Reviewed, UniProtKB/Swiss-Prot O46644 (ELA1_MACFA)

Names and origin

Protein names

Recommended name:
    Elastase-1
    EC=3.4.21.36

Gene names

Name:

ELA1

Organism

Macaca fascicularis (Crab eating macaque) (Cynomolgus monkey)

Taxonomic identifier

9541 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts upon elastin By similarity.
Catalytic activity	Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 26	10	Activation peptide		PRO_0000027679
	Chain	27 – 266	240	Elastase-1		PRO_0000027680
Regions
	Domain	27 – 264	238	Peptidase S1
Sites
	Active site	71	1	Charge relay system By similarity
	Active site	119	1	Charge relay system By similarity
	Active site	214	1	Charge relay system By similarity
	Metal binding	85	1	Calcium By similarity
	Metal binding	90	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	95	1	Calcium By similarity
Amino acid modifications
	Glycosylation	87	1	N-linked (GlcNAc...) Potential
	Glycosylation	241	1	N-linked (GlcNAc...) Potential
	Glycosylation	260	1	N-linked (GlcNAc...) Potential
	Disulfide bond	56 ↔ 72		By similarity
	Disulfide bond	153 ↔ 220		By similarity
	Disulfide bond	184 ↔ 200		By similarity
	Disulfide bond	210 ↔ 240		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O46644-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 6B3281B90F53A91C	FASTA	266	28,754
	10 20 30 40 50 60 MLRFLVFATL VLYGHSTQDF PETNARVVGG TEAGRNSWPS QISLQYLSGG SWYHTCGGTL 70 80 90 100 110 120 IRQNWVMTAA HCVDSPKTFR VVVGDHNLSQ NDGTEQYVSV QKIVVHPYWN SNNVAAGYDI 130 140 150 160 170 180 ALLRLAQSVT LNSYVQLGVL PQEGAILAND SPCYITGWGR TKTNGQLAQT LQQAYLPSVD 190 200 210 220 230 240 YAICSSSSYW GSTVKNTMVC AGGDGVHSGC QGDSGGPLHC LVNGKYSVHG VTSFVSKQGC 250 260 NVSRKPTVFT RVSAYISWIN KTIASN

References

[1]	Kawashima I. Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X91400 mRNA. Translation: CAA62746.1.
3D structure databases
HSSP	HSSP built from PDB template 1GVK based on UniProtKB P00772.
SMR	O46644. Positions 27-266.
ModBase	Search...
Protein family/group databases
MEROPS	S01.153.
Phylogenomic databases
HOVERGEN	O46644.
Enzyme and pathway databases
BRENDA	3.4.21.36. 3438.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ELA1_MACFA

Accession

Primary (citable) accession number: O46644

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)