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Protein

Cytochrome b-245 heavy chain

Gene

CYBB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes.

Cofactori

FADCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi101Iron (heme axial ligand)Curated1
Metal bindingi115Iron (heme axial ligand)Curated1
Metal bindingi209Iron (heme axial ligand)Curated1
Metal bindingi222Iron (heme axial ligand)Curated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi338 – 344FADSequence analysis7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Oxidoreductase, Voltage-gated channel
Biological processElectron transport, Ion transport, Transport
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-BTA-1222556. ROS, RNS production in phagocytes.
R-BTA-1236973. Cross-presentation of particulate exogenous antigens (phagosomes).
R-BTA-3299685. Detoxification of Reactive Oxygen Species.
R-BTA-4420097. VEGFA-VEGFR2 Pathway.
R-BTA-5668599. RHO GTPases Activate NADPH Oxidases.
R-BTA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-245 heavy chain (EC:1.-.-.-)
Alternative name(s):
CGD91-phox
Cytochrome b(558) subunit beta
Short name:
Cytochrome b558 subunit beta
Heme-binding membrane glycoprotein gp91phox
Neutrophil cytochrome b 91 kDa polypeptide
gp91-1
gp91-phox
p22 phagocyte B-cytochrome
Gene namesi
Name:CYBB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome X

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Topological domaini30 – 48ExtracellularSequence analysisAdd BLAST19
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Topological domaini70 – 102CytoplasmicSequence analysisAdd BLAST33
Transmembranei103 – 123HelicalSequence analysisAdd BLAST21
Topological domaini124 – 169ExtracellularSequence analysisAdd BLAST46
Transmembranei170 – 190HelicalSequence analysisAdd BLAST21
Topological domaini191 – 200CytoplasmicSequence analysis10
Transmembranei201 – 221HelicalSequence analysisAdd BLAST21
Topological domaini222 – 261ExtracellularSequence analysisAdd BLAST40
Transmembranei262 – 282HelicalSequence analysisAdd BLAST21
Topological domaini283 – 570CytoplasmicSequence analysisAdd BLAST288

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002101441 – 570Cytochrome b-245 heavy chainAdd BLAST570

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi132N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi149N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi247N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Phosphorylated on Ser and Thr residues.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO46522.
PRIDEiO46522.

Expressioni

Gene expression databases

BgeeiENSBTAG00000019953.

Interactioni

Subunit structurei

Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1. Interacts with calprotectin (S100A8/9). Interacts with NRROS; the interaction is direct and impairs formation of a stable NADPH oxidase complex (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026580.

Structurei

3D structure databases

ProteinModelPortaliO46522.
SMRiO46522.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 286Ferric oxidoreductaseAdd BLAST233
Domaini287 – 397FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST111

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
GeneTreeiENSGT00550000074350.
HOGENOMiHOG000216669.
HOVERGENiHBG003760.
InParanoidiO46522.
KOiK21421.
OMAiKAGQWLF.
OrthoDBiEOG091G09RV.
TreeFamiTF105354.

Family and domain databases

InterProiView protein in InterPro
IPR000778. Cyt_b245_heavy_chain.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
PfamiView protein in Pfam
PF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiView protein in PROSITE
PS51384. FAD_FR. 1 hit.

Sequencei

Sequence statusi: Complete.

O46522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNWVVNEGI SIFVILVWLG MNVFLFVWYY RVYDIPDKFF YTRKLLGSAL
60 70 80 90 100
ALARAPAACL NFNCMLILLP VCRNLLSFLR GSSACCSTRI RRQLDRNLTF
110 120 130 140 150
HKMVAWMIAL HTAIHTIAHL FNVEWCVNAR VNNSDPYSIA LSDIGDKPNE
160 170 180 190 200
TYLNFVRQRI KNPEGGLYVA VTRLAGITGV VITLCLILII TSSTKTIRRS
210 220 230 240 250
YFEVFWYTHH LFVIFFIGLA IHGAQRIVRG QTAESLLKHQ PRNCYQNISQ
260 270 280 290 300
WGKIENCPIP EFSGNPPMTW KWIVGPMFLY LCERLVRFWR SQQKVVITKV
310 320 330 340 350
VTHPFKTIEL QMKKKGFKME VGQYIFVKCP VVSKLEWHPF TLTSAPEEDF
360 370 380 390 400
FSIHIRIVGD WTEGLFKACG CDKQEFQDAW KLPKIAVDGP FGTASEDVFS
410 420 430 440 450
YEVVMLVGAG IGVTPFASIL KSVWYKYCNK APNLRLKKIY FYWLCRDTHA
460 470 480 490 500
FEWFADLLQL LETQMQEKNN TDFLSYNICL TGWDESQASH FAMHHDEEKD
510 520 530 540 550
VITGLKQKTL YGRPNWDNEF KTIGSQHPNT RIGVFLCGPE ALADTLNKQC
560 570
ISNSDSGPRG VHFIFNKENF
Length:570
Mass (Da):65,643
Last modified:June 1, 1998 - v1
Checksum:i959236166089BCCC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036097 mRNA. Translation: AAC27247.1.
BC134469 mRNA. Translation: AAI34470.1.
RefSeqiNP_776460.1. NM_174035.4.
UniGeneiBt.4558.

Genome annotation databases

EnsembliENSBTAT00000026580; ENSBTAP00000026580; ENSBTAG00000019953.
GeneIDi281112.
KEGGibta:281112.

Similar proteinsi

Entry informationi

Entry nameiCY24B_BOVIN
AccessioniPrimary (citable) accession number: O46522
Secondary accession number(s): A4IF96
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: October 25, 2017
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome