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O46635

- 5HT2A_CANFA

UniProt

O46635 - 5HT2A_CANFA

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Protein

5-hydroxytryptamine receptor 2A

Gene

HTR2A

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.1 Publication

GO - Molecular functioni

  1. drug binding Source: UniProtKB
  2. serotonin binding Source: UniProtKB
  3. serotonin receptor activity Source: UniProtKB

GO - Biological processi

  1. behavior Source: UniProtKB-KW
  2. regulation of behavior Source: InterPro
  3. regulation of hormone secretion Source: InterPro
  4. release of sequestered calcium ion into cytosol Source: UniProtKB
  5. response to drug Source: UniProtKB
  6. serotonin receptor signaling pathway Source: GOC
  7. smooth muscle contraction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Behavior

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 2A
Short name:
5-HT-2
Short name:
5-HT-2A
Short name:
5-hydroxytryptamine receptor
Alternative name(s):
Serotonin receptor 2A
Gene namesi
Name:HTR2A
Synonyms:5-HTR2A
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254: Unplaced

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell projectiondendrite By similarity. Cell projectionaxon By similarity. Cytoplasmic vesicle By similarity. Membranecaveola By similarity
Note: Localizes to the postsynaptic thickening of axo-dendritic synapses.By similarity

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB-KW
  2. dendritic shaft Source: InterPro
  3. integral component of plasma membrane Source: InterPro
  4. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4704705-hydroxytryptamine receptor 2APRO_0000068943Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi51 – 511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi54 – 541N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi148 ↔ 227PROSITE-ProRule annotation
Modified residuei280 – 2801PhosphoserineBy similarity
Disulfide bondi348 ↔ 352PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Interactioni

Subunit structurei

Interacts with MPDZ and INADL. May interact with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2. Interacts with GRM2 and DRD2; this may affect signaling (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliO46635.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7575ExtracellularBy similarityAdd
BLAST
Topological domaini100 – 11011CytoplasmicBy similarityAdd
BLAST
Topological domaini133 – 14816ExtracellularBy similarityAdd
BLAST
Topological domaini172 – 19120CytoplasmicBy similarityAdd
BLAST
Topological domaini216 – 23318ExtracellularBy similarityAdd
BLAST
Topological domaini255 – 32369CytoplasmicBy similarityAdd
BLAST
Topological domaini346 – 36116ExtracellularBy similarityAdd
BLAST
Topological domaini384 – 47087CytoplasmicBy similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei76 – 9924Helical; Name=1By similarityAdd
BLAST
Transmembranei111 – 13222Helical; Name=2By similarityAdd
BLAST
Transmembranei149 – 17123Helical; Name=3By similarityAdd
BLAST
Transmembranei192 – 21524Helical; Name=4By similarityAdd
BLAST
Transmembranei234 – 25421Helical; Name=5By similarityAdd
BLAST
Transmembranei324 – 34522Helical; Name=6By similarityAdd
BLAST
Transmembranei362 – 38322Helical; Name=7By similarityAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni155 – 1606Agonist bindingBy similarity
Regioni335 – 3395Agonist bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi172 – 1743DRY motif; important for ligand-induced conformation changesBy similarity
Motifi375 – 3795NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity
Motifi468 – 4703PDZ-binding

Domaini

The PDZ domain-binding motif is involved in the interaction with INADL, CASK, APBA1, DLG1 and DLG4.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG247243.
HOGENOMiHOG000240378.
HOVERGENiHBG107487.
InParanoidiO46635.
KOiK04157.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR000455. 5HT2A_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24247:SF30. PTHR24247:SF30. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00516. 5HT2ARECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O46635-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDVLFEDNAP LSPTTSSLMP SNGDPRLYGN DLNAGDANTS DAFNWTVDAE
60 70 80 90 100
NRTNLSCEGC LSPPCFSLLH LQEKNWSALL TAVVIILTIA GNILVIMAVS
110 120 130 140 150
LEKKLQNATN YFLMSLAIAD MLLGFLVMPV SMLTILYGYR WPLPSKLCAV
160 170 180 190 200
WIYLDVLFST ASIMHLCAIS LDRYVAIQNP IHHSRFNSRT KAFLKIIAVW
210 220 230 240 250
TISVGISMPI PVFGLQDDSK VFKEGSCLLA DDNFVLIGSF VSFFIPLTIM
260 270 280 290 300
VITYFLTIKS LQKEATLCVS DPGTRAKLAS FSFLPQSSLS SEKLFQRSIH
310 320 330 340 350
REPGSYGRRT MQSISNEQKA CKVLGIVFFL FVVMWCPFFI TNIMAVICKE
360 370 380 390 400
SCNEDIIGAL LNVFVWIGYL SSAVNPLVYT LFNKTYRSAF SRYIQCQYKE
410 420 430 440 450
NKKPLQLILV NTIPALAYKS SQLQMGQKKN SKKDAKSTDN DYSMVALGKQ
460 470
HSEDAPTDNI NTVNEKVSCV
Length:470
Mass (Da):52,378
Last modified:April 12, 2005 - v2
Checksum:i5C654F81E88B92E3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB193092 mRNA. Translation: BAD60922.1.
AY832858 mRNA. Translation: AAX39385.1.
Y16134 mRNA. Translation: CAA76080.1.
RefSeqiNP_001005869.1. NM_001005869.1.
UniGeneiCfa.8880.

Genome annotation databases

GeneIDi403882.
KEGGicfa:403882.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB193092 mRNA. Translation: BAD60922.1 .
AY832858 mRNA. Translation: AAX39385.1 .
Y16134 mRNA. Translation: CAA76080.1 .
RefSeqi NP_001005869.1. NM_001005869.1.
UniGenei Cfa.8880.

3D structure databases

ProteinModelPortali O46635.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi O46635.
ChEMBLi CHEMBL5781.

Protein family/group databases

GPCRDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 403882.
KEGGi cfa:403882.

Organism-specific databases

CTDi 3356.

Phylogenomic databases

eggNOGi NOG247243.
HOGENOMi HOG000240378.
HOVERGENi HBG107487.
InParanoidi O46635.
KOi K04157.

Miscellaneous databases

NextBioi 20817374.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR000455. 5HT2A_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view ]
PANTHERi PTHR24247:SF30. PTHR24247:SF30. 1 hit.
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00516. 5HT2ARECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequencing of canine 5-hydroxytriptamine receptor (5-HTR) 1B, 2A, 2C genes and identification of polymorphisms in the 5-HTR1B gene."
    Masuda K., Hashizume C., Ogata N., Kikusui T., Takeuchi Y., Mori Y.
    J. Vet. Med. Sci. 66:965-972(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Beagle.
  2. "Molecular and pharmacological characterization of serotonin 5-HT(2A) and 5-HT(2B) receptor subtypes in dog."
    Bonaventure P., Nepomuceno D., Miller K., Chen J., Kuei C., Kamme F., Tran D.T., Lovenberg T.W., Liu C.
    Eur. J. Pharmacol. 513:181-192(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. Sgard F.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 120-307.
    Tissue: Femoral artery.

Entry informationi

Entry namei5HT2A_CANFA
AccessioniPrimary (citable) accession number: O46635
Secondary accession number(s): Q50DZ9, Q60F96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: April 12, 2005
Last modified: October 29, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3