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O48741 (PORC_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protochlorophyllide reductase C, chloroplastic

Short name=PCR C
EC=1.3.1.33
Alternative name(s):
NADPH-protochlorophyllide oxidoreductase C
Short name=POR C
Gene names
Name:PORC
Ordered Locus Names:At1g03630
ORF Names:F21B7.24, F21B7.35, F21B7_11
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide). Ref.7 Ref.8

Catalytic activity

Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.

Pathway

Porphyrin-containing compound metabolism; chlorophyll biosynthesis.

Subunit structure

Part of the FLU-containing chloroplast membrane complex composed of FLU, CRD1, PORB, PORC, CHLP and HEMA1. Ref.9

Subcellular location

Plastidchloroplast membrane. Note: Prolamellar body of etiolated seedling. Ref.9

Tissue specificity

Expressed in flowers, upper leaves, rosette and cauline leaves, stem. Not detectable in non-photosynthetic tissues such as roots and seeds. Ref.1

Induction

Up-regulated by light. Not under circadian regulation. Ref.1 Ref.7

Disruption phenotype

No visible phenotype at the levels of the whole plant or chloroplast ultrastructure; due to the redundancy with PORB. Porb and porc double mutants have a seedling-lethal pale-yellow xantha phenotype at the cotyledon stage, contain only small amounts of Chla, and possess chloroplasts with mostly unstacked thylakoid membranes. Ref.8

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family. POR subfamily.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: O48741-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6767Chloroplast Potential
Chain68 – 401334Protochlorophyllide reductase C, chloroplastic
PRO_0000023289

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 6F395276DCE54A3A

FASTA40143,883
        10         20         30         40         50         60 
MALQAAYSLL PSTISIQKEG KFNASLKETT FTGSSFSNHL RAEKISTLLT IKEQRRQKPR 

        70         80         90        100        110        120 
FSTGIRAQTV TATPPANEAS PEQKKTERKG TAVITGASSG LGLATAKALA DTGKWHVIMA 

       130        140        150        160        170        180 
CRNFLKAEKA ARSVGMSKED YTVMHLDLAS LESVKQFVEN FRRTEQPLDV LVCNAAVYQP 

       190        200        210        220        230        240 
TAKEPSFTAE GFEISVGTNH LGHFLLSRLL LDDLKKSDYP SKRMIIVGSI TGNTNTLAGN 

       250        260        270        280        290        300 
VPPKANLGDL RGLASGLNGQ NSSMIDGGEF DGAKAYKDSK VCNMLTMQEL HRRYHEETGV 

       310        320        330        340        350        360 
TFASLYPGCI ATTGLFREHI PLFRLLFPPF QKYITKGYVS EEEAGKRLAQ VVSDPSLGKS 

       370        380        390        400 
GVYWSWNNNS SSFENQLSKE ASDAEKAKKL WEVSEKLVGL A 

« Hide

References

« Hide 'large scale' references
[1]"Identification and light-induced expression of a novel gene of NADPH-protochlorophyllide oxidoreductase isoform in Arabidopsis thaliana."
Oosawa N., Masuda T., Awai K., Fusada N., Shimada H., Ohta H., Takamiya K.
FEBS Lett. 474:133-136(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY LIGHT, TISSUE SPECIFICITY.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"The identification and characterization of a novel NADPH-protochlorophyllide oxidoreductase C."
Su Q., Armstrong G., Frick G., Apel K.
(In) Proceedings of ELSO 2000: European Life Scientist Organization, pp.72-72, Geneva (2000)
Cited for: CHARACTERIZATION.
[7]"POR C of Arabidopsis thaliana: a third light- and NADPH-dependent protochlorophyllide oxidoreductase that is differentially regulated by light."
Su Q., Frick G., Armstrong G., Apel K.
Plant Mol. Biol. 47:805-813(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION BY LIGHT.
Strain: cv. Columbia.
[8]"An Arabidopsis porB porC double mutant lacking light-dependent NADPH:protochlorophyllide oxidoreductases B and C is highly chlorophyll-deficient and developmentally arrested."
Frick G., Su Q., Apel K., Armstrong G.A.
Plant J. 35:141-153(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[9]"FLU, a negative feedback regulator of tetrapyrrole biosynthesis, is physically linked to the final steps of the Mg(++)-branch of this pathway."
Kauss D., Bischof S., Steiner S., Apel K., Meskauskiene R.
FEBS Lett. 586:211-216(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE FLU-CONTAINING CHLOROPLAST MEMBRANE COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB035746 mRNA. Translation: BAA96654.1.
AC002560 Genomic DNA. Translation: AAF86518.1.
CP002684 Genomic DNA. Translation: AEE27591.1.
AY048263 mRNA. Translation: AAK82525.1.
AY133569 mRNA. Translation: AAM91399.1.
AY088529 mRNA. Translation: AAM66062.1.
PIRT00897.
RefSeqNP_171860.1. NM_100243.3. [O48741-1]
UniGeneAt.24740.

3D structure databases

ProteinModelPortalO48741.
SMRO48741. Positions 87-365.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid24247. 9 interactions.
IntActO48741. 13 interactions.
MINTMINT-8058859.

Proteomic databases

PaxDbO48741.
PRIDEO48741.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G03630.1; AT1G03630.1; AT1G03630. [O48741-1]
GeneID839009.
KEGGath:AT1G03630.

Organism-specific databases

TAIRAT1G03630.

Phylogenomic databases

eggNOGCOG1028.
InParanoidO48741.
KOK00218.
OMAKGSYTIV.
PhylomeDBO48741.

Enzyme and pathway databases

BioCycARA:AT1G03630-MONOMER.
ARA:GQT-1039-MONOMER.
MetaCyc:AT1G03630-MONOMER.
BRENDA1.3.1.33. 399.
UniPathwayUPA00668.

Gene expression databases

ArrayExpressO48741.
GenevestigatorO48741.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
TIGRFAMsTIGR01289. LPOR. 1 hit.
ProtoNetSearch...

Entry information

Entry namePORC_ARATH
AccessionPrimary (citable) accession number: O48741
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: June 11, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names