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O48540

- MAN1_SOLLC

UniProt

O48540 - MAN1_SOLLC

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Protein

Mannan endo-1,4-beta-mannosidase 1

Gene

MAN1

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei199 – 1991Proton donorBy similarity
Active sitei316 – 3161NucleophileBy similarity

GO - Molecular functioni

  1. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase 1 (EC:3.2.1.78)
Alternative name(s):
Beta-mannanase 1
Endo-beta-1,4-mannanase 1
LeMAN1
Gene namesi
Name:MAN1
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994: Unplaced

Subcellular locationi

Secreted Curated

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23231 PublicationAdd
BLAST
Chaini24 – 397374Mannan endo-1,4-beta-mannosidase 1PRO_0000277491Add
BLAST

Expressioni

Developmental stagei

Expressed in lateral endosperm after radicle emergence (72 hours germinated seeds). Expressed in fruit pericarp during the early stages of ripening.2 Publications

Structurei

3D structure databases

ProteinModelPortaliO48540.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiO48540.
OMAiEPAIMAW.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O48540-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSYARRSCIC GLFLLFLALV CEANSGFIGV KDSHFELNGS PFLFNGFNSY
60 70 80 90 100
WLMHVAADPT ERYKVTEVLK DASVAGLSVC RTWAFSDGGD RALQISPGIY
110 120 130 140 150
DERVFQGLDF VIAEAKKYGI RLILSFVNQW NDFGGKAQYV WWARNAGAQI
160 170 180 190 200
SNDDEFYTHP MLKKYLKNHI EKVVTRLNSI TKVAYKDDPT IMAWELMNEP
210 220 230 240 250
RDQADYSGKT VNGWVQEMAS FVKSLDNKHL LEVGMEGFYG DSIPERKSVN
260 270 280 290 300
PGYQVGTDFI SNHLINEIDF ATIHAYTDQW VSGQSDDAQL VWMEKWITSH
310 320 330 340 350
WEDARNILKK PLVLAEFGKS SRGQGSRDIF MSSVYRNVYN LAKEGGTMAG
360 370 380 390
SLVWQLMAHG MENYDDGYCI VLGQTPSTTQ IISDQAHVMT ALARSLN
Length:397
Mass (Da):44,804
Last modified:November 23, 2004 - v2
Checksum:i48FD322D2B5ED37F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF017144 mRNA. Translation: AAB87859.2.
PIRiT04323.
RefSeqiNP_001234575.1. NM_001247646.1.
UniGeneiLes.234.

Genome annotation databases

EnsemblPlantsiSolyc00g231680.1.1; Solyc00g231680.1.1; Solyc00g231680.1.
GeneIDi544199.
KEGGisly:544199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF017144 mRNA. Translation: AAB87859.2 .
PIRi T04323.
RefSeqi NP_001234575.1. NM_001247646.1.
UniGenei Les.234.

3D structure databases

ProteinModelPortali O48540.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi Solyc00g231680.1.1 ; Solyc00g231680.1.1 ; Solyc00g231680.1 .
GeneIDi 544199.
KEGGi sly:544199.

Phylogenomic databases

InParanoidi O48540.
OMAi EPAIMAW.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001547. Glyco_hydro_5.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00150. Cellulase. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of a cDNA encoding a (1-->4)-beta-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum)."
    Bewley J.D., Burton R.A., Morohashi Y., Fincher G.B.
    Planta 203:454-459(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-88.
    Tissue: Seed.
  2. "Endo-beta-mannanase activity increases in the skin and outer pericarp of tomato fruits during ripening."
    Bewley J.D., Banik M., Bourgault R., Feurtado J.A., Toorop P., Hilhorst H.W.M.
    J. Exp. Bot. 51:529-538(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  3. "A germination-specific endo-beta-mannanase gene is expressed exclusively in the micropylar endosperm cap of tomato seeds."
    Nonogaki H., Gee O.H., Bradford K.J.
    Plant Physiol. 123:1235-1246(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiMAN1_SOLLC
AccessioniPrimary (citable) accession number: O48540
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: November 23, 2004
Last modified: October 29, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3