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Protein

Protein disulfide-isomerase 2-3

Gene

PDIL2-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds.By similarity

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei60NucleophileBy similarity1
Sitei61Contributes to redox potential valueBy similarity1
Sitei62Contributes to redox potential valueBy similarity1
Active sitei63NucleophileBy similarity1
Sitei122Lowers pKa of C-terminal Cys of first active siteBy similarity1
Active sitei192NucleophileBy similarity1
Sitei193Contributes to redox potential valueBy similarity1
Sitei194Contributes to redox potential valueBy similarity1
Active sitei195NucleophileBy similarity1
Sitei255Lowers pKa of C-terminal Cys of second active siteBy similarity1

GO - Molecular functioni

  • protein disulfide isomerase activity Source: TAIR

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • protein folding Source: GO_Central
  • response to endoplasmic reticulum stress Source: TAIR

Keywordsi

Molecular functionIsomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase 2-3 (EC:5.3.4.1)
Short name:
AtPDIL2-3
Alternative name(s):
Protein disulfide-isomerase 5-2
Short name:
AtPDIL5-2
Protein disulfide-isomerase 9
Short name:
PDI9
Gene namesi
Name:PDIL2-3
Synonyms:PDI9, PDIL5-2
Ordered Locus Names:At2g32920
ORF Names:T21L14.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G32920
TAIRilocus:2059395 AT2G32920

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000040002325 – 440Protein disulfide-isomerase 2-3Add BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi60 ↔ 63Redox-activePROSITE-ProRule annotation
Glycosylationi168N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi192 ↔ 195Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO48773
PRIDEiO48773

PTM databases

SwissPalmiO48773

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Inductioni

By chemically-induced ER stress response.1 Publication

Gene expression databases

ExpressionAtlasiO48773 baseline and differential
GenevisibleiO48773 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G32920.1

Structurei

3D structure databases

ProteinModelPortaliO48773
SMRiO48773
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 136Thioredoxin 1PROSITE-ProRule annotationAdd BLAST112
Domaini154 – 269Thioredoxin 2PROSITE-ProRule annotationAdd BLAST116

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi437 – 440Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0191 Eukaryota
COG0526 LUCA
HOGENOMiHOG000012631
InParanoidiO48773
KOiK09584
OMAiSNELWIV
OrthoDBiEOG09360ATM
PhylomeDBiO48773

Family and domain databases

InterProiView protein in InterPro
IPR005788 Disulphide_isomerase
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF00085 Thioredoxin, 2 hits
SUPFAMiSSF52833 SSF52833, 3 hits
TIGRFAMsiTIGR01126 pdi_dom, 2 hits
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00194 THIOREDOXIN_1, 2 hits
PS51352 THIOREDOXIN_2, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O48773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKSPLTLLT LLTICFGFFD LSSALYGSSS PVVQLTASNF KSKVLNSNGV
60 70 80 90 100
VLVEFFAPWC GHCKALTPTW EKVANILKGV ATVAAIDADA HQSAAQDYGI
110 120 130 140 150
KGFPTIKVFV PGKAPIDYQG ARDAKSIANF AYKQIKGLLS DRLEGKSKPT
160 170 180 190 200
GGGSKEKKSE PSASVELNAS NFDDLVIESN ELWIVEFFAP WCGHCKKLAP
210 220 230 240 250
EWKRAAKNLQ GKVKLGHVNC DVEQSIMSRF KVQGFPTILV FGPDKSSPYP
260 270 280 290 300
YEGARSASAI ESFASELVES SAGPVEVTEL TGPDVMEKKC GSAAICFISF
310 320 330 340 350
LPDILDSKAE GRNKYLEMLL SVAEKFKKQP YSFMWVAAVT QMDLEKRVNV
360 370 380 390 400
GGYGYPAMVA MNVKKGVYAP LKSAFELQHL LEFVKDAGTG GKGNVPMNGT
410 420 430 440
PEIVKTKEWD GKDGELIEED EFSLDELMGG DDAVGSKDEL
Length:440
Mass (Da):47,755
Last modified:June 1, 1998 - v1
Checksum:i2CA62D29A9E9ED01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003033 Genomic DNA Translation: AAB91984.1
CP002685 Genomic DNA Translation: AEC08762.1
AY054270 mRNA Translation: AAL06929.1
AY072321 mRNA Translation: AAL61928.1
AY128728 mRNA Translation: AAM91128.1
PIRiT01115
RefSeqiNP_180851.1, NM_128852.5
UniGeneiAt.12475

Genome annotation databases

EnsemblPlantsiAT2G32920.1; AT2G32920.1; AT2G32920
GeneIDi817854
GrameneiAT2G32920.1; AT2G32920.1; AT2G32920
KEGGiath:AT2G32920

Similar proteinsi

Entry informationi

Entry nameiPDI23_ARATH
AccessioniPrimary (citable) accession number: O48773
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: June 1, 1998
Last modified: April 25, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health