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O46512

- CP19A_HORSE

UniProt

O46512 - CP19A_HORSE

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Protein

Aromatase

Gene

CYP19A1

Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Final rate-limiting step.

Catalytic activityi

Testosterone + 3 O2 + 3 reduced flavoproteins = 17-beta-estradiol + formate + 4 H2O + 3 oxidized flavoproteins.
Androst-4-ene-3,17-dione + 3 O2 + 3 reduced flavoproteins = estrone + formate + 4 H2O + 3 oxidized flavoproteins.

Cofactori

Heme group.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei309 – 3091SubstrateBy similarity
Binding sitei374 – 3741Substrate; via amide nitrogenBy similarity
Metal bindingi437 – 4371Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB
  2. heme binding Source: UniProtKB
  3. iron ion binding Source: InterPro

GO - Biological processi

  1. androgen metabolic process Source: Ensembl
  2. prostate gland growth Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aromatase (EC:1.14.14.14)
Alternative name(s):
CYPXIX
Cytochrome P-450AROM
Cytochrome P450 17-alpha
Cytochrome P450 19A1
Estrogen synthase
Gene namesi
Name:CYP19A1
Synonyms:CYP19
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
ProteomesiUP000002281: Chromosome 1

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 503503AromatasePRO_0000051954Add
BLAST

Expressioni

Tissue specificityi

Expressed in placenta. Highly expressed in follicles (0 hour:hCG), followed by a drop (12-24 hour:hCG) and by an increase (30-39 hour:hCG). Highly expressed in corpora lutea. Also expressed in granulosa cell layer. Not expressed in theca interna.

Interactioni

Protein-protein interaction databases

STRINGi9796.ENSECAP00000018821.

Structurei

3D structure databases

ProteinModelPortaliO46512.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni294 – 32431Substrate-binding pocketSequence AnalysisAdd
BLAST

Domaini

Contains a I helix thought to serve as the substrate-binding pocket.

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000119376.
HOGENOMiHOG000111912.
HOVERGENiHBG050750.
InParanoidiO46512.
KOiK07434.
OMAiNFAKNVP.
OrthoDBiEOG7D85W5.
TreeFamiTF352039.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O46512-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MILEMLNPMH YNLTSMVPEV MPVATLPILL LTGFLFFVWN HEETSSIPGP
60 70 80 90 100
GYCMGIGPLI SHLRFLWMGL GSACNYYNKM YGEFVRVWIS GEETLVISKS
110 120 130 140 150
SSTFHIMKHD HYSSRFGSTF GLQYMGMHEN GVIFNNNPAV WKALRPFFVK
160 170 180 190 200
ALSGPSLARM VTVCVESVNN HLDRLDEVTN ALGHVNVLTL MRRTMLDASN
210 220 230 240 250
TLFLRIPLDE KNIVLKIQGY FDAWQALLIK PNIFFKISWL SRKHQKSIKE
260 270 280 290 300
LRDAVGILAE EKRHRIFTAE KLEDHVDFAT DLILAEKRGE LTKENVNQCI
310 320 330 340 350
LEMMIAAPDT LSVTVFFMLC LIAQHPKVEE ALMKEIQTVL GERDLKNDDM
360 370 380 390 400
QKLKVMENFI NESMRYQPVV DIVMRKALED DVIDGYPVKK GTNIILNIGR
410 420 430 440 450
MHKLEFFPKP NEFTLENFEK NVPYRYFQPF GFGPRSCAGK FIAMVMMKVM
460 470 480 490 500
LVSLLRRFHV KTLQGNCLEN MQKTNDLALH PDESRSLPAM IFTPRNSEKC

LEH
Length:503
Mass (Da):57,825
Last modified:June 1, 1998 - v1
Checksum:i878E2CB040B5AECE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti342 – 3476ERDLKN → RNLSNK clone A1 (PubMed:10465286)Curated
Sequence conflicti348 – 503156Missing clone A1 (PubMed:10465286)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF031520 mRNA. Translation: AAC04698.1.
AF031521 mRNA. Translation: AAC04699.1.
AJ012610 mRNA. Translation: CAB38442.1.
RefSeqiNP_001075274.1. NM_001081805.1.
XP_005602644.1. XM_005602587.1.
XP_005602645.1. XM_005602588.1.
UniGeneiEca.11648.

Genome annotation databases

EnsembliENSECAT00000022758; ENSECAP00000018821; ENSECAG00000020474.
GeneIDi100009712.
KEGGiecb:100009712.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF031520 mRNA. Translation: AAC04698.1 .
AF031521 mRNA. Translation: AAC04699.1 .
AJ012610 mRNA. Translation: CAB38442.1 .
RefSeqi NP_001075274.1. NM_001081805.1.
XP_005602644.1. XM_005602587.1.
XP_005602645.1. XM_005602588.1.
UniGenei Eca.11648.

3D structure databases

ProteinModelPortali O46512.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9796.ENSECAP00000018821.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSECAT00000022758 ; ENSECAP00000018821 ; ENSECAG00000020474 .
GeneIDi 100009712.
KEGGi ecb:100009712.

Organism-specific databases

CTDi 1588.

Phylogenomic databases

eggNOGi COG2124.
GeneTreei ENSGT00760000119376.
HOGENOMi HOG000111912.
HOVERGENi HBG050750.
InParanoidi O46512.
KOi K07434.
OMAi NFAKNVP.
OrthoDBi EOG7D85W5.
TreeFami TF352039.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Dual regulation of promoter II- and promoter 1f-derived cytochrome P450 aromatase transcripts in equine granulosa cells during human chorionic gonadotropin-induced ovulation: a novel model for the study of aromatase promoter switching."
    Boerboom D., Kerban A., Sirois J.
    Endocrinology 140:4133-4141(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (CLONE A1 AND CLONE A17).
    Tissue: Follicular cell.
  2. "Molecular characterization and expression of equine testicular cytochrome P450 aromatase."
    Seralini G.E., Tomilin A., Auvray P., Nativelle-Serpentini C., Sourdaine P., Moslemi S.
    Biochim. Biophys. Acta 1625:229-238(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiCP19A_HORSE
AccessioniPrimary (citable) accession number: O46512
Secondary accession number(s): O46513
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Clone A1 form may be a splice variant or an artifact.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3