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O46512

- CP19A_HORSE

UniProt

O46512 - CP19A_HORSE

Protein

Aromatase

Gene

CYP19A1

Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Final rate-limiting step.

    Catalytic activityi

    Testosterone + 3 O2 + 3 reduced flavoproteins = 17-beta-estradiol + formate + 4 H2O + 3 oxidized flavoproteins.
    Androst-4-ene-3,17-dione + 3 O2 + 3 reduced flavoproteins = estrone + formate + 4 H2O + 3 oxidized flavoproteins.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei309 – 3091SubstrateBy similarity
    Binding sitei374 – 3741Substrate; via amide nitrogenBy similarity
    Metal bindingi437 – 4371Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB
    2. heme binding Source: UniProtKB
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. androgen metabolic process Source: Ensembl
    2. prostate gland growth Source: Ensembl

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aromatase (EC:1.14.14.14)
    Alternative name(s):
    CYPXIX
    Cytochrome P-450AROM
    Cytochrome P450 17-alpha
    Cytochrome P450 19A1
    Estrogen synthase
    Gene namesi
    Name:CYP19A1
    Synonyms:CYP19
    OrganismiEquus caballus (Horse)
    Taxonomic identifieri9796 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
    ProteomesiUP000002281: Chromosome 1

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 503503AromatasePRO_0000051954Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in placenta. Highly expressed in follicles (0 hour:hCG), followed by a drop (12-24 hour:hCG) and by an increase (30-39 hour:hCG). Highly expressed in corpora lutea. Also expressed in granulosa cell layer. Not expressed in theca interna.

    Interactioni

    Protein-protein interaction databases

    STRINGi9796.ENSECAP00000018821.

    Structurei

    3D structure databases

    ProteinModelPortaliO46512.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni294 – 32431Substrate-binding pocketSequence AnalysisAdd
    BLAST

    Domaini

    Contains a I helix thought to serve as the substrate-binding pocket.

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00750000117408.
    HOGENOMiHOG000111912.
    HOVERGENiHBG050750.
    InParanoidiO46512.
    KOiK07434.
    OMAiNFAKNVP.
    OrthoDBiEOG7D85W5.
    TreeFamiTF352039.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O46512-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILEMLNPMH YNLTSMVPEV MPVATLPILL LTGFLFFVWN HEETSSIPGP    50
    GYCMGIGPLI SHLRFLWMGL GSACNYYNKM YGEFVRVWIS GEETLVISKS 100
    SSTFHIMKHD HYSSRFGSTF GLQYMGMHEN GVIFNNNPAV WKALRPFFVK 150
    ALSGPSLARM VTVCVESVNN HLDRLDEVTN ALGHVNVLTL MRRTMLDASN 200
    TLFLRIPLDE KNIVLKIQGY FDAWQALLIK PNIFFKISWL SRKHQKSIKE 250
    LRDAVGILAE EKRHRIFTAE KLEDHVDFAT DLILAEKRGE LTKENVNQCI 300
    LEMMIAAPDT LSVTVFFMLC LIAQHPKVEE ALMKEIQTVL GERDLKNDDM 350
    QKLKVMENFI NESMRYQPVV DIVMRKALED DVIDGYPVKK GTNIILNIGR 400
    MHKLEFFPKP NEFTLENFEK NVPYRYFQPF GFGPRSCAGK FIAMVMMKVM 450
    LVSLLRRFHV KTLQGNCLEN MQKTNDLALH PDESRSLPAM IFTPRNSEKC 500
    LEH 503
    Length:503
    Mass (Da):57,825
    Last modified:June 1, 1998 - v1
    Checksum:i878E2CB040B5AECE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti342 – 3476ERDLKN → RNLSNK clone A1 (PubMed:10465286)Curated
    Sequence conflicti348 – 503156Missing clone A1 (PubMed:10465286)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031520 mRNA. Translation: AAC04698.1.
    AF031521 mRNA. Translation: AAC04699.1.
    AJ012610 mRNA. Translation: CAB38442.1.
    RefSeqiNP_001075274.1. NM_001081805.1.
    XP_005602644.1. XM_005602587.1.
    XP_005602645.1. XM_005602588.1.
    UniGeneiEca.11648.

    Genome annotation databases

    EnsembliENSECAT00000022758; ENSECAP00000018821; ENSECAG00000020474.
    GeneIDi100009712.
    KEGGiecb:100009712.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031520 mRNA. Translation: AAC04698.1 .
    AF031521 mRNA. Translation: AAC04699.1 .
    AJ012610 mRNA. Translation: CAB38442.1 .
    RefSeqi NP_001075274.1. NM_001081805.1.
    XP_005602644.1. XM_005602587.1.
    XP_005602645.1. XM_005602588.1.
    UniGenei Eca.11648.

    3D structure databases

    ProteinModelPortali O46512.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9796.ENSECAP00000018821.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSECAT00000022758 ; ENSECAP00000018821 ; ENSECAG00000020474 .
    GeneIDi 100009712.
    KEGGi ecb:100009712.

    Organism-specific databases

    CTDi 1588.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00750000117408.
    HOGENOMi HOG000111912.
    HOVERGENi HBG050750.
    InParanoidi O46512.
    KOi K07434.
    OMAi NFAKNVP.
    OrthoDBi EOG7D85W5.
    TreeFami TF352039.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Dual regulation of promoter II- and promoter 1f-derived cytochrome P450 aromatase transcripts in equine granulosa cells during human chorionic gonadotropin-induced ovulation: a novel model for the study of aromatase promoter switching."
      Boerboom D., Kerban A., Sirois J.
      Endocrinology 140:4133-4141(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (CLONE A1 AND CLONE A17).
      Tissue: Follicular cell.
    2. "Molecular characterization and expression of equine testicular cytochrome P450 aromatase."
      Seralini G.E., Tomilin A., Auvray P., Nativelle-Serpentini C., Sourdaine P., Moslemi S.
      Biochim. Biophys. Acta 1625:229-238(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiCP19A_HORSE
    AccessioniPrimary (citable) accession number: O46512
    Secondary accession number(s): O46513
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    Clone A1 form may be a splice variant or an artifact.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3