Reviewed, UniProtKB/Swiss-Prot O46512 (CP19A_HORSE)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 19A1
    EC=1.14.14.1
Alternative name(s):
    CYPXIX
    Aromatase
    Estrogen synthase
    Cytochrome P-450AROM
    Cytochrome P450 17-alpha

Gene names

Name:	CYP19A1
Synonyms:	CYP19

Organism

Equus caballus (Horse)

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Final rate-limiting step.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein By similarity.
Tissue specificity	Expressed in placenta. Highly expressed in follicles (0 hour:hCG), followed by a drop (12-24 hour:hCG) and by an increase (30-39 hour:hCG). Highly expressed in corpora lutea. Also expressed in granulosa cell layer. Not expressed in theca interna.
Domain	Contains a I helix thought to serve as the substrate-binding pocket.
Sequence similarities	Belongs to the cytochrome P450 family.
Caution	Clone A1 form may be a splice variant or an artifact.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Cytochrome P450 19A1		PRO_0000051954
Regions
	Region	294 – 324	31	Substrate-binding pocket Potential
Sites
	Metal binding	437	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Modified residue	162	1	Phosphothreonine By similarity
Experimental info
	Sequence conflict	342 – 347	6	ERDLKN → RNLSNK clone A1 Ref.1
	Sequence conflict	348 – 503	156	Missing clone A1 Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O46512-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 878E2CB040B5AECE	FASTA	503	57,825
	10 20 30 40 50 60 MILEMLNPMH YNLTSMVPEV MPVATLPILL LTGFLFFVWN HEETSSIPGP GYCMGIGPLI 70 80 90 100 110 120 SHLRFLWMGL GSACNYYNKM YGEFVRVWIS GEETLVISKS SSTFHIMKHD HYSSRFGSTF 130 140 150 160 170 180 GLQYMGMHEN GVIFNNNPAV WKALRPFFVK ALSGPSLARM VTVCVESVNN HLDRLDEVTN 190 200 210 220 230 240 ALGHVNVLTL MRRTMLDASN TLFLRIPLDE KNIVLKIQGY FDAWQALLIK PNIFFKISWL 250 260 270 280 290 300 SRKHQKSIKE LRDAVGILAE EKRHRIFTAE KLEDHVDFAT DLILAEKRGE LTKENVNQCI 310 320 330 340 350 360 LEMMIAAPDT LSVTVFFMLC LIAQHPKVEE ALMKEIQTVL GERDLKNDDM QKLKVMENFI 370 380 390 400 410 420 NESMRYQPVV DIVMRKALED DVIDGYPVKK GTNIILNIGR MHKLEFFPKP NEFTLENFEK 430 440 450 460 470 480 NVPYRYFQPF GFGPRSCAGK FIAMVMMKVM LVSLLRRFHV KTLQGNCLEN MQKTNDLALH 490 500 PDESRSLPAM IFTPRNSEKC LEH

References

[1]

"Dual regulation of promoter II- and promoter 1f-derived cytochrome P450 aromatase transcripts in equine granulosa cells during human chorionic gonadotropin-induced ovulation: a novel model for the study of aromatase promoter switching." Boerboom D., Kerban A., Sirois J. Endocrinology 140:4133-4141(1999) [PubMed: 10465286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (CLONE A1 AND CLONE A17). Tissue: Follicular cell.

[2]

"Molecular characterization and expression of equine testicular cytochrome P450 aromatase." Seralini G.E., Tomilin A., Auvray P., Nativelle-Serpentini C., Sourdaine P., Moslemi S. Biochim. Biophys. Acta 1625:229-238(2003) [PubMed: 12591609] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF031520 mRNA. Translation: AAC04698.1. AF031521 mRNA. Translation: AAC04699.1. AJ012610 mRNA. Translation: CAB38442.1.
RefSeq	NP_001075274.1.
UniGene	Eca.11648
3D structure databases
SMR	O46512. Positions 45-467, 78-496.
ModBase	Search...
Genome annotation databases
Ensembl	ENSECAT00000022758; ENSECAP00000018821; ENSECAG00000020474; Equus caballus. [Genome view]
GeneID	100009712.
KEGG	ecb:100009712.
Organism-specific databases
CTD	100009712.
Phylogenomic databases
HOVERGEN	O46512.
InParanoid	O46512.
OMA	TRENVNQ.
OrthoDB	EOG9F20P2.
Enzyme and pathway databases
BRENDA	1.14.14.1. 1464.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP19A_HORSE

Accession

Primary (citable) accession number: O46512
Secondary accession number(s): O46513

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	February 9, 2010
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)