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O48540 (MAN1_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannan endo-1,4-beta-mannosidase 1

EC=3.2.1.78
Alternative name(s):
Beta-mannanase 1
Endo-beta-1,4-mannanase 1
LeMAN1
Gene names
Name:MAN1
OrganismSolanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier4081 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon

Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Subcellular location

Secreted Potential.

Developmental stage

Expressed in lateral endosperm after radicle emergence (72 hours germinated seeds). Expressed in fruit pericarp during the early stages of ripening. Ref.2 Ref.3

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmannan endo-1,4-beta-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.1
Chain24 – 397374Mannan endo-1,4-beta-mannosidase 1
PRO_0000277491

Sites

Active site1991Proton donor By similarity
Active site3161Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
O48540 [UniParc].

Last modified November 23, 2004. Version 2.
Checksum: 48FD322D2B5ED37F

FASTA39744,804
        10         20         30         40         50         60 
MSYARRSCIC GLFLLFLALV CEANSGFIGV KDSHFELNGS PFLFNGFNSY WLMHVAADPT 

        70         80         90        100        110        120 
ERYKVTEVLK DASVAGLSVC RTWAFSDGGD RALQISPGIY DERVFQGLDF VIAEAKKYGI 

       130        140        150        160        170        180 
RLILSFVNQW NDFGGKAQYV WWARNAGAQI SNDDEFYTHP MLKKYLKNHI EKVVTRLNSI 

       190        200        210        220        230        240 
TKVAYKDDPT IMAWELMNEP RDQADYSGKT VNGWVQEMAS FVKSLDNKHL LEVGMEGFYG 

       250        260        270        280        290        300 
DSIPERKSVN PGYQVGTDFI SNHLINEIDF ATIHAYTDQW VSGQSDDAQL VWMEKWITSH 

       310        320        330        340        350        360 
WEDARNILKK PLVLAEFGKS SRGQGSRDIF MSSVYRNVYN LAKEGGTMAG SLVWQLMAHG 

       370        380        390 
MENYDDGYCI VLGQTPSTTQ IISDQAHVMT ALARSLN 

« Hide

References

[1]"Molecular cloning of a cDNA encoding a (1-->4)-beta-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum)."
Bewley J.D., Burton R.A., Morohashi Y., Fincher G.B.
Planta 203:454-459(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-88.
Tissue: Seed.
[2]"Endo-beta-mannanase activity increases in the skin and outer pericarp of tomato fruits during ripening."
Bewley J.D., Banik M., Bourgault R., Feurtado J.A., Toorop P., Hilhorst H.W.M.
J. Exp. Bot. 51:529-538(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[3]"A germination-specific endo-beta-mannanase gene is expressed exclusively in the micropylar endosperm cap of tomato seeds."
Nonogaki H., Gee O.H., Bradford K.J.
Plant Physiol. 123:1235-1246(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF017144 mRNA. Translation: AAB87859.2.
PIRT04323.
RefSeqNP_001234575.1. NM_001247646.1.
UniGeneLes.234.

3D structure databases

ProteinModelPortalO48540.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsSolyc00g231680.1.1; Solyc00g231680.1.1; Solyc00g231680.1.
GeneID544199.
KEGGsly:544199.

Phylogenomic databases

OMAEPAIMAW.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMAN1_SOLLC
AccessionPrimary (citable) accession number: O48540
Entry history
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 61 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries