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O48721

- HEM4_ARATH

UniProt

O48721 - HEM4_ARATH

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Protein

Uroporphyrinogen-III synthase, chloroplastic

Gene

UROS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, a precursor of tetrapyrroles such as chlorophyll, heme and phycobilins.1 Publication

Catalytic activityi

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathwayi

GO - Molecular functioni

  1. uroporphyrinogen-III synthase activity Source: UniProtKB

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB-UniPathway
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
  3. uroporphyrinogen III biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Chlorophyll biosynthesis, Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciARA:AT2G26540-MONOMER.
MetaCyc:AT2G26540-MONOMER.
UniPathwayiUPA00251; UER00320.
UPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III synthase, chloroplastic (EC:4.2.1.75)
Short name:
AtUROS
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene namesi
Name:UROS
Synonyms:HEMD
Ordered Locus Names:At2g26540
ORF Names:T9J22.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G26540.

Subcellular locationi

Plastidchloroplast 1 Publication

GO - Cellular componenti

  1. chloroplast Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8181ChloroplastSequence AnalysisAdd
BLAST
Chaini82 – 321240Uroporphyrinogen-III synthase, chloroplasticPRO_0000376067Add
BLAST

Proteomic databases

PaxDbiO48721.
PRIDEiO48721.

Expressioni

Gene expression databases

GenevestigatoriO48721.

Interactioni

Protein-protein interaction databases

BioGridi2547. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO48721.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1587.
HOGENOMiHOG000012769.
InParanoidiO48721.
KOiK01719.
OMAiWIIITSP.
PhylomeDBiO48721.

Family and domain databases

InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMiSSF69618. SSF69618. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O48721-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALLLLSHCS ILSFQPPLSS SSSFHSSHIQ SLSKPVFASP SPIRNSISSS
60 70 80 90 100
VSSSSSSVSS SNSIPQVVVT RERGKNNQII KALEKNGISS LELPLIQHAR
110 120 130 140 150
GPDFDRLASV LNDKSFDWII ITSPEAGSVF LEAWKTASSP EVQIGVVGAG
160 170 180 190 200
TARVFEEAMK SADGLLHVAF TPSKATGKVL ASELPEKVGK RSSVLYPASL
210 220 230 240 250
KAGNDIVEGL SKRGFEVVRL NTYTTVPVQS VDTVLLQQAL SAPVLSVASP
260 270 280 290 300
SAVRAWLHLI QNEEQWSNYV ACIGETTASA ARRLGLKNVY YPEKPGLEGW
310 320
VESIMEALGA HADSSNPSSR N
Length:321
Mass (Da):34,225
Last modified:June 1, 2002 - v2
Checksum:i747E4E3C2DCEBD08
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291I → V in CAC85287. (PubMed:18042043)Curated
Sequence conflicti218 – 2181V → I in AAM64811. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ314569 mRNA. Translation: CAC85287.1.
AC002505 Genomic DNA. Translation: AAC14502.2.
CP002685 Genomic DNA. Translation: AEC07854.1.
BT026116 mRNA. Translation: ABG48472.1.
AY087255 mRNA. Translation: AAM64811.1.
AY065389 mRNA. Translation: AAL38830.2.
PIRiT00987.
RefSeqiNP_565625.1. NM_128211.3.
UniGeneiAt.28505.

Genome annotation databases

EnsemblPlantsiAT2G26540.1; AT2G26540.1; AT2G26540.
GeneIDi817195.
KEGGiath:AT2G26540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ314569 mRNA. Translation: CAC85287.1 .
AC002505 Genomic DNA. Translation: AAC14502.2 .
CP002685 Genomic DNA. Translation: AEC07854.1 .
BT026116 mRNA. Translation: ABG48472.1 .
AY087255 mRNA. Translation: AAM64811.1 .
AY065389 mRNA. Translation: AAL38830.2 .
PIRi T00987.
RefSeqi NP_565625.1. NM_128211.3.
UniGenei At.28505.

3D structure databases

ProteinModelPortali O48721.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 2547. 1 interaction.

Proteomic databases

PaxDbi O48721.
PRIDEi O48721.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G26540.1 ; AT2G26540.1 ; AT2G26540 .
GeneIDi 817195.
KEGGi ath:AT2G26540.

Organism-specific databases

TAIRi AT2G26540.

Phylogenomic databases

eggNOGi COG1587.
HOGENOMi HOG000012769.
InParanoidi O48721.
KOi K01719.
OMAi WIIITSP.
PhylomeDBi O48721.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00320 .
UPA00668 .
BioCyci ARA:AT2G26540-MONOMER.
MetaCyc:AT2G26540-MONOMER.

Gene expression databases

Genevestigatori O48721.

Family and domain databases

InterProi IPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view ]
Pfami PF02602. HEM4. 1 hit.
[Graphical view ]
SUPFAMi SSF69618. SSF69618. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of the Arabidopsis gene encoding the tetrapyrrole biosynthesis enzyme uroporphyrinogen III synthase."
    Tan F.-C., Cheng Q., Saha K., Heinemann I.U., Jahn M., Jahn D., Smith A.G.
    Biochem. J. 410:291-299(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 132-321.
    Strain: cv. Columbia.

Entry informationi

Entry nameiHEM4_ARATH
AccessioniPrimary (citable) accession number: O48721
Secondary accession number(s): Q8LBE8, Q8RVZ2, Q8VZ12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: June 1, 2002
Last modified: October 1, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3