ID   CP11A_HORSE             Reviewed;         520 AA.
AC   O46515;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   16-JUN-2009, entry version 58.
DE   RecName: Full=Cholesterol side-chain cleavage enzyme, mitochondrial;
DE            EC=1.14.15.6;
DE   AltName: Full=Cytochrome P450 11A1;
DE   AltName: Full=CYPXIA1;
DE   AltName: Full=P450(scc);
DE   AltName: Full=Cholesterol desmolase;
DE   Flags: Precursor;
GN   Name=CYP11A1;
OS   Equus caballus (Horse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX   NCBI_TaxID=9796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Ovarian follicle membrane;
RX   MEDLINE=20575290; PubMed=11133676;
RA   Boerboom D., Sirois J.;
RT   "Equine P450 cholesterol side-chain cleavage and 3 beta-hydroxysteroid
RT   dehydrogenase/delta(5)-delta(4) isomerase: molecular cloning and
RT   regulation of their messenger ribonucleic acids in equine follicles
RT   during the ovulatory process.";
RL   Biol. Reprod. 64:206-215(2001).
CC   -!- FUNCTION: Catalyzes the side-chain cleavage reaction of
CC       cholesterol to pregnenolone.
CC   -!- CATALYTIC ACTIVITY: Cholesterol + reduced adrenal ferredoxin +
CC       O(2) = pregnenolone + 4-methylpentanal + oxidized adrenal
CC       ferredoxin + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Lipid metabolism; C21-steroid hormone metabolism.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; AF031664; AAC04700.1; -; Genomic_DNA.
DR   RefSeq; NP_001075990.1; -.
DR   UniGene; Eca.13386; -.
DR   HSSP; P00189; 1SCC.
DR   Ensembl; ENSECAG00000017494; Equus caballus.
DR   GeneID; 100034229; -.
DR   KEGG; ecb:100034229; -.
DR   HOVERGEN; O46515; -.
DR   BRENDA; 1.14.15.6; 1464.
DR   GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008386; F:cholesterol monooxygenase (side-chain-cleav...; IEA:EC.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0006700; P:C21-steroid hormone biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   3: Inferred from homology;
KW   Cholesterol metabolism; Heme; Iron; Lipid metabolism; Membrane;
KW   Metal-binding; Mitochondrion; Monooxygenase; Oxidoreductase;
KW   Steroid metabolism; Steroidogenesis; Transit peptide.
FT   TRANSIT       1     39       Mitochondrion (By similarity).
FT   CHAIN        40    520       Cholesterol side-chain cleavage enzyme,
FT                                mitochondrial.
FT                                /FTId=PRO_0000003584.
FT   METAL       461    461       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   520 AA;  60314 MW;  B8420BF587850192 CRC64;
     MLVRGLPLRS VLVKGCQPLL SAPREGPGHP RVPTGEGAGM SSHSPRPFKE IPSPGDNGWI
     NLYHFWREKG PKKLHYHHFQ NFQKYGPIYR EKLGNVESVY IVDPEDVALL FKFEGPHPER
     FLIPPWTAYH QYFQKPVGVL FKSSDAWKKD RLALNPEVMA LESIKNFIPL LDPVSQDFVS
     LLHRRMEQQG SGKFSGPIIE DLFRFAFESI TNVIFGERQG MLDEIVDPEA QRFIDAVYKM
     FHTSVPMLSL PPDLFRLFRT KTWRDHVAAW DTVFSKAEQY TEKFYQDLKQ KRHFDSYPGI
     FYRLLASNKL PFKDIQANVT EMLAGGVDTT SMSLQWHLYE IARNLRVQEM LREEVLAARR
     QAQGDTSTMV QMVPLLKASI KETLRLHPIA VTLQRYPQND LVIRDYMIPA KTLVQVSIYT
     MGQDPTFFSN PRRFDPTRWL DKNKDLTHFR NLGFGWGVRQ CLGRRIAELE MTLFLIHILE
     NFRVEIQHLN DVDSTFGLIL IPEKPISFTF WPITRAPPQA
//