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O48741

- PORC_ARATH

UniProt

O48741 - PORC_ARATH

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Protein

Protochlorophyllide reductase C, chloroplastic

Gene

PORC

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).2 Publications

Catalytic activityi

Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.

Pathwayi

GO - Molecular functioni

  1. NADPH dehydrogenase activity Source: TAIR
  2. protochlorophyllide reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB-UniPathway
  2. photosynthesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Photosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT1G03630-MONOMER.
ARA:GQT-1039-MONOMER.
MetaCyc:AT1G03630-MONOMER.
BRENDAi1.3.1.33. 399.
UniPathwayiUPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Protochlorophyllide reductase C, chloroplastic (EC:1.3.1.33)
Short name:
PCR C
Alternative name(s):
NADPH-protochlorophyllide oxidoreductase C
Short name:
POR C
Gene namesi
Name:PORC
Ordered Locus Names:At1g03630
ORF Names:F21B7.24, F21B7.35, F21B7_11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G03630.

Subcellular locationi

Plastidchloroplast membrane 1 Publication
Note: Prolamellar body of etiolated seedling.

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast envelope Source: TAIR
  3. chloroplast thylakoid Source: TAIR
  4. chloroplast thylakoid membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid

Pathology & Biotechi

Disruption phenotypei

No visible phenotype at the levels of the whole plant or chloroplast ultrastructure; due to the redundancy with PORB. Porb and porc double mutants have a seedling-lethal pale-yellow xantha phenotype at the cotyledon stage, contain only small amounts of Chla, and possess chloroplasts with mostly unstacked thylakoid membranes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6767ChloroplastSequence AnalysisAdd
BLAST
Chaini68 – 401334Protochlorophyllide reductase C, chloroplasticPRO_0000023289Add
BLAST

Proteomic databases

PaxDbiO48741.
PRIDEiO48741.

Expressioni

Tissue specificityi

Expressed in flowers, upper leaves, rosette and cauline leaves, stem. Not detectable in non-photosynthetic tissues such as roots and seeds.1 Publication

Inductioni

Up-regulated by light. Not under circadian regulation.2 Publications

Gene expression databases

ExpressionAtlasiO48741. baseline and differential.
GenevestigatoriO48741.

Interactioni

Subunit structurei

Part of the FLU-containing chloroplast membrane complex composed of FLU, CRD1, PORB, PORC, CHLP and HEMA1.1 Publication

Protein-protein interaction databases

BioGridi24247. 9 interactions.
IntActiO48741. 13 interactions.
MINTiMINT-8058859.

Structurei

3D structure databases

ProteinModelPortaliO48741.
SMRiO48741. Positions 91-293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1028.
InParanoidiO48741.
KOiK00218.
OMAiKGSYTIV.
PhylomeDBiO48741.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
TIGRFAMsiTIGR01289. LPOR. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O48741-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALQAAYSLL PSTISIQKEG KFNASLKETT FTGSSFSNHL RAEKISTLLT
60 70 80 90 100
IKEQRRQKPR FSTGIRAQTV TATPPANEAS PEQKKTERKG TAVITGASSG
110 120 130 140 150
LGLATAKALA DTGKWHVIMA CRNFLKAEKA ARSVGMSKED YTVMHLDLAS
160 170 180 190 200
LESVKQFVEN FRRTEQPLDV LVCNAAVYQP TAKEPSFTAE GFEISVGTNH
210 220 230 240 250
LGHFLLSRLL LDDLKKSDYP SKRMIIVGSI TGNTNTLAGN VPPKANLGDL
260 270 280 290 300
RGLASGLNGQ NSSMIDGGEF DGAKAYKDSK VCNMLTMQEL HRRYHEETGV
310 320 330 340 350
TFASLYPGCI ATTGLFREHI PLFRLLFPPF QKYITKGYVS EEEAGKRLAQ
360 370 380 390 400
VVSDPSLGKS GVYWSWNNNS SSFENQLSKE ASDAEKAKKL WEVSEKLVGL

A
Length:401
Mass (Da):43,883
Last modified:June 1, 1998 - v1
Checksum:i6F395276DCE54A3A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035746 mRNA. Translation: BAA96654.1.
AC002560 Genomic DNA. Translation: AAF86518.1.
CP002684 Genomic DNA. Translation: AEE27591.1.
AY048263 mRNA. Translation: AAK82525.1.
AY133569 mRNA. Translation: AAM91399.1.
AY088529 mRNA. Translation: AAM66062.1.
PIRiT00897.
RefSeqiNP_171860.1. NM_100243.3. [O48741-1]
UniGeneiAt.24740.

