Reviewed, UniProtKB/Swiss-Prot O48604 (HPPD_HORVU)

Names and origin

Protein names	Recommended name:     4-hydroxyphenylpyruvate dioxygenase     EC=1.13.11.27 Alternative name(s):     4-hydroxyphenylpyruvic acid oxidase       Short name=HPPDase       Short name=4HPPD       Short name=HPD
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	4-hydroxyphenylpyruvate + O(2) = homogentisate + CO(2).
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 3/6. Cofactor biosynthesis; prenylquinone biosynthesis.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the 4HPPD family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-hydroxyphenylpyruvate dioxygenase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	4-hydroxyphenylpyruvate dioxygenase		PRO_0000088399
Sites
	Metal binding	211	1	Iron By similarity
	Metal binding	293	1	Iron By similarity
	Metal binding	379	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	O48604-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 42DE721182654A4C	FASTA	434	46,550
	10 20 30 40 50 60 MPPTPTTPAA TGAAAAVTPE HARPHRMVRF NPRSDRFHTL SFHHVEFWCA DAASAAGRFA 70 80 90 100 110 120 FALGAPLAAR SDLSTGNSAH ASQLLRSGSL AFLFTAPYAN GCDAATASLP SFSADAARRF 130 140 150 160 170 180 SADHGIAVRS VALRVADAAE AFRASRRRGA RPAFAPVDLG RGFAFAEVEL YGDVVLRFVS 190 200 210 220 230 240 HPDGTDVPFL PGFEGVTNPD AVDYGLTRFD HVVGNVPELA PAAAYIAGFT GFHEFAEFTA 250 260 270 280 290 300 EDVGTTESGL NSVVLANNSE GVLLPLNEPV HGTKRRSQIQ TFLEHHGGPG VQHIAVASSD 310 320 330 340 350 360 VLRTLRKMRA RSAMGGFDFL PPPLPKYYEG VRRLAGDVLS EAQIKECQEL GVLVDRDDQG 370 380 390 400 410 420 VLLQIFTKPV GDRPTLFLEM IQRIGCMEKD ERGEEYQKGG CGGFGKGNFS ELFKSIEDYE 430 KSLEAKQSAA VQGS

References

[1]

"cDNA sequence encoding a barley 4-hydroxyphenylpyruvate dioxygenase with enhanced expression in meristematic leaf tissue and during leaf senescence." Krupinska K., Falk J. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Carina. Tissue: Meristem.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ000693 mRNA. Translation: CAA04245.1.
PIR	T04471.
3D structure databases
SMR	O48604. Positions 28-424.
ModBase	Search...
Organism-specific databases
Gramene	O48604.
Family and domain databases
InterPro	IPR005956. 4OHPhenylPyrv_dOase. IPR004360. Glyas_bleo-R_dOase. [Graphical view]
PANTHER	PTHR11959. HPP_dOase. 1 hit.
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
PIRSF	PIRSF009283. HPP_dOase. 1 hit.
TIGRFAMs	TIGR01263. 4HPPD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPD_HORVU

Accession

Primary (citable) accession number: O48604

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	June 1, 1998
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)