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Protein

Prolactin receptor

Gene

PRLR

Organism
Ovis aries (Sheep)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This is a receptor for the anterior pituitary hormone prolactin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi211 – 2111ZincBy similarity
Metal bindingi212 – 2121ZincBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Prolactin receptor
Short name:
PRL-R
Short name:
oPR
Gene namesi
Name:PRLR
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
Proteomesi
  • UP000002356 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 237213ExtracellularSequence analysisAdd
BLAST
Transmembranei238 – 25821HelicalSequence analysisAdd
BLAST
Topological domaini259 – 581323CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 581557Prolactin receptorPRO_0000010982Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi36 ↔ 46By similarity
Glycosylationi59 – 591N-linked (GlcNAc...)Sequence analysis
Disulfide bondi75 ↔ 86By similarity
Glycosylationi132 – 1321N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed in all tissues examined; liver, pituitary, adrenal gland, ovary and fetal liver.2 Publications

Interactioni

Subunit structurei

Interacts with SMARCA1. Interacts with NEK3 and VAV2 and this interaction is prolactin-dependent.By similarity

Structurei

3D structure databases

ProteinModelPortaliO46561.
SMRiO46561. Positions 30-227.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 127101Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini129 – 229101Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi215 – 2195WSXWS motif
Motifi267 – 2759Box 1 motif

Domaini

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.

Sequence similaritiesi

Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG007314.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR015152. Growth/epo_recpt_lig-bind.
IPR013783. Ig-like_fold.
IPR003528. Long_hematopoietin_rcpt_CS.
IPR033230. PRLR.
[Graphical view]
PANTHERiPTHR23036:SF86. PTHR23036:SF86. 1 hit.
PfamiPF09067. EpoR_lig-bind. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 2 hits.
PS01352. HEMATOPO_REC_L_F1. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O46561-1) [UniParc]FASTAAdd to basket

Also known as: Long, L-OPR

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKENAASRVL FILLLFLFAS LLNGQSPPEK PKLIKCRSPG KETFTCWWEP
60 70 80 90 100
GADGGLPTNY TLTYRKEGET LIHECPDYKT GGPNSCYFSK KYTSIWKMYV
110 120 130 140 150
ITVSAINQMG ISSSDPLYVD VTYIVEPEPP VNLTLELKHP EDRKPYLWIK
160 170 180 190 200
WSPPTLTDVK SGWFSIQYEI RLKPEKATDW ETHFAPKLTQ LKIFNLYPGQ
210 220 230 240 250
KYLVQIRCKP DHGYWSEWSP ESFIQIPNDF PVKDTSMWIF VGVLSAVICL
260 270 280 290 300
IMVWAVALKG YSMVTCILPP VPGPKIKGFD IHLLEKGKSE ELLRALESQD
310 320 330 340 350
FLPTSDCEDL LMEFIEVDDS EDQHLMPHPS KEHMEQGVKP MHLDPDTDSG
360 370 380 390 400
RGSCDSPSLL SEKCDEPQAY PSKFHIPEGP EKLEDPETNH TCLQAPQSTS
410 420 430 440 450
GEGKIPYFLA NGPKSSTWPF PQPPSLYSPR YSYHNIADVC ELALGMAGTT
460 470 480 490 500
ATLLDQTDQH AFKPSKTIET GGEGKAAKQS ESEGYSSEPD QDMAWPLLQD
510 520 530 540 550
KTPLFSAKPL EYVEIHKVSQ DGVLALFPKQ NEKVDAPETS KEYSKVSRVT
560 570 580
DSNILVLIPD LQAQNLTLLE ESAKKAPPAL P
Length:581
Mass (Da):65,235
Last modified:June 1, 1998 - v1
Checksum:iEC534FDE538837A0
GO
Isoform 2 (identifier: O46561-2) [UniParc]FASTAAdd to basket

Also known as: Short, S-OPR

The sequence of this isoform differs from the canonical sequence as follows:
     286-296: KGKSEELLRAL → ISQPSRLVSVF
     297-581: Missing.

Show »
Length:296
Mass (Da):33,847
Checksum:iF0AE544C76401507
GO
Isoform 3 (identifier: O46561-3) [UniParc]FASTAAdd to basket

Also known as: Soluble

The sequence of this isoform differs from the canonical sequence as follows:
     24-66: GQSPPEKPKL...PTNYTLTYRK → ASLYVPGGKC...DQYLLLTVTS
     67-581: Missing.

