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Protein

Cytochrome b-245 light chain

Gene

CYBA

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi94Iron (heme axial ligand)Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-BTA-1222556. ROS, RNS production in phagocytes.
R-BTA-1236973. Cross-presentation of particulate exogenous antigens (phagosomes).
R-BTA-3299685. Detoxification of Reactive Oxygen Species.
R-BTA-4420097. VEGFA-VEGFR2 Pathway.
R-BTA-5668599. RHO GTPases Activate NADPH Oxidases.
R-BTA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-245 light chain
Alternative name(s):
Cytochrome b(558) alpha chain
Cytochrome b558 subunit alpha
Neutrophil cytochrome b 22 kDa polypeptide
Superoxide-generating NADPH oxidase light chain subunit
p22 phagocyte B-cytochrome
p22-phox
Short name:
p22phox
Gene namesi
Name:CYBA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 18

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Intramembranei91 – 127By similarityAdd BLAST37

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2366457.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001449061 – 191Cytochrome b-245 light chainAdd BLAST191

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei147PhosphothreonineBy similarity1
Modified residuei168PhosphoserineBy similarity1

Post-translational modificationi

The heme prosthetic group could be coordinated with residues of the light chain, the heavy chain, or both, and it is possible that more than one heme is present per cytochrome b-245.
Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting p47phox binding.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO46521.
PeptideAtlasiO46521.
PRIDEiO46521.

Expressioni

Gene expression databases

BgeeiENSBTAG00000003895.

Interactioni

Subunit structurei

Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1 (via SH3 domain). Interacts with SH3PXD2A (By similarity). Interacts with DUOX1, DUOX2 and TPO. Interacts with NOX3 and NOX4. Interacts with calprotectin (S100A8/9) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000005080.

Structurei

3D structure databases

SMRiO46521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the p22phox family.Curated

Phylogenomic databases

eggNOGiENOG410IXYN. Eukaryota.
ENOG4111MI6. LUCA.
GeneTreeiENSGT00390000002290.
HOGENOMiHOG000001585.
HOVERGENiHBG051278.
InParanoidiO46521.
KOiK08009.
OMAiEQWTPIE.
OrthoDBiEOG091G0NNC.
TreeFamiTF328901.

Family and domain databases

InterProiView protein in InterPro
IPR007732. Cyt_b558_asu.
PANTHERiPTHR15168. PTHR15168. 1 hit.
PfamiView protein in Pfam
PF05038. Cytochrom_B558a. 1 hit.
PIRSFiPIRSF019635. Cytochr_b558a. 1 hit.

Sequencei

Sequence statusi: Complete.

O46521-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQIEWAMWA NEQALASGLI LITGGIVATA GQFTQWYLGA YSIAAGVLVC
60 70 80 90 100
LLEYPRGKRS KGSTMERCGQ KYLTRVVKLF GPLTRNYYIR AFLHLGLAVP
110 120 130 140 150
AGFLLATILG TACLAIASGI YLLAAIRGEQ WSPIEPKPKE RPQIGGTIKQ
160 170 180 190
PPSNPPPRPP AEARKKPSEE AAGVPTGGPQ ENPMPVNDEV V
Length:191
Mass (Da):20,496
Last modified:January 23, 2007 - v3
Checksum:iCE1614D50C1FBAC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036096 mRNA. Translation: AAC27246.1.
BC142380 mRNA. Translation: AAI42381.1.
RefSeqiNP_776459.1. NM_174034.2.
UniGeneiBt.4140.

Genome annotation databases

EnsembliENSBTAT00000005080; ENSBTAP00000005080; ENSBTAG00000003895.
GeneIDi281111.
KEGGibta:281111.

Similar proteinsi

Entry informationi

Entry nameiCY24A_BOVIN
AccessioniPrimary (citable) accession number: O46521
Secondary accession number(s): A5PK68
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 114 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families