O48540 (MAN1_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannan endo-1,4-beta-mannosidase 1
EC=3.2.1.78

Alternative name(s):
Beta-mannanase 1
Endo-beta-1,4-mannanase 1
LeMAN1

Gene names

Name:

MAN1

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Subcellular location	Secreted Potential.
Developmental stage	Expressed in lateral endosperm after radicle emergence (72 hours germinated seeds). Expressed in fruit pericarp during the early stages of ripening. Ref.2 Ref.3
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	mannan endo-1,4-beta-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.1
	Chain	24 – 397	374	Mannan endo-1,4-beta-mannosidase 1		PRO_0000277491
Sites
	Active site	199	1	Proton donor By similarity
	Active site	316	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	O48540 [UniParc]. Last modified November 23, 2004. Version 2. Checksum: 48FD322D2B5ED37F	FASTA	397	44,804
	10 20 30 40 50 60 MSYARRSCIC GLFLLFLALV CEANSGFIGV KDSHFELNGS PFLFNGFNSY WLMHVAADPT 70 80 90 100 110 120 ERYKVTEVLK DASVAGLSVC RTWAFSDGGD RALQISPGIY DERVFQGLDF VIAEAKKYGI 130 140 150 160 170 180 RLILSFVNQW NDFGGKAQYV WWARNAGAQI SNDDEFYTHP MLKKYLKNHI EKVVTRLNSI 190 200 210 220 230 240 TKVAYKDDPT IMAWELMNEP RDQADYSGKT VNGWVQEMAS FVKSLDNKHL LEVGMEGFYG 250 260 270 280 290 300 DSIPERKSVN PGYQVGTDFI SNHLINEIDF ATIHAYTDQW VSGQSDDAQL VWMEKWITSH 310 320 330 340 350 360 WEDARNILKK PLVLAEFGKS SRGQGSRDIF MSSVYRNVYN LAKEGGTMAG SLVWQLMAHG 370 380 390 MENYDDGYCI VLGQTPSTTQ IISDQAHVMT ALARSLN

References

[1]	"Molecular cloning of a cDNA encoding a (1-->4)-beta-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum)." Bewley J.D., Burton R.A., Morohashi Y., Fincher G.B. Planta 203:454-459(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-88. Tissue: Seed.
[2]	"Endo-beta-mannanase activity increases in the skin and outer pericarp of tomato fruits during ripening." Bewley J.D., Banik M., Bourgault R., Feurtado J.A., Toorop P., Hilhorst H.W.M. J. Exp. Bot. 51:529-538(2000) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
[3]	"A germination-specific endo-beta-mannanase gene is expressed exclusively in the micropylar endosperm cap of tomato seeds." Nonogaki H., Gee O.H., Bradford K.J. Plant Physiol. 123:1235-1246(2000) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF017144 mRNA. Translation: AAB87859.2.
PIR	T04323.
RefSeq	NP_001234575.1. NM_001247646.1.
UniGene	Les.234.
3D structure databases
ProteinModelPortal	O48540.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	Solyc00g231680.1.1; Solyc00g231680.1.1; Solyc00g231680.1.
GeneID	544199.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAN1_SOLLC

Accession

Primary (citable) accession number: O48540

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program