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O47950

- NDUS7_NEUCR

UniProt

O47950 - NDUS7_NEUCR

Protein

NADH-ubiquinone oxidoreductase 19.3 kDa subunit, mitochondrial

Gene

G17A4.170

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 4Fe-4S cluster.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi101 – 1011Iron-sulfur (4Fe-4S)Sequence Analysis
    Metal bindingi102 – 1021Iron-sulfur (4Fe-4S)Sequence Analysis
    Metal bindingi166 – 1661Iron-sulfur (4Fe-4S)Sequence Analysis
    Metal bindingi196 – 1961Iron-sulfur (4Fe-4S)Sequence Analysis

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
    4. quinone binding Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Protein family/group databases

    TCDBi3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-ubiquinone oxidoreductase 19.3 kDa subunit, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-19.3kD
    Short name:
    CI-19.3kD
    Gene namesi
    ORF Names:G17A4.170, NCU03953
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 5, Linkage Group VI

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell
    2. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 226NADH-ubiquinone oxidoreductase 19.3 kDa subunit, mitochondrialPRO_0000020032
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Interactioni

    Subunit structurei

    Complex I is composed of about 40 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.

    Protein-protein interaction databases

    STRINGi5141.NCU03953.1.

    Structurei

    3D structure databases

    ProteinModelPortaliO47950.
    SMRiO47950. Positions 75-220.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 20 kDa subunit family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0377.
    HOGENOMiHOG000228249.
    KOiK03940.
    OMAiINWARNG.
    OrthoDBiEOG7FBRVF.

    Family and domain databases

    Gene3Di3.40.50.700. 1 hit.
    HAMAPiMF_01356. NDH1_NuoB.
    InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    IPR006138. NADH_UQ_OxRdtase_20Kd_su.
    [Graphical view]
    PfamiPF01058. Oxidored_q6. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
    PROSITEiPS01150. COMPLEX1_20K. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O47950-1 [UniParc]FASTAAdd to Basket

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    MMSSVRTGAS MALRARPTAQ IVPFRAAAVA SISSSSRKDA TGAVAPAGAQ    50
    HGIARRERRE VPLPSQEGTK GAVQYALTTL DSIVNWARQS SLWPMTFGLA 100
    CCAVEMMHLS TPRYDQDRLG IIFRASPRQS DVMIVAGTLT NKMAPALRQV 150
    YDQMPDPRWV ISMGSCANGG GYYHYSYSVV RGCDRIVPVD IYVPGCPPTS 200
    EALMYGIFQL QRKMRNTKIT RMWYRK 226
    Length:226
    Mass (Da):24,972
    Last modified:June 1, 1998 - v1
    Checksum:iE48CB09C64AAAFC6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001520 mRNA. Translation: CAA04802.1.
    BX908812 Genomic DNA. Translation: CAF06152.1.
    CM002241 Genomic DNA. Translation: EAA28356.1.
    RefSeqiXP_957592.1. XM_952499.2.
    UniGeneiNcr.7975.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000003665; EFNCRP00000003665; EFNCRG00000003661.
    GeneIDi3873682.
    KEGGincr:NCU03953.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ001520 mRNA. Translation: CAA04802.1 .
    BX908812 Genomic DNA. Translation: CAF06152.1 .
    CM002241 Genomic DNA. Translation: EAA28356.1 .
    RefSeqi XP_957592.1. XM_952499.2.
    UniGenei Ncr.7975.

    3D structure databases

    ProteinModelPortali O47950.
    SMRi O47950. Positions 75-220.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU03953.1.

    Protein family/group databases

    TCDBi 3.D.1.6.2. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000003665 ; EFNCRP00000003665 ; EFNCRG00000003661 .
    GeneIDi 3873682.
    KEGGi ncr:NCU03953.

    Phylogenomic databases

    eggNOGi COG0377.
    HOGENOMi HOG000228249.
    KOi K03940.
    OMAi INWARNG.
    OrthoDBi EOG7FBRVF.

    Family and domain databases

    Gene3Di 3.40.50.700. 1 hit.
    HAMAPi MF_01356. NDH1_NuoB.
    InterProi IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    IPR006138. NADH_UQ_OxRdtase_20Kd_su.
    [Graphical view ]
    Pfami PF01058. Oxidored_q6. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01957. nuoB_fam. 1 hit.
    PROSITEi PS01150. COMPLEX1_20K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterisation of the last Fe-S cluster-binding subunit of Neurospora crassa complex I."
      Sousa R., Barquera B., Duarte M., Finel M., Videira A.
      Biochim. Biophys. Acta 1411:142-146(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
      Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
      Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    3. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

    Entry informationi

    Entry nameiNDUS7_NEUCR
    AccessioniPrimary (citable) accession number: O47950
    Secondary accession number(s): Q7RZB4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3