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Protein

Lysosomal alpha-mannosidase

Gene

MAN2B1

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover.By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi73 – 731ZincBy similarity
Metal bindingi75 – 751ZincBy similarity
Active sitei197 – 1971NucleophileBy similarity
Metal bindingi197 – 1971ZincBy similarity
Metal bindingi448 – 4481ZincBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysosomal alpha-mannosidase (EC:3.2.1.24)
Short name:
Laman
Alternative name(s):
Lysosomal acid alpha-mannosidase
Mannosidase alpha class 2B member 1
Mannosidase alpha-B
Gene namesi
Name:MAN2B1
Synonyms:MANB
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
ProteomesiUP000011712 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Involvement in diseasei

Defects in MAN2B1 are the cause of lysosomal alpha-mannosidosis (AM). AM is a lysosomal storage disease characterized by accumulation of unbranched oligosaccharide chains.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5050By similarityAdd
BLAST
Chaini51 – 1007957Lysosomal alpha-mannosidasePRO_0000012068Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi56 ↔ 360By similarity
Glycosylationi134 – 1341N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi269 ↔ 274By similarity
Glycosylationi311 – 3111N-linked (GlcNAc...)Sequence Analysis
Glycosylationi347 – 3471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi369 – 3691N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi414 ↔ 474By similarity
Disulfide bondi495 ↔ 503By similarity
Glycosylationi499 – 4991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi543 – 5431N-linked (GlcNAc...)Sequence Analysis
Glycosylationi643 – 6431N-linked (GlcNAc...)Sequence Analysis
Glycosylationi649 – 6491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi690 – 6901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi764 – 7641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi927 – 9271N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Processed into 3 peptides of 72 kDa, 41 kDa and 12 kDa.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000020308.

Structurei

3D structure databases

ProteinModelPortaliO46432.
SMRiO46432. Positions 52-342, 350-423, 433-582, 601-872, 883-1003.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG052391.
InParanoidiO46432.
KOiK12311.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O46432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGADARPLGV RAGGGGRGAA RPGTSSRALP PPLPPLSFLL LLLAAPGARA
60 70 80 90 100
AGYETCPMVH PDMLNVHLVA HTHDDVGWLK TVDQYFYGIH NDVQHAGVQY
110 120 130 140 150
ILDSVISSLL VEPTRRFIYV EIAFFSRWWH QQTNATQEVV RDLVRQGRLE
160 170 180 190 200
FANGGWVMND EAATHYGAII DQMTLGLRFL EDTFGKDGRP RVAWHIDPFG
210 220 230 240 250
HSREQASLFA QMGFDGLFFG RLDYQDKRVR EENLGLEQVW RASASLKPPA
260 270 280 290 300
ADLFTSVLPN IYNPPEKLCW DTLCADKPFV EDRRSPEYNA EELVNYFLQL
310 320 330 340 350
ATAQGQHFRT NHTIMTMGSD FQYENANMWF RNLDRLIQLV NAQQQANGSR
360 370 380 390 400
VNVLYSTPAC YLWELNKANL TWSVKQDDFF PYADGPHQFW SGYFSSRPAL
410 420 430 440 450
KRYERLSYNF LQVCNQLEAL AGPAANVGPY GSGDSAPLNQ AMAVLQHHDA
460 470 480 490 500
VSGTSKQHVA DDYARQLAAG WDPCEVLLSN ALARLSGSKE DFTYCRNLNV
510 520 530 540 550
SVCPLSQTAK NFQVTIYNPL GRKIDWMVRL PVSKHGFVVR DPNGTVVPSD
560 570 580 590 600
VVILPSSDGQ ELLFPASVPA LGFSIYSVSQ VPGQRPHAHK PQPRSQRPWS
610 620 630 640 650
RVLAIQNEHI RARFDPDTGL LVEMENLDQN LLLPVRQAFY WYNASVGNNL
660 670 680 690 700
STQVSGAYIF RPNQEKPLMV SHWAQTRLVK TPLVQEVHQN FSAWCSQVVR
710 720 730 740 750
LYRGQRHLEL EWTVGPIPVG DGWGKEIISR FDTVLETKGL FYTDSNGREI
760 770 780 790 800
LERRRDYRPT WKLNQTETVA GNYYPVNSRI YIRDGNMQLT VLTDRSQGGS
810 820 830 840 850
SLRDGSMELM VHRRLLKDDG RGVGEALLED GLGRWVRGRH LVLLDKVRTA
860 870 880 890 900
ATGHRLQAEK EVLTPQVVLA PGGGAPYHLK VAPRKQFSGL RRELPPSVHL
910 920 930 940 950
LTLARWDQKT LLLRLEHQFA VGEDSGNLSS PVTLDLTDLF SAFTITYLQE
960 970 980 990 1000
TTLVANQLRA SASRLKWTPN TGPTPLPSPS RLDPATITLQ PMEIRTFLAS

VQWEEHG
Length:1,007
Mass (Da):113,230
Last modified:June 1, 1998 - v1
Checksum:iC064A8168F5DC20A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010191 mRNA. Translation: AAB97672.1.
AF010192 Genomic DNA. Translation: AAB97733.1.
PIRiT42219.
RefSeqiNP_001009222.1. NM_001009222.1.

Genome annotation databases

GeneIDi493697.
KEGGifca:493697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF010191 mRNA. Translation: AAB97672.1.
AF010192 Genomic DNA. Translation: AAB97733.1.
PIRiT42219.
RefSeqiNP_001009222.1. NM_001009222.1.

3D structure databases

ProteinModelPortaliO46432.
SMRiO46432. Positions 52-342, 350-423, 433-582, 601-872, 883-1003.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000020308.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi493697.
KEGGifca:493697.

Organism-specific databases

CTDi4125.

Phylogenomic databases

HOVERGENiHBG052391.
InParanoidiO46432.
KOiK12311.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Purification of feline lysosomal alpha-mannosidase, determination of its cDNA sequence and identification of a mutation causing alpha-mannosidosis in Persian cats."
    Berg T., Tollersrud O.-K., Walkley S.U., Siegel D., Nilssen O.
    Biochem. J. 328:863-870(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], DISEASE.

Entry informationi

Entry nameiMA2B1_FELCA
AccessioniPrimary (citable) accession number: O46432
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: June 24, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.