O46515 (CP11A_HORSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cholesterol side-chain cleavage enzyme, mitochondrial
EC=1.14.15.6

Alternative name(s):
CYPXIA1
Cholesterol desmolase
Cytochrome P450 11A1
Cytochrome P450(scc)

Gene names

Name:

CYP11A1

Organism

Equus caballus (Horse) [Reference proteome]

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus › Equus

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.
Catalytic activity	Cholesterol + 6 reduced adrenodoxin + 3 O2 = pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; C21-steroid hormone metabolism.
Subunit structure	Interacts with FDX1/adrenodoxin By similarity.
Subcellular location	Mitochondrion membrane.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism Steroidogenesis Sterol metabolism
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	C21-steroid hormone metabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cholesterol monooxygenase (side-chain-cleaving) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Mitochondrion By similarity
	Chain	40 – 520	481	Cholesterol side-chain cleavage enzyme, mitochondrial		PRO_0000003584
Sites
	Metal binding	461	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O46515 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: B8420BF587850192	FASTA	520	60,314
	10 20 30 40 50 60 MLVRGLPLRS VLVKGCQPLL SAPREGPGHP RVPTGEGAGM SSHSPRPFKE IPSPGDNGWI 70 80 90 100 110 120 NLYHFWREKG PKKLHYHHFQ NFQKYGPIYR EKLGNVESVY IVDPEDVALL FKFEGPHPER 130 140 150 160 170 180 FLIPPWTAYH QYFQKPVGVL FKSSDAWKKD RLALNPEVMA LESIKNFIPL LDPVSQDFVS 190 200 210 220 230 240 LLHRRMEQQG SGKFSGPIIE DLFRFAFESI TNVIFGERQG MLDEIVDPEA QRFIDAVYKM 250 260 270 280 290 300 FHTSVPMLSL PPDLFRLFRT KTWRDHVAAW DTVFSKAEQY TEKFYQDLKQ KRHFDSYPGI 310 320 330 340 350 360 FYRLLASNKL PFKDIQANVT EMLAGGVDTT SMSLQWHLYE IARNLRVQEM LREEVLAARR 370 380 390 400 410 420 QAQGDTSTMV QMVPLLKASI KETLRLHPIA VTLQRYPQND LVIRDYMIPA KTLVQVSIYT 430 440 450 460 470 480 MGQDPTFFSN PRRFDPTRWL DKNKDLTHFR NLGFGWGVRQ CLGRRIAELE MTLFLIHILE 490 500 510 520 NFRVEIQHLN DVDSTFGLIL IPEKPISFTF WPITRAPPQA

References

[1]

"Equine P450 cholesterol side-chain cleavage and 3 beta-hydroxysteroid dehydrogenase/delta(5)-delta(4) isomerase: molecular cloning and regulation of their messenger ribonucleic acids in equine follicles during the ovulatory process." Boerboom D., Sirois J. Biol. Reprod. 64:206-215(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Ovarian follicle membrane.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF031664 Genomic DNA. Translation: AAC04700.1.
RefSeq	NP_001075990.1. NM_001082521.1.
UniGene	Eca.13386.
3D structure databases
ProteinModelPortal	O46515.
ModBase	Search...
Protein-protein interaction databases
STRING	9796.ENSECAP00000015487.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100034229.
KEGG	ecb:100034229.
Organism-specific databases
CTD	1583.
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000013161.
HOVERGEN	HBG051098.
InParanoid	O46515.
KO	K00498.
OrthoDB	EOG4GXFMF.
Enzyme and pathway databases
UniPathway	UPA00229.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP11A_HORSE

Accession

Primary (citable) accession number: O46515

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program