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Protein

Uroporphyrinogen-III synthase, chloroplastic

Gene

UROS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III, a precursor of tetrapyrroles such as chlorophyll, heme and phycobilins.1 Publication

Catalytic activityi

Hydroxymethylbilane = uroporphyrinogen III + H2O.

Pathway: protoporphyrin-IX biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Probable delta-aminolevulinic acid dehydratase 2, chloroplastic (HEMB2), Delta-aminolevulinic acid dehydratase 1, chloroplastic (HEMB1)
  2. Porphobilinogen deaminase, chloroplastic (HEMC)
  3. Uroporphyrinogen-III synthase, chloroplastic (UROS)
  4. Uroporphyrinogen decarboxylase 2, chloroplastic (HEME2), Uroporphyrinogen decarboxylase (At2g40490), Uroporphyrinogen decarboxylase 1, chloroplastic (HEME1)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Pathway: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

  • uroporphyrinogen-III synthase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Chlorophyll biosynthesis, Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciARA:AT2G26540-MONOMER.
MetaCyc:AT2G26540-MONOMER.
BRENDAi4.2.1.75. 399.
UniPathwayiUPA00251; UER00320.
UPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III synthase, chloroplastic (EC:4.2.1.75)
Short name:
AtUROS
Alternative name(s):
Hydroxymethylbilane hydrolyase [cyclizing]
Uroporphyrinogen-III cosynthase
Gene namesi
Name:UROS
Synonyms:HEMD
Ordered Locus Names:At2g26540
ORF Names:T9J22.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G26540.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8181ChloroplastSequence AnalysisAdd
BLAST
Chaini82 – 321240Uroporphyrinogen-III synthase, chloroplasticPRO_0000376067Add
BLAST

Proteomic databases

PaxDbiO48721.
PRIDEiO48721.

Interactioni

Protein-protein interaction databases

BioGridi2547. 1 interaction.
STRINGi3702.AT2G26540.1.

Structurei

3D structure databases

ProteinModelPortaliO48721.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1587.
HOGENOMiHOG000012769.
InParanoidiO48721.
KOiK01719.
OMAiWIIITSP.
PhylomeDBiO48721.

Family and domain databases

InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMiSSF69618. SSF69618. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O48721-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALLLLSHCS ILSFQPPLSS SSSFHSSHIQ SLSKPVFASP SPIRNSISSS
60 70 80 90 100
VSSSSSSVSS SNSIPQVVVT RERGKNNQII KALEKNGISS LELPLIQHAR
110 120 130 140 150
GPDFDRLASV LNDKSFDWII ITSPEAGSVF LEAWKTASSP EVQIGVVGAG
160 170 180 190 200
TARVFEEAMK SADGLLHVAF TPSKATGKVL ASELPEKVGK RSSVLYPASL
210 220 230 240 250
KAGNDIVEGL SKRGFEVVRL NTYTTVPVQS VDTVLLQQAL SAPVLSVASP
260 270 280 290 300
SAVRAWLHLI QNEEQWSNYV ACIGETTASA ARRLGLKNVY YPEKPGLEGW
310 320
VESIMEALGA HADSSNPSSR N
Length:321
Mass (Da):34,225
Last modified:June 1, 2002 - v2
Checksum:i747E4E3C2DCEBD08
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291I → V in CAC85287 (PubMed:18042043).Curated
Sequence conflicti218 – 2181V → I in AAM64811 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ314569 mRNA. Translation: CAC85287.1.
AC002505 Genomic DNA. Translation: AAC14502.2.
CP002685 Genomic DNA. Translation: AEC07854.1.
BT026116 mRNA. Translation: ABG48472.1.
AY087255 mRNA. Translation: AAM64811.1.
AY065389 mRNA. Translation: AAL38830.2.
PIRiT00987.
RefSeqiNP_565625.1. NM_128211.3.
UniGeneiAt.28505.

Genome annotation databases

EnsemblPlantsiAT2G26540.1; AT2G26540.1; AT2G26540.
GeneIDi817195.
KEGGiath:AT2G26540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ314569 mRNA. Translation: CAC85287.1.
AC002505 Genomic DNA. Translation: AAC14502.2.
CP002685 Genomic DNA. Translation: AEC07854.1.
BT026116 mRNA. Translation: ABG48472.1.
AY087255 mRNA. Translation: AAM64811.1.
AY065389 mRNA. Translation: AAL38830.2.
PIRiT00987.
RefSeqiNP_565625.1. NM_128211.3.
UniGeneiAt.28505.

3D structure databases

ProteinModelPortaliO48721.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2547. 1 interaction.
STRINGi3702.AT2G26540.1.

Proteomic databases

PaxDbiO48721.
PRIDEiO48721.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G26540.1; AT2G26540.1; AT2G26540.
GeneIDi817195.
KEGGiath:AT2G26540.

Organism-specific databases

TAIRiAT2G26540.

Phylogenomic databases

eggNOGiCOG1587.
HOGENOMiHOG000012769.
InParanoidiO48721.
KOiK01719.
OMAiWIIITSP.
PhylomeDBiO48721.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00320.
UPA00668.
BioCyciARA:AT2G26540-MONOMER.
MetaCyc:AT2G26540-MONOMER.
BRENDAi4.2.1.75. 399.

Miscellaneous databases

PROiO48721.

Family and domain databases

InterProiIPR003754. 4pyrrol_synth_uPrphyn_synth.
[Graphical view]
PfamiPF02602. HEM4. 1 hit.
[Graphical view]
SUPFAMiSSF69618. SSF69618. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of the Arabidopsis gene encoding the tetrapyrrole biosynthesis enzyme uroporphyrinogen III synthase."
    Tan F.-C., Cheng Q., Saha K., Heinemann I.U., Jahn M., Jahn D., Smith A.G.
    Biochem. J. 410:291-299(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 132-321.
    Strain: cv. Columbia.

Entry informationi

Entry nameiHEM4_ARATH
AccessioniPrimary (citable) accession number: O48721
Secondary accession number(s): Q8LBE8, Q8RVZ2, Q8VZ12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: June 1, 2002
Last modified: June 24, 2015
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.