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O48773

- PDI23_ARATH

UniProt

O48773 - PDI23_ARATH

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Protein

Protein disulfide-isomerase 2-3

Gene

PDIL2-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Acts as a protein-folding catalyst that interacts with nascent polypeptides to catalyze the formation, isomerization, and reduction or oxidation of disulfide bonds.By similarity

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei60 – 601NucleophileBy similarity
Sitei61 – 611Contributes to redox potential valueBy similarity
Sitei62 – 621Contributes to redox potential valueBy similarity
Active sitei63 – 631NucleophileBy similarity
Sitei122 – 1221Lowers pKa of C-terminal Cys of first active siteBy similarity
Active sitei192 – 1921NucleophileBy similarity
Sitei193 – 1931Contributes to redox potential valueBy similarity
Sitei194 – 1941Contributes to redox potential valueBy similarity
Active sitei195 – 1951NucleophileBy similarity
Sitei255 – 2551Lowers pKa of C-terminal Cys of second active siteBy similarity

GO - Molecular functioni

  1. protein disulfide isomerase activity Source: TAIR

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
  2. response to endoplasmic reticulum stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciARA:AT2G32920-MONOMER.
ReactomeiREACT_187630. XBP1(S) activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase 2-3 (EC:5.3.4.1)
Short name:
AtPDIL2-3
Alternative name(s):
Protein disulfide-isomerase 5-2
Short name:
AtPDIL5-2
Protein disulfide-isomerase 9
Short name:
PDI9
Gene namesi
Name:PDIL2-3
Synonyms:PDI9, PDIL5-2
Ordered Locus Names:At2g32920
ORF Names:T21L14.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G32920.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: TAIR
  2. Golgi apparatus Source: TAIR
  3. plasma membrane Source: TAIR
  4. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 440416Protein disulfide-isomerase 2-3PRO_0000400023Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi60 ↔ 63Redox-activePROSITE-ProRule annotation
Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi192 ↔ 195Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO48773.
PRIDEiO48773.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Inductioni

By chemically-induced ER stress response.1 Publication

Gene expression databases

GenevestigatoriO48773.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G32920.1-P.

Structurei

3D structure databases

ProteinModelPortaliO48773.
SMRiO48773. Positions 24-331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 136112Thioredoxin 1PROSITE-ProRule annotationAdd
BLAST
Domaini154 – 269116Thioredoxin 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi437 – 4404Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiCOG0526.
HOGENOMiHOG000012631.
InParanoidiO48773.
KOiK09584.
OMAiWKRAAKN.
PhylomeDBiO48773.

Family and domain databases

Gene3Di3.40.30.10. 2 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 3 hits.
TIGRFAMsiTIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O48773-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYKSPLTLLT LLTICFGFFD LSSALYGSSS PVVQLTASNF KSKVLNSNGV
60 70 80 90 100
VLVEFFAPWC GHCKALTPTW EKVANILKGV ATVAAIDADA HQSAAQDYGI
110 120 130 140 150
KGFPTIKVFV PGKAPIDYQG ARDAKSIANF AYKQIKGLLS DRLEGKSKPT
160 170 180 190 200
GGGSKEKKSE PSASVELNAS NFDDLVIESN ELWIVEFFAP WCGHCKKLAP
210 220 230 240 250
EWKRAAKNLQ GKVKLGHVNC DVEQSIMSRF KVQGFPTILV FGPDKSSPYP
260 270 280 290 300
YEGARSASAI ESFASELVES SAGPVEVTEL TGPDVMEKKC GSAAICFISF
310 320 330 340 350
LPDILDSKAE GRNKYLEMLL SVAEKFKKQP YSFMWVAAVT QMDLEKRVNV
360 370 380 390 400
GGYGYPAMVA MNVKKGVYAP LKSAFELQHL LEFVKDAGTG GKGNVPMNGT
410 420 430 440
PEIVKTKEWD GKDGELIEED EFSLDELMGG DDAVGSKDEL
Length:440
Mass (Da):47,755
Last modified:June 1, 1998 - v1
Checksum:i2CA62D29A9E9ED01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003033 Genomic DNA. Translation: AAB91984.1.
CP002685 Genomic DNA. Translation: AEC08762.1.
AY054270 mRNA. Translation: AAL06929.1.
AY072321 mRNA. Translation: AAL61928.1.
AY128728 mRNA. Translation: AAM91128.1.
PIRiT01115.
RefSeqiNP_180851.1. NM_128852.4.
UniGeneiAt.12475.

Genome annotation databases

EnsemblPlantsiAT2G32920.1; AT2G32920.1; AT2G32920.
GeneIDi817854.
KEGGiath:AT2G32920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003033 Genomic DNA. Translation: AAB91984.1 .
CP002685 Genomic DNA. Translation: AEC08762.1 .
AY054270 mRNA. Translation: AAL06929.1 .
AY072321 mRNA. Translation: AAL61928.1 .
AY128728 mRNA. Translation: AAM91128.1 .
PIRi T01115.
RefSeqi NP_180851.1. NM_128852.4.
UniGenei At.12475.

3D structure databases

ProteinModelPortali O48773.
SMRi O48773. Positions 24-331.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G32920.1-P.

Proteomic databases

PaxDbi O48773.
PRIDEi O48773.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G32920.1 ; AT2G32920.1 ; AT2G32920 .
GeneIDi 817854.
KEGGi ath:AT2G32920.

Organism-specific databases

TAIRi AT2G32920.

Phylogenomic databases

eggNOGi COG0526.
HOGENOMi HOG000012631.
InParanoidi O48773.
KOi K09584.
OMAi WKRAAKN.
PhylomeDBi O48773.

Enzyme and pathway databases

BioCyci ARA:AT2G32920-MONOMER.
Reactomei REACT_187630. XBP1(S) activates chaperone genes.

Gene expression databases

Genevestigatori O48773.

Family and domain databases

Gene3Di 3.40.30.10. 2 hits.
InterProi IPR005788. Disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view ]
Pfami PF00085. Thioredoxin. 2 hits.
[Graphical view ]
SUPFAMi SSF52833. SSF52833. 3 hits.
TIGRFAMsi TIGR01126. pdi_dom. 2 hits.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins."
    Houston N.L., Fan C., Xiang J.Q., Schulze J.M., Jung R., Boston R.S.
    Plant Physiol. 137:762-778(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. "Endoplasmic reticulum stress activates the expression of a sub-group of protein disulfide isomerase genes and AtbZIP60 modulates the response in Arabidopsis thaliana."
    Lu D.-P., Christopher D.A.
    Mol. Genet. Genomics 280:199-210(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  6. "The protein disulfide isomerase gene family in bread wheat (T. aestivum L.)."
    d'Aloisio E., Paolacci A.R., Dhanapal A.P., Tanzarella O.A., Porceddu E., Ciaffi M.
    BMC Plant Biol. 10:101-101(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPDI23_ARATH
AccessioniPrimary (citable) accession number: O48773
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3