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O46635

- 5HT2A_CANFA

UniProt

O46635 - 5HT2A_CANFA

Protein

5-hydroxytryptamine receptor 2A

Gene

HTR2A

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 2 (12 Apr 2005)
      Previous versions | rss
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    Functioni

    G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.1 Publication

    GO - Molecular functioni

    1. drug binding Source: UniProtKB
    2. serotonin binding Source: UniProtKB
    3. serotonin receptor activity Source: UniProtKB

    GO - Biological processi

    1. behavior Source: UniProtKB-KW
    2. regulation of behavior Source: InterPro
    3. regulation of hormone secretion Source: InterPro
    4. release of sequestered calcium ion into cytosol Source: UniProtKB
    5. response to drug Source: UniProtKB
    6. serotonin receptor signaling pathway Source: GOC
    7. smooth muscle contraction Source: InterPro

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Keywords - Biological processi

    Behavior

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-hydroxytryptamine receptor 2A
    Short name:
    5-HT-2
    Short name:
    5-HT-2A
    Short name:
    5-hydroxytryptamine receptor
    Alternative name(s):
    Serotonin receptor 2A
    Gene namesi
    Name:HTR2A
    Synonyms:5-HTR2A
    OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
    Taxonomic identifieri9615 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
    ProteomesiUP000002254: Unplaced

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication. Cell projectiondendrite By similarity. Cell projectionaxon By similarity. Cytoplasmic vesicle By similarity. Membranecaveola By similarity
    Note: Localizes to the postsynaptic thickening of axo-dendritic synapses.By similarity

    GO - Cellular componenti

    1. axon Source: UniProtKB-SubCell
    2. caveola Source: UniProtKB-SubCell
    3. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
    4. dendritic shaft Source: InterPro
    5. integral component of plasma membrane Source: InterPro
    6. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cytoplasmic vesicle, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4704705-hydroxytryptamine receptor 2APRO_0000068943Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi38 – 381N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi51 – 511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi54 – 541N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi148 ↔ 227PROSITE-ProRule annotation
    Disulfide bondi348 ↔ 352PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Ubiquitous.1 Publication

    Interactioni

    Subunit structurei

    Interacts with MPDZ and INADL. May interact with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2. Interacts with GRM2 and DRD2; this may affect signaling By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO46635.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7575ExtracellularBy similarityAdd
    BLAST
    Topological domaini100 – 11011CytoplasmicBy similarityAdd
    BLAST
    Topological domaini133 – 14816ExtracellularBy similarityAdd
    BLAST
    Topological domaini172 – 19120CytoplasmicBy similarityAdd
    BLAST
    Topological domaini216 – 23318ExtracellularBy similarityAdd
    BLAST
    Topological domaini255 – 32369CytoplasmicBy similarityAdd
    BLAST
    Topological domaini346 – 36116ExtracellularBy similarityAdd
    BLAST
    Topological domaini384 – 47087CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei76 – 9924Helical; Name=1By similarityAdd
    BLAST
    Transmembranei111 – 13222Helical; Name=2By similarityAdd
    BLAST
    Transmembranei149 – 17123Helical; Name=3By similarityAdd
    BLAST
    Transmembranei192 – 21524Helical; Name=4By similarityAdd
    BLAST
    Transmembranei234 – 25421Helical; Name=5By similarityAdd
    BLAST
    Transmembranei324 – 34522Helical; Name=6By similarityAdd
    BLAST
    Transmembranei362 – 38322Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni155 – 1606Agonist bindingBy similarity
    Regioni335 – 3395Agonist bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi172 – 1743DRY motif; important for ligand-induced conformation changesBy similarity
    Motifi375 – 3795NPxxY motif; important for ligand-induced conformation changes and signalingBy similarity
    Motifi468 – 4703PDZ-binding

    Domaini

    The PDZ domain-binding motif is involved in the interaction with INADL, CASK, APBA1, DLG1 and DLG4.By similarity

