O48721 (HEM4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uroporphyrinogen-III synthase, chloroplastic
Short name=AtUROS
EC=4.2.1.75

Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase

Gene names

Name:	UROS
Synonyms:	HEMD
Ordered Locus Names:	At2g26540
ORF Names:	T9J22.21

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, a precursor of tetrapyrroles such as chlorophyll, heme and phycobilins. Ref.1
Catalytic activity	Hydroxymethylbilane = uroporphyrinogen III + H2O.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.
Subcellular location	Plastid › chloroplast Ref.1.
Sequence similarities	Belongs to the uroporphyrinogen-III synthase family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway uroporphyrinogen III biosynthetic process Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	chloroplast Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	uroporphyrinogen-III synthase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 81	81	Chloroplast Potential
	Chain	82 – 321	240	Uroporphyrinogen-III synthase, chloroplastic		PRO_0000376067
Experimental info
	Sequence conflict	29	1	I → V in CAC85287. Ref.1
	Sequence conflict	218	1	V → I in AAM64811. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	O48721 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: 747E4E3C2DCEBD08	FASTA	321	34,225
	10 20 30 40 50 60 MALLLLSHCS ILSFQPPLSS SSSFHSSHIQ SLSKPVFASP SPIRNSISSS VSSSSSSVSS 70 80 90 100 110 120 SNSIPQVVVT RERGKNNQII KALEKNGISS LELPLIQHAR GPDFDRLASV LNDKSFDWII 130 140 150 160 170 180 ITSPEAGSVF LEAWKTASSP EVQIGVVGAG TARVFEEAMK SADGLLHVAF TPSKATGKVL 190 200 210 220 230 240 ASELPEKVGK RSSVLYPASL KAGNDIVEGL SKRGFEVVRL NTYTTVPVQS VDTVLLQQAL 250 260 270 280 290 300 SAPVLSVASP SAVRAWLHLI QNEEQWSNYV ACIGETTASA ARRLGLKNVY YPEKPGLEGW 310 320 VESIMEALGA HADSSNPSSR N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of the Arabidopsis gene encoding the tetrapyrrole biosynthesis enzyme uroporphyrinogen III synthase." Tan F.-C., Cheng Q., Saha K., Heinemann I.U., Jahn M., Jahn D., Smith A.G. Biochem. J. 410:291-299(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 132-321. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ314569 mRNA. Translation: CAC85287.1. AC002505 Genomic DNA. Translation: AAC14502.2. CP002685 Genomic DNA. Translation: AEC07854.1. BT026116 mRNA. Translation: ABG48472.1. AY087255 mRNA. Translation: AAM64811.1. AY065389 mRNA. Translation: AAL38830.2.
IPI	IPI00537335.
PIR	T00987.
RefSeq	NP_565625.1. NM_128211.3.
UniGene	At.28505.
3D structure databases
ProteinModelPortal	O48721.
ModBase	Search...
Proteomic databases
PaxDb	O48721.
PRIDE	O48721.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G26540.1; AT2G26540.1; AT2G26540.
GeneID	817195.
KEGG	ath:AT2G26540.
Organism-specific databases
TAIR	At2g26540.
Phylogenomic databases
eggNOG	COG1587.
HOGENOM	HOG000012769.
InParanoid	O48721.
KO	K01719.
OMA	TRERGKN.
PhylomeDB	O48721.
ProtClustDB	CLSN2688449.
Enzyme and pathway databases
BioCyc	MetaCyc:AT2G26540-MONOMER.
UniPathway	UPA00251; UER00320.
Gene expression databases
Genevestigator	O48721.
Family and domain databases
InterPro	IPR003754. 4pyrrol_synth_uPrphyn_synth. [Graphical view]
Pfam	PF02602. HEM4. 1 hit. [Graphical view]
SUPFAM	SSF69618. HEM4_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HEM4_ARATH

Accession

Primary (citable) accession number: O48721
Secondary accession number(s): Q8LBE8, Q8RVZ2, Q8VZ12

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program