Reviewed, UniProtKB/Swiss-Prot O48500 (DUT_BPT5)

Names and origin

Protein names

Recommended name:
    Deoxyuridine 5'-triphosphate nucleotidohydrolase
      Short name=dUTPase
    EC=3.6.1.23
Alternative name(s):
    dUTP pyrophosphatase

Gene names

Name:

DUT

Organism

Enterobacteria phage T5 (Bacteriophage T5)

Taxonomic identifier

10726 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › T5-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity.
Catalytic activity	dUTP + H2O = dUMP + diphosphate.
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 148	148	Deoxyuridine 5'-triphosphate nucleotidohydrolase		PRO_0000182970

Sequences

	Sequence		Length	Mass (Da)
	O48500-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 4BD56DB1456EF430	FASTA	148	16,204
	10 20 30 40 50 60 MIKIKLTHPD CMPKIGSEDA AGMDLRAFFG TNPAADLRAI APGKSLMIDT GVAVEIPRGW 70 80 90 100 110 120 FGLVVPRSSL GKRHLMIANT AGVIDSDYRG TIKMNLYNYG SEMQTLENFE RLCQLVVLPH 130 140 YSTHNFKIVD ELEETIRGEG GFGSSGSK

References

[1]	"Identification of the bacteriophage T5 dUTPase by protein sequence comparisons." Kaliman A.V. DNA Seq. 6:347-350(1996) [PubMed: 8988373] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY543070 Genomic DNA. Translation: AAS77177.1.
RefSeq	YP_006959.1.
3D structure databases
HSSP	HSSP built from PDB template 1F7D based on UniProtKB P16088.
ModBase	Search...
Genome annotation databases
GeneID	2777637.
GenomeReviews	Gene locus T5.132 in contig AY543070_GR.
Enzyme and pathway databases
BRENDA	3.6.1.23. 260010.
Family and domain databases
InterPro	IPR008180. DeoxyUTP_pyroPase_dom. IPR008181. dUTP_pyrophosphatase_subfam_1. [Graphical view]
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
ProDom	PD004900. dCTP_deaminase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_BPT5

Accession

Primary (citable) accession number: O48500

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	September 22, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)