SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O46432

- MA2B1_FELCA

UniProt

O46432 - MA2B1_FELCA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Lysosomal alpha-mannosidase

Gene
MAN2B1, MANB
Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover By similarity.

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi73 – 731Zinc By similarity
Metal bindingi75 – 751Zinc By similarity
Active sitei197 – 1971Nucleophile By similarity
Metal bindingi197 – 1971Zinc By similarity
Metal bindingi448 – 4481Zinc By similarity

GO - Molecular functioni

  1. alpha-mannosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. mannose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal alpha-mannosidase (EC:3.2.1.24)
Short name:
Laman
Alternative name(s):
Lysosomal acid alpha-mannosidase
Mannosidase alpha class 2B member 1
Mannosidase alpha-B
Gene namesi
Name:MAN2B1
Synonyms:MANB
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
ProteomesiUP000011712: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. lysosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Involvement in diseasei

Defects in MAN2B1 are the cause of lysosomal alpha-mannosidosis (AM). AM is a lysosomal storage disease characterized by accumulation of unbranched oligosaccharide chains.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5050 By similarityAdd
BLAST
Chaini51 – 1007957Lysosomal alpha-mannosidasePRO_0000012068Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi56 ↔ 360 By similarity
Glycosylationi134 – 1341N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi269 ↔ 274 By similarity
Glycosylationi311 – 3111N-linked (GlcNAc...) Reviewed prediction
Glycosylationi347 – 3471N-linked (GlcNAc...) Reviewed prediction
Glycosylationi369 – 3691N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi414 ↔ 474 By similarity
Disulfide bondi495 ↔ 503 By similarity
Glycosylationi499 – 4991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi543 – 5431N-linked (GlcNAc...) Reviewed prediction
Glycosylationi643 – 6431N-linked (GlcNAc...) Reviewed prediction
Glycosylationi649 – 6491N-linked (GlcNAc...) Reviewed prediction
Glycosylationi690 – 6901N-linked (GlcNAc...) Reviewed prediction
Glycosylationi764 – 7641N-linked (GlcNAc...) Reviewed prediction
Glycosylationi927 – 9271N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Processed into 3 peptides of 72 kDa, 41 kDa and 12 kDa.

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliO46432.
SMRiO46432. Positions 52-342, 350-423, 433-582, 601-872, 883-1003.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG052391.
KOiK12311.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O46432-1 [UniParc]FASTAAdd to Basket

« Hide

MGADARPLGV RAGGGGRGAA RPGTSSRALP PPLPPLSFLL LLLAAPGARA     50
AGYETCPMVH PDMLNVHLVA HTHDDVGWLK TVDQYFYGIH NDVQHAGVQY 100
ILDSVISSLL VEPTRRFIYV EIAFFSRWWH QQTNATQEVV RDLVRQGRLE 150
FANGGWVMND EAATHYGAII DQMTLGLRFL EDTFGKDGRP RVAWHIDPFG 200
HSREQASLFA QMGFDGLFFG RLDYQDKRVR EENLGLEQVW RASASLKPPA 250
ADLFTSVLPN IYNPPEKLCW DTLCADKPFV EDRRSPEYNA EELVNYFLQL 300
ATAQGQHFRT NHTIMTMGSD FQYENANMWF RNLDRLIQLV NAQQQANGSR 350
VNVLYSTPAC YLWELNKANL TWSVKQDDFF PYADGPHQFW SGYFSSRPAL 400
KRYERLSYNF LQVCNQLEAL AGPAANVGPY GSGDSAPLNQ AMAVLQHHDA 450
VSGTSKQHVA DDYARQLAAG WDPCEVLLSN ALARLSGSKE DFTYCRNLNV 500
SVCPLSQTAK NFQVTIYNPL GRKIDWMVRL PVSKHGFVVR DPNGTVVPSD 550
VVILPSSDGQ ELLFPASVPA LGFSIYSVSQ VPGQRPHAHK PQPRSQRPWS 600
RVLAIQNEHI RARFDPDTGL LVEMENLDQN LLLPVRQAFY WYNASVGNNL 650
STQVSGAYIF RPNQEKPLMV SHWAQTRLVK TPLVQEVHQN FSAWCSQVVR 700
LYRGQRHLEL EWTVGPIPVG DGWGKEIISR FDTVLETKGL FYTDSNGREI 750
LERRRDYRPT WKLNQTETVA GNYYPVNSRI YIRDGNMQLT VLTDRSQGGS 800
SLRDGSMELM VHRRLLKDDG RGVGEALLED GLGRWVRGRH LVLLDKVRTA 850
ATGHRLQAEK EVLTPQVVLA PGGGAPYHLK VAPRKQFSGL RRELPPSVHL 900
LTLARWDQKT LLLRLEHQFA VGEDSGNLSS PVTLDLTDLF SAFTITYLQE 950
TTLVANQLRA SASRLKWTPN TGPTPLPSPS RLDPATITLQ PMEIRTFLAS 1000
VQWEEHG 1007
Length:1,007
Mass (Da):113,230
Last modified:June 1, 1998 - v1
Checksum:iC064A8168F5DC20A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF010191 mRNA. Translation: AAB97672.1.
AF010192 Genomic DNA. Translation: AAB97733.1.
PIRiT42219.
RefSeqiNP_001009222.1. NM_001009222.1.

Genome annotation databases

GeneIDi493697.
KEGGifca:493697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF010191 mRNA. Translation: AAB97672.1 .
AF010192 Genomic DNA. Translation: AAB97733.1 .
PIRi T42219.
RefSeqi NP_001009222.1. NM_001009222.1.

3D structure databases

ProteinModelPortali O46432.
SMRi O46432. Positions 52-342, 350-423, 433-582, 601-872, 883-1003.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH38. Glycoside Hydrolase Family 38.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 493697.
KEGGi fca:493697.

Organism-specific databases

CTDi 4125.

Phylogenomic databases

HOVERGENi HBG052391.
KOi K12311.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Purification of feline lysosomal alpha-mannosidase, determination of its cDNA sequence and identification of a mutation causing alpha-mannosidosis in Persian cats."
    Berg T., Tollersrud O.-K., Walkley S.U., Siegel D., Nilssen O.
    Biochem. J. 328:863-870(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], DISEASE.

Entry informationi

Entry nameiMA2B1_FELCA
AccessioniPrimary (citable) accession number: O46432
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi