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O48741

- PORC_ARATH

UniProt

O48741 - PORC_ARATH

Protein

Protochlorophyllide reductase C, chloroplastic

Gene

PORC

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).2 Publications

    Catalytic activityi

    Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.

    Pathwayi

    GO - Molecular functioni

    1. NADPH dehydrogenase activity Source: TAIR
    2. protochlorophyllide reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. chlorophyll biosynthetic process Source: UniProtKB-UniPathway
    2. photosynthesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Chlorophyll biosynthesis, Photosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciARA:AT1G03630-MONOMER.
    ARA:GQT-1039-MONOMER.
    MetaCyc:AT1G03630-MONOMER.
    BRENDAi1.3.1.33. 399.
    UniPathwayiUPA00668.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protochlorophyllide reductase C, chloroplastic (EC:1.3.1.33)
    Short name:
    PCR C
    Alternative name(s):
    NADPH-protochlorophyllide oxidoreductase C
    Short name:
    POR C
    Gene namesi
    Name:PORC
    Ordered Locus Names:At1g03630
    ORF Names:F21B7.24, F21B7.35, F21B7_11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G03630.

    Subcellular locationi

    Plastidchloroplast membrane 1 Publication
    Note: Prolamellar body of etiolated seedling.

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast envelope Source: TAIR
    3. chloroplast membrane Source: UniProtKB-SubCell
    4. chloroplast thylakoid Source: TAIR
    5. chloroplast thylakoid membrane Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype at the levels of the whole plant or chloroplast ultrastructure; due to the redundancy with PORB. Porb and porc double mutants have a seedling-lethal pale-yellow xantha phenotype at the cotyledon stage, contain only small amounts of Chla, and possess chloroplasts with mostly unstacked thylakoid membranes.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 6767ChloroplastSequence AnalysisAdd
    BLAST
    Chaini68 – 401334Protochlorophyllide reductase C, chloroplasticPRO_0000023289Add
    BLAST

    Proteomic databases

    PaxDbiO48741.
    PRIDEiO48741.

    Expressioni

    Tissue specificityi

    Expressed in flowers, upper leaves, rosette and cauline leaves, stem. Not detectable in non-photosynthetic tissues such as roots and seeds.1 Publication

    Inductioni

    Up-regulated by light. Not under circadian regulation.2 Publications

    Gene expression databases

    ArrayExpressiO48741.
    GenevestigatoriO48741.

    Interactioni

    Subunit structurei

    Part of the FLU-containing chloroplast membrane complex composed of FLU, CRD1, PORB, PORC, CHLP and HEMA1.1 Publication

    Protein-protein interaction databases

    BioGridi24247. 9 interactions.
    IntActiO48741. 13 interactions.
    MINTiMINT-8058859.

    Structurei

    3D structure databases

    ProteinModelPortaliO48741.
    SMRiO48741. Positions 87-365.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG1028.
    InParanoidiO48741.
    KOiK00218.
    OMAiKGSYTIV.
    PhylomeDBiO48741.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR005979. Prochl_reduct.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    TIGRFAMsiTIGR01289. LPOR. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: O48741-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MALQAAYSLL PSTISIQKEG KFNASLKETT FTGSSFSNHL RAEKISTLLT    50
    IKEQRRQKPR FSTGIRAQTV TATPPANEAS PEQKKTERKG TAVITGASSG 100
    LGLATAKALA DTGKWHVIMA CRNFLKAEKA ARSVGMSKED YTVMHLDLAS 150
    LESVKQFVEN FRRTEQPLDV LVCNAAVYQP TAKEPSFTAE GFEISVGTNH 200
    LGHFLLSRLL LDDLKKSDYP SKRMIIVGSI TGNTNTLAGN VPPKANLGDL 250
    RGLASGLNGQ NSSMIDGGEF DGAKAYKDSK VCNMLTMQEL HRRYHEETGV 300
    TFASLYPGCI ATTGLFREHI PLFRLLFPPF QKYITKGYVS EEEAGKRLAQ 350
    VVSDPSLGKS GVYWSWNNNS SSFENQLSKE ASDAEKAKKL WEVSEKLVGL 400
    A 401
    Length:401
    Mass (Da):43,883
    Last modified:June 1, 1998 - v1
    Checksum:i6F395276DCE54A3A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB035746 mRNA. Translation: BAA96654.1.
    AC002560 Genomic DNA. Translation: AAF86518.1.
    CP002684 Genomic DNA. Translation: AEE27591.1.
    AY048263 mRNA. Translation: AAK82525.1.
    AY133569 mRNA. Translation: AAM91399.1.
    AY088529 mRNA. Translation: AAM66062.1.
    PIRiT00897.
    RefSeqiNP_171860.1. NM_100243.3. [O48741-1]
    UniGeneiAt.24740.

    Genome annotation databases

    EnsemblPlantsiAT1G03630.1; AT1G03630.1; AT1G03630. [O48741-1]
    GeneIDi839009.
    KEGGiath:AT1G03630.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB035746 mRNA. Translation: BAA96654.1 .
    AC002560 Genomic DNA. Translation: AAF86518.1 .
    CP002684 Genomic DNA. Translation: AEE27591.1 .
    AY048263 mRNA. Translation: AAK82525.1 .
    AY133569 mRNA. Translation: AAM91399.1 .
    AY088529 mRNA. Translation: AAM66062.1 .
    PIRi T00897.
    RefSeqi NP_171860.1. NM_100243.3. [O48741-1 ]
    UniGenei At.24740.

    3D structure databases

    ProteinModelPortali O48741.
    SMRi O48741. Positions 87-365.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 24247. 9 interactions.
    IntActi O48741. 13 interactions.
    MINTi MINT-8058859.

    Proteomic databases

    PaxDbi O48741.
    PRIDEi O48741.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G03630.1 ; AT1G03630.1 ; AT1G03630 . [O48741-1 ]
    GeneIDi 839009.
    KEGGi ath:AT1G03630.

    Organism-specific databases

    TAIRi AT1G03630.

    Phylogenomic databases

    eggNOGi COG1028.
    InParanoidi O48741.
    KOi K00218.
    OMAi KGSYTIV.
    PhylomeDBi O48741.

    Enzyme and pathway databases

    UniPathwayi UPA00668 .
    BioCyci ARA:AT1G03630-MONOMER.
    ARA:GQT-1039-MONOMER.
    MetaCyc:AT1G03630-MONOMER.
    BRENDAi 1.3.1.33. 399.

    Gene expression databases

    ArrayExpressi O48741.
    Genevestigatori O48741.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR005979. Prochl_reduct.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    TIGRFAMsi TIGR01289. LPOR. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification and light-induced expression of a novel gene of NADPH-protochlorophyllide oxidoreductase isoform in Arabidopsis thaliana."
      Oosawa N., Masuda T., Awai K., Fusada N., Shimada H., Ohta H., Takamiya K.
      FEBS Lett. 474:133-136(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY LIGHT, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "The identification and characterization of a novel NADPH-protochlorophyllide oxidoreductase C."
      Su Q., Armstrong G., Frick G., Apel K.
      (In) Proceedings of ELSO 2000: European Life Scientist Organization, pp.72-72, Geneva (2000)
      Cited for: CHARACTERIZATION.
    7. "POR C of Arabidopsis thaliana: a third light- and NADPH-dependent protochlorophyllide oxidoreductase that is differentially regulated by light."
      Su Q., Frick G., Armstrong G., Apel K.
      Plant Mol. Biol. 47:805-813(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION BY LIGHT.
      Strain: cv. Columbia.
    8. "An Arabidopsis porB porC double mutant lacking light-dependent NADPH:protochlorophyllide oxidoreductases B and C is highly chlorophyll-deficient and developmentally arrested."
      Frick G., Su Q., Apel K., Armstrong G.A.
      Plant J. 35:141-153(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    9. "FLU, a negative feedback regulator of tetrapyrrole biosynthesis, is physically linked to the final steps of the Mg(++)-branch of this pathway."
      Kauss D., Bischof S., Steiner S., Apel K., Meskauskiene R.
      FEBS Lett. 586:211-216(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE FLU-CONTAINING CHLOROPLAST MEMBRANE COMPLEX.

    Entry informationi

    Entry nameiPORC_ARATH
    AccessioniPrimary (citable) accession number: O48741
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3