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Protein

Mannan endo-1,4-beta-mannosidase 1

Gene

MAN1

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei199 – 1991Proton donorBy similarity
Active sitei316 – 3161NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase 1 (EC:3.2.1.78)
Alternative name(s):
Beta-mannanase 1
Endo-beta-1,4-mannanase 1
LeMAN1
Gene namesi
Name:MAN1
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 397374Mannan endo-1,4-beta-mannosidase 1PRO_0000277491Add
BLAST

Expressioni

Developmental stagei

Expressed in lateral endosperm after radicle emergence (72 hours germinated seeds). Expressed in fruit pericarp during the early stages of ripening.2 Publications

Structurei

3D structure databases

ProteinModelPortaliO48540.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiO48540.
OMAiIISDQAH.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O48540-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYARRSCIC GLFLLFLALV CEANSGFIGV KDSHFELNGS PFLFNGFNSY
60 70 80 90 100
WLMHVAADPT ERYKVTEVLK DASVAGLSVC RTWAFSDGGD RALQISPGIY
110 120 130 140 150
DERVFQGLDF VIAEAKKYGI RLILSFVNQW NDFGGKAQYV WWARNAGAQI
160 170 180 190 200
SNDDEFYTHP MLKKYLKNHI EKVVTRLNSI TKVAYKDDPT IMAWELMNEP
210 220 230 240 250
RDQADYSGKT VNGWVQEMAS FVKSLDNKHL LEVGMEGFYG DSIPERKSVN
260 270 280 290 300
PGYQVGTDFI SNHLINEIDF ATIHAYTDQW VSGQSDDAQL VWMEKWITSH
310 320 330 340 350
WEDARNILKK PLVLAEFGKS SRGQGSRDIF MSSVYRNVYN LAKEGGTMAG
360 370 380 390
SLVWQLMAHG MENYDDGYCI VLGQTPSTTQ IISDQAHVMT ALARSLN
Length:397
Mass (Da):44,804
Last modified:November 23, 2004 - v2
Checksum:i48FD322D2B5ED37F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017144 mRNA. Translation: AAB87859.2.
PIRiT04323.
RefSeqiNP_001234575.1. NM_001247646.1.
UniGeneiLes.234.

Genome annotation databases

EnsemblPlantsiSolyc00g231680.1.1; Solyc00g231680.1.1; Solyc00g231680.1.
GeneIDi544199.
KEGGisly:544199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017144 mRNA. Translation: AAB87859.2.
PIRiT04323.
RefSeqiNP_001234575.1. NM_001247646.1.
UniGeneiLes.234.

3D structure databases

ProteinModelPortaliO48540.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiSolyc00g231680.1.1; Solyc00g231680.1.1; Solyc00g231680.1.
GeneIDi544199.
KEGGisly:544199.

Phylogenomic databases

InParanoidiO48540.
OMAiIISDQAH.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of a cDNA encoding a (1-->4)-beta-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum)."
    Bewley J.D., Burton R.A., Morohashi Y., Fincher G.B.
    Planta 203:454-459(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-88.
    Tissue: Seed.
  2. "Endo-beta-mannanase activity increases in the skin and outer pericarp of tomato fruits during ripening."
    Bewley J.D., Banik M., Bourgault R., Feurtado J.A., Toorop P., Hilhorst H.W.M.
    J. Exp. Bot. 51:529-538(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  3. "A germination-specific endo-beta-mannanase gene is expressed exclusively in the micropylar endosperm cap of tomato seeds."
    Nonogaki H., Gee O.H., Bradford K.J.
    Plant Physiol. 123:1235-1246(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiMAN1_SOLLC
AccessioniPrimary (citable) accession number: O48540
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: November 23, 2004
Last modified: July 22, 2015
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.