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Protein

Protein disulfide-isomerase 2-3

Gene

PDIL2-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds.By similarity

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei60NucleophileBy similarity1
Sitei61Contributes to redox potential valueBy similarity1
Sitei62Contributes to redox potential valueBy similarity1
Active sitei63NucleophileBy similarity1
Sitei122Lowers pKa of C-terminal Cys of first active siteBy similarity1
Active sitei192NucleophileBy similarity1
Sitei193Contributes to redox potential valueBy similarity1
Sitei194Contributes to redox potential valueBy similarity1
Active sitei195NucleophileBy similarity1
Sitei255Lowers pKa of C-terminal Cys of second active siteBy similarity1

GO - Molecular functioni

  • protein disulfide isomerase activity Source: TAIR

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • protein folding Source: GO_Central
  • response to endoplasmic reticulum stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase 2-3 (EC:5.3.4.1)
Short name:
AtPDIL2-3
Alternative name(s):
Protein disulfide-isomerase 5-2
Short name:
AtPDIL5-2
Protein disulfide-isomerase 9
Short name:
PDI9
Gene namesi
Name:PDIL2-3
Synonyms:PDI9, PDIL5-2
Ordered Locus Names:At2g32920
ORF Names:T21L14.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G32920.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum lumen Source: UniProtKB-SubCell
  • Golgi apparatus Source: TAIR
  • plasma membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000040002325 – 440Protein disulfide-isomerase 2-3Add BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi60 ↔ 63Redox-activePROSITE-ProRule annotation
Glycosylationi168N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi192 ↔ 195Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO48773.
PRIDEiO48773.

PTM databases

SwissPalmiO48773.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Inductioni

By chemically-induced ER stress response.1 Publication

Gene expression databases

GenevisibleiO48773. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G32920.1.

Structurei

3D structure databases

ProteinModelPortaliO48773.
SMRiO48773.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 136Thioredoxin 1PROSITE-ProRule annotationAdd BLAST112
Domaini154 – 269Thioredoxin 2PROSITE-ProRule annotationAdd BLAST116

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi437 – 440Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0191. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000012631.
InParanoidiO48773.
KOiK09584.
OMAiAAICFIS.
OrthoDBiEOG09360ATM.
PhylomeDBiO48773.

Family and domain databases

Gene3Di3.40.30.10. 2 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 3 hits.
TIGRFAMsiTIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O48773-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKSPLTLLT LLTICFGFFD LSSALYGSSS PVVQLTASNF KSKVLNSNGV
60 70 80 90 100
VLVEFFAPWC GHCKALTPTW EKVANILKGV ATVAAIDADA HQSAAQDYGI
110 120 130 140 150
KGFPTIKVFV PGKAPIDYQG ARDAKSIANF AYKQIKGLLS DRLEGKSKPT
160 170 180 190 200
GGGSKEKKSE PSASVELNAS NFDDLVIESN ELWIVEFFAP WCGHCKKLAP
210 220 230 240 250
EWKRAAKNLQ GKVKLGHVNC DVEQSIMSRF KVQGFPTILV FGPDKSSPYP
260 270 280 290 300
YEGARSASAI ESFASELVES SAGPVEVTEL TGPDVMEKKC GSAAICFISF
310 320 330 340 350
LPDILDSKAE GRNKYLEMLL SVAEKFKKQP YSFMWVAAVT QMDLEKRVNV
360 370 380 390 400
GGYGYPAMVA MNVKKGVYAP LKSAFELQHL LEFVKDAGTG GKGNVPMNGT
410 420 430 440
PEIVKTKEWD GKDGELIEED EFSLDELMGG DDAVGSKDEL
Length:440
Mass (Da):47,755
Last modified:June 1, 1998 - v1
Checksum:i2CA62D29A9E9ED01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003033 Genomic DNA. Translation: AAB91984.1.
CP002685 Genomic DNA. Translation: AEC08762.1.
AY054270 mRNA. Translation: AAL06929.1.
AY072321 mRNA. Translation: AAL61928.1.
AY128728 mRNA. Translation: AAM91128.1.
PIRiT01115.
RefSeqiNP_180851.1. NM_128852.5.
UniGeneiAt.12475.

Genome annotation databases

EnsemblPlantsiAT2G32920.1; AT2G32920.1; AT2G32920.
GeneIDi817854.
GrameneiAT2G32920.1; AT2G32920.1; AT2G32920.
KEGGiath:AT2G32920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003033 Genomic DNA. Translation: AAB91984.1.
CP002685 Genomic DNA. Translation: AEC08762.1.
AY054270 mRNA. Translation: AAL06929.1.
AY072321 mRNA. Translation: AAL61928.1.
AY128728 mRNA. Translation: AAM91128.1.
PIRiT01115.
RefSeqiNP_180851.1. NM_128852.5.
UniGeneiAt.12475.

3D structure databases

ProteinModelPortaliO48773.
SMRiO48773.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G32920.1.

PTM databases

SwissPalmiO48773.

Proteomic databases

PaxDbiO48773.
PRIDEiO48773.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G32920.1; AT2G32920.1; AT2G32920.
GeneIDi817854.
GrameneiAT2G32920.1; AT2G32920.1; AT2G32920.
KEGGiath:AT2G32920.

Organism-specific databases

TAIRiAT2G32920.

Phylogenomic databases

eggNOGiKOG0191. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000012631.
InParanoidiO48773.
KOiK09584.
OMAiAAICFIS.
OrthoDBiEOG09360ATM.
PhylomeDBiO48773.

Miscellaneous databases

PROiO48773.

Gene expression databases

GenevisibleiO48773. AT.

Family and domain databases

Gene3Di3.40.30.10. 2 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 3 hits.
TIGRFAMsiTIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDI23_ARATH
AccessioniPrimary (citable) accession number: O48773
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.