Reviewed, UniProtKB/Swiss-Prot O47950 (NDUS7_NEUCR)

Names and origin

Protein names

Recommended name:
    NADH-ubiquinone oxidoreductase 19.3 kDa subunit, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    Complex I-19.3kD
      Short name=CI-19.3kD

Gene names

ORF Names:

G17A4.170, NCU03953

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

5141 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Catalytic activity	NADH + ubiquinone = NAD(+) + ubiquinol. NADH + acceptor = NAD(+) + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster Potential.
Subunit structure	Complex I is composed of about 40 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 226		NADH-ubiquinone oxidoreductase 19.3 kDa subunit, mitochondrial		PRO_0000020032
Sites
	Metal binding	101	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	102	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	166	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	196	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	O47950-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: E48CB09C64AAAFC6	FASTA	226	24,972
	10 20 30 40 50 60 MMSSVRTGAS MALRARPTAQ IVPFRAAAVA SISSSSRKDA TGAVAPAGAQ HGIARRERRE 70 80 90 100 110 120 VPLPSQEGTK GAVQYALTTL DSIVNWARQS SLWPMTFGLA CCAVEMMHLS TPRYDQDRLG 130 140 150 160 170 180 IIFRASPRQS DVMIVAGTLT NKMAPALRQV YDQMPDPRWV ISMGSCANGG GYYHYSYSVV 190 200 210 220 RGCDRIVPVD IYVPGCPPTS EALMYGIFQL QRKMRNTKIT RMWYRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterisation of the last Fe-S cluster-binding subunit of Neurospora crassa complex I." Sousa R., Barquera B., Duarte M., Finel M., Videira A. Biochim. Biophys. Acta 1411:142-146(1999) [PubMed: 10216160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ001520 mRNA. Translation: CAA04802.1. BX908812 Genomic DNA. Translation: CAF06152.1. AABX02000012 Genomic DNA. Translation: EAA28356.1.
RefSeq	XP_957592.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3873682.
KEGG	ncr:NCU03953.
NMPDR	fig|5141.1.peg.1205.
Enzyme and pathway databases
BioCyc	NCRA-XX3-01:NCRA-XX3-01-007701-MON.
Family and domain databases
InterPro	IPR006138. NADH_DHase_20kDa_su. IPR014406. NiFe_hyd_3_ssu/Q_oxred_NuoB. IPR006137. OxRdtase_q6. [Graphical view]
PANTHER	PTHR11995:SF2. NADH_DH_20kDa. 1 hit. PTHR11995. NiFe_hyd_3_ssu/Q_oxred_NuoB. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR01957. nuoB_fam. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS7_NEUCR

Accession

Primary (citable) accession number: O47950
Secondary accession number(s): Q7RZB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	November 25, 2008
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)