – 2171SubstrateBy similarity
    Binding sitei262 – 2621SubstrateBy similarity
    Active sitei263 – 2631Proton donorBy similarity
    Binding sitei407 – 4071SubstrateBy similarity
    Active sitei479 – 4791NucleophileBy similarity
    Binding sitei529 – 5291SubstrateBy similarity

    GO - Molecular functioni

    1. beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciARA:AT2G32860-MONOMER.
    ARA:GQT-633-MONOMER.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucosidase 33 (EC:3.2.1.21)
    Short name:
    AtBGLU33
    Gene namesi
    Name:BGLU33
    Ordered Locus Names:At2g32860
    ORF Names:T21L14.20
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

    Organism-specific databases

    TAIRiAT2G32860.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 614588Beta-glucosidase 33PRO_0000389595Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi282 ↔ 290By similarity
    Glycosylationi344 – 3441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi419 – 4191N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi432 – 4321N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi491 – 4911N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO48779.
    PRIDEiO48779.

    Expressioni

    Gene expression databases

    GenevestigatoriO48779.

    Structurei

    3D structure databases

    ProteinModelPortaliO48779.
    SMRiO48779. Positions 94-567.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni536 – 5372Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088630.
    InParanoidiO48779.
    KOiK01188.
    OMAiNPQLDYD.
    PhylomeDBiO48779.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O48779-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MATATLTLFL GLLALTSTIL SFNADARPQP SDEDLGTIIG PHQTSFDDEI    50
    GIVIGPHATV DDEDIDMDMG TTVGPQTNLN DDDLGTIIGP EFEIHKQDFP 100
    ADFIFGTSVS AYQVEGAKKG SGRGLTSWDE FTHMFPEKVQ QNGDGDEGVD 150
    FYTRYKDDIK LMKELNTNGF RFSISWTRIL PYGTIKKGVN EEGVKFYNDL 200
    INELLANGIQ PSVTLFHWES PLALEMEYGG FLNERIVEDF REFANFCFKE 250
    FGDRVKNWAT FNEPSVYSVA GYSKGKKAPG RCSKWQAPKC PTGDSSEEPY 300
    IVAHNQILAH LAAVDEFRNC KKVEGGGKIG IVLVSHWFEP KDPNSSEDVK 350
    AARRSLEYQL GWFLRPLTYG QYPAEMLEDV NIRLREFTPE ESEKLRKSLD 400
    FVGLNYYGAF FSTPLAKVNS SQLNYETDLR VNWTVITNNL SLPDLQTTSM 450
    GIVIYPAGLK NILKHIKDEY MDPEIYIMEN GMDEIDYGTK NITEATNDYG 500
    RKEFIKSHIL IMGKSIRMDK VRLKGYYIWS LMDNFEWDKG YKVRFGLYYV 550
    DYNDNMKRYI RSSGKWLSEF LDSKETLHKC YFEGHREKGY APKLFDVEYL 600
    EPENSQLSYR SDFM 614

    Note: Derived from EST data. No experimental confirmation available.

    Length:614
    Mass (Da):70,190
    Last modified:June 1, 1998 - v1
    Checksum:iD1ACCE2438151D29
    GO
    Isoform 2 (identifier: O48779-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         323-324: VE → CQE
         435-446: VITNNLSLPDLQ → DSQNNSPHLK

    Show »
    Length:613
    Mass (Da):70,135
    Checksum:iE43E3BC6BE8579EF
    GO

    Sequence cautioni

    The sequence AAN60253.1 differs from that shown. Reason: Frameshift at position 548.
    Isoform 2 : The sequence AAN60253.1 differs from that shown. Reason: Frameshift at position 548.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei323 – 3242VE → CQE in isoform 2. 1 PublicationVSP_038464
    Alternative sequencei435 – 44612VITNN…LPDLQ → DSQNNSPHLK in isoform 2. 1 PublicationVSP_038465Add
    BLAST

    Sequence conflict

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti291 – 2911P → S in AAN60253. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC003033 Genomic DNA. Translation: AAB91979.1.
    CP002685 Genomic DNA. Translation: AEC08753.1.
    CP002685 Genomic DNA. Translation: AEC08754.1.
    AF083694 mRNA. Translation: AAN60253.1. Frameshift.
    PIRiT01121.
    RefSeqiNP_180845.2. NM_128846.3. [O48779-2]
    NP_973587.1. NM_201858.1. [O48779-1]
    UniGeneiAt.38011.

    Genome annotation databases

    EnsemblPlantsiAT2G32860.2; AT2G32860.2; AT2G32860. [O48779-1]
    GeneIDi817847.
    KEGGiath:AT2G32860.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC003033 Genomic DNA. Translation: AAB91979.1 .
    CP002685 Genomic DNA. Translation: AEC08753.1 .
    CP002685 Genomic DNA. Translation: AEC08754.1 .
    AF083694 mRNA. Translation: AAN60253.1 . Frameshift.
    PIRi T01121.
    RefSeqi NP_180845.2. NM_128846.3. [O48779-2 ]
    NP_973587.1. NM_201858.1. [O48779-1 ]
    UniGenei At.38011.

    3D structure databases

    ProteinModelPortali O48779.
    SMRi O48779. Positions 94-567.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PaxDbi O48779.
    PRIDEi O48779.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G32860.2 ; AT2G32860.2 ; AT2G32860 . [O48779-1 ]
    GeneIDi 817847.
    KEGGi ath:AT2G32860.

    Organism-specific databases

    TAIRi AT2G32860.

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088630.
    InParanoidi O48779.
    KOi K01188.
    OMAi NPQLDYD.
    PhylomeDBi O48779.

    Enzyme and pathway databases

    BioCyci ARA:AT2G32860-MONOMER.
    ARA:GQT-633-MONOMER.

    Gene expression databases

    Genevestigatori O48779.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Signal peptide selection derived cDNAs from Arabidopsis thaliana leaves and guard cells."
      Stracke R., Palme K.
      Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 134-614 (ISOFORM 2).
    4. Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiBGL33_ARATH
    AccessioniPrimary (citable) accession number: O48779
    Secondary accession number(s): Q8H7F9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 24, 2009
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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