O48741 (PORC_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protochlorophyllide reductase C, chloroplastic
Short name=PCR C
EC=1.3.1.33

Alternative name(s):
NADPH-protochlorophyllide oxidoreductase C
Short name=POR C

Gene names

Name:	PORC
Ordered Locus Names:	At1g03630
ORF Names:	F21B7.24, F21B7.35, F21B7_11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).
Catalytic activity	Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis.
Subcellular location	Plastid › chloroplast. Note: Prolamellar body of etiolated seedling.
Tissue specificity	Expressed in flowers, upper leaves, rosette and cauline leaves, stem. Not detectable in non-photosynthetic tissues such as roots and seeds.
Induction	By light.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. POR subfamily.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast envelope Inferred from direct assay PubMed 12938931PubMed 20061580. Source: TAIR chloroplast thylakoid membrane Inferred from direct assay PubMed 15322131. Source: TAIR
Molecular_function	NADPH dehydrogenase activity Inferred from direct assay PubMed 11785941. Source: TAIR nucleotide binding Inferred from electronic annotation. Source: InterPro protochlorophyllide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O48741-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 67	67	Chloroplast Potential
	Chain	68 – 401	334	Protochlorophyllide reductase C, chloroplastic		PRO_0000023289

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 6F395276DCE54A3A	FASTA	401	43,883
	10 20 30 40 50 60 MALQAAYSLL PSTISIQKEG KFNASLKETT FTGSSFSNHL RAEKISTLLT IKEQRRQKPR 70 80 90 100 110 120 FSTGIRAQTV TATPPANEAS PEQKKTERKG TAVITGASSG LGLATAKALA DTGKWHVIMA 130 140 150 160 170 180 CRNFLKAEKA ARSVGMSKED YTVMHLDLAS LESVKQFVEN FRRTEQPLDV LVCNAAVYQP 190 200 210 220 230 240 TAKEPSFTAE GFEISVGTNH LGHFLLSRLL LDDLKKSDYP SKRMIIVGSI TGNTNTLAGN 250 260 270 280 290 300 VPPKANLGDL RGLASGLNGQ NSSMIDGGEF DGAKAYKDSK VCNMLTMQEL HRRYHEETGV 310 320 330 340 350 360 TFASLYPGCI ATTGLFREHI PLFRLLFPPF QKYITKGYVS EEEAGKRLAQ VVSDPSLGKS 370 380 390 400 GVYWSWNNNS SSFENQLSKE ASDAEKAKKL WEVSEKLVGL A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and light-induced expression of a novel gene of NADPH-protochlorophyllide oxidoreductase isoform in Arabidopsis thaliana." Oosawa N., Masuda T., Awai K., Fusada N., Shimada H., Ohta H., Takamiya K. FEBS Lett. 474:133-136(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The identification and characterization of a novel NADPH-protochlorophyllide oxidoreductase C." Su Q., Armstrong G., Frick G., Apel K. (In) Proceedings of ELSO 2000: European Life Scientist Organization, pp.72-72, Geneva (2000) Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB035746 mRNA. Translation: BAA96654.1. AC002560 Genomic DNA. Translation: AAF86518.1. CP002684 Genomic DNA. Translation: AEE27591.1. AY048263 mRNA. Translation: AAK82525.1. AY133569 mRNA. Translation: AAM91399.1. AY088529 mRNA. Translation: AAM66062.1.
IPI	IPI00547002.
PIR	T00897.
RefSeq	NP_171860.1. NM_100243.3.
UniGene	At.24740.
3D structure databases
ProteinModelPortal	O48741.
SMR	O48741. Positions 91-293.
ModBase	Search...
Protein-protein interaction databases
IntAct	O48741. 11 interactions.
Proteomic databases
PaxDb	O48741.
PRIDE	O48741.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G03630.1; AT1G03630.1; AT1G03630.
GeneID	839009.
KEGG	ath:AT1G03630.
Organism-specific databases
TAIR	At1g03630.
Phylogenomic databases
eggNOG	COG1028.
InParanoid	O48741.
KO	K00218.
OMA	WSWNNNS.
PhylomeDB	O48741.
ProtClustDB	PLN00015.
Enzyme and pathway databases
BioCyc	ARA:AT1G03630-MONOMER. MetaCyc:AT1G03630-MONOMER.
BRENDA	1.3.1.33. 399.
UniPathway	UPA00668.
Gene expression databases
ArrayExpress	O48741.
Genevestigator	O48741.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR005979. Prochl_reduct. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
TIGRFAMs	TIGR01289. LPOR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PORC_ARATH

Accession

Primary (citable) accession number: O48741

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program