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Protein

Cytochrome b-245 heavy chain

Gene

CYBB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes.

Cofactori

FADCurated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi101 – 1011Iron (heme axial ligand)Curated
Metal bindingi115 – 1151Iron (heme axial ligand)Curated
Metal bindingi209 – 2091Iron (heme axial ligand)Curated
Metal bindingi222 – 2221Iron (heme axial ligand)Curated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi338 – 3447FADSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Oxidoreductase, Voltage-gated channel

Keywords - Biological processi

Electron transport, Ion transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-BTA-1222556. ROS, RNS production in response to bacteria.
R-BTA-1236973. Cross-presentation of particulate exogenous antigens (phagosomes).
R-BTA-3299685. Detoxification of Reactive Oxygen Species.
R-BTA-4420097. VEGFA-VEGFR2 Pathway.
R-BTA-5668599. RHO GTPases Activate NADPH Oxidases.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-245 heavy chain (EC:1.-.-.-)
Alternative name(s):
CGD91-phox
Cytochrome b(558) subunit beta
Short name:
Cytochrome b558 subunit beta
Heme-binding membrane glycoprotein gp91phox
Neutrophil cytochrome b 91 kDa polypeptide
gp91-1
gp91-phox
p22 phagocyte B-cytochrome
Gene namesi
Name:CYBB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome X

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 87CytoplasmicSequence analysis
Transmembranei9 – 2921HelicalSequence analysisAdd
BLAST
Topological domaini30 – 4819ExtracellularSequence analysisAdd
BLAST
Transmembranei49 – 6921HelicalSequence analysisAdd
BLAST
Topological domaini70 – 10233CytoplasmicSequence analysisAdd
BLAST
Transmembranei103 – 12321HelicalSequence analysisAdd
BLAST
Topological domaini124 – 16946ExtracellularSequence analysisAdd
BLAST
Transmembranei170 – 19021HelicalSequence analysisAdd
BLAST
Topological domaini191 – 20010CytoplasmicSequence analysis
Transmembranei201 – 22121HelicalSequence analysisAdd
BLAST
Topological domaini222 – 26140ExtracellularSequence analysisAdd
BLAST
Transmembranei262 – 28221HelicalSequence analysisAdd
BLAST
Topological domaini283 – 570288CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • lysosome Source: AgBase
  • membrane raft Source: AgBase
  • mitochondrion Source: Ensembl
  • NADPH oxidase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 570569Cytochrome b-245 heavy chainPRO_0000210144Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi132 – 1321N-linked (GlcNAc...)Sequence analysis
Glycosylationi149 – 1491N-linked (GlcNAc...)Sequence analysis
Glycosylationi247 – 2471N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Phosphorylated on Ser and Thr residues.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO46522.
PRIDEiO46522.

Expressioni

Gene expression databases

BgeeiENSBTAG00000019953.

Interactioni

Subunit structurei

Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1. Interacts with calprotectin (S100A8/9). Interacts with NRROS; the interaction is direct and impairs formation of a stable NADPH oxidase complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026580.

Structurei

3D structure databases

ProteinModelPortaliO46522.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 286233Ferric oxidoreductaseAdd
BLAST
Domaini287 – 397111FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
GeneTreeiENSGT00550000074350.
HOGENOMiHOG000216669.
HOVERGENiHBG003760.
InParanoidiO46522.
KOiK08008.
OMAiEPQEAWK.
OrthoDBiEOG091G09RV.
TreeFamiTF105354.

Family and domain databases

InterProiIPR000778. Cyt_b245_heavy_chain.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O46522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNWVVNEGI SIFVILVWLG MNVFLFVWYY RVYDIPDKFF YTRKLLGSAL
60 70 80 90 100
ALARAPAACL NFNCMLILLP VCRNLLSFLR GSSACCSTRI RRQLDRNLTF
110 120 130 140 150
HKMVAWMIAL HTAIHTIAHL FNVEWCVNAR VNNSDPYSIA LSDIGDKPNE
160 170 180 190 200
TYLNFVRQRI KNPEGGLYVA VTRLAGITGV VITLCLILII TSSTKTIRRS
210 220 230 240 250
YFEVFWYTHH LFVIFFIGLA IHGAQRIVRG QTAESLLKHQ PRNCYQNISQ
260 270 280 290 300
WGKIENCPIP EFSGNPPMTW KWIVGPMFLY LCERLVRFWR SQQKVVITKV
310 320 330 340 350
VTHPFKTIEL QMKKKGFKME VGQYIFVKCP VVSKLEWHPF TLTSAPEEDF
360 370 380 390 400
FSIHIRIVGD WTEGLFKACG CDKQEFQDAW KLPKIAVDGP FGTASEDVFS
410 420 430 440 450
YEVVMLVGAG IGVTPFASIL KSVWYKYCNK APNLRLKKIY FYWLCRDTHA
460 470 480 490 500
FEWFADLLQL LETQMQEKNN TDFLSYNICL TGWDESQASH FAMHHDEEKD
510 520 530 540 550
VITGLKQKTL YGRPNWDNEF KTIGSQHPNT RIGVFLCGPE ALADTLNKQC
560 570
ISNSDSGPRG VHFIFNKENF
Length:570
Mass (Da):65,643
Last modified:June 1, 1998 - v1
Checksum:i959236166089BCCC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036097 mRNA. Translation: AAC27247.1.
BC134469 mRNA. Translation: AAI34470.1.
RefSeqiNP_776460.1. NM_174035.4.
UniGeneiBt.4558.

Genome annotation databases

EnsembliENSBTAT00000026580; ENSBTAP00000026580; ENSBTAG00000019953.
GeneIDi281112.
KEGGibta:281112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036097 mRNA. Translation: AAC27247.1.
BC134469 mRNA. Translation: AAI34470.1.
RefSeqiNP_776460.1. NM_174035.4.
UniGeneiBt.4558.

3D structure databases

ProteinModelPortaliO46522.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026580.

Proteomic databases

PaxDbiO46522.
PRIDEiO46522.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000026580; ENSBTAP00000026580; ENSBTAG00000019953.
GeneIDi281112.
KEGGibta:281112.

Organism-specific databases

CTDi1536.

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
GeneTreeiENSGT00550000074350.
HOGENOMiHOG000216669.
HOVERGENiHBG003760.
InParanoidiO46522.
KOiK08008.
OMAiEPQEAWK.
OrthoDBiEOG091G09RV.
TreeFamiTF105354.

Enzyme and pathway databases

ReactomeiR-BTA-1222556. ROS, RNS production in response to bacteria.
R-BTA-1236973. Cross-presentation of particulate exogenous antigens (phagosomes).
R-BTA-3299685. Detoxification of Reactive Oxygen Species.
R-BTA-4420097. VEGFA-VEGFR2 Pathway.
R-BTA-5668599. RHO GTPases Activate NADPH Oxidases.

Gene expression databases

BgeeiENSBTAG00000019953.

Family and domain databases

InterProiIPR000778. Cyt_b245_heavy_chain.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCY24B_BOVIN
AccessioniPrimary (citable) accession number: O46522
Secondary accession number(s): A4IF96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.