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Reviewed, UniProtKB/Swiss-Prot O46658 (CP2DP_PIG)

Last modified June 16, 2009. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Vitamin D(3) 25-hydroxylase
    EC=1.14.13.15
Alternative name(s):
    Cytochrome P450 2D25
    CYPIID25
Gene names
Name: CYP2D25
OrganismSus scrofa (Pig)
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Catalyzes the first step in the metabolic activation of vitamin D3 into 1-alpha,25-dihydroxyvitamin D3, its active, hormonal form.

Catalytic activity

5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol + NADPH + O2 = (25R)-5-beta-cholestane-3-alpha,7-alpha,12-alpha,26-tetraol + NADP+ + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Found in liver and kidney.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1 Ref.2 Ref.3
Chain2 – 500499Vitamin D(3) 25-hydroxylase
PRO_0000051745

Sites

Metal binding4461Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
O46658-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: F1C878A02C44503D

FASTA50056,512
        10         20         30         40         50         60 
MGLLTGDLLG ILALAMVIFL LLVDLMHRRS RWAPRYPPGP MPLPGLGNLL QVNFQDPRLS 

        70         80         90        100        110        120 
FIQLRRRFGD VFSLQQIWRP VVVLNGLAAV REALVSHSHE TSDRPPVFIL EHLGYGPRSE 

       130        140        150        160        170        180 
GVILARYGKA WREQRRFSVS TLRNFGLGKK SLEEWVTQEA SCLCAAFADQ AGRPFSPNNL 

       190        200        210        220        230        240 
LNKAVSNVIA SLTFARRFEY NDPRMLKLLD LVLEGLKEEV GLMRQVLEAM PVLRHIPGLC 

       250        260        270        280        290        300 
AKLFPRQKAF LVMIDELITE HKMTRDLAQP PRDLTDAFLD EMKEAKGNPE SSFNDENLRL 

       310        320        330        340        350        360 
VVAHLFSAGM ITTSTTLAWA LLLMILHPDV QRRVQQEIDE VIGHVRQPEI KDQALMPFTL 

       370        380        390        400        410        420 
AVLHEVQRFG DIVPLGVAHM TSCDIEVQGF LIPKGTTLIT NLTSVLKDET VWKKPFRFYP 

       430        440        450        460        470        480 
EHFLDAQGRF TKQEAFMPFS AGRRSCLGEP LARMELFLFF TTLLQAFSFS VPTGQPCPSD 

       490        500 
HGVFAFLLFP SPYQLCAVPR 

« Hide

References

[1]"Cloning, structure, and expression of a cDNA encoding vitamin D3 25-hydroxylase."
Postlind H., Axen E., Bergman T., Wikvall K.
Biochem. Biophys. Res. Commun. 241:491-497(1997) [PubMed: 9425298] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-57; 249-273 AND 408-430.
Tissue: Liver.
[2]"Purification and characterization of a vitamin D3 25-hydroxylase from pig liver microsomes."
Axen E., Bergman T., Wikvall K.
Biochem. J. 287:725-731(1992) [PubMed: 1445236] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-17.
Tissue: Liver.
[3]"Purification and characterization of cytochrome P-45014DM (lanosterol 14 alpha-demethylase) from pig liver microsomes."
Sono H., Sonoda Y., Sato Y.
Biochim. Biophys. Acta 1078:388-394(1991) [PubMed: 1859829] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-11.
Tissue: Liver.

Cross-references

Sequence databases

Y16417 mRNA. Translation: CAA76205.1.
PIRJC5819.
RefSeqNP_999559.1.
UniGeneSsc.55051

3D structure databases

HSSPHSSP built from PDB template 1DT6 based on UniProtKB P00179.
ModBaseSearch...

Genome annotation databases

GeneID397687.
KEGGssc:397687.

Phylogenomic databases

HOVERGENO46658.

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR017973. Cyt_P450_C.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHERPTHR19383. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP2DP_PIG
AccessionPrimary (citable) accession number: O46658
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 62 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents