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O48528 (OP163_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer envelope pore protein 16-3, chloroplastic/mitochondrial
Alternative name(s):
Chloroplastic outer envelope pore protein of 16 kDa 3
Short name=AtOEP16-3
Short name=OEP16-3
Mitochondrial complex I subunit B14.7
Gene names
Name:OEP163
Synonyms:B14.7
Ordered Locus Names:At2g42210
ORF Names:T24P15.12
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length159 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Voltage-dependent high-conductance channel with a slightly cation-selectivity; selective for amino acids but excludes triosephosphates or uncharged sugars. Non-essential amino acid-selective channel protein and translocation pore for NADPH:protochlorophyllide oxidoreductase A (PORA) and possibly PORB By similarity.

Subunit structure

Homodimer and oligomers in membrane By similarity. Part of both the NADH-ubiquinone oxidoreductase complex I and of the TIM17:23 complex. Interacts with TIM23-2. Ref.10 Ref.12 Ref.14

Subcellular location

Plastidchloroplast outer membrane; Multi-pass membrane protein. Mitochondrion outer membrane; Multi-pass membrane protein. Mitochondrion inner membrane; Multi-pass membrane protein Potential Ref.1 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.14.

Disruption phenotype

Lethal when homozygous. Ref.14

Sequence similarities

Belongs to the Tim17/Tim22/Tim23 family. Plastid outer envelope porin OEP16 (TC 1.B.30) subfamily. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O48528-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O48528-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MFNLCALGRTVEEIM
Note: Derived from EST data. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 159159Outer envelope pore protein 16-3, chloroplastic/mitochondrial
PRO_0000415698

Regions

Transmembrane24 – 4017Helical; Potential
Transmembrane62 – 7918Helical; Potential
Transmembrane92 – 10918Helical; Potential
Region1 – 6565Contains beta strands By similarity

Natural variations

Alternative sequence11M → MFNLCALGRTVEEIM in isoform 2.
VSP_042321

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 3102BC2ACFA389E7

FASTA15916,999
        10         20         30         40         50         60 
MDPAEMRYLE EEDGPLMKTI KGSITGFGAG TIYGTILATW KDVPRVERNV ALPGLIRTLK 

        70         80         90        100        110        120 
MMGTHGLTFA AIGGVYIGVE QLVQNFRSKR DFYNGAIGGF VAGASVLGYR ARSIPTAIAA 

       130        140        150 
GATLAVTSAL IDSGGQTTRV DNGREYYPYT VEKRAEADS 

« Hide

Isoform 2 [UniParc].

Checksum: A500096BBDC6C4D2
Show »

FASTA17318,577

References

« Hide 'large scale' references
[1]"Characterization of the preprotein and amino acid transporter gene family in Arabidopsis."
Murcha M.W., Elhafez D., Lister R., Tonti-Filippini J., Baumgartner M., Philippar K., Carrie C., Mokranjac D., Soll J., Whelan J.
Plant Physiol. 143:199-212(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[6]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Landsberg erecta.
[7]"Mapping the Arabidopsis organelle proteome."
Dunkley T.P.J., Hester S., Shadforth I.P., Runions J., Weimar T., Hanton S.L., Griffin J.L., Bessant C., Brandizzi F., Hawes C., Watson R.B., Dupree P., Lilley K.S.
Proc. Natl. Acad. Sci. U.S.A. 103:6518-6523(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Mitochondrial acyl carrier proteins in Arabidopsis thaliana are predominantly soluble matrix proteins and none can be confirmed as subunits of respiratory Complex I."
Meyer E.H., Heazlewood J.L., Millar A.H.
Plant Mol. Biol. 64:319-327(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, GENE FAMILY.
[9]"Chloroplast biogenesis: the use of mutants to study the etioplast-chloroplast transition."
Philippar K., Geis T., Ilkavets I., Oster U., Schwenkert S., Meurer J., Soll J.
Proc. Natl. Acad. Sci. U.S.A. 104:678-683(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[10]"Resolving and identifying protein components of plant mitochondrial respiratory complexes using three dimensions of gel electrophoresis."
Meyer E.H., Taylor N.L., Millar A.H.
J. Proteome Res. 7:786-794(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, SUBCELLULAR LOCATION.
[11]"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[12]"Defining the protein complex proteome of plant mitochondria."
Klodmann J., Senkler M., Rode C., Braun H.-P.
Plant Physiol. 157:587-598(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, SUBCELLULAR LOCATION.
[13]"Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Dual location of the mitochondrial preprotein transporters B14.7 and Tim23-2 in complex I and the TIM17:23 complex in Arabidopsis links mitochondrial activity and biogenesis."
Wang Y., Carrie C., Giraud E., Elhafez D., Narsai R., Duncan O., Whelan J., Murcha M.W.
Plant Cell 24:2675-2695(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TIM23-2, SUBCELLULAR LOCATION, SUBUNIT, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ386643 mRNA. Translation: ABD48955.1.
AC002561 Genomic DNA. Translation: AAB88646.1.
CP002685 Genomic DNA. Translation: AEC10086.1.
CP002685 Genomic DNA. Translation: AEC10087.1.
CP002685 Genomic DNA. Translation: AEC10088.1.
CP002685 Genomic DNA. Translation: AEC10089.1.
AY086879 mRNA. Translation: AAM63925.1.
BT025617 mRNA. Translation: ABF59035.1.
PIRT00930.
RefSeqNP_001031527.1. NM_001036450.1.
NP_001031528.1. NM_001036451.2.
NP_001031529.1. NM_001036452.1.
NP_565968.1. NM_129783.3.
UniGeneAt.12442.
At.67859.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO48528. 1 interaction.
STRING3702.AT2G42210.2-P.

Proteomic databases

PaxDbO48528.
PRIDEO48528.

Protocols and materials databases

DNASU818821.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G42210.1; AT2G42210.1; AT2G42210. [O48528-1]
AT2G42210.3; AT2G42210.3; AT2G42210. [O48528-1]
AT2G42210.4; AT2G42210.4; AT2G42210. [O48528-1]
GeneID818821.
KEGGath:AT2G42210.

Organism-specific databases

TAIRAT2G42210.

Phylogenomic databases

eggNOGNOG273361.
HOGENOMHOG000239422.
OMAVDNGKEY.
ProtClustDBCLSN2688882.

Gene expression databases

GenevestigatorO48528.

Family and domain databases

InterProIPR003397. Tim17/Tim22/Tim23/PMP24.
[Graphical view]
PfamPF02466. Tim17. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameOP163_ARATH
AccessionPrimary (citable) accession number: O48528
Secondary accession number(s): F4IMZ5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names