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Protein

Uroporphyrinogen-III synthase, chloroplastic

Gene

UROS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, a precursor of tetrapyrroles such as chlorophyll, heme and phycobilins.1 Publication

Catalytic activityi

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Probable delta-aminolevulinic acid dehydratase 2, chloroplastic (HEMB2), Delta-aminolevulinic acid dehydratase 1, chloroplastic (HEMB1)
  2. Porphobilinogen deaminase, chloroplastic (HEMC)
  3. Uroporphyrinogen-III synthase, chloroplastic (UROS)
  4. Uroporphyrinogen decarboxylase 2, chloroplastic (HEME2), Uroporphyrinogen decarboxylase (AXX17_At3g15570), Uroporphyrinogen decarboxylase (At2g40490), Uroporphyrinogen decarboxylase (AXX17_At2g37600), Uroporphyrinogen decarboxylase 1, chloroplastic (HEME1)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

  • uroporphyrinogen-III synthase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processChlorophyll biosynthesis, Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciARA:AT2G26540-MONOMER.
MetaCyc:AT2G26540-MONOMER.
BRENDAi4.2.1.75. 399.
UniPathwayiUPA00251; UER00320.
UPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III synthase, chloroplastic (EC:4.2.1.75)
Short name:
AtUROS
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene namesi
Name:UROS
Synonyms:HEMD
Ordered Locus Names:At2g26540
ORF Names:T9J22.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G26540.
TAIRilocus:2066256. AT2G26540.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 81ChloroplastSequence analysisAdd BLAST81
ChainiPRO_000037606782 – 321Uroporphyrinogen-III synthase, chloroplasticAdd BLAST240

Proteomic databases

PaxDbiO48721.

PTM databases

iPTMnetiO48721.

Expressioni

Gene expression databases

ExpressionAtlasiO48721. baseline and differential.
GenevisibleiO48721. AT.

Interactioni

Protein-protein interaction databases

BioGridi2547. 1 interactor.
STRINGi3702.AT2G26540.1.

Structurei

3D structure databases

ProteinModelPortaliO48721.
SMRiO48721.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IFS1. Eukaryota.
ENOG4111P6K. LUCA.
HOGENOMiHOG000012769.
InParanoidiO48721.
KOiK01719.
OMAiACIGETS.
OrthoDBiEOG09360IGG.
PhylomeDBiO48721.

Family and domain databases

CDDicd06578. HemD. 1 hit.
InterProiView protein in InterPro
IPR036108. 4pyrrol_syn_uPrphyn_synt_sf.
IPR003754. 4pyrrol_synth_uPrphyn_synth.
PfamiView protein in Pfam
PF02602. HEM4. 1 hit.
SUPFAMiSSF69618. SSF69618. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O48721-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLLLSHCS ILSFQPPLSS SSSFHSSHIQ SLSKPVFASP SPIRNSISSS
60 70 80 90 100
VSSSSSSVSS SNSIPQVVVT RERGKNNQII KALEKNGISS LELPLIQHAR
110 120 130 140 150
GPDFDRLASV LNDKSFDWII ITSPEAGSVF LEAWKTASSP EVQIGVVGAG
160 170 180 190 200
TARVFEEAMK SADGLLHVAF TPSKATGKVL ASELPEKVGK RSSVLYPASL
210 220 230 240 250
KAGNDIVEGL SKRGFEVVRL NTYTTVPVQS VDTVLLQQAL SAPVLSVASP
260 270 280 290 300
SAVRAWLHLI QNEEQWSNYV ACIGETTASA ARRLGLKNVY YPEKPGLEGW
310 320
VESIMEALGA HADSSNPSSR N
Length:321
Mass (Da):34,225
Last modified:June 1, 2002 - v2
Checksum:i747E4E3C2DCEBD08
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29I → V in CAC85287 (PubMed:18042043).Curated1
Sequence conflicti218V → I in AAM64811 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ314569 mRNA. Translation: CAC85287.1.
AC002505 Genomic DNA. Translation: AAC14502.2.
CP002685 Genomic DNA. Translation: AEC07854.1.
BT026116 mRNA. Translation: ABG48472.1.
AY087255 mRNA. Translation: AAM64811.1.
AY065389 mRNA. Translation: AAL38830.2.
PIRiT00987.
RefSeqiNP_565625.1. NM_128211.4.
UniGeneiAt.28505.

Genome annotation databases

EnsemblPlantsiAT2G26540.1; AT2G26540.1; AT2G26540.
GeneIDi817195.
GrameneiAT2G26540.1; AT2G26540.1; AT2G26540.
KEGGiath:AT2G26540.

Similar proteinsi

Entry informationi

Entry nameiHEM4_ARATH
AccessioniPrimary (citable) accession number: O48721
Secondary accession number(s): Q8LBE8, Q8RVZ2, Q8VZ12
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: June 1, 2002
Last modified: October 25, 2017
This is version 97 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families