Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O46635 (5HT2A_CANFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-hydroxytryptamine receptor 2A

Short name=5-HT-2
Short name=5-HT-2A
Short name=5-hydroxytryptamine receptor
Alternative name(s):
Serotonin receptor 2A
Gene names
Name:HTR2A
Synonyms:5-HTR2A
OrganismCanis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]
Taxonomic identifier9615 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis

Protein attributes

Sequence length470 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction. Ref.2

Subunit structure

Interacts with MPDZ and INADL. May interact with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2. Interacts with GRM2 and DRD2; this may affect signaling By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell projectiondendrite By similarity. Cell projectionaxon By similarity. Cytoplasmic vesicle By similarity. Membranecaveola By similarity. Note: Localizes to the postsynaptic thickening of axo-dendritic synapses By similarity. Ref.2

Tissue specificity

Ubiquitous. Ref.2

Domain

The PDZ domain-binding motif is involved in the interaction with INADL, CASK, APBA1, DLG1 and DLG4 By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Biological processBehavior
   Cellular componentCell membrane
Cell projection
Cytoplasmic vesicle
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbehavior

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of behavior

Inferred from electronic annotation. Source: InterPro

regulation of hormone secretion

Inferred from electronic annotation. Source: InterPro

release of sequestered calcium ion into cytosol

Inferred from direct assay Ref.2. Source: UniProtKB

response to drug

Inferred from direct assay Ref.2. Source: UniProtKB

serotonin receptor signaling pathway

Inferred from direct assay Ref.2. Source: GOC

smooth muscle contraction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentaxon

Inferred from electronic annotation. Source: UniProtKB-SubCell

caveola

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasmic membrane-bounded vesicle

Inferred from electronic annotation. Source: UniProtKB-SubCell

dendritic shaft

Inferred from electronic annotation. Source: InterPro

integral component of plasma membrane

Inferred from electronic annotation. Source: InterPro

plasma membrane

Inferred from direct assay Ref.2. Source: UniProtKB

   Molecular_functiondrug binding

Inferred from direct assay Ref.2. Source: UniProtKB

serotonin binding

Inferred from direct assay Ref.2. Source: UniProtKB

serotonin receptor activity

Inferred from direct assay Ref.2. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4704705-hydroxytryptamine receptor 2A
PRO_0000068943

Regions

Topological domain1 – 7575Extracellular By similarity
Transmembrane76 – 9924Helical; Name=1; By similarity
Topological domain100 – 11011Cytoplasmic By similarity
Transmembrane111 – 13222Helical; Name=2; By similarity
Topological domain133 – 14816Extracellular By similarity
Transmembrane149 – 17123Helical; Name=3; By similarity
Topological domain172 – 19120Cytoplasmic By similarity
Transmembrane192 – 21524Helical; Name=4; By similarity
Topological domain216 – 23318Extracellular By similarity
Transmembrane234 – 25421Helical; Name=5; By similarity
Topological domain255 – 32369Cytoplasmic By similarity
Transmembrane324 – 34522Helical; Name=6; By similarity
Topological domain346 – 36116Extracellular By similarity
Transmembrane362 – 38322Helical; Name=7; By similarity
Topological domain384 – 47087Cytoplasmic By similarity
Region155 – 1606Agonist binding By similarity
Region335 – 3395Agonist binding By similarity
Motif172 – 1743DRY motif; important for ligand-induced conformation changes By similarity
Motif375 – 3795NPxxY motif; important for ligand-induced conformation changes and signaling By similarity
Motif468 – 4703PDZ-binding

Amino acid modifications

Glycosylation381N-linked (GlcNAc...) Potential
Glycosylation441N-linked (GlcNAc...) Potential
Glycosylation511N-linked (GlcNAc...) Potential
Glycosylation541N-linked (GlcNAc...) Potential
Disulfide bond148 ↔ 227 By similarity
Disulfide bond348 ↔ 352 By similarity

Sequences

Sequence LengthMass (Da)Tools
O46635 [UniParc].

Last modified April 12, 2005. Version 2.
Checksum: 5C654F81E88B92E3

FASTA47052,378
        10         20         30         40         50         60 
MDVLFEDNAP LSPTTSSLMP SNGDPRLYGN DLNAGDANTS DAFNWTVDAE NRTNLSCEGC 

        70         80         90        100        110        120 
LSPPCFSLLH LQEKNWSALL TAVVIILTIA GNILVIMAVS LEKKLQNATN YFLMSLAIAD 

       130        140        150        160        170        180 
MLLGFLVMPV SMLTILYGYR WPLPSKLCAV WIYLDVLFST ASIMHLCAIS LDRYVAIQNP 

       190        200        210        220        230        240 
IHHSRFNSRT KAFLKIIAVW TISVGISMPI PVFGLQDDSK VFKEGSCLLA DDNFVLIGSF 

       250        260        270        280        290        300 
VSFFIPLTIM VITYFLTIKS LQKEATLCVS DPGTRAKLAS FSFLPQSSLS SEKLFQRSIH 

       310        320        330        340        350        360 
REPGSYGRRT MQSISNEQKA CKVLGIVFFL FVVMWCPFFI TNIMAVICKE SCNEDIIGAL 

       370        380        390        400        410        420 
LNVFVWIGYL SSAVNPLVYT LFNKTYRSAF SRYIQCQYKE NKKPLQLILV NTIPALAYKS 

       430        440        450        460        470 
SQLQMGQKKN SKKDAKSTDN DYSMVALGKQ HSEDAPTDNI NTVNEKVSCV 

« Hide

References

[1]"Sequencing of canine 5-hydroxytriptamine receptor (5-HTR) 1B, 2A, 2C genes and identification of polymorphisms in the 5-HTR1B gene."
Masuda K., Hashizume C., Ogata N., Kikusui T., Takeuchi Y., Mori Y.
J. Vet. Med. Sci. 66:965-972(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Beagle.
[2]"Molecular and pharmacological characterization of serotonin 5-HT(2A) and 5-HT(2B) receptor subtypes in dog."
Bonaventure P., Nepomuceno D., Miller K., Chen J., Kuei C., Kamme F., Tran D.T., Lovenberg T.W., Liu C.
Eur. J. Pharmacol. 513:181-192(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]Sgard F.
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 120-307.
Tissue: Femoral artery.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB193092 mRNA. Translation: BAD60922.1.
AY832858 mRNA. Translation: AAX39385.1.
Y16134 mRNA. Translation: CAA76080.1.
RefSeqNP_001005869.1. NM_001005869.1.
UniGeneCfa.8880.

3D structure databases

ProteinModelPortalO46635.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBO46635.
ChEMBLCHEMBL5781.

Protein family/group databases

GPCRDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID403882.
KEGGcfa:403882.

Organism-specific databases

CTD3356.

Phylogenomic databases

eggNOGNOG247243.
HOGENOMHOG000240378.
HOVERGENHBG107487.
InParanoidO46635.
KOK04157.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000455. 5HT2A_rcpt.
IPR002231. 5HT_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24247:SF30. PTHR24247:SF30. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00516. 5HT2ARECEPTR.
PR01101. 5HTRECEPTOR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20817374.

Entry information

Entry name5HT2A_CANFA
AccessionPrimary (citable) accession number: O46635
Secondary accession number(s): Q50DZ9, Q60F96
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: April 12, 2005
Last modified: April 16, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries