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O48779

- BGL33_ARATH

UniProt

O48779 - BGL33_ARATH

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Protein
Beta-glucosidase 33
Gene
BGLU33, At2g32860, T21L14.20
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei113 – 1131Substrate By similarity
Binding sitei217 – 2171Substrate By similarity
Binding sitei262 – 2621Substrate By similarity
Active sitei263 – 2631Proton donor By similarity
Binding sitei407 – 4071Substrate By similarity
Active sitei479 – 4791Nucleophile By similarity
Binding sitei529 – 5291Substrate By similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciARA:AT2G32860-MONOMER.
ARA:GQT-633-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucosidase 33 (EC:3.2.1.21)
Short name:
AtBGLU33
Gene namesi
Name:BGLU33
Ordered Locus Names:At2g32860
ORF Names:T21L14.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G32860.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626 Reviewed prediction
Add
BLAST
Chaini27 – 614588Beta-glucosidase 33
PRO_0000389595Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi282 ↔ 290 By similarity
Glycosylationi344 – 3441N-linked (GlcNAc...) Reviewed prediction
Glycosylationi419 – 4191N-linked (GlcNAc...) Reviewed prediction
Glycosylationi432 – 4321N-linked (GlcNAc...) Reviewed prediction
Glycosylationi439 – 4391N-linked (GlcNAc...) Reviewed prediction
Glycosylationi491 – 4911N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO48779.
PRIDEiO48779.

Expressioni

Gene expression databases

GenevestigatoriO48779.

Structurei

3D structure databases

ProteinModelPortaliO48779.
SMRiO48779. Positions 94-567.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni536 – 5372Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
InParanoidiO48779.
KOiK01188.
OMAiNPQLDYD.
PhylomeDBiO48779.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O48779-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MATATLTLFL GLLALTSTIL SFNADARPQP SDEDLGTIIG PHQTSFDDEI    50
GIVIGPHATV DDEDIDMDMG TTVGPQTNLN DDDLGTIIGP EFEIHKQDFP 100
ADFIFGTSVS AYQVEGAKKG SGRGLTSWDE FTHMFPEKVQ QNGDGDEGVD 150
FYTRYKDDIK LMKELNTNGF RFSISWTRIL PYGTIKKGVN EEGVKFYNDL 200
INELLANGIQ PSVTLFHWES PLALEMEYGG FLNERIVEDF REFANFCFKE 250
FGDRVKNWAT FNEPSVYSVA GYSKGKKAPG RCSKWQAPKC PTGDSSEEPY 300
IVAHNQILAH LAAVDEFRNC KKVEGGGKIG IVLVSHWFEP KDPNSSEDVK 350
AARRSLEYQL GWFLRPLTYG QYPAEMLEDV NIRLREFTPE ESEKLRKSLD 400
FVGLNYYGAF FSTPLAKVNS SQLNYETDLR VNWTVITNNL SLPDLQTTSM 450
GIVIYPAGLK NILKHIKDEY MDPEIYIMEN GMDEIDYGTK NITEATNDYG 500
RKEFIKSHIL IMGKSIRMDK VRLKGYYIWS LMDNFEWDKG YKVRFGLYYV 550
DYNDNMKRYI RSSGKWLSEF LDSKETLHKC YFEGHREKGY APKLFDVEYL 600
EPENSQLSYR SDFM 614

Note: Derived from EST data. No experimental confirmation available.

Length:614
Mass (Da):70,190
Last modified:June 1, 1998 - v1
Checksum:iD1ACCE2438151D29
GO
Isoform 2 (identifier: O48779-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     323-324: VE → CQE
     435-446: VITNNLSLPDLQ → DSQNNSPHLK

Show »
Length:613
Mass (Da):70,135
Checksum:iE43E3BC6BE8579EF
GO

Sequence cautioni

The sequence AAN60253.1 differs from that shown. Reason: Frameshift at position 548.
Isoform 2 : The sequence AAN60253.1 differs from that shown. Reason: Frameshift at position 548.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei323 – 3242VE → CQE in isoform 2.
VSP_038464
Alternative sequencei435 – 44612VITNN…LPDLQ → DSQNNSPHLK in isoform 2.
VSP_038465Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti291 – 2911P → S in AAN60253. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC003033 Genomic DNA. Translation: AAB91979.1.
CP002685 Genomic DNA. Translation: AEC08753.1.
CP002685 Genomic DNA. Translation: AEC08754.1.
AF083694 mRNA. Translation: AAN60253.1. Frameshift.
PIRiT01121.
RefSeqiNP_180845.2. NM_128846.3. [O48779-2]
NP_973587.1. NM_201858.1. [O48779-1]
UniGeneiAt.38011.

Genome annotation databases

EnsemblPlantsiAT2G32860.2; AT2G32860.2; AT2G32860. [O48779-1]
GeneIDi817847.
KEGGiath:AT2G32860.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC003033 Genomic DNA. Translation: AAB91979.1 .
CP002685 Genomic DNA. Translation: AEC08753.1 .
CP002685 Genomic DNA. Translation: AEC08754.1 .
AF083694 mRNA. Translation: AAN60253.1 . Frameshift.
PIRi T01121.
RefSeqi NP_180845.2. NM_128846.3. [O48779-2 ]
NP_973587.1. NM_201858.1. [O48779-1 ]
UniGenei At.38011.

3D structure databases

ProteinModelPortali O48779.
SMRi O48779. Positions 94-567.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbi O48779.
PRIDEi O48779.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G32860.2 ; AT2G32860.2 ; AT2G32860 . [O48779-1 ]
GeneIDi 817847.
KEGGi ath:AT2G32860.

Organism-specific databases

TAIRi AT2G32860.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088630.
InParanoidi O48779.
KOi K01188.
OMAi NPQLDYD.
PhylomeDBi O48779.

Enzyme and pathway databases

BioCyci ARA:AT2G32860-MONOMER.
ARA:GQT-633-MONOMER.

Gene expression databases

Genevestigatori O48779.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Signal peptide selection derived cDNAs from Arabidopsis thaliana leaves and guard cells."
    Stracke R., Palme K.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 134-614 (ISOFORM 2).
  4. Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiBGL33_ARATH
AccessioniPrimary (citable) accession number: O48779
Secondary accession number(s): Q8H7F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: June 1, 1998
Last modified: May 14, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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