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O46515

- CP11A_HORSE

UniProt

O46515 - CP11A_HORSE

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Protein

Cholesterol side-chain cleavage enzyme, mitochondrial

Gene

CYP11A1

Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.

Catalytic activityi

Cholesterol + 6 reduced adrenodoxin + 3 O2 = pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O.

Cofactori

Heme group.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi461 – 4611Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. cholesterol monooxygenase (side-chain-cleaving) activity Source: UniProtKB
  2. heme binding Source: UniProtKB
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. C21-steroid hormone biosynthetic process Source: UniProtKB
  2. cholesterol metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Steroidogenesis, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00229.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol side-chain cleavage enzyme, mitochondrial (EC:1.14.15.6)
Alternative name(s):
CYPXIA1
Cholesterol desmolase
Cytochrome P450 11A1
Cytochrome P450(scc)
Gene namesi
Name:CYP11A1
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
ProteomesiUP000002281: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: UniProtKB-KW
  2. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3939MitochondrionBy similarityAdd
BLAST
Chaini40 – 520481Cholesterol side-chain cleavage enzyme, mitochondrialPRO_0000003584Add
BLAST

Proteomic databases

PRIDEiO46515.

Interactioni

Subunit structurei

Interacts with FDX1/adrenodoxin.By similarity

Protein-protein interaction databases

STRINGi9796.ENSECAP00000015487.

Structurei

3D structure databases

ProteinModelPortaliO46515.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000013161.
HOVERGENiHBG051098.
InParanoidiO46515.
KOiK00498.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O46515-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLVRGLPLRS VLVKGCQPLL SAPREGPGHP RVPTGEGAGM SSHSPRPFKE
60 70 80 90 100
IPSPGDNGWI NLYHFWREKG PKKLHYHHFQ NFQKYGPIYR EKLGNVESVY
110 120 130 140 150
IVDPEDVALL FKFEGPHPER FLIPPWTAYH QYFQKPVGVL FKSSDAWKKD
160 170 180 190 200
RLALNPEVMA LESIKNFIPL LDPVSQDFVS LLHRRMEQQG SGKFSGPIIE
210 220 230 240 250
DLFRFAFESI TNVIFGERQG MLDEIVDPEA QRFIDAVYKM FHTSVPMLSL
260 270 280 290 300
PPDLFRLFRT KTWRDHVAAW DTVFSKAEQY TEKFYQDLKQ KRHFDSYPGI
310 320 330 340 350
FYRLLASNKL PFKDIQANVT EMLAGGVDTT SMSLQWHLYE IARNLRVQEM
360 370 380 390 400
LREEVLAARR QAQGDTSTMV QMVPLLKASI KETLRLHPIA VTLQRYPQND
410 420 430 440 450
LVIRDYMIPA KTLVQVSIYT MGQDPTFFSN PRRFDPTRWL DKNKDLTHFR
460 470 480 490 500
NLGFGWGVRQ CLGRRIAELE MTLFLIHILE NFRVEIQHLN DVDSTFGLIL
510 520
IPEKPISFTF WPITRAPPQA
Length:520
Mass (Da):60,314
Last modified:June 1, 1998 - v1
Checksum:iB8420BF587850192
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF031664 Genomic DNA. Translation: AAC04700.1.
RefSeqiNP_001075990.1. NM_001082521.1.
UniGeneiEca.13386.

Genome annotation databases

GeneIDi100034229.
KEGGiecb:100034229.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF031664 Genomic DNA. Translation: AAC04700.1 .
RefSeqi NP_001075990.1. NM_001082521.1.
UniGenei Eca.13386.

3D structure databases

ProteinModelPortali O46515.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9796.ENSECAP00000015487.

Proteomic databases

PRIDEi O46515.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100034229.
KEGGi ecb:100034229.

Organism-specific databases

CTDi 1583.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000013161.
HOVERGENi HBG051098.
InParanoidi O46515.
KOi K00498.

Enzyme and pathway databases

UniPathwayi UPA00229 .

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Equine P450 cholesterol side-chain cleavage and 3 beta-hydroxysteroid dehydrogenase/delta(5)-delta(4) isomerase: molecular cloning and regulation of their messenger ribonucleic acids in equine follicles during the ovulatory process."
    Boerboom D., Sirois J.
    Biol. Reprod. 64:206-215(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Ovarian follicle membrane.

Entry informationi

Entry nameiCP11A_HORSE
AccessioniPrimary (citable) accession number: O46515
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3