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O48779 (BGL33_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucosidase 33
Short name=AtBGLU33
EC=3.2.1.21
Gene names
Name:BGLU33
Ordered Locus Names:At2g32860
ORF Names:T21L14.20
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length614 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Sequence caution

The sequence AAN60253.1 differs from that shown. Reason: Frameshift at position 548.

Isoform 2: The sequence AAN60253.1 differs from that shown. Reason: Frameshift at position 548.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-glucosidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O48779-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Derived from EST data. No experimental confirmation available.
Isoform 2 (identifier: O48779-2)

The sequence of this isoform differs from the canonical sequence as follows:
     323-324: VE → CQE
     435-446: VITNNLSLPDLQ → DSQNNSPHLK

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 614588Beta-glucosidase 33
PRO_0000389595

Regions

Region536 – 5372Substrate binding By similarity

Sites

Active site2631Proton donor By similarity
Active site4791Nucleophile By similarity
Binding site1131Substrate By similarity
Binding site2171Substrate By similarity
Binding site2621Substrate By similarity
Binding site4071Substrate By similarity
Binding site5291Substrate By similarity

Amino acid modifications

Glycosylation3441N-linked (GlcNAc...) Potential
Glycosylation4191N-linked (GlcNAc...) Potential
Glycosylation4321N-linked (GlcNAc...) Potential
Glycosylation4391N-linked (GlcNAc...) Potential
Glycosylation4911N-linked (GlcNAc...) Potential
Disulfide bond282 ↔ 290 By similarity

Natural variations

Alternative sequence323 – 3242VE → CQE in isoform 2.
VSP_038464
Alternative sequence435 – 44612VITNN…LPDLQ → DSQNNSPHLK in isoform 2.
VSP_038465

Experimental info

Sequence conflict2911P → S in AAN60253. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: D1ACCE2438151D29

FASTA61470,190
        10         20         30         40         50         60 
MATATLTLFL GLLALTSTIL SFNADARPQP SDEDLGTIIG PHQTSFDDEI GIVIGPHATV 

        70         80         90        100        110        120 
DDEDIDMDMG TTVGPQTNLN DDDLGTIIGP EFEIHKQDFP ADFIFGTSVS AYQVEGAKKG 

       130        140        150        160        170        180 
SGRGLTSWDE FTHMFPEKVQ QNGDGDEGVD FYTRYKDDIK LMKELNTNGF RFSISWTRIL 

       190        200        210        220        230        240 
PYGTIKKGVN EEGVKFYNDL INELLANGIQ PSVTLFHWES PLALEMEYGG FLNERIVEDF 

       250        260        270        280        290        300 
REFANFCFKE FGDRVKNWAT FNEPSVYSVA GYSKGKKAPG RCSKWQAPKC PTGDSSEEPY 

       310        320        330        340        350        360 
IVAHNQILAH LAAVDEFRNC KKVEGGGKIG IVLVSHWFEP KDPNSSEDVK AARRSLEYQL 

       370        380        390        400        410        420 
GWFLRPLTYG QYPAEMLEDV NIRLREFTPE ESEKLRKSLD FVGLNYYGAF FSTPLAKVNS 

       430        440        450        460        470        480 
SQLNYETDLR VNWTVITNNL SLPDLQTTSM GIVIYPAGLK NILKHIKDEY MDPEIYIMEN 

       490        500        510        520        530        540 
GMDEIDYGTK NITEATNDYG RKEFIKSHIL IMGKSIRMDK VRLKGYYIWS LMDNFEWDKG 

       550        560        570        580        590        600 
YKVRFGLYYV DYNDNMKRYI RSSGKWLSEF LDSKETLHKC YFEGHREKGY APKLFDVEYL 

       610 
EPENSQLSYR SDFM

« Hide

Isoform 2 [UniParc].

Checksum: E43E3BC6BE8579EF
Show »

FASTA61370,135

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Signal peptide selection derived cDNAs from Arabidopsis thaliana leaves and guard cells."
Stracke R., Palme K.
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 134-614 (ISOFORM 2).
[4]"Functional genomic analysis of Arabidopsis thaliana glycoside hydrolase family 1."
Xu Z., Escamilla-Trevino L.L., Zeng L., Lalgondar M., Bevan D.R., Winkel B.S.J., Mohamed A., Cheng C.-L., Shih M.-C., Poulton J.E., Esen A.
Plant Mol. Biol. 55:343-367(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC003033 Genomic DNA. Translation: AAB91979.1.
CP002685 Genomic DNA. Translation: AEC08753.1.
CP002685 Genomic DNA. Translation: AEC08754.1.
AF083694 mRNA. Translation: AAN60253.1. Frameshift.
IPIIPI00528091.
IPI00528849.
PIRT01121.
RefSeqNP_180845.2. NM_128846.3.
NP_973587.1. NM_201858.1.
UniGeneAt.38011.

3D structure databases

HSSPHSSP built from PDB template 1CBG based on UniProtKB P26205.
ProteinModelPortalO48779.
SMRO48779. Positions 94-567.
ModBaseSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbO48779.
PRIDEO48779.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G32860.2; AT2G32860.2; AT2G32860.
GeneID817847.
KEGGath:AT2G32860.

Organism-specific databases

TAIRAt2g32860.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088630.
InParanoidO48779.
KOK01188.
OMAMAEMGFT.
PhylomeDBO48779.
ProtClustDBCLSN2679987.

Gene expression databases

GenevestigatorO48779.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. False negative.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGL33_ARATH
AccessionPrimary (citable) accession number: O48779
Secondary accession number(s): Q8H7F9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: June 1, 1998
Last modified: May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents