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Protein

Probable pectinesterase/pectinesterase inhibitor 12

Gene

PME12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin.By similarity

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Probable pectinesterase 48 (PME48), Probable pectinesterase 49 (PME49), Probable pectinesterase 50 (PME50), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Probable pectinesterase 15 (PME15), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Putative pectinesterase 14 (PME14), Pectinesterase (At3g10720), Probable pectinesterase 55 (PME55), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Pectinesterase 4 (PME4), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase (At3g14310), Pectinesterase/pectinesterase inhibitor 18 (PME18), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Pectinesterase 1 (PME1), Putative pectinesterase 63 (PME63), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Putative pectinesterase 10 (PME10), Pectinesterase (F14I3.7), Pectinesterase 2 (PME2), Probable pectinesterase 29 (PME29), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Probable pectinesterase 8 (PME8), Pectinesterase, Pectinesterase/pectinesterase inhibitor 3 (PME3), Pectinesterase 31 (PME31), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Putative pectinesterase 57 (PME57), Pectinesterase (At3g62170), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Pectinesterase (T27B3.30), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Pectinesterase QRT1 (QRT1), Putative pectinesterase 11 (PME11), Pectinesterase PPME1 (PPME1), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Putative pectinesterase 52 (PME52), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Pectinesterase (AXX17_AT1G48300), Pectinesterase (At1g53840), Pectinesterase 5 (PME5), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Probable pectinesterase 30 (PME30), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Probable pectinesterase 68 (PME68), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Pectinesterase, Probable pectinesterase 67 (PME67), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Probable pectinesterase 56 (PME56), Pectinesterase, Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Probable pectinesterase 53 (PME53), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Probable pectinesterase 66 (PME66), Probable pectinesterase/pectinesterase inhibitor 6 (PME6)
  2. Pectate lyase (T26I12.20), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 18 (At4g24780), Probable pectate lyase 16 (At4g22080), Putative pectate lyase 17 (At4g22090), Putative pectate lyase 14 (At4g13210), Pectate lyase (At3g55140), Probable pectate lyase 20 (At5g48900), Probable pectate lyase 6 (At2g02720), Pectate lyase (At1g14420), Pectate lyase (At3g01270), Pectate lyase (At2g02720), Probable pectate lyase 22 (At5g63180), Pectate lyase (T5E8_80), Pectate lyase (At3g01270), Pectate lyase (At3g55140), Putative pectate lyase 2 (At1g11920), Pectate lyase (At3g07010), Pectate lyase, Probable pectate lyase 8 (At3g07010), Probable pectate lyase 4 (At1g30350), Probable pectate lyase 19 (At5g15110), Pectate lyase (F11F8_12), Probable pectate lyase 13 (PMR6), Probable pectate lyase 3 (AT59), Probable pectate lyase 5 (At1g67750), Putative pectate lyase 21 (At5g55720), Pectate lyase (At4g13210), Probable pectate lyase 12 (At3g53190), Pectate lyase (At4g24780), Pectate lyase (At5g04310), Pectate lyase (At4g13710), Probable pectate lyase 15 (At4g13710), Pectate lyase (At3g53190), Pectate lyase (At3g01270), Putative pectate lyase 11 (At3g27400), Pectate lyase (At5g04310), Pectate lyase (At3g07010), Probable pectate lyase 9 (At3g24230), Probable pectate lyase 1 (At1g04680), Probable pectate lyase 10 (At3g24670)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei312 – 3121Substrate; for pectinesterase activityBy similarity
Binding sitei342 – 3421Substrate; for pectinesterase activityBy similarity
Sitei364 – 3641Transition state stabilizerBy similarity
Active sitei365 – 3651Proton donor; for pectinesterase activityPROSITE-ProRule annotation
Active sitei386 – 3861Nucleophile; for pectinesterase activityPROSITE-ProRule annotation
Binding sitei454 – 4541Substrate; for pectinesterase activityBy similarity
Binding sitei456 – 4561Substrate; for pectinesterase activityBy similarity

GO - Molecular functioni

GO - Biological processi

  • cell wall modification Source: InterPro
  • pectin catabolic process Source: UniProtKB-UniPathway
  • response to bacterium Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT2G26440-MONOMER.
UniPathwayiUPA00545; UER00823.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pectinesterase/pectinesterase inhibitor 12
Including the following 2 domains:
Pectinesterase inhibitor 12
Alternative name(s):
Pectin methylesterase inhibitor 12
Pectinesterase 12 (EC:3.1.1.11)
Short name:
PE 12
Alternative name(s):
Pectin methylesterase 12
Short name:
AtPME12
Gene namesi
Name:PME12
Synonyms:ARATH12
Ordered Locus Names:At2g26440
ORF Names:T9J22.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G26440.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 547524Probable pectinesterase/pectinesterase inhibitor 12PRO_0000371669Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi131 – 1311N-linked (GlcNAc...)Sequence analysis
Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence analysis
Glycosylationi260 – 2601N-linked (GlcNAc...)Sequence analysis
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence analysis
Disulfide bondi379 ↔ 399By similarity
Glycosylationi432 – 4321N-linked (GlcNAc...)Sequence analysis
Glycosylationi443 – 4431N-linked (GlcNAc...)Sequence analysis
Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO48711.
PRIDEiO48711.

