ID   GUN11_ARATH             Reviewed;         525 AA.
AC   O48766;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   27-MAR-2024, entry version 139.
DE   RecName: Full=Endoglucanase 11;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta glucanase 11;
DE   Flags: Precursor;
GN   OrderedLocusNames=At2g32990; ORFNames=T21L14.7;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=15170254; DOI=10.1007/s00239-003-2571-x;
RA   Libertini E., Li Y., McQueen-Mason S.J.;
RT   "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family.";
RL   J. Mol. Evol. 58:506-515(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000255|PROSITE-ProRule:PRU10140, ECO:0000305}.
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DR   EMBL; AC003033; AAB91971.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC08771.1; -; Genomic_DNA.
DR   EMBL; AY062439; AAL32517.1; -; mRNA.
DR   EMBL; BT008885; AAP68324.1; -; mRNA.
DR   PIR; T01108; T01108.
DR   RefSeq; NP_180858.1; NM_128859.3.
DR   AlphaFoldDB; O48766; -.
DR   SMR; O48766; -.
DR   STRING; 3702.O48766; -.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   PaxDb; 3702-AT2G32990-1; -.
DR   ProteomicsDB; 230177; -.
DR   EnsemblPlants; AT2G32990.1; AT2G32990.1; AT2G32990.
DR   GeneID; 817861; -.
DR   Gramene; AT2G32990.1; AT2G32990.1; AT2G32990.
DR   KEGG; ath:AT2G32990; -.
DR   Araport; AT2G32990; -.
DR   TAIR; AT2G32990; GH9B8.
DR   eggNOG; ENOG502QRF6; Eukaryota.
DR   HOGENOM; CLU_008926_1_4_1; -.
DR   InParanoid; O48766; -.
DR   OMA; CQDNFVD; -.
DR   OrthoDB; 1347382at2759; -.
DR   PhylomeDB; O48766; -.
DR   BioCyc; ARA:AT2G32990-MONOMER; -.
DR   PRO; PR:O48766; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O48766; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   PANTHER; PTHR22298:SF23; ENDOGLUCANASE 11; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS60032; GH9_1; 1.
DR   PROSITE; PS00592; GH9_2; 1.
DR   Genevisible; O48766; AT.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW   Cellulose degradation; Glycoprotein; Glycosidase; Hydrolase;
KW   Polysaccharide degradation; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000255"
FT   CHAIN           32..525
FT                   /note="Endoglucanase 11"
FT                   /id="PRO_0000249263"
FT   ACT_SITE        92
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10140"
FT   ACT_SITE        427
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        479
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   ACT_SITE        488
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10059"
FT   CARBOHYD        337
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   525 AA;  59087 MW;  6E3C3A23165EE44F CRC64;
     MTVMNHRHKQ CHFLLLLLIT VFSAALDGVS SETDVGGFDY GEALSKSLLY FEAQRSGRLP
     YNQRVTWRDH SGLTDGLEQG VDLVGGYHDA GDHVKFGLPM AFTVTMLSWS VIEYGDSLAS
     TGELSHALEA IKWGTDYFIK AHTSPNVLWA EVGDGDTDHY CWQRPEDMTT SRRAFKIDEN
     NPGSDIAGET AAAMAAASIV FRSTNPHYSH LLLHHAQQLF EFGDKYRGKY DESLKVVKSY
     YASVSGYMDE LLWGATWLYR ATDNEHYMSY VVDMAHQLGG LSWAMSEFSW DVKFAGVQLL
     ASMLLKEEKH KQHSKVLQQY KSKADHYLCS ILNKNINGTN VQRTPAGLLY VRQWNNMQYV
     STASFLLTVY SDHLRKSNTD LECHEGTVTP DEMLGFAKSQ IDYILGSNPM ETSYLVGYGP
     KYPIRVHHRG ASIASFKEHK GFIGCTQGYD NWYGRSEPNP SVLVGALVGG PDHQDDFDDR
     RGNYVQTEAC TYNTAPLVGV FARLIELEEQ KLEEEDVSLV ATYKR
//