ID   CP7A1_PIG               Reviewed;         501 AA.
AC   O46491;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   16-JUN-2009, entry version 56.
DE   RecName: Full=Cholesterol 7-alpha-monooxygenase;
DE            EC=1.14.13.17;
DE   AltName: Full=Cytochrome P450 7A1;
DE   AltName: Full=CYPVII;
DE   AltName: Full=Cholesterol 7-alpha-hydroxylase;
GN   Name=CYP7A1; Synonyms=CYP7;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae;
OC   Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Ishimura-Oka K., Li C.M., Chang B.H.J., Pond W.G., Oka K., Chan L.;
RT   "Structure of the porcine cholesterol 7 alpha-hydroxylase gene.";
RL   Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: Cholesterol + NADPH + O(2) = 7-alpha-
CC       hydroxycholesterol + NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Lipid metabolism; bile acid biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral
CC       membrane protein. Microsome membrane; Peripheral membrane protein.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; AF020322; AAC04676.1; -; Genomic_DNA.
DR   EMBL; AF020317; AAC04676.1; JOINED; Genomic_DNA.
DR   EMBL; AF020318; AAC04676.1; JOINED; Genomic_DNA.
DR   EMBL; AF020319; AAC04676.1; JOINED; Genomic_DNA.
DR   EMBL; AF020320; AAC04676.1; JOINED; Genomic_DNA.
DR   EMBL; AF020321; AAC04676.1; JOINED; Genomic_DNA.
DR   RefSeq; NP_001005352.1; -.
DR   UniGene; Ssc.30521; -.
DR   GeneID; 448985; -.
DR   KEGG; ssc:448985; -.
DR   HOVERGEN; O46491; -.
DR   BRENDA; 1.14.13.17; 249.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005792; C:microsome; IEA:UniProtKB-SubCell.
DR   GO; GO:0008123; F:cholesterol 7-alpha-monooxygenase activity; IEA:EC.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0008203; P:cholesterol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017973; Cyt_P450_C.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002403; Cyt_P450_E_grp-IV.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00465; EP450IV.
DR   PRINTS; PR00385; P450.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   3: Inferred from homology;
KW   Cholesterol metabolism; Endoplasmic reticulum; Heme; Iron;
KW   Lipid metabolism; Membrane; Metal-binding; Microsome; Monooxygenase;
KW   NADP; Oxidoreductase; Steroid metabolism.
FT   CHAIN         1    501       Cholesterol 7-alpha-monooxygenase.
FT                                /FTId=PRO_0000051903.
FT   METAL       441    441       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   501 AA;  56846 MW;  7B56BEDC4371BFED CRC64;
     MMSISLLGGI VTAVCCCLWL LLGMRRRQTG EPPLENGIIP YLGCALQFGA NPLEFLRANQ
     RKHGHIFTCQ LMGNYVHFIT NPLSYHKVLC HGKYLDWKKF HFTASAKAFG HRSIDPSDGN
     TTDNINKTII KTLQGDALNL LAAAMMENLQ LVLRPQVAPQ PEKPAWVTEG MYSFCYRVMF
     EAGYVTLFGK DPIGHDAQKA LILNNLDNFK QFDKIFPALV AGFPIHVFKT GHYAREKLAE
     GLRLQKLRKR DHISELVRFL NDTLSTLDDA EKAKSLLAVL WASQANTIPA TFWCLFQTIR
     SPEAMKAASE EVNKTLEKAG QKISLDDKPI YLNQIELDSM PVLDSIIKES LRLSSASLNI
     RTAKEDFTLH LQDGSYNIRK DDIIALYPQL MHLDPEIYPD PLTFKYDRYL DENGKTKTTF
     YSHGLKLKYY YMPFGSGATI CPGRLFAVQE IKQFLILMLS YFDLELVESH VKCPPLDQSR
     AGLGILPPSN DIEFRYKLKH L
//