Reviewed, UniProtKB/Swiss-Prot O46658 (CP2DP_PIG)

Names and origin

Protein names

Recommended name:
    Vitamin D(3) 25-hydroxylase
    EC=1.14.13.15
Alternative name(s):
    Cytochrome P450 2D25
    CYPIID25

Gene names

Name:

CYP2D25

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the first step in the metabolic activation of vitamin D3 into 1-alpha,25-dihydroxyvitamin D3, its active, hormonal form.
Catalytic activity	5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol + NADPH + O2 = (25R)-5-beta-cholestane-3-alpha,7-alpha,12-alpha,26-tetraol + NADP+ + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Found in liver and kidney.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cholestanetriol 26-monooxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1 Ref.2 Ref.3
	Chain	2 – 500	499	Vitamin D(3) 25-hydroxylase		PRO_0000051745
Sites
	Metal binding	446	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O46658-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F1C878A02C44503D	FASTA	500	56,512
	10 20 30 40 50 60 MGLLTGDLLG ILALAMVIFL LLVDLMHRRS RWAPRYPPGP MPLPGLGNLL QVNFQDPRLS 70 80 90 100 110 120 FIQLRRRFGD VFSLQQIWRP VVVLNGLAAV REALVSHSHE TSDRPPVFIL EHLGYGPRSE 130 140 150 160 170 180 GVILARYGKA WREQRRFSVS TLRNFGLGKK SLEEWVTQEA SCLCAAFADQ AGRPFSPNNL 190 200 210 220 230 240 LNKAVSNVIA SLTFARRFEY NDPRMLKLLD LVLEGLKEEV GLMRQVLEAM PVLRHIPGLC 250 260 270 280 290 300 AKLFPRQKAF LVMIDELITE HKMTRDLAQP PRDLTDAFLD EMKEAKGNPE SSFNDENLRL 310 320 330 340 350 360 VVAHLFSAGM ITTSTTLAWA LLLMILHPDV QRRVQQEIDE VIGHVRQPEI KDQALMPFTL 370 380 390 400 410 420 AVLHEVQRFG DIVPLGVAHM TSCDIEVQGF LIPKGTTLIT NLTSVLKDET VWKKPFRFYP 430 440 450 460 470 480 EHFLDAQGRF TKQEAFMPFS AGRRSCLGEP LARMELFLFF TTLLQAFSFS VPTGQPCPSD 490 500 HGVFAFLLFP SPYQLCAVPR

References

[1]	"Cloning, structure, and expression of a cDNA encoding vitamin D3 25-hydroxylase." Postlind H., Axen E., Bergman T., Wikvall K. Biochem. Biophys. Res. Commun. 241:491-497(1997) [PubMed: 9425298] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-57; 249-273 AND 408-430. Tissue: Liver.
[2]	"Purification and characterization of a vitamin D3 25-hydroxylase from pig liver microsomes." Axen E., Bergman T., Wikvall K. Biochem. J. 287:725-731(1992) [PubMed: 1445236] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-17. Tissue: Liver.
[3]	"Purification and characterization of cytochrome P-45014DM (lanosterol 14 alpha-demethylase) from pig liver microsomes." Sono H., Sonoda Y., Sato Y. Biochim. Biophys. Acta 1078:388-394(1991) [PubMed: 1859829] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y16417 mRNA. Translation: CAA76205.1.
PIR	JC5819.
RefSeq	NP_999559.1.
UniGene	Ssc.55051
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
ModBase	Search...
Genome annotation databases
GeneID	397687.
KEGG	ssc:397687.
Phylogenomic databases
HOVERGEN	O46658.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008069. Cyt_P450_E_grp-I_CYP2D-like. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01686. EP450ICYP2D. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP2DP_PIG

Accession

Primary (citable) accession number: O46658

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 62 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)