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O48540

- MAN1_SOLLC

UniProt

O48540 - MAN1_SOLLC

Protein

Mannan endo-1,4-beta-mannosidase 1

Gene

MAN1

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 2 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei199 – 1991Proton donorBy similarity
    Active sitei316 – 3161NucleophileBy similarity

    GO - Molecular functioni

    1. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH5. Glycoside Hydrolase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannan endo-1,4-beta-mannosidase 1 (EC:3.2.1.78)
    Alternative name(s):
    Beta-mannanase 1
    Endo-beta-1,4-mannanase 1
    LeMAN1
    Gene namesi
    Name:MAN1
    OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
    Taxonomic identifieri4081 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
    ProteomesiUP000004994: Unplaced

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 23231 PublicationAdd
    BLAST
    Chaini24 – 397374Mannan endo-1,4-beta-mannosidase 1PRO_0000277491Add
    BLAST

    Expressioni

    Developmental stagei

    Expressed in lateral endosperm after radicle emergence (72 hours germinated seeds). Expressed in fruit pericarp during the early stages of ripening.2 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliO48540.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    OMAiEPAIMAW.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001547. Glyco_hydro_5.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00150. Cellulase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O48540-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYARRSCIC GLFLLFLALV CEANSGFIGV KDSHFELNGS PFLFNGFNSY    50
    WLMHVAADPT ERYKVTEVLK DASVAGLSVC RTWAFSDGGD RALQISPGIY 100
    DERVFQGLDF VIAEAKKYGI RLILSFVNQW NDFGGKAQYV WWARNAGAQI 150
    SNDDEFYTHP MLKKYLKNHI EKVVTRLNSI TKVAYKDDPT IMAWELMNEP 200
    RDQADYSGKT VNGWVQEMAS FVKSLDNKHL LEVGMEGFYG DSIPERKSVN 250
    PGYQVGTDFI SNHLINEIDF ATIHAYTDQW VSGQSDDAQL VWMEKWITSH 300
    WEDARNILKK PLVLAEFGKS SRGQGSRDIF MSSVYRNVYN LAKEGGTMAG 350
    SLVWQLMAHG MENYDDGYCI VLGQTPSTTQ IISDQAHVMT ALARSLN 397
    Length:397
    Mass (Da):44,804
    Last modified:November 23, 2004 - v2
    Checksum:i48FD322D2B5ED37F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF017144 mRNA. Translation: AAB87859.2.
    PIRiT04323.
    RefSeqiNP_001234575.1. NM_001247646.1.
    UniGeneiLes.234.

    Genome annotation databases

    EnsemblPlantsiSolyc00g231680.1.1; Solyc00g231680.1.1; Solyc00g231680.1.
    GeneIDi544199.
    KEGGisly:544199.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF017144 mRNA. Translation: AAB87859.2 .
    PIRi T04323.
    RefSeqi NP_001234575.1. NM_001247646.1.
    UniGenei Les.234.

    3D structure databases

    ProteinModelPortali O48540.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH5. Glycoside Hydrolase Family 5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi Solyc00g231680.1.1 ; Solyc00g231680.1.1 ; Solyc00g231680.1 .
    GeneIDi 544199.
    KEGGi sly:544199.

    Phylogenomic databases

    OMAi EPAIMAW.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001547. Glyco_hydro_5.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00150. Cellulase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a cDNA encoding a (1-->4)-beta-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum)."
      Bewley J.D., Burton R.A., Morohashi Y., Fincher G.B.
      Planta 203:454-459(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-88.
      Tissue: Seed.
    2. "Endo-beta-mannanase activity increases in the skin and outer pericarp of tomato fruits during ripening."
      Bewley J.D., Banik M., Bourgault R., Feurtado J.A., Toorop P., Hilhorst H.W.M.
      J. Exp. Bot. 51:529-538(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    3. "A germination-specific endo-beta-mannanase gene is expressed exclusively in the micropylar endosperm cap of tomato seeds."
      Nonogaki H., Gee O.H., Bradford K.J.
      Plant Physiol. 123:1235-1246(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiMAN1_SOLLC
    AccessioniPrimary (citable) accession number: O48540
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 6, 2007
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 62 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3