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O48779

- BGL33_ARATH

UniProt

O48779 - BGL33_ARATH

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Protein

Beta-glucosidase 33

Gene

BGLU33

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei113 – 1131SubstrateBy similarity
Binding sitei217 – 2171SubstrateBy similarity
Binding sitei262 – 2621SubstrateBy similarity
Active sitei263 – 2631Proton donorBy similarity
Binding sitei407 – 4071SubstrateBy similarity
Active sitei479 – 4791NucleophileBy similarity
Binding sitei529 – 5291SubstrateBy similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT2G32860-MONOMER.
ARA:GQT-633-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 33 (EC:3.2.1.21)
Short name:
AtBGLU33
Gene namesi
Name:BGLU33
Ordered Locus Names:At2g32860
ORF Names:T21L14.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G32860.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 614588Beta-glucosidase 33PRO_0000389595Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi282 ↔ 290By similarity
Glycosylationi344 – 3441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi419 – 4191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi432 – 4321N-linked (GlcNAc...)Sequence Analysis
Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence Analysis
Glycosylationi491 – 4911N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO48779.
PRIDEiO48779.

Expressioni

Gene expression databases

GenevestigatoriO48779.

Structurei

3D structure databases

ProteinModelPortaliO48779.
SMRiO48779. Positions 94-567.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni536 – 5372Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiO48779.
KOiK01188.
OMAiNPQLDYD.
PhylomeDBiO48779.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O48779-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATATLTLFL GLLALTSTIL SFNADARPQP SDEDLGTIIG PHQTSFDDEI
60 70 80 90 100
GIVIGPHATV DDEDIDMDMG TTVGPQTNLN DDDLGTIIGP EFEIHKQDFP
110 120 130 140 150
ADFIFGTSVS AYQVEGAKKG SGRGLTSWDE FTHMFPEKVQ QNGDGDEGVD
160 170 180 190 200
FYTRYKDDIK LMKELNTNGF RFSISWTRIL PYGTIKKGVN EEGVKFYNDL
210 220 230 240 250
INELLANGIQ PSVTLFHWES PLALEMEYGG FLNERIVEDF REFANFCFKE
260 270 280 290 300
FGDRVKNWAT FNEPSVYSVA GYSKGKKAPG RCSKWQAPKC PTGDSSEEPY
310 320 330 340 350
IVAHNQILAH LAAVDEFRNC KKVEGGGKIG IVLVSHWFEP KDPNSSEDVK
360 370 380 390 400
AARRSLEYQL GWFLRPLTYG QYPAEMLEDV NIRLREFTPE ESEKLRKSLD
410 420 430 440 450
FVGLNYYGAF FSTPLAKVNS SQLNYETDLR VNWTVITNNL SLPDLQTTSM
460 470 480 490 500
GIVIYPAGLK NILKHIKDEY MDPEIYIMEN GMDEIDYGTK NITEATNDYG
510 520 530 540 550
RKEFIKSHIL IMGKSIRMDK VRLKGYYIWS LMDNFEWDKG YKVRFGLYYV
560 570 580 590 600
DYNDNMKRYI RSSGKWLSEF LDSKETLHKC YFEGHREKGY APKLFDVEYL
610
EPENSQLSYR SDFM

Note: Derived from EST data. No experimental confirmation available.

Length:614
Mass (Da):70,190
Last modified:June 1, 1998 - v1
Checksum:iD1ACCE2438151D29
GO
Isoform 2 (identifier: O48779-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     323-324: VE → CQE
     435-446: VITNNLSLPDLQ → DSQNNSPHLK

Show »
Length:613
Mass (Da):70,135
Checksum:iE43E3BC6BE8579EF
GO

Sequence cautioni

The sequence AAN60253.1 differs from that shown. Reason: Frameshift at position 548. Curated
Isoform 2 : The sequence AAN60253.1 differs from that shown. Reason: Frameshift at position 548. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti291 – 2911P → S in AAN60253. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei323 – 3242VE → CQE in isoform 2. 1 PublicationVSP_038464
Alternative sequencei435 – 44612VITNN…LPDLQ → DSQNNSPHLK in isoform 2. 1 PublicationVSP_038465Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003033 Genomic DNA. Translation: AAB91979.1.
CP002685 Genomic DNA. Translation: AEC08753.1.
CP002685 Genomic DNA. Translation: AEC08754.1.
AF083694 mRNA. Translation: AAN60253.1. Frameshift.
PIRiT01121.
RefSeqiNP_180845.2. NM_128846.3. [O48779-2]
NP_973587.1. NM_201858.1. [O48779-1]
UniGeneiAt.38011.

Genome annotation databases

EnsemblPlantsiAT2G32860.2; AT2G32860.2; AT2G32860. [O48779-1]
GeneIDi817847.
KEGGiath:AT2G32860.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003033 Genomic DNA. Translation: AAB91979.1 .
CP002685 Genomic DNA. Translation: AEC08753.1 .
CP002685 Genomic DNA. Translation: AEC08754.1 .
AF083694 mRNA. Translation: AAN60253.1 . Frameshift.
PIRi T01121.
RefSeqi NP_180845.2. NM_128846.3. [O48779-2 ]
NP_973587.1. NM_201858.1. [O48779-1 ]
UniGenei At.38011.

3D structure databases

ProteinModelPortali O48779.
SMRi O48779. Positions 94-567.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbi O48779.
PRIDEi O48779.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G32860.2 ; AT2G32860.2 ; AT2G32860 . [O48779-1 ]
GeneIDi 817847.
KEGGi ath:AT2G32860.

Organism-specific databases

TAIRi AT2G32860.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088630.
InParanoidi O48779.
KOi K01188.
OMAi NPQLDYD.
PhylomeDBi O48779.

Enzyme and pathway databases

BioCyci ARA:AT2G32860-MONOMER.
ARA:GQT-633-MONOMER.

Gene expression databases

Genevestigatori O48779.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Signal peptide selection derived cDNAs from Arabidopsis thaliana leaves and guard cells."
    Stracke R., Palme K.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 134-614 (ISOFORM 2).
  4. Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGL33_ARATH
AccessioniPrimary (citable) accession number: O48779
Secondary accession number(s): Q8H7F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: June 1, 1998
Last modified: October 1, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3