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Protein

Respiratory burst oxidase homolog protein F

Gene

RBOHF

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent NADPH oxidase that generates superoxide. Generates reactive oxygen species (ROS) during incompatible interactions with pathogens and is important in the regulation of the hypersensitive response (HR). Involved in abscisic acid-induced stomatal closing and in UV-B and abscisic acid ROS-dependent signaling.5 Publications

Enzyme regulationi

Inhibited by diphenylene iodonium (DPI).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi277CalciumBy similarity1
Metal bindingi279CalciumBy similarity1
Metal bindingi281CalciumBy similarity1
Metal bindingi283Calcium; via carbonyl oxygenBy similarity1
Metal bindingi288CalciumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi277 – 28811 PublicationAdd BLAST12
Calcium bindingi321 – 33221 PublicationAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • NAD(P)H oxidase activity Source: TAIR
  • peroxidase activity Source: UniProtKB-KW

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • carbohydrate homeostasis Source: TAIR
  • defense response by callose deposition Source: TAIR
  • ethylene-activated signaling pathway Source: TAIR
  • hydrogen peroxide biosynthetic process Source: TAIR
  • negative regulation of programmed cell death Source: TAIR
  • osmosensory signaling pathway Source: TAIR
  • regulation of stomatal movement Source: TAIR
  • respiratory burst involved in defense response Source: TAIR
  • response to ethylene Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Ligandi

Calcium, FAD, Flavoprotein, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT1G64060-MONOMER.
ReactomeiR-ATH-209968. Thyroxine biosynthesis.
R-ATH-3299685. Detoxification of Reactive Oxygen Species.
R-ATH-6798695. Neutrophil degranulation.

Protein family/group databases

TCDBi5.B.1.1.9. the phagocyte (gp91(phox)) nadph oxidase family.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory burst oxidase homolog protein F (EC:1.11.1.-, EC:1.6.3.-)
Alternative name(s):
Cytochrome b245 beta chain homolog RbohAp108
NADPH oxidase RBOHF
Short name:
AtRBOHF
Gene namesi
Name:RBOHF
Synonyms:RBOHAP108
Ordered Locus Names:At1g64060
ORF Names:F22C12.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G64060.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 387CytoplasmicSequence analysisAdd BLAST386
Transmembranei388 – 408Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini409 – 475ExtracellularSequence analysisAdd BLAST67
Transmembranei476 – 492Helical; Name=2By similarityAdd BLAST17
Topological domaini493 – 527CytoplasmicSequence analysisAdd BLAST35
Transmembranei528 – 548Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini549 – 570ExtracellularSequence analysisAdd BLAST22
Transmembranei571 – 591Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini592 – 599CytoplasmicSequence analysis8
Transmembranei600 – 617Helical; Name=5Sequence analysisAdd BLAST18
Topological domaini618 – 744ExtracellularSequence analysisAdd BLAST127
Transmembranei745 – 765Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini766 – 944CytoplasmicSequence analysisAdd BLAST179

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00003137582 – 944Respiratory burst oxidase homolog protein FAdd BLAST943

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei354PhosphoserineBy similarity1
Modified residuei358PhosphoserineBy similarity1

Post-translational modificationi

Not glycosylated. Phosphorylated by CIPK26.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO48538.
PRIDEiO48538.

PTM databases

iPTMnetiO48538.

Expressioni

Tissue specificityi

Expressed in roots, stems, seedlings, inflorescences, leaves and guard cells.3 Publications

Inductioni

Up-regulated by abscisic acid.1 Publication

Gene expression databases

GenevisibleiO48538. AT.

Interactioni

Subunit structurei

Monomer and homodimer (By similarity). Interacts (via N-terminus) with CIPK26 (PubMed:23335733). Interacts (via N-terminus) with SRC2 (PubMed:23872431).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SRK2EQ940H64EBI-7197253,EBI-782514

Protein-protein interaction databases

BioGridi27931. 3 interactors.
IntActiO48538. 1 interactor.
MINTiMINT-7260067.
STRINGi3702.AT1G64060.1.

Structurei

3D structure databases

ProteinModelPortaliO48538.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini264 – 299EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini308 – 343EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini426 – 583Ferric oxidoreductaseAdd BLAST158
Domaini622 – 742FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST121

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni207 – 215EF-hand-like 1By similarity9
Regioni241 – 252EF-hand-like 2By similarityAdd BLAST12

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili102 – 126Sequence analysisAdd BLAST25
Coiled coili157 – 184Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi239 – 242Poly-Arg4

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000216670.
InParanoidiO48538.
KOiK13447.
OMAiFYQYKQK.
OrthoDBiEOG093601IF.
PhylomeDBiO48538.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O48538-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPFSKNDRR RWSFDSVSAG KTAVGSASTS PGTEYSINGD QEFVEVTIDL
60 70 80 90 100
QDDDTIVLRS VEPATAINVI GDISDDNTGI MTPVSISRSP TMKRTSSNRF
110 120 130 140 150
RQFSQELKAE AVAKAKQLSQ ELKRFSWSRS FSGNLTTTST AANQSGGAGG
160 170 180 190 200
GLVNSALEAR ALRKQRAQLD RTRSSAQRAL RGLRFISNKQ KNVDGWNDVQ
210 220 230 240 250
SNFEKFEKNG YIYRSDFAQC IGMKDSKEFA LELFDALSRR RRLKVEKINH
260 270 280 290 300
DELYEYWSQI NDESFDSRLQ IFFDIVDKNE DGRITEEEVK EIIMLSASAN
310 320 330 340 350
KLSRLKEQAE EYAALIMEEL DPERLGYIEL WQLETLLLQK DTYLNYSQAL
360 370 380 390 400
SYTSQALSQN LQGLRGKSRI HRMSSDFVYI MQENWKRIWV LSLWIMIMIG
410 420 430 440 450
LFLWKFFQYK QKDAFHVMGY CLLTAKGAAE TLKFNMALIL FPVCRNTITW
460 470 480 490 500
LRSTRLSYFV PFDDNINFHK TIAGAIVVAV ILHIGDHLAC DFPRIVRATE
510 520 530 540 550
YDYNRYLFHY FQTKQPTYFD LVKGPEGITG ILMVILMIIS FTLATRWFRR
560 570 580 590 600
NLVKLPKPFD RLTGFNAFWY SHHLFVIVYI LLILHGIFLY FAKPWYVRTT
610 620 630 640 650
WMYLAVPVLL YGGERTLRYF RSGSYSVRLL KVAIYPGNVL TLQMSKPTQF
660 670 680 690 700
RYKSGQYMFV QCPAVSPFEW HPFSITSAPE DDYISIHIRQ LGDWTQELKR
710 720 730 740 750
VFSEVCEPPV GGKSGLLRAD ETTKKSLPKL LIDGPYGAPA QDYRKYDVLL
760 770 780 790 800
LVGLGIGATP FISILKDLLN NIVKMEEHAD SISDFSRSSE YSTGSNGDTP
810 820 830 840 850
RRKRILKTTN AYFYWVTREQ GSFDWFKGVM NEVAELDQRG VIEMHNYLTS
860 870 880 890 900
VYEEGDARSA LITMVQALNH AKNGVDIVSG TRVRTHFARP NWKKVLTKLS
910 920 930 940
SKHCNARIGV FYCGVPVLGK ELSKLCNTFN QKGSTKFEFH KEHF
Length:944
Mass (Da):108,418
Last modified:June 1, 1998 - v1
Checksum:i1ABAEC316AE80F9B
GO

Sequence cautioni

The sequence AAF24574 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAF00352 differs from that shown. Intron retention.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti908I → T in BAA28953 (PubMed:9628030).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008111 mRNA. Translation: BAA28953.1.
AF015301 mRNA. Translation: AAB87789.1.
AC007764 Genomic DNA. Translation: AAF24574.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34186.1.
AK228418 mRNA. Translation: BAF00352.1. Sequence problems.
PIRiT03826.
RefSeqiNP_564821.1. NM_105079.3.
UniGeneiAt.235.

Genome annotation databases

EnsemblPlantsiAT1G64060.1; AT1G64060.1; AT1G64060.
GeneIDi842710.
GrameneiAT1G64060.1; AT1G64060.1; AT1G64060.
KEGGiath:AT1G64060.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008111 mRNA. Translation: BAA28953.1.
AF015301 mRNA. Translation: AAB87789.1.
AC007764 Genomic DNA. Translation: AAF24574.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE34186.1.
AK228418 mRNA. Translation: BAF00352.1. Sequence problems.
PIRiT03826.
RefSeqiNP_564821.1. NM_105079.3.
UniGeneiAt.235.

3D structure databases

ProteinModelPortaliO48538.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27931. 3 interactors.
IntActiO48538. 1 interactor.
MINTiMINT-7260067.
STRINGi3702.AT1G64060.1.

Protein family/group databases

TCDBi5.B.1.1.9. the phagocyte (gp91(phox)) nadph oxidase family.

PTM databases

iPTMnetiO48538.

Proteomic databases

PaxDbiO48538.
PRIDEiO48538.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G64060.1; AT1G64060.1; AT1G64060.
GeneIDi842710.
GrameneiAT1G64060.1; AT1G64060.1; AT1G64060.
KEGGiath:AT1G64060.

Organism-specific databases

TAIRiAT1G64060.

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000216670.
InParanoidiO48538.
KOiK13447.
OMAiFYQYKQK.
OrthoDBiEOG093601IF.
PhylomeDBiO48538.

Enzyme and pathway databases

BioCyciARA:AT1G64060-MONOMER.
ReactomeiR-ATH-209968. Thyroxine biosynthesis.
R-ATH-3299685. Detoxification of Reactive Oxygen Species.
R-ATH-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiO48538.

Gene expression databases

GenevisibleiO48538. AT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBOHF_ARATH
AccessioniPrimary (citable) accession number: O48538
Secondary accession number(s): O80342, Q0WR97, Q9SH56
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was originally called RBOHA.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.