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Protein

Asparagine--tRNA ligase, chloroplastic/mitochondrial

Gene

SYNO

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn).Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi113 – 191OBSequence analysisAdd BLAST79

GO - Molecular functioni

  • asparagine-tRNA ligase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW

GO - Biological processi

  • asparaginyl-tRNA aminoacylation Source: TAIR
  • plant ovule development Source: TAIR

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, DNA-binding, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G17300-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine--tRNA ligase, chloroplastic/mitochondrialCurated (EC:6.1.1.22Curated)
Alternative name(s):
Asparaginyl-tRNA synthetaseCurated
Short name:
AsnRSCurated
AtNS11 Publication
Protein OVULE ABORTION 81 Publication
Gene namesi
Name:SYNO1 Publication
Synonyms:NS11 Publication, OVA81 Publication
Ordered Locus Names:At4g17300
ORF Names:dl4685w
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G17300.
TAIRilocus:2130804. AT4G17300.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Lethal. In heterozygous plants, aborted ovules.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000035800? – 567Asparagine--tRNA ligase, chloroplastic/mitochondrial
Transit peptidei1 – ?Chloroplast and mitochondrionSequence analysis

Proteomic databases

PaxDbiO48593.
PRIDEiO48593.

Expressioni

Gene expression databases

GenevisibleiO48593. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G17300.1.

Structurei

3D structure databases

ProteinModelPortaliO48593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0554. Eukaryota.
COG0017. LUCA.
HOGENOMiHOG000226033.
InParanoidiO48593.
KOiK01893.
OMAiFFHISKP.
OrthoDBiEOG09360702.
PhylomeDBiO48593.

Family and domain databases

HAMAPiMF_00534. Asn_tRNA_synth. 1 hit.
InterProiView protein in InterPro
IPR004364. aa-tRNA-synt_II.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
PANTHERiPTHR22594. PTHR22594. 1 hit.
PfamiView protein in Pfam
PF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
PRINTSiPR01042. TRNASYNTHASP.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00457. asnS. 1 hit.
PROSITEiView protein in PROSITE
PS50862. AA_TRNA_LIGASE_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O48593-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATFLPATS LRLTQNSTLR FLSFFTISNP SYSLFRPLRR RVLPPFDAFP
60 70 80 90 100
ANSRRRCFCT AVSESLGSGD GNKVESYEKR FGSKVGEFRK KLRIAEVKGG
110 120 130 140 150
ADEGLSRVGQ SLNIMGWVRT LRSQSSVTFI EINDGSCLSN LQCVMTSDAE
160 170 180 190 200
GYDQVESGSI LTGASVSVQG TIVASQGTKQ KVELKVEKII VVGECDSSYP
210 220 230 240 250
IQKKRVSREF LRTKAHLRPR TNTFGAVARV RNTLAYATHK FFQESGFVWV
260 270 280 290 300
ASPIITASDC EGAGEQFCVT TLIPSSHENT DTSIDAIPKT KGGLIDWSQD
310 320 330 340 350
FFGKPAFLTV SGQLNGETYA TALSDVYTFG PTFRAENSNT SRHLAEFWMI
360 370 380 390 400
EPELAFADLD DDMACATAYL QYVVKYVLDN CKEDMEFFDT WIEKGIIRRL
410 420 430 440 450
SDVAEKEFLQ LGYTDAIEIL LKANKKFDFP VKWGLDLQSE HERYITEEAF
460 470 480 490 500
GGRPVIIRDY PKEIKAFYMR ENDDGKTVAA MDMLVPRIGE LIGGSQREER
510 520 530 540 550
LEVLEARLDE LKLNKESYWW YLDLRRYGSV PHAGFGLGFE RLVQFVTGID
560
NIRDVIPFPR TPASAEF
Length:567
Mass (Da):63,698
Last modified:November 9, 2004 - v3
Checksum:iD5CAE4EBB7780264
GO

Sequence cautioni

The sequence CAB10511 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB78733 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti279N → Y in CAA10904 (PubMed:9655910).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z97343 Genomic DNA. Translation: CAB10511.1. Sequence problems.
AL161546 Genomic DNA. Translation: CAB78733.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE83874.1.
AY078967 mRNA. Translation: AAL84964.1.
AJ222644 mRNA. Translation: CAA10904.1.
RefSeqiNP_193462.1. NM_117835.5.
UniGeneiAt.123.
At.26556.

Genome annotation databases

EnsemblPlantsiAT4G17300.1; AT4G17300.1; AT4G17300.
GeneIDi827443.
GrameneiAT4G17300.1; AT4G17300.1; AT4G17300.
KEGGiath:AT4G17300.

Similar proteinsi

Entry informationi

Entry nameiSYNO_ARATH
AccessioniPrimary (citable) accession number: O48593
Secondary accession number(s): O23573, Q564D6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: November 9, 2004
Last modified: September 27, 2017
This is version 131 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families