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O48528

- OP163_ARATH

UniProt

O48528 - OP163_ARATH

Protein

Outer envelope pore protein 16-3, chloroplastic/mitochondrial

Gene

OEP163

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Voltage-dependent high-conductance channel with a slightly cation-selectivity; selective for amino acids but excludes triosephosphates or uncharged sugars. Non-essential amino acid-selective channel protein and translocation pore for NADPH:protochlorophyllide oxidoreductase A (PORA) and possibly PORB By similarity.By similarity

    GO - Molecular functioni

    1. porin activity Source: UniProtKB-KW
    2. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. ion transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Porin

    Keywords - Biological processi

    Ion transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer envelope pore protein 16-3, chloroplastic/mitochondrial
    Alternative name(s):
    Chloroplastic outer envelope pore protein of 16 kDa 3
    Short name:
    AtOEP16-3
    Short name:
    OEP16-3
    Mitochondrial complex I subunit B14.7
    Gene namesi
    Name:OEP163
    Synonyms:B14.7
    Ordered Locus Names:At2g42210
    ORF Names:T24P15.12
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G42210.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast outer membrane Source: UniProtKB-SubCell
    3. membrane Source: TAIR
    4. mitochondrial inner membrane Source: UniProtKB-SubCell
    5. mitochondrial outer membrane Source: UniProtKB-SubCell
    6. mitochondrial respiratory chain complex I Source: TAIR
    7. mitochondrion Source: TAIR
    8. plastid outer membrane Source: TAIR
    9. pore complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Chloroplast, Membrane, Mitochondrion, Mitochondrion inner membrane, Mitochondrion outer membrane, Plastid, Plastid outer membrane

    Pathology & Biotechi

    Disruption phenotypei

    Lethal when homozygous.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 159159Outer envelope pore protein 16-3, chloroplastic/mitochondrialPRO_0000415698Add
    BLAST

    Proteomic databases

    PaxDbiO48528.
    PRIDEiO48528.

    Expressioni

    Gene expression databases

    GenevestigatoriO48528.

    Interactioni

    Subunit structurei

    Homodimer and oligomers in membrane By similarity. Part of both the NADH-ubiquinone oxidoreductase complex I and of the TIM17:23 complex. Interacts with TIM23-2.By similarity3 Publications

    Protein-protein interaction databases

    IntActiO48528. 1 interaction.
    STRINGi3702.AT2G42210.2-P.

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei24 – 4017HelicalSequence AnalysisAdd
    BLAST
    Transmembranei62 – 7918HelicalSequence AnalysisAdd
    BLAST
    Transmembranei92 – 10918HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 6565Contains beta strandsBy similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane beta strand, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG273361.
    HOGENOMiHOG000239422.
    OMAiLYIGVEQ.

    Family and domain databases

    InterProiIPR003397. Tim17/Tim22/Tim23/PMP24.
    [Graphical view]
    PfamiPF02466. Tim17. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O48528-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDPAEMRYLE EEDGPLMKTI KGSITGFGAG TIYGTILATW KDVPRVERNV    50
    ALPGLIRTLK MMGTHGLTFA AIGGVYIGVE QLVQNFRSKR DFYNGAIGGF 100
    VAGASVLGYR ARSIPTAIAA GATLAVTSAL IDSGGQTTRV DNGREYYPYT 150
    VEKRAEADS 159
    Length:159
    Mass (Da):16,999
    Last modified:June 1, 1998 - v1
    Checksum:i3102BC2ACFA389E7
    GO
    Isoform 2 (identifier: O48528-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1: M → MFNLCALGRTVEEIM

    Note: Derived from EST data. No experimental confirmation available.

    Show »
    Length:173
    Mass (Da):18,577
    Checksum:iA500096BBDC6C4D2
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 11M → MFNLCALGRTVEEIM in isoform 2. CuratedVSP_042321

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ386643 mRNA. Translation: ABD48955.1.
    AC002561 Genomic DNA. Translation: AAB88646.1.
    CP002685 Genomic DNA. Translation: AEC10086.1.
    CP002685 Genomic DNA. Translation: AEC10087.1.
    CP002685 Genomic DNA. Translation: AEC10088.1.
    CP002685 Genomic DNA. Translation: AEC10089.1.
    AY086879 mRNA. Translation: AAM63925.1.
    BT025617 mRNA. Translation: ABF59035.1.
    PIRiT00930.
    RefSeqiNP_001031527.1. NM_001036450.1. [O48528-2]
    NP_001031528.1. NM_001036451.2. [O48528-1]
    NP_001031529.1. NM_001036452.1. [O48528-1]
    NP_565968.1. NM_129783.3. [O48528-1]
    UniGeneiAt.12442.
    At.67859.

    Genome annotation databases

    EnsemblPlantsiAT2G42210.1; AT2G42210.1; AT2G42210. [O48528-1]
    AT2G42210.2; AT2G42210.2; AT2G42210. [O48528-2]
    AT2G42210.3; AT2G42210.3; AT2G42210. [O48528-1]
    AT2G42210.4; AT2G42210.4; AT2G42210. [O48528-1]
    GeneIDi818821.
    KEGGiath:AT2G42210.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ386643 mRNA. Translation: ABD48955.1 .
    AC002561 Genomic DNA. Translation: AAB88646.1 .
    CP002685 Genomic DNA. Translation: AEC10086.1 .
    CP002685 Genomic DNA. Translation: AEC10087.1 .
    CP002685 Genomic DNA. Translation: AEC10088.1 .
    CP002685 Genomic DNA. Translation: AEC10089.1 .
    AY086879 mRNA. Translation: AAM63925.1 .
    BT025617 mRNA. Translation: ABF59035.1 .
    PIRi T00930.
    RefSeqi NP_001031527.1. NM_001036450.1. [O48528-2 ]
    NP_001031528.1. NM_001036451.2. [O48528-1 ]
    NP_001031529.1. NM_001036452.1. [O48528-1 ]
    NP_565968.1. NM_129783.3. [O48528-1 ]
    UniGenei At.12442.
    At.67859.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O48528. 1 interaction.
    STRINGi 3702.AT2G42210.2-P.

    Proteomic databases

    PaxDbi O48528.
    PRIDEi O48528.

    Protocols and materials databases

    DNASUi 818821.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G42210.1 ; AT2G42210.1 ; AT2G42210 . [O48528-1 ]
    AT2G42210.2 ; AT2G42210.2 ; AT2G42210 . [O48528-2 ]
    AT2G42210.3 ; AT2G42210.3 ; AT2G42210 . [O48528-1 ]
    AT2G42210.4 ; AT2G42210.4 ; AT2G42210 . [O48528-1 ]
    GeneIDi 818821.
    KEGGi ath:AT2G42210.

    Organism-specific databases

    TAIRi AT2G42210.

    Phylogenomic databases

    eggNOGi NOG273361.
    HOGENOMi HOG000239422.
    OMAi LYIGVEQ.

    Gene expression databases

    Genevestigatori O48528.

    Family and domain databases

    InterProi IPR003397. Tim17/Tim22/Tim23/PMP24.
    [Graphical view ]
    Pfami PF02466. Tim17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the preprotein and amino acid transporter gene family in Arabidopsis."
      Murcha M.W., Elhafez D., Lister R., Tonti-Filippini J., Baumgartner M., Philippar K., Carrie C., Mokranjac D., Soll J., Whelan J.
      Plant Physiol. 143:199-212(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
      Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    6. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. "Mitochondrial acyl carrier proteins in Arabidopsis thaliana are predominantly soluble matrix proteins and none can be confirmed as subunits of respiratory Complex I."
      Meyer E.H., Heazlewood J.L., Millar A.H.
      Plant Mol. Biol. 64:319-327(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, GENE FAMILY.
    9. "Chloroplast biogenesis: the use of mutants to study the etioplast-chloroplast transition."
      Philippar K., Geis T., Ilkavets I., Oster U., Schwenkert S., Meurer J., Soll J.
      Proc. Natl. Acad. Sci. U.S.A. 104:678-683(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    10. "Resolving and identifying protein components of plant mitochondrial respiratory complexes using three dimensions of gel electrophoresis."
      Meyer E.H., Taylor N.L., Millar A.H.
      J. Proteome Res. 7:786-794(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, SUBCELLULAR LOCATION.
    11. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
      Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
      PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    12. "Defining the protein complex proteome of plant mitochondria."
      Klodmann J., Senkler M., Rode C., Braun H.-P.
      Plant Physiol. 157:587-598(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, SUBCELLULAR LOCATION.
    13. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Dual location of the mitochondrial preprotein transporters B14.7 and Tim23-2 in complex I and the TIM17:23 complex in Arabidopsis links mitochondrial activity and biogenesis."
      Wang Y., Carrie C., Giraud E., Elhafez D., Narsai R., Duncan O., Whelan J., Murcha M.W.
      Plant Cell 24:2675-2695(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TIM23-2, SUBCELLULAR LOCATION, SUBUNIT, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiOP163_ARATH
    AccessioniPrimary (citable) accession number: O48528
    Secondary accession number(s): F4IMZ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 22, 2012
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3