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG247243.
    HOGENOMiHOG000240378.
    HOVERGENiHBG107487.
    InParanoidiO46635.
    KOiK04157.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000455. 5HT2A_rcpt.
    IPR002231. 5HT_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PANTHERiPTHR24247:SF30. PTHR24247:SF30. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00516. 5HT2ARECEPTR.
    PR01101. 5HTRECEPTOR.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O46635-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDVLFEDNAP LSPTTSSLMP SNGDPRLYGN DLNAGDANTS DAFNWTVDAE    50
    NRTNLSCEGC LSPPCFSLLH LQEKNWSALL TAVVIILTIA GNILVIMAVS 100
    LEKKLQNATN YFLMSLAIAD MLLGFLVMPV SMLTILYGYR WPLPSKLCAV 150
    WIYLDVLFST ASIMHLCAIS LDRYVAIQNP IHHSRFNSRT KAFLKIIAVW 200
    TISVGISMPI PVFGLQDDSK VFKEGSCLLA DDNFVLIGSF VSFFIPLTIM 250
    VITYFLTIKS LQKEATLCVS DPGTRAKLAS FSFLPQSSLS SEKLFQRSIH 300
    REPGSYGRRT MQSISNEQKA CKVLGIVFFL FVVMWCPFFI TNIMAVICKE 350
    SCNEDIIGAL LNVFVWIGYL SSAVNPLVYT LFNKTYRSAF SRYIQCQYKE 400
    NKKPLQLILV NTIPALAYKS SQLQMGQKKN SKKDAKSTDN DYSMVALGKQ 450
    HSEDAPTDNI NTVNEKVSCV 470
    Length:470
    Mass (Da):52,378
    Last modified:April 12, 2005 - v2
    Checksum:i5C654F81E88B92E3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB193092 mRNA. Translation: BAD60922.1.
    AY832858 mRNA. Translation: AAX39385.1.
    Y16134 mRNA. Translation: CAA76080.1.
    RefSeqiNP_001005869.1. NM_001005869.1.
    UniGeneiCfa.8880.

    Genome annotation databases

    GeneIDi403882.
    KEGGicfa:403882.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB193092 mRNA. Translation: BAD60922.1 .
    AY832858 mRNA. Translation: AAX39385.1 .
    Y16134 mRNA. Translation: CAA76080.1 .
    RefSeqi NP_001005869.1. NM_001005869.1.
    UniGenei Cfa.8880.

    3D structure databases

    ProteinModelPortali O46635.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi O46635.
    ChEMBLi CHEMBL5781.

    Protein family/group databases

    GPCRDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 403882.
    KEGGi cfa:403882.

    Organism-specific databases

    CTDi 3356.

    Phylogenomic databases

    eggNOGi NOG247243.
    HOGENOMi HOG000240378.
    HOVERGENi HBG107487.
    InParanoidi O46635.
    KOi K04157.

    Miscellaneous databases

    NextBioi 20817374.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000455. 5HT2A_rcpt.
    IPR002231. 5HT_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    PANTHERi PTHR24247:SF30. PTHR24247:SF30. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00516. 5HT2ARECEPTR.
    PR01101. 5HTRECEPTOR.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing of canine 5-hydroxytriptamine receptor (5-HTR) 1B, 2A, 2C genes and identification of polymorphisms in the 5-HTR1B gene."
      Masuda K., Hashizume C., Ogata N., Kikusui T., Takeuchi Y., Mori Y.
      J. Vet. Med. Sci. 66:965-972(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Beagle.
    2. "Molecular and pharmacological characterization of serotonin 5-HT(2A) and 5-HT(2B) receptor subtypes in dog."
      Bonaventure P., Nepomuceno D., Miller K., Chen J., Kuei C., Kamme F., Tran D.T., Lovenberg T.W., Liu C.
      Eur. J. Pharmacol. 513:181-192(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    3. Sgard F.
      Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 120-307.
      Tissue: Femoral artery.

    Entry informationi

    Entry namei5HT2A_CANFA
    AccessioniPrimary (citable) accession number: O46635
    Secondary accession number(s): Q50DZ9, Q60F96
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: April 12, 2005
    Last modified: October 1, 2014
    This is version 97 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3