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O48721 (HEM4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uroporphyrinogen-III synthase, chloroplastic

Short name=AtUROS
EC=4.2.1.75
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene names
Name:UROS
Synonyms:HEMD
Ordered Locus Names:At2g26540
ORF Names:T9J22.21
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length321 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, a precursor of tetrapyrroles such as chlorophyll, heme and phycobilins. Ref.1

Catalytic activity

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.

Porphyrin-containing compound metabolism; chlorophyll biosynthesis.

Subcellular location

Plastidchloroplast Ref.1.

Sequence similarities

Belongs to the uroporphyrinogen-III synthase family.

Ontologies

Keywords
   Biological processChlorophyll biosynthesis
Porphyrin biosynthesis
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processchlorophyll biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

protoporphyrinogen IX biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

uroporphyrinogen III biosynthetic process

Inferred from direct assay Ref.1. Source: UniProtKB

   Cellular_componentchloroplast

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionuroporphyrinogen-III synthase activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 8181Chloroplast Potential
Chain82 – 321240Uroporphyrinogen-III synthase, chloroplastic
PRO_0000376067

Experimental info

Sequence conflict291I → V in CAC85287. Ref.1
Sequence conflict2181V → I in AAM64811. Ref.5

Sequences

Sequence LengthMass (Da)Tools
O48721 [UniParc].

Last modified June 1, 2002. Version 2.
Checksum: 747E4E3C2DCEBD08

FASTA32134,225
        10         20         30         40         50         60 
MALLLLSHCS ILSFQPPLSS SSSFHSSHIQ SLSKPVFASP SPIRNSISSS VSSSSSSVSS 

        70         80         90        100        110        120 
SNSIPQVVVT RERGKNNQII KALEKNGISS LELPLIQHAR GPDFDRLASV LNDKSFDWII 

       130        140        150        160        170        180 
ITSPEAGSVF LEAWKTASSP EVQIGVVGAG TARVFEEAMK SADGLLHVAF TPSKATGKVL 

       190        200        210        220        230        240 
ASELPEKVGK RSSVLYPASL KAGNDIVEGL SKRGFEVVRL NTYTTVPVQS VDTVLLQQAL 

       250        260        270        280        290        300 
SAPVLSVASP SAVRAWLHLI QNEEQWSNYV ACIGETTASA ARRLGLKNVY YPEKPGLEGW 

       310        320 
VESIMEALGA HADSSNPSSR N 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of the Arabidopsis gene encoding the tetrapyrrole biosynthesis enzyme uroporphyrinogen III synthase."
Tan F.-C., Cheng Q., Saha K., Heinemann I.U., Jahn M., Jahn D., Smith A.G.
Biochem. J. 410:291-299(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 132-321.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ314569 mRNA. Translation: CAC85287.1.
AC002505 Genomic DNA. Translation: AAC14502.2.
CP002685 Genomic DNA. Translation: AEC07854.1.
BT026116 mRNA. Translation: ABG48472.1.
AY087255 mRNA. Translation: AAM64811.1.
AY065389 mRNA. Translation: AAL38830.2.
PIRT00987.
RefSeqNP_565625.1. NM_128211.3.
UniGeneAt.28505.

3D structure databases

ProteinModelPortalO48721.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid2547. 1 interaction.

Proteomic databases

PaxDbO48721.
PRIDEO48721.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G26540.1; AT2G26540.1; AT2G26540.
GeneID817195.
KEGGath:AT2G26540.

Organism-specific databases

TAIRAT2G26540.

Phylogenomic databases

eggNOGCOG1587.
HOGENOMHOG000012769.
InParanoidO48721.
KOK01719.
OMAWIIITSP.
PhylomeDBO48721.

Enzyme and pathway databases

BioCycARA:AT2G26540-MONOMER.
MetaCyc:AT2G26540-MONOMER.
UniPathwayUPA00251; UER00320.
UPA00668.

Gene expression databases

GenevestigatorO48721.

Family and domain databases

InterProIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMSSF69618. SSF69618. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHEM4_ARATH
AccessionPrimary (citable) accession number: O48721
Secondary accession number(s): Q8LBE8, Q8RVZ2, Q8VZ12
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names