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O46515 (CP11A_HORSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cholesterol side-chain cleavage enzyme, mitochondrial

EC=1.14.15.6
Alternative name(s):
CYPXIA1
Cholesterol desmolase
Cytochrome P450 11A1
Cytochrome P450(scc)
Gene names
Name:CYP11A1
OrganismEquus caballus (Horse) [Reference proteome]
Taxonomic identifier9796 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus

Protein attributes

Sequence length520 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.

Catalytic activity

Cholesterol + 6 reduced adrenodoxin + 3 O2 = pregnenolone + 4-methylpentanal + 6 oxidized adrenodoxin + 4 H2O.

Cofactor

Heme group By similarity.

Pathway

Lipid metabolism; C21-steroid hormone metabolism.

Subunit structure

Interacts with FDX1/adrenodoxin By similarity.

Subcellular location

Mitochondrion membrane.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3939Mitochondrion By similarity
Chain40 – 520481Cholesterol side-chain cleavage enzyme, mitochondrial
PRO_0000003584

Sites

Metal binding4611Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
O46515 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: B8420BF587850192

FASTA52060,314
        10         20         30         40         50         60 
MLVRGLPLRS VLVKGCQPLL SAPREGPGHP RVPTGEGAGM SSHSPRPFKE IPSPGDNGWI 

        70         80         90        100        110        120 
NLYHFWREKG PKKLHYHHFQ NFQKYGPIYR EKLGNVESVY IVDPEDVALL FKFEGPHPER 

       130        140        150        160        170        180 
FLIPPWTAYH QYFQKPVGVL FKSSDAWKKD RLALNPEVMA LESIKNFIPL LDPVSQDFVS 

       190        200        210        220        230        240 
LLHRRMEQQG SGKFSGPIIE DLFRFAFESI TNVIFGERQG MLDEIVDPEA QRFIDAVYKM 

       250        260        270        280        290        300 
FHTSVPMLSL PPDLFRLFRT KTWRDHVAAW DTVFSKAEQY TEKFYQDLKQ KRHFDSYPGI 

       310        320        330        340        350        360 
FYRLLASNKL PFKDIQANVT EMLAGGVDTT SMSLQWHLYE IARNLRVQEM LREEVLAARR 

       370        380        390        400        410        420 
QAQGDTSTMV QMVPLLKASI KETLRLHPIA VTLQRYPQND LVIRDYMIPA KTLVQVSIYT 

       430        440        450        460        470        480 
MGQDPTFFSN PRRFDPTRWL DKNKDLTHFR NLGFGWGVRQ CLGRRIAELE MTLFLIHILE 

       490        500        510        520 
NFRVEIQHLN DVDSTFGLIL IPEKPISFTF WPITRAPPQA 

« Hide

References

[1]"Equine P450 cholesterol side-chain cleavage and 3 beta-hydroxysteroid dehydrogenase/delta(5)-delta(4) isomerase: molecular cloning and regulation of their messenger ribonucleic acids in equine follicles during the ovulatory process."
Boerboom D., Sirois J.
Biol. Reprod. 64:206-215(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Ovarian follicle membrane.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF031664 Genomic DNA. Translation: AAC04700.1.
RefSeqNP_001075990.1. NM_001082521.1.
UniGeneEca.13386.

3D structure databases

ProteinModelPortalO46515.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9796.ENSECAP00000015487.

Proteomic databases

PRIDEO46515.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100034229.
KEGGecb:100034229.

Organism-specific databases

CTD1583.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000013161.
HOVERGENHBG051098.
InParanoidO46515.
KOK00498.

Enzyme and pathway databases

UniPathwayUPA00229.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP11A_HORSE
AccessionPrimary (citable) accession number: O46515
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: July 9, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways