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Protein

Cholesterol 7-alpha-monooxygenase

Gene

CYP7A1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.By similarity

Catalytic activityi

Cholesterol + [reduced NADPH--hemoprotein reductase] + O2 = 7-alpha-hydroxycholesterol + [oxidized NADPH--hemoprotein reductase] + H2O.By similarity

Cofactori

hemeBy similarity

Pathwayi: bile acid biosynthesis

This protein is involved in the pathway bile acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway bile acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi441Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Cholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00221.

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol 7-alpha-monooxygenase (EC:1.14.14.23By similarity)
Alternative name(s):
CYPVII
Cholesterol 7-alpha-hydroxylase
Cytochrome P450 7A1
Gene namesi
Name:CYP7A1
Synonyms:CYP7
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519031 – 501Cholesterol 7-alpha-monooxygenaseAdd BLAST501

Proteomic databases

PaxDbiO46491.
PRIDEiO46491.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000006650.

Structurei

3D structure databases

ProteinModelPortaliO46491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiO46491.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR030681. Cholesterol_7a_monooxygenase.
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PANTHERiPTHR24304:SF1. PTHR24304:SF1. 1 hit.
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF500625. Cytochrome_CYP7A1. 1 hit.
PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O46491-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSISLLGGI VTAVCCCLWL LLGMRRRQTG EPPLENGIIP YLGCALQFGA
60 70 80 90 100
NPLEFLRANQ RKHGHIFTCQ LMGNYVHFIT NPLSYHKVLC HGKYLDWKKF
110 120 130 140 150
HFTASAKAFG HRSIDPSDGN TTDNINKTII KTLQGDALNL LAAAMMENLQ
160 170 180 190 200
LVLRPQVAPQ PEKPAWVTEG MYSFCYRVMF EAGYVTLFGK DPIGHDAQKA
210 220 230 240 250
LILNNLDNFK QFDKIFPALV AGFPIHVFKT GHYAREKLAE GLRLQKLRKR
260 270 280 290 300
DHISELVRFL NDTLSTLDDA EKAKSLLAVL WASQANTIPA TFWCLFQTIR
310 320 330 340 350
SPEAMKAASE EVNKTLEKAG QKISLDDKPI YLNQIELDSM PVLDSIIKES
360 370 380 390 400
LRLSSASLNI RTAKEDFTLH LQDGSYNIRK DDIIALYPQL MHLDPEIYPD
410 420 430 440 450
PLTFKYDRYL DENGKTKTTF YSHGLKLKYY YMPFGSGATI CPGRLFAVQE
460 470 480 490 500
IKQFLILMLS YFDLELVESH VKCPPLDQSR AGLGILPPSN DIEFRYKLKH

L
Length:501
Mass (Da):56,846
Last modified:June 1, 1998 - v1
Checksum:i7B56BEDC4371BFED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020322
, AF020317, AF020318, AF020319, AF020320, AF020321 Genomic DNA. Translation: AAC04676.1.
UniGeneiSsc.30521.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020322
, AF020317, AF020318, AF020319, AF020320, AF020321 Genomic DNA. Translation: AAC04676.1.
UniGeneiSsc.30521.

3D structure databases

ProteinModelPortaliO46491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000006650.

Proteomic databases

PaxDbiO46491.
PRIDEiO46491.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000231026.
HOVERGENiHBG051100.
InParanoidiO46491.

Enzyme and pathway databases

UniPathwayiUPA00221.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR030681. Cholesterol_7a_monooxygenase.
IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR024204. Cyt_P450_CYP7A1-type.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PANTHERiPTHR24304:SF1. PTHR24304:SF1. 1 hit.
PfamiPF00067. p450. 1 hit.
[Graphical view]
PIRSFiPIRSF500625. Cytochrome_CYP7A1. 1 hit.
PIRSF000047. Cytochrome_CYPVIIA1. 1 hit.
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP7A1_PIG
AccessioniPrimary (citable) accession number: O46491
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.