Show »
Length:66
Mass (Da):7,319
Checksum:iAD5DC3A86779B45C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti281 – 2811I → V in AAB97743 (PubMed:9343303).Curated
Sequence conflicti281 – 2811I → V in AAB97744 (PubMed:9343303).Curated
Sequence conflicti387 – 3871E → K in CAA71597 (PubMed:9832462).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei24 – 6643GQSPP…LTYRK → ASLYVPGGKCSSVCTYMAYP FVGGIFLHMYLCVDQYLLLT VTS in isoform 3. CuratedVSP_001730Add
BLAST
Alternative sequencei67 – 581515Missing in isoform 3. CuratedVSP_001731Add
BLAST
Alternative sequencei286 – 29611KGKSEELLRAL → ISQPSRLVSVF in isoform 2. CuratedVSP_001732Add
BLAST
Alternative sequencei297 – 581285Missing in isoform 2. CuratedVSP_001733Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041257 mRNA. Translation: AAB96795.1.
AF041977 mRNA. Translation: AAB96920.1.
AF041979 mRNA. Translation: AAB97082.1.
AF042358 Genomic DNA. Translation: AAB97744.1.
AF042358 Genomic DNA. Translation: AAB97743.1.
AF041978 mRNA. Translation: AAB96965.1.
Y10578 mRNA. Translation: CAA71597.1.
Y10808 mRNA. Translation: CAA71766.1.
RefSeqiNP_001009204.1. NM_001009204.1. [O46561-3]
UniGeneiOar.4812.
Oar.576.

Genome annotation databases

GeneIDi443020.
KEGGioas:443020.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF041257 mRNA. Translation: AAB96795.1.
AF041977 mRNA. Translation: AAB96920.1.
AF041979 mRNA. Translation: AAB97082.1.
AF042358 Genomic DNA. Translation: AAB97744.1.
AF042358 Genomic DNA. Translation: AAB97743.1.
AF041978 mRNA. Translation: AAB96965.1.
Y10578 mRNA. Translation: CAA71597.1.
Y10808 mRNA. Translation: CAA71766.1.
RefSeqiNP_001009204.1. NM_001009204.1. [O46561-3]
UniGeneiOar.4812.
Oar.576.

3D structure databases

ProteinModelPortaliO46561.
SMRiO46561. Positions 30-227.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi443020.
KEGGioas:443020.

Organism-specific databases

CTDi19066.

Phylogenomic databases

HOVERGENiHBG007314.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR015152. Growth/epo_recpt_lig-bind.
IPR013783. Ig-like_fold.
IPR003528. Long_hematopoietin_rcpt_CS.
IPR033230. PRLR.
[Graphical view]
PANTHERiPTHR23036:SF86. PTHR23036:SF86. 1 hit.
PfamiPF09067. EpoR_lig-bind. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 2 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 2 hits.
PS01352. HEMATOPO_REC_L_F1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Long and short forms of the ovine prolactin receptor: cDNA cloning and genomic analysis reveal that the two forms arise by different alternative splicing mechanisms in ruminants and in rodents."
    Bignon C., Binart N., Ormandy C., Schuler L.A., Kelly P.A., Djiane J.
    J. Mol. Endocrinol. 19:109-120(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING.
    Tissue: Liver and Mammary gland.
  2. "Detection of prolactin receptor gene expression in the sheep pituitary gland and visualization of the specific translation of the signal in gonadotrophs."
    Tortonese D.J., Brooks J., Ingleton P.M., McNeilly A.S.
    Endocrinology 139:5215-5223(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 61-395, ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
    Strain: Scottish blackface.
    Tissue: Pituitary anterior lobe.
  3. "Two forms of the prolactin receptor messenger ribonucleic acid are present in ovine fetal liver and adult ovary."
    Anthony R.V., Smith G.W., Duong A., Pratt S.L., Smith M.F.
    Endocrine 3:291-295(1995)
    Cited for: NUCLEOTIDE SEQUENCE OF 147-302, ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
    Tissue: Corpus luteum and Fetal liver.

Entry informationi

Entry nameiPRLR_SHEEP
AccessioniPrimary (citable) accession number: O46561
Secondary accession number(s): O46569
, O46573, O46574, P79203, P79205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: June 1, 1998
Last modified: May 11, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.