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Protein

(6-4)DNA photolyase

Gene

UVR3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in repair of UV radiation-induced DNA damage. Catalyzes the photoreactivation of pyrimidine [6-4] pyrimidone photoproduct (6-4 products). Binds specifically to DNA containing 6-4 products and repairs these lesions in a visible light-dependent manner. Not required for repair of cyclobutane pyrimidine dimer (CPD).2 Publications

Catalytic activityi

6-4 photoproduct (in DNA) = 2 pyrimidine residues (in DNA).

Cofactori

FAD1 PublicationNote: Binds 1 FAD per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei262 – 2621Phosphate; via amide nitrogen and carbonyl oxygen
Binding sitei263 – 2631FAD1 Publication
Binding sitei320 – 3201DNABy similarity
Sitei348 – 3481Electron transfer via tryptophanyl radicalBy similarity
Binding sitei386 – 3861FAD1 Publication
Sitei402 – 4021Electron transfer via tryptophanyl radicalBy similarity
Binding sitei421 – 4211FAD1 Publication
Sitei425 – 4251Electron transfer via tryptophanyl radicalBy similarity
Binding sitei427 – 4271DNABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi276 – 2805FAD1 Publication
Nucleotide bindingi317 – 3215FAD1 Publication
Nucleotide bindingi380 – 3834FAD1 Publication
Nucleotide bindingi415 – 4173FAD1 Publication

GO - Molecular functioni

  • DNA (6-4) photolyase activity Source: TAIR
  • DNA binding Source: UniProtKB-KW
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  • DNA repair Source: UniProtKB-KW
  • response to UV Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, FAD, Flavoprotein, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:GQT-2758-MONOMER.
ARA:GQT-2759-MONOMER.
MetaCyc:MONOMER-15021.
BRENDAi4.1.99.13. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
(6-4)DNA photolyase (EC:4.1.99.13)
Alternative name(s):
Protein UV repair defective 3
Gene namesi
Name:UVR3
Ordered Locus Names:At3g15620
ORF Names:SJ11.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G15620.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi263 – 2631K → R: No effect on DNA repair activity. 1 Publication
Mutagenesisi276 – 2761T → P: No effect on DNA repair activity. 1 Publication
Mutagenesisi383 – 3831H → A: Loss of DNA repair activity. 1 Publication
Mutagenesisi387 – 3871H → A: Loss of DNA repair activity. 1 Publication
Mutagenesisi439 – 4391R → H or A: No effect on DNA repair activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556(6-4)DNA photolyasePRO_0000397886Add
BLAST

Proteomic databases

PaxDbiO48652.
PRIDEiO48652.

Expressioni

Tissue specificityi

Expressed in siliques, flowers and leaves. Not detected in roots.1 Publication

Developmental stagei

Expressed at all developmental stages.1 Publication

Inductioni

Not induced by white or UV-B light.1 Publication

Gene expression databases

GenevisibleiO48652. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G15620.1.

Structurei

Secondary structure

1
556
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi25 – 328Combined sources
Helixi40 – 467Combined sources
Beta strandi52 – 587Combined sources
Helixi60 – 634Combined sources
Beta strandi69 – 735Combined sources
Helixi79 – 9820Combined sources
Beta strandi104 – 1085Combined sources
Helixi110 – 1189Combined sources
Beta strandi123 – 1286Combined sources
Helixi134 – 14916Combined sources
Beta strandi153 – 1553Combined sources
Beta strandi160 – 1634Combined sources
Helixi165 – 1717Combined sources
Beta strandi173 – 1753Combined sources
Helixi180 – 1878Combined sources
Turni191 – 1944Combined sources
Turni221 – 2255Combined sources
Helixi228 – 2303Combined sources
Helixi241 – 25111Combined sources
Helixi255 – 2595Combined sources
Helixi263 – 2653Combined sources
Helixi280 – 2845Combined sources
Helixi290 – 30213Combined sources
Turni310 – 3123Combined sources
Helixi314 – 32916Combined sources
Turni332 – 3354Combined sources
Helixi352 – 3598Combined sources
Helixi366 – 37813Combined sources
Helixi383 – 39311Combined sources
Turni394 – 3985Combined sources
Helixi402 – 41211Combined sources
Helixi418 – 42811Combined sources
Beta strandi431 – 4333Combined sources
Turni443 – 4464Combined sources
Helixi447 – 4493Combined sources
Helixi455 – 4606Combined sources
Helixi462 – 4643Combined sources
Turni469 – 4735Combined sources
Helixi475 – 4773Combined sources
Helixi480 – 4867Combined sources
Turni490 – 4923Combined sources
Helixi501 – 52121Combined sources
Turni522 – 5243Combined sources
Helixi528 – 54114Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FY4X-ray2.70A/B/C20-556[»]
ProteinModelPortaliO48652.
SMRiO48652. Positions 23-544.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO48652.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 162139Photolyase/cryptochrome alpha/betaAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni382 – 3876Interaction with DNABy similarity

Sequence similaritiesi

Belongs to the DNA photolyase class-1 family.Curated

Phylogenomic databases

eggNOGiKOG0133. Eukaryota.
COG0415. LUCA.
HOGENOMiHOG000245622.
InParanoidiO48652.
KOiK02295.
OMAiGIPNFNK.
OrthoDBiEOG0936062H.
PhylomeDBiO48652.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR005101. Cryptochr/Photolyase_FAD-bd.
IPR006050. DNA_photolyase_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00875. DNA_photolyase. 1 hit.
PF03441. FAD_binding_7. 1 hit.
[Graphical view]
SUPFAMiSSF48173. SSF48173. 1 hit.
SSF52425. SSF52425. 1 hit.
PROSITEiPS51645. PHR_CRY_ALPHA_BETA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O48652-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQRFCVCSPS SYRLNPITSM ATGSGSLIWF RKGLRVHDNP ALEYASKGSE
60 70 80 90 100
FMYPVFVIDP HYMESDPSAF SPGSSRAGVN RIRFLLESLK DLDSSLKKLG
110 120 130 140 150
SRLLVFKGEP GEVLVRCLQE WKVKRLCFEY DTDPYYQALD VKVKDYASST
160 170 180 190 200
GVEVFSPVSH TLFNPAHIIE KNGGKPPLSY QSFLKVAGEP SCAKSELVMS
210 220 230 240 250
YSSLPPIGDI GNLGISEVPS LEELGYKDDE QADWTPFRGG ESEALKRLTK
260 270 280 290 300
SISDKAWVAN FEKPKGDPSA FLKPATTVMS PYLKFGCLSS RYFYQCLQNI
310 320 330 340 350
YKDVKKHTSP PVSLLGQLLW REFFYTTAFG TPNFDKMKGN RICKQIPWNE
360 370 380 390 400
DHAMLAAWRD GKTGYPWIDA IMVQLLKWGW MHHLARHCVA CFLTRGDLFI
410 420 430 440 450
HWEQGRDVFE RLLIDSDWAI NNGNWMWLSC SSFFYQFNRI YSPISFGKKY
460 470 480 490 500
DPDGKYIRHF LPVLKDMPKQ YIYEPWTAPL SVQTKANCIV GKDYPKPMVL
510 520 530 540 550
HDSASKECKR KMGEAYALNK KMDGKVDEEN LRDLRRKLQK DEHEESKIRN

QRPKLK
Length:556
Mass (Da):63,790
Last modified:October 5, 2010 - v2
Checksum:i3110841DBA33CAC8
GO
Isoform 2 (identifier: O48652-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     437-445: FNRIYSPIS → ALSPFCFSF
     446-556: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:445
Mass (Da):50,609
Checksum:iC3881E2AF621661B
GO

Sequence cautioni

The sequence BAA24449 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA34711 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei437 – 4459FNRIYSPIS → ALSPFCFSF in isoform 2. CuratedVSP_039720
Alternative sequencei446 – 556111Missing in isoform 2. CuratedVSP_039721Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003687 mRNA. Translation: BAA24449.1. Different initiation.
AB017331 Genomic DNA. Translation: BAA34711.1. Different initiation.
AB017071 Genomic DNA. Translation: BAB02293.1.
CP002686 Genomic DNA. Translation: AEE75703.1.
CP002686 Genomic DNA. Translation: AEE75704.1.
RefSeqiNP_001030703.1. NM_001035626.1. [O48652-2]
NP_566520.1. NM_112432.1. [O48652-1]
UniGeneiAt.188.

Genome annotation databases

EnsemblPlantsiAT3G15620.1; AT3G15620.1; AT3G15620. [O48652-1]
GeneIDi820804.
KEGGiath:AT3G15620.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003687 mRNA. Translation: BAA24449.1. Different initiation.
AB017331 Genomic DNA. Translation: BAA34711.1. Different initiation.
AB017071 Genomic DNA. Translation: BAB02293.1.
CP002686 Genomic DNA. Translation: AEE75703.1.
CP002686 Genomic DNA. Translation: AEE75704.1.
RefSeqiNP_001030703.1. NM_001035626.1. [O48652-2]
NP_566520.1. NM_112432.1. [O48652-1]
UniGeneiAt.188.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FY4X-ray2.70A/B/C20-556[»]
ProteinModelPortaliO48652.
SMRiO48652. Positions 23-544.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G15620.1.

Proteomic databases

PaxDbiO48652.
PRIDEiO48652.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G15620.1; AT3G15620.1; AT3G15620. [O48652-1]
GeneIDi820804.
KEGGiath:AT3G15620.

Organism-specific databases

TAIRiAT3G15620.

Phylogenomic databases

eggNOGiKOG0133. Eukaryota.
COG0415. LUCA.
HOGENOMiHOG000245622.
InParanoidiO48652.
KOiK02295.
OMAiGIPNFNK.
OrthoDBiEOG0936062H.
PhylomeDBiO48652.

Enzyme and pathway databases

BioCyciARA:GQT-2758-MONOMER.
ARA:GQT-2759-MONOMER.
MetaCyc:MONOMER-15021.
BRENDAi4.1.99.13. 399.

Miscellaneous databases

EvolutionaryTraceiO48652.
PROiO48652.

Gene expression databases

GenevisibleiO48652. AT.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR005101. Cryptochr/Photolyase_FAD-bd.
IPR006050. DNA_photolyase_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00875. DNA_photolyase. 1 hit.
PF03441. FAD_binding_7. 1 hit.
[Graphical view]
SUPFAMiSSF48173. SSF48173. 1 hit.
SSF52425. SSF52425. 1 hit.
PROSITEiPS51645. PHR_CRY_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUVR3_ARATH
AccessioniPrimary (citable) accession number: O48652
Secondary accession number(s): Q2V3V6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 5, 2010
Last modified: September 7, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.