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Protein

Nitric oxide synthase, inducible

Gene

NOS2

Organism
Macaca mulatta (Rhesus macaque)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. INOS and NO may contribute to simian immunodeficiency virus (SIV)-induced central nervous system disease. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 (By similarity).By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarity
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • FMNBy similarityNote: Binds 1 FMN.By similarity
  • 5,6,7,8-tetrahydrobiopterinBy similarityNote: Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.By similarity

Enzyme regulationi

Regulated by calcium/calmodulin.By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
  2. peptidyl-cysteine S-nitrosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calcium, Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:NOS2
OrganismiMacaca mulatta (Rhesus macaque)
Taxonomic identifieri9544 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca
ProteomesiUP000006718 Componenti: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›162›162Nitric oxide synthase, induciblePRO_0000170933Add
BLAST

Expressioni

Inductioni

By lipopolysaccharide (LPS).

Interactioni

Subunit structurei

Homodimer. Binds SLC9A3R1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO46660.
SMRiO46660. Positions 63-162.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NOS family.Curated

Phylogenomic databases

InParanoidiO46660.

Family and domain databases

Gene3Di3.90.340.10. 1 hit.
InterProiIPR004030. NOS_N.
[Graphical view]
SUPFAMiSSF56512. SSF56512. 1 hit.

Sequencei

Sequence statusi: Fragment.

O46660-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GKDINNNMEK AACATSSLVT QDDLQYHSLS KQQNESPQPL VGTGKKSPES
60 70 80 90 100
LVKPDATPLS SPRHVRIKNW GSGMTFQDTL HHKAKGILTC RSKSCLGSIM
110 120 130 140 150
TPKSLTRGPR DKPTPPDELL PQAIEFVNLS LEISVQDQIP PVCNDCQYYG
160
SFKEAKIEEH LA
Length:162
Mass (Da):17,813
Last modified:June 1, 1998 - v1
Checksum:i0457464D32287065
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei162 – 1621

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31907 mRNA. Translation: AAC39525.1.
UniGeneiMmu.3443.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31907 mRNA. Translation: AAC39525.1.
UniGeneiMmu.3443.

3D structure databases

ProteinModelPortaliO46660.
SMRiO46660. Positions 63-162.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

InParanoidiO46660.

Family and domain databases

Gene3Di3.90.340.10. 1 hit.
InterProiIPR004030. NOS_N.
[Graphical view]
SUPFAMiSSF56512. SSF56512. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Expression of inflammatory cytokines and inducible nitric oxide synthase in brains of SIV-infected rhesus monkeys: applications to HIV-induced central nervous system disease."
    Lane T.E., Buchmeier M.J., Watry D.D., Fox H.S.
    Mol. Med. 2:27-37(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Microglia.

Entry informationi

Entry nameiNOS2_MACMU
AccessioniPrimary (citable) accession number: O46660
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: January 7, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.