Expressioni

Tissue specificityi

Expressed in siliques.1 Publication

Developmental stagei

Expressed throughout silique development.1 Publication

Gene expression databases

GenevisibleiO48711. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G26440.1.

Structurei

3D structure databases

ProteinModelPortaliO48711.
SMRiO48711. Positions 239-547.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni31 – 185155Pectinesterase inhibitor 12Add
BLAST
Regioni237 – 533297Pectinesterase 12Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IEPW. Eukaryota.
COG4677. LUCA.
HOGENOMiHOG000217409.
InParanoidiO48711.
OMAiIYKTNIV.
OrthoDBiEOG09360CYZ.
PhylomeDBiO48711.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O48711-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSSFNLSS LLFLLFFTPS VFSYSYQPSL NPHETSATSF CKNTPYPDAC
60 70 80 90 100
FTSLKLSISI NISPNILSFL LQTLQTALSE AGKLTDLLSG AGVSNNLVEG
110 120 130 140 150
QRGSLQDCKD LHHITSSFLK RSISKIQDGV NDSRKLADAR AYLSAALTNK
160 170 180 190 200
ITCLEGLESA SGPLKPKLVT SFTTTYKHIS NSLSALPKQR RTTNPKTGGN
210 220 230 240 250
TKNRRLLGLF PDWVYKKDHR FLEDSSDGYD EYDPSESLVV AADGTGNFST
260 270 280 290 300
INEAISFAPN MSNDRVLIYV KEGVYDENID IPIYKTNIVL IGDGSDVTFI
310 320 330 340 350
TGNRSVGDGW TTFRSATLAV SGEGFLARDI MITNTAGPEK HQAVALRVNA
360 370 380 390 400
DFVALYRCVI DGYQDTLYTH SFRQFYRECD IYGTIDYIFG NAAVVFQGCN
410 420 430 440 450
IVSKLPMPGQ FTVITAQSRD TQDEDTGISM QNCSILASED LFNSSNKVKS
460 470 480 490 500
YLGRPWREFS RTVVMESYID EFIDGSGWSK WNGGEALDTL YYGEYNNNGP
510 520 530 540
GSETVKRVNW PGFHIMGYED AFNFTATEFI TGDGWLGSTS FPYDNGI
Length:547
Mass (Da):60,417
Last modified:June 1, 1998 - v1
Checksum:i9CEB99E60E6378C7
GO

Sequence cautioni

The sequence BAD93990 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002505 Genomic DNA. Translation: AAC14493.1.
CP002685 Genomic DNA. Translation: AEC07839.1.
AY072224 mRNA. Translation: AAL60045.1.
AY122952 mRNA. Translation: AAM67485.1.
AK220775 mRNA. Translation: BAD93990.1. Different initiation.
PIRiT00977.
RefSeqiNP_180212.1. NM_128201.4.
UniGeneiAt.38895.

Genome annotation databases

EnsemblPlantsiAT2G26440.1; AT2G26440.1; AT2G26440.
GeneIDi817184.
GrameneiAT2G26440.1; AT2G26440.1; AT2G26440.
KEGGiath:AT2G26440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002505 Genomic DNA. Translation: AAC14493.1.
CP002685 Genomic DNA. Translation: AEC07839.1.
AY072224 mRNA. Translation: AAL60045.1.
AY122952 mRNA. Translation: AAM67485.1.
AK220775 mRNA. Translation: BAD93990.1. Different initiation.
PIRiT00977.
RefSeqiNP_180212.1. NM_128201.4.
UniGeneiAt.38895.

3D structure databases

ProteinModelPortaliO48711.
SMRiO48711. Positions 239-547.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G26440.1.

Proteomic databases

PaxDbiO48711.
PRIDEiO48711.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G26440.1; AT2G26440.1; AT2G26440.
GeneIDi817184.
GrameneiAT2G26440.1; AT2G26440.1; AT2G26440.
KEGGiath:AT2G26440.

Organism-specific databases

TAIRiAT2G26440.

Phylogenomic databases

eggNOGiENOG410IEPW. Eukaryota.
COG4677. LUCA.
HOGENOMiHOG000217409.
InParanoidiO48711.
OMAiIYKTNIV.
OrthoDBiEOG09360CYZ.
PhylomeDBiO48711.

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.
BioCyciARA:AT2G26440-MONOMER.

Miscellaneous databases

PROiO48711.

Gene expression databases

GenevisibleiO48711. AT.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
IPR018040. Pectinesterase_Tyr_AS.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00800. PECTINESTERASE_1. 1 hit.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPME12_ARATH
AccessioniPrimary (citable) accession number: O48711
Secondary accession number(s): Q570D4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: June 1, 1998
Last modified: September 7, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.