Genome annotation databases

EnsemblPlantsiAT1G03630.1; AT1G03630.1; AT1G03630. [O48741-1]
GeneIDi839009.
KEGGiath:AT1G03630.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035746 mRNA. Translation: BAA96654.1 .
AC002560 Genomic DNA. Translation: AAF86518.1 .
CP002684 Genomic DNA. Translation: AEE27591.1 .
AY048263 mRNA. Translation: AAK82525.1 .
AY133569 mRNA. Translation: AAM91399.1 .
AY088529 mRNA. Translation: AAM66062.1 .
PIRi T00897.
RefSeqi NP_171860.1. NM_100243.3. [O48741-1 ]
UniGenei At.24740.

3D structure databases

ProteinModelPortali O48741.
SMRi O48741. Positions 91-293.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 24247. 9 interactions.
IntActi O48741. 13 interactions.
MINTi MINT-8058859.

Proteomic databases

PaxDbi O48741.
PRIDEi O48741.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G03630.1 ; AT1G03630.1 ; AT1G03630 . [O48741-1 ]
GeneIDi 839009.
KEGGi ath:AT1G03630.

Organism-specific databases

TAIRi AT1G03630.

Phylogenomic databases

eggNOGi COG1028.
InParanoidi O48741.
KOi K00218.
OMAi KGSYTIV.
PhylomeDBi O48741.

Enzyme and pathway databases

UniPathwayi UPA00668 .
BioCyci ARA:AT1G03630-MONOMER.
ARA:GQT-1039-MONOMER.
MetaCyc:AT1G03630-MONOMER.
BRENDAi 1.3.1.33. 399.

Gene expression databases

ExpressionAtlasi O48741. baseline and differential.
Genevestigatori O48741.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
TIGRFAMsi TIGR01289. LPOR. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and light-induced expression of a novel gene of NADPH-protochlorophyllide oxidoreductase isoform in Arabidopsis thaliana."
    Oosawa N., Masuda T., Awai K., Fusada N., Shimada H., Ohta H., Takamiya K.
    FEBS Lett. 474:133-136(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY LIGHT, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "The identification and characterization of a novel NADPH-protochlorophyllide oxidoreductase C."
    Su Q., Armstrong G., Frick G., Apel K.
    (In) Proceedings of ELSO 2000: European Life Scientist Organization, pp.72-72, Geneva (2000)
    Cited for: CHARACTERIZATION.
  7. "POR C of Arabidopsis thaliana: a third light- and NADPH-dependent protochlorophyllide oxidoreductase that is differentially regulated by light."
    Su Q., Frick G., Armstrong G., Apel K.
    Plant Mol. Biol. 47:805-813(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY LIGHT.
    Strain: cv. Columbia.
  8. "An Arabidopsis porB porC double mutant lacking light-dependent NADPH:protochlorophyllide oxidoreductases B and C is highly chlorophyll-deficient and developmentally arrested."
    Frick G., Su Q., Apel K., Armstrong G.A.
    Plant J. 35:141-153(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  9. "FLU, a negative feedback regulator of tetrapyrrole biosynthesis, is physically linked to the final steps of the Mg(++)-branch of this pathway."
    Kauss D., Bischof S., Steiner S., Apel K., Meskauskiene R.
    FEBS Lett. 586:211-216(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE FLU-CONTAINING CHLOROPLAST MEMBRANE COMPLEX.

Entry informationi

Entry nameiPORC_ARATH
AccessioniPrimary (citable) accession number: O48741
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3