O46660 (NOS2_MACMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitric oxide synthase, inducible
EC=1.14.13.39

Alternative name(s):
Inducible NO synthase
Short name=Inducible NOS
Short name=iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2

Gene names

Name:

NOS2

Organism

Macaca mulatta (Rhesus macaque) [Reference proteome]

Taxonomic identifier

9544 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Cercopithecoidea › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	162 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. INOS and NO may contribute to simian immunodeficiency virus (SIV)-induced central nervous system disease. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.
Catalytic activity	2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.
Enzyme regulation	Regulated by calcium/calmodulin By similarity.
Subunit structure	Homodimer. Binds SLC9A3R1 By similarity.
Induction	By lipopolysaccharide (LPS).
Sequence similarities	Belongs to the NOS family.

Ontologies

Keywords
Ligand	Calcium Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro peptidyl-cysteine S-nitrosylation Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitric-oxide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›162	›162	Nitric oxide synthase, inducible		PRO_0000170933
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	162	1

Sequences

	Sequence		Length	Mass (Da)
	O46660 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 0457464D32287065	FASTA	162	17,813
	10 20 30 40 50 60 GKDINNNMEK AACATSSLVT QDDLQYHSLS KQQNESPQPL VGTGKKSPES LVKPDATPLS 70 80 90 100 110 120 SPRHVRIKNW GSGMTFQDTL HHKAKGILTC RSKSCLGSIM TPKSLTRGPR DKPTPPDELL 130 140 150 160 PQAIEFVNLS LEISVQDQIP PVCNDCQYYG SFKEAKIEEH LA

References

[1]

"Expression of inflammatory cytokines and inducible nitric oxide synthase in brains of SIV-infected rhesus monkeys: applications to HIV-induced central nervous system disease." Lane T.E., Buchmeier M.J., Watry D.D., Fox H.S. Mol. Med. 2:27-37(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Microglia.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U31907 mRNA. Translation: AAC39525.1.
UniGene	Mmu.3443.
3D structure databases
ProteinModelPortal	O46660.
SMR	O46660. Positions 63-162.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.90.340.10. 1 hit.
InterPro	IPR004030. NO_synthase_oxygenase_dom. [Graphical view]
SUPFAM	SSF56512. NO_synthase_oxygenase_reg. 1 hit.
PROSITE	PS60001. NOS. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOS2_MACMU

Accession

Primary (citable) accession number: O46660

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program