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O46432 (MA2B1_FELCA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysosomal alpha-mannosidase

Short name=Laman
EC=3.2.1.24
Alternative name(s):
Lysosomal acid alpha-mannosidase
Mannosidase alpha class 2B member 1
Mannosidase alpha-B
Gene names
Name:MAN2B1
Synonyms:MANB
OrganismFelis catus (Cat) (Felis silvestris catus) [Reference proteome]
Taxonomic identifier9685 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis

Protein attributes

Sequence length1007 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover By similarity.

Catalytic activity

Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Lysosome.

Post-translational modification

Processed into 3 peptides of 72 kDa, 41 kDa and 12 kDa.

Involvement in disease

Defects in MAN2B1 are the cause of lysosomal alpha-mannosidosis (AM). AM is a lysosomal storage disease characterized by accumulation of unbranched oligosaccharide chains. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 38 family.

Ontologies

Keywords
   Cellular componentLysosome
   DomainSignal
   LigandMetal-binding
Zinc
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmannose metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentlysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionalpha-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 5050 By similarity
Chain51 – 1007957Lysosomal alpha-mannosidase
PRO_0000012068

Sites

Active site1971Nucleophile By similarity
Metal binding731Zinc By similarity
Metal binding751Zinc By similarity
Metal binding1971Zinc By similarity
Metal binding4481Zinc By similarity

Amino acid modifications

Glycosylation1341N-linked (GlcNAc...) Potential
Glycosylation3111N-linked (GlcNAc...) Potential
Glycosylation3471N-linked (GlcNAc...) Potential
Glycosylation3691N-linked (GlcNAc...) Potential
Glycosylation4991N-linked (GlcNAc...) Potential
Glycosylation5431N-linked (GlcNAc...) Potential
Glycosylation6431N-linked (GlcNAc...) Potential
Glycosylation6491N-linked (GlcNAc...) Potential
Glycosylation6901N-linked (GlcNAc...) Potential
Glycosylation7641N-linked (GlcNAc...) Potential
Glycosylation9271N-linked (GlcNAc...) Potential
Disulfide bond56 ↔ 360 By similarity
Disulfide bond269 ↔ 274 By similarity
Disulfide bond414 ↔ 474 By similarity
Disulfide bond495 ↔ 503 By similarity

Sequences

Sequence LengthMass (Da)Tools
O46432 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: C064A8168F5DC20A

FASTA1,007113,230
        10         20         30         40         50         60 
MGADARPLGV RAGGGGRGAA RPGTSSRALP PPLPPLSFLL LLLAAPGARA AGYETCPMVH 

        70         80         90        100        110        120 
PDMLNVHLVA HTHDDVGWLK TVDQYFYGIH NDVQHAGVQY ILDSVISSLL VEPTRRFIYV 

       130        140        150        160        170        180 
EIAFFSRWWH QQTNATQEVV RDLVRQGRLE FANGGWVMND EAATHYGAII DQMTLGLRFL 

       190        200        210        220        230        240 
EDTFGKDGRP RVAWHIDPFG HSREQASLFA QMGFDGLFFG RLDYQDKRVR EENLGLEQVW 

       250        260        270        280        290        300 
RASASLKPPA ADLFTSVLPN IYNPPEKLCW DTLCADKPFV EDRRSPEYNA EELVNYFLQL 

       310        320        330        340        350        360 
ATAQGQHFRT NHTIMTMGSD FQYENANMWF RNLDRLIQLV NAQQQANGSR VNVLYSTPAC 

       370        380        390        400        410        420 
YLWELNKANL TWSVKQDDFF PYADGPHQFW SGYFSSRPAL KRYERLSYNF LQVCNQLEAL 

       430        440        450        460        470        480 
AGPAANVGPY GSGDSAPLNQ AMAVLQHHDA VSGTSKQHVA DDYARQLAAG WDPCEVLLSN 

       490        500        510        520        530        540 
ALARLSGSKE DFTYCRNLNV SVCPLSQTAK NFQVTIYNPL GRKIDWMVRL PVSKHGFVVR 

       550        560        570        580        590        600 
DPNGTVVPSD VVILPSSDGQ ELLFPASVPA LGFSIYSVSQ VPGQRPHAHK PQPRSQRPWS 

       610        620        630        640        650        660 
RVLAIQNEHI RARFDPDTGL LVEMENLDQN LLLPVRQAFY WYNASVGNNL STQVSGAYIF 

       670        680        690        700        710        720 
RPNQEKPLMV SHWAQTRLVK TPLVQEVHQN FSAWCSQVVR LYRGQRHLEL EWTVGPIPVG 

       730        740        750        760        770        780 
DGWGKEIISR FDTVLETKGL FYTDSNGREI LERRRDYRPT WKLNQTETVA GNYYPVNSRI 

       790        800        810        820        830        840 
YIRDGNMQLT VLTDRSQGGS SLRDGSMELM VHRRLLKDDG RGVGEALLED GLGRWVRGRH 

       850        860        870        880        890        900 
LVLLDKVRTA ATGHRLQAEK EVLTPQVVLA PGGGAPYHLK VAPRKQFSGL RRELPPSVHL 

       910        920        930        940        950        960 
LTLARWDQKT LLLRLEHQFA VGEDSGNLSS PVTLDLTDLF SAFTITYLQE TTLVANQLRA 

       970        980        990       1000 
SASRLKWTPN TGPTPLPSPS RLDPATITLQ PMEIRTFLAS VQWEEHG 

« Hide

References

[1]"Purification of feline lysosomal alpha-mannosidase, determination of its cDNA sequence and identification of a mutation causing alpha-mannosidosis in Persian cats."
Berg T., Tollersrud O.-K., Walkley S.U., Siegel D., Nilssen O.
Biochem. J. 328:863-870(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], DISEASE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF010191 mRNA. Translation: AAB97672.1.
AF010192 Genomic DNA. Translation: AAB97733.1.
PIRT42219.
RefSeqNP_001009222.1. NM_001009222.1.

3D structure databases

ProteinModelPortalO46432.
SMRO46432. Positions 52-342, 350-423, 433-582, 601-872, 883-1003.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH38. Glycoside Hydrolase Family 38.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID493697.
KEGGfca:493697.

Organism-specific databases

CTD4125.

Phylogenomic databases

HOVERGENHBG052391.
KOK12311.

Family and domain databases

Gene3D1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMA2B1_FELCA
AccessionPrimary (citable) accession number: O46432
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries