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Protein

Outer envelope pore protein 16-3, chloroplastic/mitochondrial

Gene

OEP163

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-dependent high-conductance channel with a slightly cation-selectivity; selective for amino acids but excludes triosephosphates or uncharged sugars. Non-essential amino acid-selective channel protein and translocation pore for NADPH:protochlorophyllide oxidoreductase A (PORA) and possibly PORB (By similarity).By similarity

GO - Molecular functioni

  1. porin activity Source: UniProtKB-KW
  2. protein homodimerization activity Source: UniProtKB

GO - Biological processi

  1. ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Outer envelope pore protein 16-3, chloroplastic/mitochondrial
Alternative name(s):
Chloroplastic outer envelope pore protein of 16 kDa 3
Short name:
AtOEP16-3
Short name:
OEP16-3
Mitochondrial complex I subunit B14.7
Gene namesi
Name:OEP163
Synonyms:B14.7
Ordered Locus Names:At2g42210
ORF Names:T24P15.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G42210.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei24 – 4017HelicalSequence AnalysisAdd
BLAST
Transmembranei62 – 7918HelicalSequence AnalysisAdd
BLAST
Transmembranei92 – 10918HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast outer membrane Source: UniProtKB-SubCell
  3. membrane Source: TAIR
  4. mitochondrial inner membrane Source: UniProtKB-SubCell
  5. mitochondrial outer membrane Source: UniProtKB-SubCell
  6. mitochondrial respiratory chain complex I Source: TAIR
  7. mitochondrion Source: TAIR
  8. plastid outer membrane Source: TAIR
  9. pore complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Mitochondrion, Mitochondrion inner membrane, Mitochondrion outer membrane, Plastid, Plastid outer membrane

Pathology & Biotechi

Disruption phenotypei

Lethal when homozygous.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 159159Outer envelope pore protein 16-3, chloroplastic/mitochondrialPRO_0000415698Add
BLAST

Proteomic databases

PaxDbiO48528.
PRIDEiO48528.

Expressioni

Gene expression databases

GenevestigatoriO48528.

Interactioni

Subunit structurei

Homodimer and oligomers in membrane (By similarity). Part of both the NADH-ubiquinone oxidoreductase complex I and of the TIM17:23 complex. Interacts with TIM23-2.By similarity3 Publications

Protein-protein interaction databases

IntActiO48528. 1 interaction.
STRINGi3702.AT2G42210.2-P.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 6565Contains beta strandsBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane beta strand, Transmembrane helix

Phylogenomic databases

eggNOGiNOG273361.
HOGENOMiHOG000239422.
InParanoidiO48528.
OMAiIRTLKMM.

Family and domain databases

InterProiIPR003397. Tim17/Tim22/Tim23/PMP24.
[Graphical view]
PfamiPF02466. Tim17. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O48528-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPAEMRYLE EEDGPLMKTI KGSITGFGAG TIYGTILATW KDVPRVERNV
60 70 80 90 100
ALPGLIRTLK MMGTHGLTFA AIGGVYIGVE QLVQNFRSKR DFYNGAIGGF
110 120 130 140 150
VAGASVLGYR ARSIPTAIAA GATLAVTSAL IDSGGQTTRV DNGREYYPYT

VEKRAEADS
Length:159
Mass (Da):16,999
Last modified:June 1, 1998 - v1
Checksum:i3102BC2ACFA389E7
GO
Isoform 2 (identifier: O48528-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MFNLCALGRTVEEIM

Note: Derived from EST data. No experimental confirmation available.

Show »
Length:173
Mass (Da):18,577
Checksum:iA500096BBDC6C4D2
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MFNLCALGRTVEEIM in isoform 2. CuratedVSP_042321

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ386643 mRNA. Translation: ABD48955.1.
AC002561 Genomic DNA. Translation: AAB88646.1.
CP002685 Genomic DNA. Translation: AEC10086.1.
CP002685 Genomic DNA. Translation: AEC10087.1.
CP002685 Genomic DNA. Translation: AEC10088.1.
CP002685 Genomic DNA. Translation: AEC10089.1.
AY086879 mRNA. Translation: AAM63925.1.
BT025617 mRNA. Translation: ABF59035.1.
PIRiT00930.
RefSeqiNP_001031527.1. NM_001036450.1. [O48528-2]
NP_001031528.1. NM_001036451.2. [O48528-1]
NP_001031529.1. NM_001036452.1. [O48528-1]
NP_565968.1. NM_129783.3. [O48528-1]
UniGeneiAt.12442.
At.67859.

