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O46512 (CP19A_HORSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Aromatase

EC=1.14.14.14
Alternative name(s):
CYPXIX
Cytochrome P-450AROM
Cytochrome P450 17-alpha
Cytochrome P450 19A1
Estrogen synthase
Gene names
Name:CYP19A1
Synonyms:CYP19
OrganismEquus caballus (Horse) [Reference proteome]
Taxonomic identifier9796 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Final rate-limiting step.

Catalytic activity

Testosterone + 3 O2 + 3 reduced flavoproteins = 17-beta-estradiol + formate + 4 H2O + 3 oxidized flavoproteins.

Androst-4-ene-3,17-dione + 3 O2 + 3 reduced flavoproteins = estrone + formate + 4 H2O + 3 oxidized flavoproteins.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein By similarity.

Tissue specificity

Expressed in placenta. Highly expressed in follicles (0 hour:hCG), followed by a drop (12-24 hour:hCG) and by an increase (30-39 hour:hCG). Highly expressed in corpora lutea. Also expressed in granulosa cell layer. Not expressed in theca interna.

Domain

Contains a I helix thought to serve as the substrate-binding pocket.

Sequence similarities

Belongs to the cytochrome P450 family.

Caution

Clone A1 form may be a splice variant or an artifact.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Aromatase
PRO_0000051954

Regions

Region294 – 32431Substrate-binding pocket Potential

Sites

Metal binding4371Iron (heme axial ligand) By similarity
Binding site3091Substrate By similarity
Binding site3741Substrate; via amide nitrogen By similarity

Experimental info

Sequence conflict342 – 3476ERDLKN → RNLSNK clone A1 Ref.1
Sequence conflict348 – 503156Missing clone A1 Ref.1

Sequences

Sequence LengthMass (Da)Tools
O46512 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 878E2CB040B5AECE

FASTA50357,825
        10         20         30         40         50         60 
MILEMLNPMH YNLTSMVPEV MPVATLPILL LTGFLFFVWN HEETSSIPGP GYCMGIGPLI 

        70         80         90        100        110        120 
SHLRFLWMGL GSACNYYNKM YGEFVRVWIS GEETLVISKS SSTFHIMKHD HYSSRFGSTF 

       130        140        150        160        170        180 
GLQYMGMHEN GVIFNNNPAV WKALRPFFVK ALSGPSLARM VTVCVESVNN HLDRLDEVTN 

       190        200        210        220        230        240 
ALGHVNVLTL MRRTMLDASN TLFLRIPLDE KNIVLKIQGY FDAWQALLIK PNIFFKISWL 

       250        260        270        280        290        300 
SRKHQKSIKE LRDAVGILAE EKRHRIFTAE KLEDHVDFAT DLILAEKRGE LTKENVNQCI 

       310        320        330        340        350        360 
LEMMIAAPDT LSVTVFFMLC LIAQHPKVEE ALMKEIQTVL GERDLKNDDM QKLKVMENFI 

       370        380        390        400        410        420 
NESMRYQPVV DIVMRKALED DVIDGYPVKK GTNIILNIGR MHKLEFFPKP NEFTLENFEK 

       430        440        450        460        470        480 
NVPYRYFQPF GFGPRSCAGK FIAMVMMKVM LVSLLRRFHV KTLQGNCLEN MQKTNDLALH 

       490        500 
PDESRSLPAM IFTPRNSEKC LEH 

« Hide

References

[1]"Dual regulation of promoter II- and promoter 1f-derived cytochrome P450 aromatase transcripts in equine granulosa cells during human chorionic gonadotropin-induced ovulation: a novel model for the study of aromatase promoter switching."
Boerboom D., Kerban A., Sirois J.
Endocrinology 140:4133-4141(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (CLONE A1 AND CLONE A17).
Tissue: Follicular cell.
[2]"Molecular characterization and expression of equine testicular cytochrome P450 aromatase."
Seralini G.E., Tomilin A., Auvray P., Nativelle-Serpentini C., Sourdaine P., Moslemi S.
Biochim. Biophys. Acta 1625:229-238(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF031520 mRNA. Translation: AAC04698.1.
AF031521 mRNA. Translation: AAC04699.1.
AJ012610 mRNA. Translation: CAB38442.1.
RefSeqNP_001075274.1. NM_001081805.1.
XP_005602644.1. XM_005602587.1.
XP_005602645.1. XM_005602588.1.
UniGeneEca.11648.

3D structure databases

ProteinModelPortalO46512.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9796.ENSECAP00000018821.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSECAT00000022758; ENSECAP00000018821; ENSECAG00000020474.
GeneID100009712.
KEGGecb:100009712.

Organism-specific databases

CTD1588.

Phylogenomic databases

eggNOGCOG2124.
GeneTreeENSGT00750000117408.
HOGENOMHOG000111912.
HOVERGENHBG050750.
InParanoidO46512.
KOK07434.
OMANFAKNVP.
OrthoDBEOG7D85W5.
TreeFamTF352039.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP19A_HORSE
AccessionPrimary (citable) accession number: O46512
Secondary accession number(s): O46513
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: May 14, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families