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Protein

Cytosolic purine 5'-nucleotidase

Gene

NT5C2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.1 Publication

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Enzyme regulationi

Allosterically activated by various compounds, including ATP.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei52 – 521NucleophileBy similarity
Metal bindingi52 – 521MagnesiumBy similarity
Active sitei54 – 541Proton donorBy similarity
Metal bindingi54 – 541Magnesium; via carbonyl oxygenBy similarity
Binding sitei127 – 1271Allosteric activator 1By similarity
Binding sitei154 – 1541Allosteric activator 2By similarity
Metal bindingi351 – 3511MagnesiumBy similarity
Binding sitei354 – 3541Allosteric activator 2By similarity
Binding sitei436 – 4361Allosteric activator 1; via carbonyl oxygenBy similarity
Binding sitei453 – 4531Allosteric activator 2By similarity

GO - Molecular functioni

  • 5'-nucleotidase activity Source: GO_Central
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.1.3.5. 908.
ReactomeiR-BTA-2161541. Abacavir metabolism.
R-BTA-74259. Purine catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic purine 5'-nucleotidase (EC:3.1.3.5)
Alternative name(s):
Cytosolic 5'-nucleotidase II
Cytosolic IMP/GMP-specific 5'-nucleotidase
Gene namesi
Name:NT5C2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 26

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560Cytosolic purine 5'-nucleotidasePRO_0000310263Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei502 – 5021PhosphoserineBy similarity
Modified residuei527 – 5271PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO46411.
PRIDEiO46411.

Expressioni

Gene expression databases

ExpressionAtlasiO46411. baseline.

Interactioni

Subunit structurei

Homotetramer.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-8487999,EBI-8487999

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017090.

Structurei

3D structure databases

ProteinModelPortaliO46411.
SMRiO46411. Positions 3-488.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni202 – 2109Substrate bindingSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi549 – 56012Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2469. Eukaryota.
ENOG410XQAV. LUCA.
GeneTreeiENSGT00550000074539.
HOGENOMiHOG000246075.
HOVERGENiHBG000025.
InParanoidiO46411.
KOiK01081.
OMAiGPEIRER.
OrthoDBiEOG7QK0BX.
TreeFamiTF315266.

Family and domain databases

Gene3Di3.40.50.1000. 3 hits.
InterProiIPR023214. HAD-like_dom.
IPR008380. HAD-SF_hydro_IG_5-nucl.
IPR016695. Pur_nucleotidase.
[Graphical view]
PANTHERiPTHR12103. PTHR12103. 1 hit.
PfamiPF05761. 5_nucleotid. 1 hit.
[Graphical view]
PIRSFiPIRSF017434. Purine_5'-nucleotidase. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02244. HAD-IG-Ncltidse. 1 hit.

Sequencei

Sequence statusi: Complete.

O46411-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSWSDRLQ NAADMPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG
60 70 80 90 100
FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL
110 120 130 140 150
VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF
160 170 180 190 200
YILNTLFNLP ETYLLACLVD FFTNCPRYTS CETGFKDGDL FMSYRSMFQD
210 220 230 240 250
VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN
260 270 280 290 300
SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT
310 320 330 340 350
VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTVCDLLG AKGKDILYIG
360 370 380 390 400
DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA
410 420 430 440 450
ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF
460 470 480 490 500
ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM
510 520 530 540 550
ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE
560
DDDEEEEEEE
Length:560
Mass (Da):64,841
Last modified:June 1, 1998 - v1
Checksum:i85E7CC64BF2581A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73690 mRNA. Translation: AAC48784.1.
RefSeqiNP_776830.1. NM_174405.2.
XP_005225491.1. XM_005225434.3.
UniGeneiBt.5382.

Genome annotation databases

EnsembliENSBTAT00000017090; ENSBTAP00000017090; ENSBTAG00000012858.
GeneIDi281951.
KEGGibta:281951.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73690 mRNA. Translation: AAC48784.1.
RefSeqiNP_776830.1. NM_174405.2.
XP_005225491.1. XM_005225434.3.
UniGeneiBt.5382.

3D structure databases

ProteinModelPortaliO46411.
SMRiO46411. Positions 3-488.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000017090.

Proteomic databases

PaxDbiO46411.
PRIDEiO46411.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000017090; ENSBTAP00000017090; ENSBTAG00000012858.
GeneIDi281951.
KEGGibta:281951.

Organism-specific databases

CTDi22978.

Phylogenomic databases

eggNOGiKOG2469. Eukaryota.
ENOG410XQAV. LUCA.
GeneTreeiENSGT00550000074539.
HOGENOMiHOG000246075.
HOVERGENiHBG000025.
InParanoidiO46411.
KOiK01081.
OMAiGPEIRER.
OrthoDBiEOG7QK0BX.
TreeFamiTF315266.

Enzyme and pathway databases

BRENDAi3.1.3.5. 908.
ReactomeiR-BTA-2161541. Abacavir metabolism.
R-BTA-74259. Purine catabolism.

Miscellaneous databases

NextBioi20805827.

Gene expression databases

ExpressionAtlasiO46411. baseline.

Family and domain databases

Gene3Di3.40.50.1000. 3 hits.
InterProiIPR023214. HAD-like_dom.
IPR008380. HAD-SF_hydro_IG_5-nucl.
IPR016695. Pur_nucleotidase.
[Graphical view]
PANTHERiPTHR12103. PTHR12103. 1 hit.
PfamiPF05761. 5_nucleotid. 1 hit.
[Graphical view]
PIRSFiPIRSF017434. Purine_5'-nucleotidase. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02244. HAD-IG-Ncltidse. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Bovine cytosolic IMP/GMP-specific 5'-nucleotidase: cloning and expression of active enzyme in Escherichia coli."
    Allegrini S., Pesi R., Tozzi M.G., Fiol C.J., Johnson R.B., Eriksson S.
    Biochem. J. 328:483-487(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Tissue: Thymus.

Entry informationi

Entry namei5NTC_BOVIN
AccessioniPrimary (citable) accession number: O46411
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: June 1, 1998
Last modified: April 13, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.