Genome annotation databases

EnsemblPlantsiAT2G42210.1; AT2G42210.1; AT2G42210. [O48528-1]
AT2G42210.2; AT2G42210.2; AT2G42210. [O48528-2]
AT2G42210.3; AT2G42210.3; AT2G42210. [O48528-1]
AT2G42210.4; AT2G42210.4; AT2G42210. [O48528-1]
GeneIDi818821.
KEGGiath:AT2G42210.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ386643 mRNA. Translation: ABD48955.1.
AC002561 Genomic DNA. Translation: AAB88646.1.
CP002685 Genomic DNA. Translation: AEC10086.1.
CP002685 Genomic DNA. Translation: AEC10087.1.
CP002685 Genomic DNA. Translation: AEC10088.1.
CP002685 Genomic DNA. Translation: AEC10089.1.
AY086879 mRNA. Translation: AAM63925.1.
BT025617 mRNA. Translation: ABF59035.1.
PIRiT00930.
RefSeqiNP_001031527.1. NM_001036450.1. [O48528-2]
NP_001031528.1. NM_001036451.2. [O48528-1]
NP_001031529.1. NM_001036452.1. [O48528-1]
NP_565968.1. NM_129783.3. [O48528-1]
UniGeneiAt.12442.
At.67859.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO48528. 1 interaction.
STRINGi3702.AT2G42210.2-P.

Proteomic databases

PaxDbiO48528.
PRIDEiO48528.

Protocols and materials databases

DNASUi818821.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G42210.1; AT2G42210.1; AT2G42210. [O48528-1]
AT2G42210.2; AT2G42210.2; AT2G42210. [O48528-2]
AT2G42210.3; AT2G42210.3; AT2G42210. [O48528-1]
AT2G42210.4; AT2G42210.4; AT2G42210. [O48528-1]
GeneIDi818821.
KEGGiath:AT2G42210.

Organism-specific databases

TAIRiAT2G42210.

Phylogenomic databases

eggNOGiNOG273361.
HOGENOMiHOG000239422.
InParanoidiO48528.
OMAiIRTLKMM.

Gene expression databases

GenevestigatoriO48528.

Family and domain databases

InterProiIPR003397. Tim17/Tim22/Tim23/PMP24.
[Graphical view]
PfamiPF02466. Tim17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the preprotein and amino acid transporter gene family in Arabidopsis."
    Murcha M.W., Elhafez D., Lister R., Tonti-Filippini J., Baumgartner M., Philippar K., Carrie C., Mokranjac D., Soll J., Whelan J.
    Plant Physiol. 143:199-212(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. "Mitochondrial acyl carrier proteins in Arabidopsis thaliana are predominantly soluble matrix proteins and none can be confirmed as subunits of respiratory Complex I."
    Meyer E.H., Heazlewood J.L., Millar A.H.
    Plant Mol. Biol. 64:319-327(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, GENE FAMILY.
  9. "Chloroplast biogenesis: the use of mutants to study the etioplast-chloroplast transition."
    Philippar K., Geis T., Ilkavets I., Oster U., Schwenkert S., Meurer J., Soll J.
    Proc. Natl. Acad. Sci. U.S.A. 104:678-683(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  10. "Resolving and identifying protein components of plant mitochondrial respiratory complexes using three dimensions of gel electrophoresis."
    Meyer E.H., Taylor N.L., Millar A.H.
    J. Proteome Res. 7:786-794(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, SUBCELLULAR LOCATION.
  11. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  12. "Defining the protein complex proteome of plant mitochondria."
    Klodmann J., Senkler M., Rode C., Braun H.-P.
    Plant Physiol. 157:587-598(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, SUBCELLULAR LOCATION.
  13. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Dual location of the mitochondrial preprotein transporters B14.7 and Tim23-2 in complex I and the TIM17:23 complex in Arabidopsis links mitochondrial activity and biogenesis."
    Wang Y., Carrie C., Giraud E., Elhafez D., Narsai R., Duncan O., Whelan J., Murcha M.W.
    Plant Cell 24:2675-2695(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TIM23-2, SUBCELLULAR LOCATION, SUBUNIT, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiOP163_ARATH
AccessioniPrimary (citable) accession number: O48528
Secondary accession number(s): F4IMZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: June 1, 1998
Last modified: January 7, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.