ID   PORC_ARATH              Reviewed;         401 AA.
AC   O48741;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   16-JUN-2009, entry version 78.
DE   RecName: Full=Protochlorophyllide reductase C, chloroplastic;
DE            Short=PCR C;
DE            EC=1.3.1.33;
DE   AltName: Full=NADPH-protochlorophyllide oxidoreductase C;
DE            Short=POR C;
DE   Flags: Precursor;
GN   Name=PORC; OrderedLocusNames=At1g03630;
GN   ORFNames=F21B7.35, F21B7.24, F21B7_11;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20298658; PubMed=10838072; DOI=10.1016/S0014-5793(00)01568-4;
RA   Oosawa N., Masuda T., Awai K., Fusada N., Shimada H., Ohta H.,
RA   Takamiya K.;
RT   "Identification and light-induced expression of a novel gene of NADPH-
RT   protochlorophyllide oxidoreductase isoform in Arabidopsis thaliana.";
RL   FEBS Lett. 474:133-136(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=21016719; PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA   White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA   Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA   Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA   Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA   Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA   Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA   Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA   Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA   Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA   Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA   Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA   Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA   Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA   Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:816-820(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   CHARACTERIZATION.
RA   Su Q., Armstrong G., Frick G., Apel K.;
RT   "The identification and characterization of a novel NADPH-
RT   protochlorophyllide oxidoreductase C.";
RL   (In) Proceedings of ELSO 2000: European Life Scientist Organization,
RL   pp.72-72, Geneva (2000).
CC   -!- FUNCTION: Phototransformation of protochlorophyllide (Pchlide) to
CC       chlorophyllide (Chlide).
CC   -!- CATALYTIC ACTIVITY: Chlorophyllide a + NADP(+) =
CC       protochlorophyllide + NADPH.
CC   -!- PATHWAY: Porphyrin biosynthesis; chlorophyll biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast. Note=Prolamellar body
CC       of etiolated seedling.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences;
CC       Name=1;
CC         IsoId=O48741-1; Sequence=Displayed;
CC   -!- TISSUE SPECIFICITY: Expressed in flowers, upper leaves, rosette
CC       and cauline leaves, stem. Not detectable in non-photosynthetic
CC       tissues such as roots and seeds.
CC   -!- INDUCTION: By light.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family. POR subfamily.
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DR   EMBL; AB035746; BAA96654.1; -; mRNA.
DR   EMBL; AC002560; AAF86518.1; -; Genomic_DNA.
DR   EMBL; AY048263; AAK82525.1; -; mRNA.
DR   EMBL; AY133569; AAM91399.1; -; mRNA.
DR   EMBL; AY088529; AAM66062.1; -; mRNA.
DR   IPI; IPI00547002; -.
DR   PIR; T00897; T00897.
DR   RefSeq; NP_171860.1; -.
DR   UniGene; At.24740; -.
DR   HSSP; P14061; 1FDU.
DR   PRIDE; O48741; -.
DR   ProMEX; O48741; -.
DR   GeneID; 839009; -.
DR   GenomeReviews; CT485782_GR; AT1G03630.
DR   TAIR; At1g03630; -.
DR   OMA; O48741; RRYHEET.
DR   BioCyc; MetaCyc:AT1G03630-MON; -.
DR   BRENDA; 1.3.1.33; 302.
DR   ArrayExpress; O48741; -.
DR   GO; GO:0009941; C:chloroplast envelope; IDA:TAIR.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IDA:TAIR.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0003959; F:NADPH dehydrogenase activity; IDA:TAIR.
DR   GO; GO:0016630; F:protochlorophyllide reductase activity; IEA:EC.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR005979; Prochl_reduct.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   TIGRFAMs; TIGR01289; LPOR; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Chlorophyll biosynthesis; Chloroplast;
KW   Complete proteome; NADP; Oxidoreductase; Photosynthesis; Plastid;
KW   Transit peptide.
FT   TRANSIT       1     67       Chloroplast (Potential).
FT   CHAIN        68    401       Protochlorophyllide reductase C,
FT                                chloroplastic.
FT                                /FTId=PRO_0000023289.
SQ   SEQUENCE   401 AA;  43883 MW;  6F395276DCE54A3A CRC64;
     MALQAAYSLL PSTISIQKEG KFNASLKETT FTGSSFSNHL RAEKISTLLT IKEQRRQKPR
     FSTGIRAQTV TATPPANEAS PEQKKTERKG TAVITGASSG LGLATAKALA DTGKWHVIMA
     CRNFLKAEKA ARSVGMSKED YTVMHLDLAS LESVKQFVEN FRRTEQPLDV LVCNAAVYQP
     TAKEPSFTAE GFEISVGTNH LGHFLLSRLL LDDLKKSDYP SKRMIIVGSI TGNTNTLAGN
     VPPKANLGDL RGLASGLNGQ NSSMIDGGEF DGAKAYKDSK VCNMLTMQEL HRRYHEETGV
     TFASLYPGCI ATTGLFREHI PLFRLLFPPF QKYITKGYVS EEEAGKRLAQ VVSDPSLGKS
     GVYWSWNNNS SSFENQLSKE ASDAEKAKKL WEVSEKLVGL A
//