Reviewed, UniProtKB/Swiss-Prot O46629 (ECHB_BOVIN)

Names and origin

Protein names

Recommended name:
    Trifunctional enzyme subunit beta, mitochondrial
Alternative name(s):
    TP-beta
Including the following 1 domains:
    1- Recommended name:
            3-ketoacyl-CoA thiolase
              EC=2.3.1.16
        Alternative name(s):
            Acetyl-CoA acyltransferase
            Beta-ketothiolase

Gene names

Name:

HADHB

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.
Pathway	Lipid metabolism; fatty acid beta-oxidation.
Subunit structure	Octamer of 4 alpha (HADHA) and 4 beta (HADHB) subunits By similarity.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA C-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Mitochondrion Potential
	Chain	35 – 475	441	Trifunctional enzyme subunit beta, mitochondrial		PRO_0000034079
Sites
	Active site	139	1	Acyl-thioester intermediate By similarity
	Active site	429	1	Proton acceptor By similarity
	Active site	459	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	73	1	N6-acetyllysine By similarity
	Modified residue	189	1	N6-acetyllysine By similarity
	Modified residue	202	1	N6-acetyllysine By similarity
	Modified residue	349	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O46629-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 5B55321E0337E197	FASTA	475	51,345
	10 20 30 40 50 60 MISLLTYTLK NLPNTSKWAL RFCMRPLSSS SQLQAAAASQ TKSKKTLAKP NIRNIVVVDG 70 80 90 100 110 120 VRTPFLLSGT SYKDLMPHDL ARAALSGLLH RTSVPKDVVD YIIFGTVIQE VKTSNVAREA 130 140 150 160 170 180 ALGAGFSDKT PAHTVTMACI SSNQAMTTAV GLIASGQCDV VVAGGVELMS DIPIRHSRKM 190 200 210 220 230 240 RKMMLDLNKA KTLAQRLSII SKFRLNFLSP ELPAVSEFST SETMGHSADR LAAAFAISRE 250 260 270 280 290 300 EQDEYALRSH SLAKKAQDEG LLSDVVPFKV PGRDTVTQDN GIRPSSLDQM AKLKPAFIKP 310 320 330 340 350 360 YGTVTAANSS FLTDGASAVL IMAEEKALAM GYKPKAYLRD FMYVSQDPKD QLLLGPTYAT 370 380 390 400 410 420 PKVLEKAGLT MNDIDVFEFH EAFSGQILAN LKAMDSDWFA QNYMGRKAKV GLPPLEKFNN 430 440 450 460 470 WGGSLSLGHP FGATGCRLVM AAANRLRKEG GQYGLVAACA AGGQGHAMIV EAYPK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Cailleret K., Chevet E., Lemaitre G., Dahan S., Bergeron J.J., Katinka M.D. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ003066 mRNA. Translation: CAA05840.1. BC102638 mRNA. Translation: AAI02639.1.
IPI	IPI00697915.
RefSeq	NP_776761.1.
UniGene	Bt.52915
3D structure databases
HSSP	HSSP built from PDB template 1AFW based on UniProtKB P27796.
ModBase	Search...
Protein-protein interaction databases
STRING	O46629.
Genome annotation databases
Ensembl	ENSBTAT00000013310; ENSBTAP00000013310; ENSBTAG00000010083; Bos taurus. [Genome view]
GeneID	281811.
KEGG	bta:281811.
Organism-specific databases
CTD	281811.
Phylogenomic databases
HOVERGEN	O46629.
OMA	AMDSDWF.
Enzyme and pathway databases
BRENDA	2.3.1.16. 251.
Family and domain databases
InterPro	IPR002155. Thiolase. [Graphical view]
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ECHB_BOVIN

Accession

Primary (citable) accession number: O46629
Secondary accession number(s): Q3SZZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)