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O48773

- PDI23_ARATH

UniProt

O48773 - PDI23_ARATH

Protein

Protein disulfide-isomerase 2-3

Gene

PDIL2-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds.By similarity

    Catalytic activityi

    Catalyzes the rearrangement of -S-S- bonds in proteins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei60 – 601NucleophileBy similarity
    Sitei61 – 611Contributes to redox potential valueBy similarity
    Sitei62 – 621Contributes to redox potential valueBy similarity
    Active sitei63 – 631NucleophileBy similarity
    Sitei122 – 1221Lowers pKa of C-terminal Cys of first active siteBy similarity
    Active sitei192 – 1921NucleophileBy similarity
    Sitei193 – 1931Contributes to redox potential valueBy similarity
    Sitei194 – 1941Contributes to redox potential valueBy similarity
    Active sitei195 – 1951NucleophileBy similarity
    Sitei255 – 2551Lowers pKa of C-terminal Cys of second active siteBy similarity

    GO - Molecular functioni

    1. protein disulfide isomerase activity Source: TAIR

    GO - Biological processi

    1. cell redox homeostasis Source: InterPro
    2. response to endoplasmic reticulum stress Source: TAIR

    Keywords - Molecular functioni

    Isomerase

    Enzyme and pathway databases

    BioCyciARA:AT2G32920-MONOMER.
    ReactomeiREACT_187630. XBP1(S) activates chaperone genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein disulfide-isomerase 2-3 (EC:5.3.4.1)
    Short name:
    AtPDIL2-3
    Alternative name(s):
    Protein disulfide-isomerase 5-2
    Short name:
    AtPDIL5-2
    Protein disulfide-isomerase 9
    Short name:
    PDI9
    Gene namesi
    Name:PDIL2-3
    Synonyms:PDI9, PDIL5-2
    Ordered Locus Names:At2g32920
    ORF Names:T21L14.14
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G32920.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: TAIR
    2. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    3. Golgi apparatus Source: TAIR
    4. plasma membrane Source: TAIR
    5. vacuole Source: TAIR

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 440416Protein disulfide-isomerase 2-3PRO_0000400023Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi60 ↔ 63Redox-activePROSITE-ProRule annotation
    Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi192 ↔ 195Redox-activePROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO48773.
    PRIDEiO48773.

    Expressioni

    Tissue specificityi

    Widely expressed.1 Publication

    Inductioni

    By chemically-induced ER stress response.1 Publication

    Gene expression databases

    GenevestigatoriO48773.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G32920.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO48773.
    SMRiO48773. Positions 24-331.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 136112Thioredoxin 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini154 – 269116Thioredoxin 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi437 – 4404Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the protein disulfide isomerase family.Curated
    Contains 2 thioredoxin domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center, Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG0526.
    HOGENOMiHOG000012631.
    InParanoidiO48773.
    KOiK09584.
    OMAiWKRAAKN.
    PhylomeDBiO48773.

    Family and domain databases

    Gene3Di3.40.30.10. 2 hits.
    InterProiIPR005788. Disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view]
    PfamiPF00085. Thioredoxin. 2 hits.
    [Graphical view]
    SUPFAMiSSF52833. SSF52833. 3 hits.
    TIGRFAMsiTIGR01126. pdi_dom. 2 hits.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 2 hits.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O48773-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYKSPLTLLT LLTICFGFFD LSSALYGSSS PVVQLTASNF KSKVLNSNGV    50
    VLVEFFAPWC GHCKALTPTW EKVANILKGV ATVAAIDADA HQSAAQDYGI 100
    KGFPTIKVFV PGKAPIDYQG ARDAKSIANF AYKQIKGLLS DRLEGKSKPT 150
    GGGSKEKKSE PSASVELNAS NFDDLVIESN ELWIVEFFAP WCGHCKKLAP 200
    EWKRAAKNLQ GKVKLGHVNC DVEQSIMSRF KVQGFPTILV FGPDKSSPYP 250
    YEGARSASAI ESFASELVES SAGPVEVTEL TGPDVMEKKC GSAAICFISF 300
    LPDILDSKAE GRNKYLEMLL SVAEKFKKQP YSFMWVAAVT QMDLEKRVNV 350
    GGYGYPAMVA MNVKKGVYAP LKSAFELQHL LEFVKDAGTG GKGNVPMNGT 400
    PEIVKTKEWD GKDGELIEED EFSLDELMGG DDAVGSKDEL 440
    Length:440
    Mass (Da):47,755
    Last modified:June 1, 1998 - v1
    Checksum:i2CA62D29A9E9ED01
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC003033 Genomic DNA. Translation: AAB91984.1.
    CP002685 Genomic DNA. Translation: AEC08762.1.
    AY054270 mRNA. Translation: AAL06929.1.
    AY072321 mRNA. Translation: AAL61928.1.
    AY128728 mRNA. Translation: AAM91128.1.
    PIRiT01115.
    RefSeqiNP_180851.1. NM_128852.4.
    UniGeneiAt.12475.

    Genome annotation databases

    EnsemblPlantsiAT2G32920.1; AT2G32920.1; AT2G32920.
    GeneIDi817854.
    KEGGiath:AT2G32920.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC003033 Genomic DNA. Translation: AAB91984.1 .
    CP002685 Genomic DNA. Translation: AEC08762.1 .
    AY054270 mRNA. Translation: AAL06929.1 .
    AY072321 mRNA. Translation: AAL61928.1 .
    AY128728 mRNA. Translation: AAM91128.1 .
    PIRi T01115.
    RefSeqi NP_180851.1. NM_128852.4.
    UniGenei At.12475.

    3D structure databases

    ProteinModelPortali O48773.
    SMRi O48773. Positions 24-331.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G32920.1-P.

    Proteomic databases

    PaxDbi O48773.
    PRIDEi O48773.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G32920.1 ; AT2G32920.1 ; AT2G32920 .
    GeneIDi 817854.
    KEGGi ath:AT2G32920.

    Organism-specific databases

    TAIRi AT2G32920.

    Phylogenomic databases

    eggNOGi COG0526.
    HOGENOMi HOG000012631.
    InParanoidi O48773.
    KOi K09584.
    OMAi WKRAAKN.
    PhylomeDBi O48773.

    Enzyme and pathway databases

    BioCyci ARA:AT2G32920-MONOMER.
    Reactomei REACT_187630. XBP1(S) activates chaperone genes.

    Gene expression databases

    Genevestigatori O48773.

    Family and domain databases

    Gene3Di 3.40.30.10. 2 hits.
    InterProi IPR005788. Disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view ]
    Pfami PF00085. Thioredoxin. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52833. SSF52833. 3 hits.
    TIGRFAMsi TIGR01126. pdi_dom. 2 hits.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 2 hits.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins."
      Houston N.L., Fan C., Xiang J.Q., Schulze J.M., Jung R., Boston R.S.
      Plant Physiol. 137:762-778(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    5. "Endoplasmic reticulum stress activates the expression of a sub-group of protein disulfide isomerase genes and AtbZIP60 modulates the response in Arabidopsis thaliana."
      Lu D.-P., Christopher D.A.
      Mol. Genet. Genomics 280:199-210(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION.
    6. "The protein disulfide isomerase gene family in bread wheat (T. aestivum L.)."
      d'Aloisio E., Paolacci A.R., Dhanapal A.P., Tanzarella O.A., Porceddu E., Ciaffi M.
      BMC Plant Biol. 10:101-101(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPDI23_ARATH
    AccessioniPrimary (citable) accession number: O48773
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3